University of Crete

About: University of Crete is a education organization based out in Rethymno, Greece. It is known for research contribution in the topics: Population & Galaxy. The organization has 8681 authors who have published 21684 publications receiving 709078 citations. The organization is also known as: Panepistimio Kritis.

Inhibition of epithelial to mesenchymal transition in metastatic prostate cancer cells by the novel proteasome inhibitor, NPI-0052: pivotal roles of Snail repression and RKIP induction.

Abstract: Metastasis is associated with the loss of epithelial features and the acquisition of mesenchymal characteristics and invasive properties by tumor cells, a process known as epithelial to mesenchymal transition (EMT). Snail expression, through nuclear factor (NF)-κB activation, is an EMT determinant. The proteasome inhibitor, NPI-0052, induces the metastasis tumor suppressor/immune surveillance cancer gene, Raf kinase inhibitor protein (RKIP), via NF-κB inhibition. We hypothesized that NPI-0052 may inhibit Snail expression and, consequently, the metastatic phenotype in DU-145 prostate cancer cells. Cell treatment with NPI-0052 induced E-cadherin and inhibited Snail expression and both tumor cell invasion and migration. Inhibition of Snail inversely correlated with the induction of RKIP. The underlying mechanism of NPI-0052-induced inhibition of the metastatic phenotype was corroborated by: (1) treatment with Snail siRNA in DU-145 inhibited EMT and, in contrast, overexpression of Snail in the nonmetastatic LNCaP cells induced EMT, (2) NPI-0052-induced repression of Snail via inhibition of NF-κB was corroborated by the specific NF-κB inhibitor DHMEQ and (3) RKIP overexpression mimicked NPI-0052 in the inhibition of Snail and EMT. These findings demonstrate, for the first time, the role of NPI-0052 in the regulation of EMT via inhibition of NF-κB and Snail and induction of RKIP.

GOODS-Herschel: radio-excess signature of hidden AGN activity in distant star-forming galaxies

Abstract: Context. A tight correlation exists between far-infrared and radio emission for star-forming galaxies (SFGs), which seems to hold out to high redshifts (z {ap} 2). Any excess of radio emission over that expected from star formation processes is most likely produced by an active galactic nucleus (AGN), often hidden by large amounts of dust and gas. Identifying these radio-excess sources will allow us to study a population of AGN unbiased by obscuration and thus find some of the most obscured, Compton-thick AGN, which are in large part unidentified even in the deepest X-ray and infrared (IR) surveys. Aims: We present here a new spectral energy distribution (SED) fitting approach that we adopt to select radio-excess sources amongst distant star-forming galaxies in the GOODS-Herschel (North) field and to reveal the presence of hidden, highly obscured AGN. Methods: Through extensive SED analysis of 458 galaxies with radio 1.4 GHz and mid-IR 24 {$μ$}m detections using some of the deepest Chandra X-ray, Spitzer and Herschel infrared, and VLA radio data available to date, we have robustly identified a sample of 51 radio-excess AGN (~{}1300 deg$^{-2}$) out to redshift z {ap} 3. These radio-excess AGN have a significantly lower far-IR/radio ratio (q {lt} 1.68, 3{$σ$}) than the typical relation observed for star-forming galaxies (q {ap} 2.2). Results: We find that {ap}45% of these radio-excess sources have a dominant AGN component in the mid-IR band, while for the remainders the excess radio emission is the only indicator of AGN activity. The presence of an AGN is also confirmed by the detection of a compact radio core in deep VLBI 1.4 GHz observations for eight of our radio-excess sources ({ap}16%; {ap}66% of the VLBI detected sources in this field), with the excess radio flux measured from our SED analysis agreeing, to within a factor of two, with the radio core emission measured by VLBI. We find that the fraction of radio-excess AGN increases with X-ray luminosity reaching ~{}60% at L$_X$ {ap} 10$^{44}$ - 10$^{45}$ erg s$^{-1}$, making these sources an important part of the total AGN population. However, almost half (24/51) of these radio-excess AGN are not detected in the deep Chandra X-ray data, suggesting that some of these sources might be heavily obscured. Amongst the radio-excess AGN we can distinguish three groups of objects: i) AGN clearly identified in infrared (and often in X-rays), a fraction of which are likely to be distant Compton-thick AGN; ii) moderate luminosity AGN (L$_X$ {lsim} 10$^{43}$ erg s$^{-1}$) hosted in strong star-forming galaxies; and iii) a small fraction of low accretion-rate AGN hosted in passive (i.e. weak or no star-forming) galaxies. We also find that the specific star formation rates (sSFRs) of the radio-excess AGN are on average lower that those observed for X-ray selected AGN hosts, indicating that our sources are forming stars more slowly than typical AGN hosts, and possibly their star formation is progressively quenching. Tables 1, 3 and Appendices are available in electronic form at http://www.aanda.org

Mechanisms of the Development of Allergy (MeDALL): Introducing novel concepts in allergy phenotypes

Abstract: Asthma, rhinitis, and eczema are complex diseases with multiple genetic and environmental factors interlinked through IgE-associated and non-IgE-associated mechanisms. Mechanisms of the Development of ALLergy (MeDALL; EU FP7-CP-IP; project no: 261357; 2010-2015) studied the complex links of allergic diseases at the clinical and mechanistic levels by linking epidemiologic, clinical, and mechanistic research, including in vivo and in vitro models. MeDALL integrated 14 European birth cohorts, including 44,010 participants and 160 cohort follow-ups between pregnancy and age 20 years. Thirteen thousand children were prospectively followed after puberty by using a newly standardized MeDALL Core Questionnaire. A microarray developed for allergen molecules with increased IgE sensitivity was obtained for 3,292 children. Estimates of air pollution exposure from previous studies were available for 10,000 children. Omics data included those from historical genome-wide association studies (23,000 children) and DNA methylation (2,173), targeted multiplex biomarker (1,427), and transcriptomic (723) studies. Using classical epidemiology and machine-learning methods in 16,147 children aged 4 years and 11,080 children aged 8 years, MeDALL showed the multimorbidity of eczema, rhinitis, and asthma and estimated that only 38% of multimorbidity was attributable to IgE sensitization. MeDALL has proposed a new vision of multimorbidity independent of IgE sensitization, and has shown that monosensitization and polysensitization represent 2 distinct phenotypes. The translational component of MeDALL is shown by the identification of a novel allergic phenotype characterized by polysensitization and multimorbidity, which is associated with the frequency, persistence, and severity of allergic symptoms. The results of MeDALL will help integrate personalized, predictive, preventative, and participatory approaches in allergic diseases.

Heads or tails: host-parasite interactions in the Drosophila-Wolbachia system.

Abstract: Wolbachia strains are endosymbiotic bacteria typically found in the reproductive tracts of arthropods. These bacteria manipulate host reproduction to ensure maternal transmission. They are usually transmitted vertically, so it has been predicted that they have evolved a mechanism to target the host's germ cells during development. Through cytological analysis we found that Wolbachia strains display various affinities for the germ line of Drosophila. Different Wolbachia strains show posterior, anterior, or cortical localization in Drosophila embryos, and this localization is congruent with the classification of the organisms based on the wsp (Wolbachia surface protein) gene sequence. This embryonic distribution pattern is established during early oogenesis and does not change until late stages of embryogenesis. The posterior and anterior localization of Wolbachia resembles that of oskar and bicoid mRNAs, respectively, which define the anterior-posterior axis in the Drosophila oocyte. By comparing the properties of a single Wolbachia strain in different host backgrounds and the properties of different Wolbachia strains in the same host background, we concluded that bacterial factors determine distribution, while bacterial density seems to be limited by the host. Possible implications concerning cytoplasmic incompatibility and evolution of strains are discussed.