University of Extremadura

About: University of Extremadura is a education organization based out in Badajoz, Spain. It is known for research contribution in the topics: Population & Hyperspectral imaging. The organization has 7856 authors who have published 18299 publications receiving 396126 citations. The organization is also known as: Universidad de Extremadura.

Bmi1 regulates murine intestinal stem cell proliferation and self-renewal downstream of Notch.

Abstract: Genetic data indicate that abrogation of Notch-Rbpj or Wnt-β-catenin pathways results in the loss of the intestinal stem cells (ISCs). However, whether the effect of Notch is direct or due to the aberrant differentiation of the transit-amplifying cells into post-mitotic goblet cellsisunknown.Toaddressthisissue,wehavegeneratedcomposite tamoxifen-inducible intestine-specific genetic mouse models and analyzed the expression of intestinal differentiation markers. Importantly, we found that activation of β-catenin partially rescues thedifferentiationphenotypeofRbpjdeletionmutants,butnottheloss of the ISC compartment. Moreover, we identified Bmi1, which is expressed in the ISC and progenitor compartments, as a gene that is co-regulatedbyNotchand β-catenin.LossofBmi1resultedinreduced proliferation in the ISC compartment accompanied by p16 INK4a and p19 ARF (splice variants of Cdkn2a) accumulation, and increased differentiation to the post-mitotic goblet cell lineage that partially mimics Notch loss-of-function defects. Finally, we provide evidence that Bmi1 contributes to ISC self-renewal.

Autophagy and apoptosis have a role in the survival or death of stallion spermatozoa during conservation in refrigeration.

Abstract: Apoptosis has been recognized as a cause of sperm death during cryopreservation and a cause of infertility in humans, however there is no data on its role in sperm death during conservation in refrigeration; autophagy has not been described to date in mature sperm. We investigated the role of apoptosis and autophagy during cooled storage of stallion spermatozoa. Samples from seven stallions were split; half of the ejaculate was processed by single layer centrifugation, while the other half was extended unprocessed, and stored at 5°C for five days. During the time of storage, sperm motility (CASA, daily) and membrane integrity (flow cytometry, daily) were evaluated. Apoptosis was evaluated on days 1, 3 and 5 (active caspase 3, increase in membrane permeability, phosphatidylserine translocation and mitochondrial membrane potential) using flow cytometry. Furthermore, LC3B processing was investigated by western blotting at the beginning and at the end of the period of storage. The decrease in sperm quality over the period of storage was to a large extent due to apoptosis; single layer centrifugation selected non-apoptotic spermatozoa, but there were no differences in sperm motility between selected and unselected sperm. A high percentage of spermatozoa showed active caspase 3 upon ejaculation, and during the period of storage there was an increase of apoptotic spermatozoa but no changes in the percentage of live sperm, revealed by the SYBR-14/PI assay, were observed. LC3B was differentially processed in sperm after single layer centrifugation compared with native sperm. In processed sperm more LC3B-II was present than in non-processed samples; furthermore, in non-processed sperm there was an increase in LC3B-II after five days of cooled storage. These results indicate that apoptosis plays a major role in the sperm death during storage in refrigeration and that autophagy plays a role in the survival of spermatozoa representing a new pro-survival mechanism in spermatozoa not previously described.

Hyperspectral Unmixing Using Double Reweighted Sparse Regression and Total Variation

Abstract: Spectral unmixing is an important technique in hyperspectral image applications. Recently, sparse regression has been widely used in hyperspectral unmixing, but its performance is limited by the high mutual coherence of spectral libraries. To address this issue, a new sparse unmixing algorithm, called double reweighted sparse unmixing and total variation (TV), is proposed in this letter. Specifically, the proposed algorithm enhances the sparsity of fractional abundances in both spectral and spatial domains through the use of double weights, where one is used to enhance the sparsity of endmembers in spectral library, and the other is introduced to improve the sparsity of fractional abundances. Moreover, a TV-based regularization is further adopted to explore the spatial–contextual information. As such, the simultaneous utilization of both double reweighted $l_{1}$ minimization and TV regularizer can significantly improve the sparse unmixing performance. Experimental results on both synthetic and real hyperspectral data sets demonstrate the effectiveness of the proposed algorithm both visually and quantitatively.