Showing papers by "University of Fribourg published in 1994"

The Organization of the Chemosensory System in Drosophila Melanogaster: A Review

Abstract: This review surveys the organization of the olfactory and gustatory systems in the imago and in the larva of Drosophila melanogaster, both at the sensory and the central level. Olfactory epithelia of the adult are located primarily on the third antennal segment (funiculus) and on the maxillary palps. About 200 basiconic (BS), 150 trichoid (TS) and 60 coeloconic sensilla (CS) cover the surface of the funiculus, and an additional 60 BS are located on the maxillary palps. Males possess about 30% more TS but 20% fewer BS than females. All these sensilla are multineuronal; they may be purely olfactory or multimodal with an olfactory component. Antennal and maxillary afferents converge onto approximately 35 glomeruli within the antennal lobe. These projections obey precise rules: individual fibers are glomerulus-specific, and different types of sensilla are associated with particular subsets of glomeruli. Possible functions of antennal glomeruli are discussed. In contrast to olfactory sensilla, gustatory sensilla of the imago are located at many sites, including the labellum, the pharynx, the legs, the wing margin and the female genitalia. Each of these sensory sites has its own central target. Taste sensilla are usually composed of one mechano-and three chemosensory neurons. Individual chemosensory neurons within a sensillum respond to distinct subsets of molecules and project into different central target regions. The chemosensory system of the larva is much simpler and consists essentially of three major sensillar complexes on the cephalic lobe, the dorsal, terminal and ventral organs, and a series of pharyngeal sensilla.

Importance of unpredictability for reward responses in primate dopamine neurons

Abstract: 1. We used single neuron recording techniques in two behaving monkeys to investigate the conditions in which dopamine neurons respond to primary rewarding or potentially rewarding stimuli. Animals received drops of liquid either outside behavioral tasks or as rewards during learning or established performance of an auditory reaction time task. 2. Three quarters of dopamine neurons showed a short-latency, phasic response to liquid that was delivered outside the task without being predicted by phasic stimuli. The same neurons responded to liquid reward during learning but not when task performance was established, at which time the neuronal response occurred to the conditioned, reward-predicting, movement-triggering stimulus. 3. These data suggest that the responses of dopamine neurons to rewarding or potentially rewarding liquid are due to the temporally unpredicted stimulus occurrence. A known, reward-predicting, tonic context does not prevent dopamine neurons from responding to the rewarding liquid. The responses during learning apparently occur because reward is not yet reliably predicted by a conditioned phasic stimulus. Because the unpredicted occurrence of reward is of central importance for learning, these responses allow dopamine neurons to play an important role in reward-driven learning.

Transcallosal connections of the distal forelimb representations of the primary and supplementary motor cortical areas in macaque monkeys

Abstract: The goal of the present neuroanatomical study in macaque monkeys was twofold: (1) to clarify whether the hand representation of the primary motor cortex (M1) has a transcallosal projection to M1 of the opposite hemisphere; (2) to compare the topography and density of transcallosal connections for the hand representations of M1 and the supplementary motor area (SMA). The hand areas of M1 and the SMA were identified by intracortical microstimulation and then injected either with retrograde tracer substances in order to label the neurons of origin in the contralateral motor cortical areas (four monkeys) or, with an anterograde tracer, to establish the regional distribution and density of terminal fields in the opposite motor cortical areas (two monkeys). The main results were: (1) The hand representation of M1 exhibited a modest homotopic callosal projection, as judged by the small number of labeled neurons within the region corresponding to the contralateral injection. A modest heterotopic callosal projection originated from the opposite supplementary, premotor, and cingulate motor areas. (2) In contrast, the SMA hand representation showed a dense callosal projection to the opposite SMA. The SMA was found to receive also dense heterotopic callosal projections from the contralateral rostral and caudal cingulate motor areas, moderate projections from the lateral premotor cortex, and sparse projections from M1. (3) After injection of an anterograde tracer (biotinylated dextran amine) in the hand representation of M1, only a few small patches of axonal label were found in the corresponding region of M1, as well as in the lateral premotor cortex; virtually no label was found in the SMA or in cingulate motor areas. Injections of the same anterograde tracer in the hand representation of the SMA, however, resulted in dense and widely distributed axonal terminal fields in the opposite SMA, premotor cortex, and cingulate motor areas, while labeled terminals were clearly less dense in M1. It is concluded that the hand representations of the SMA and M1 strongly differ with respect to the strength and distribution of callosal connectivity with the former having more powerful and widespread callosal connections with a number of motor fields of the opposite cortex than the latter. These anatomical results support the proposition of the SMA being a bilaterally organized system, possibly contributing to bimanual coordination.

Complete Fermi surface mapping of Bi2Sr2CaCu2O8+x(001): Coexistence of short range antiferromagnetic correlations and metallicity in the same phase

Abstract: The Fermi surface of ${\mathrm{Bi}}_{2}$${\mathrm{Sr}}_{2}$${\mathrm{CaCu}}_{2}$${\mathrm{O}}_{8+\mathit{x}}$(001) has been mapped at nearly 6000 points in k space using angle-resolved photoemission at 300 K. We observe both features that are in good agreement with the local density calculation of Massidda, Yu, and Freeman [Physica (Amsterdam) 158C, 251 (1988)] and others that are missing in the calculation. Among those missing, most importantly, we find a c(2\ifmmode\times\else\texttimes\fi{}2) superstructure on the Fermi surface strongly suggesting short range antiferromagnetic correlations. It is remarkable that this is evident in the metallic state and in the Fermi surface itself.

Cerebellothalamocortical and pallidothalamocortical projections to the primary and supplementary motor cortical areas: A multiple tracing study in macaque monkeys

Abstract: The goal of the present study was to clarify whether the primary motor cortex (M1) and the supplementary motor cortex (SMA) both receive, via the motor thalamus, input from cerebellar and basal ganglia output nuclei. This is the first investigation that explores the problem by direct comparison, in the same animal, of thalamic zones that 1) project to M1 and SMA and 2) receive cerebellar-nuclear (CN) and pallidal (GP) afferents. These four zones were mapped in two monkeys by means of two retrograde tracers for M1 and SMA injections and of two anterograde tracers for CN and GP injections. All injections were performed under electrophysiological control (microstimulation and multiunit recordings). Injections in cortical areas were restricted to the hand/arm representation; in the SMA, the tracer deposit was within the "SMA-proper" (or "area F3") and did not include its rostral extension ("pre-SMA" or "area F6"). It was found that zones of all four types formed a number of highly complex patches of labeling that were usually not confined to one cytoarchitectonically defined thalamic nucleus. The overlap of clusters of labeled terminals and perikarya was evaluated morphometrically (area measurements) on a number of coronal sections along the anteroposterior extent of the motor thalamus. In line with previous studies, the thalamic territories innervated by CN and GP afferents rarely overlapped. However, zones projecting to M1 and/or to SMA included thalamic regions receiving CN as well as GP projections, providing the first evidence of such overlap from individual animals. The present observations support the previous conclusion from this laboratory (based on transsynaptic labeling) that the SMA receives, apart from its strong pallidal transthalamic input, a CN transthalamic input. These present findings that both M1 and SMA are recipients of transthalamic inputs from GP and CN thus support the concept that a mixed subcortical input consisting of weighted contributions from cerebellum, basal ganglia, substantia nigra, and spinothalamic tract is directed to each functional component of the sensorimotor cortex.

An analysis of regional climate change in Switzerland

Abstract: An analysis of daily climatological data covering the period from 1901 to 1992 for four locations in Switzerland (Zurich, Lugano, Davos, and Santis) has been made. The study has highlighted the fact that climate change this century is characterized by increases in minimum temperatures of about 2 K, a more modest increase in maximum temperatures (in some instances a decrease of maxima in the latter part of the record), little trend in the precipitation data, and a general decrease of sunshine duration through to the mid 1980s. The interannual variability is generally large, and filtering of the data to remove high-frequency noise shows that the regional climate undergoes a series of fluctuations of between 8 and 20 years' duration. The temperature change over this century is of greater magnitude than the global temperature changes published in the literature, reflecting an amplification of the global signal in the Alpine region; warming has been most intense in the 1940s, followed by the 1980s; the cooling which intervened from the 1950s to the late 1970s was not sufficient to offset the warming in the middle of the century. Pressure statistics have been compiled as a means of providing a link between the regional-scale climatological variables and the synoptic, supra-regional scale. These statistics show that pressure also exhibits a number of decadal-scale fluctuations, with the appearance of a new and anomalous behavior in the 1980s; in this decade, pressure reaches annual average values far higher than at other times this century. The pressure field is well correlated with the North Atlantic Oscillation (NAO) Index for distinct periods of the record (1931–1950 and 1971–1990) and is almost decorrelated from the NAO Index for the other decades of the century; this is indicative of transition from one climatic regime to another, dominated by zonal flow when the correlation with the NAO Index is high. In the 1980s, when zonal flow over the North Atlantic is strong, episodes of persistent, anomalously high pressures (blocking highs) are seen to occur over Switzerland, particularly during the winter season. The difference between the zonal and non-zonal regimes is particularly marked between the decade of the 1950s and that of the 1980s. The impact of this change between the 1950s and the 1980s on a number of climatological variables has been investigated statistically in order to provide an illustration of the manner in which changes in synoptic regimes (i.e., ‘climate change’) impacts upon climate characteristics on a regional scale. The analysis shows that temperature, precipitation, snow depth, and sunshine duration are indeed sensitive to large-scale influences; not only can yearly mean changes be quantified, but also seasonal and monthly fluctuations.

Platelet-derived growth factor-induced phosphatidylinositol 3-kinase activation mediates actin rearrangements in fibroblasts.

Abstract: Various agonist-induced cell responses in neutrophils and fibroblasts, such as chemotaxis and cytoskeletal rearrangements, have been shown to correlate with the synthesis of PtdIns(3,4,5)P3; however, the significance of this rise in second messenger levels is not clear. We show here that wortmannin inhibits platelet-derived growth factor (PDGF)-mediated production of PtdIns(3,4,5)P3 in human foreskin fibroblasts with an IC50 of about 5 nM. A similar inhibition was observed in in vitro assays (IC50 approximately 1 nM) with phosphatidylinositol 3-kinase immunoprecipitated by antibodies directed against the 85 kDa subunit (p85). On the other hand, wortmannin did not affect PDGF-mediated phosphorylation of p85 as detected by immunoprecipitation with anti-phosphotyrosine antibodies, and did not dissociate the complex of p85 and the catalytic subunit (p110) of phosphatidylinositol 3-kinase. These results are consistent with a direct, specific inhibition of the enzyme by wortmannin at concentrations relevant for its previously reported effects on cellular responses. When stimulated with PDGF, human foreskin fibroblasts form circular structures of filamentous actin. Preincubation of these cells with wortmannin inhibits PDGF-mediated actin rearrangements, suggesting a need for PtdIns(3,4,5)P3 formation as a signal for this cell response.

Time-dependent equations for phase differences and a collective mode in Josephson-coupled layered superconductors

Abstract: A time-dependent equation for the interlayer phase differences is derived for Josephson-coupled layered superconductors. It generalizes the sine-Gordon equation for the phase in a standard Josephson junction to the case of multilayer systems. With the help of this equation, the dispersion of a collective mode is found at low temperatures. In highly anisotropic systems the gap in the spectrum of this mode lies below the superconducting gap and is suppressed strongly by a magnetic field parallel to the layers. The effect of this mode on the dielectric function and specific heat is calculated.

S'imposer aux imposants. A propos de quelques obstacles rencontrés par des sociologues débutants dans la pratique et l'usage de l'entretien

Abstract: Chamboredon Helene, Pavis Fabienne, Surdez Muriel, Willemez Laurent. S'imposer aux imposants. A propos de quelques obstacles rencontres par des sociologues debutants dans la pratique et l'usage de l'entretien. In: Geneses, 16, 1994. Territoires urbains contestes, sous la direction de Michel Offerle. pp. 114-132.

Glycosylphosphatidylinositol membrane anchors in Saccharomyces cerevisiae: absence of ceramides from complete precursor glycolipids.

Abstract: Glycosylphosphatidylinositol (GPI) anchoring of membrane proteins occurs through two distinct steps, namely the assembly of a precursor glycolipid and its subsequent transfer onto newly synthesized proteins. To analyze the structure of the yeast precursor glycolipid we made use of the pmi40 mutant that incorporates very high amounts of [3H]mannose. Two very polar [3H]mannose-labeled glycolipids named CP1 and CP2 qualified as GPI precursor lipids since their carbohydrate head group, Man alpha 1,2(X-->PO4-->6)Man alpha 1,2Man alpha 1,6Man alpha-GlcN-inositol (with X most likely being ethanolamine) comprises the core structure which is common to all GPI anchors described so far. CP1 predominates in cells grown at 24 degrees C whereas CP2 is induced by stress conditions. The apparent structural identity of the head groups suggests that CP1 and CP2 contain different lipid moieties. The lipid moieties of both CP1 and CP2 can be removed by mild alkaline hydrolysis although the protein-bound GPI anchors made by the pmi40 cells under identical labeling conditions contain mild base resistant ceramides. These findings imply that the ceramide moiety found on the majority of yeast GPI anchored proteins is added through a lipid remodeling step that occurs after the addition of the GPI precursor glycolipids to proteins.

Discrete reduction patterns of parvalbumin and calbindin D-28k immunoreactivity in the dorsal lateral geniculate nucleus and the striate cortex of adult macaque monkeys after monocular enucleation

Abstract: We analyzed the immunohistochemical distribution of the two calcium-binding proteins, parvalbumin (PV) and calbindin D-28k (CB), in the primary visual cortex and lateral dorsal geniculate nucleus (dLGN) of monocularly enucleated macaque monkeys (Macaca fascicularis and Macaca nemestrina) in order to determine how the expression of PV and CB is affected by functional inactivity. The monkeys survived 1-17 weeks after monocular enucleation. The distribution pattern of each of the proteins was examined immunocytochemically using monoclonal antibodies and compared with that of the metabolic marker cytochrome oxidase (CO). We recorded manually the number of immunostained neurons and estimated the concentration of immunoreactive staining product using a computerized image-acquisition system. Our results indicate a decrease of approximately 30% in the labeling of PV-immunoreactive (ir) neuropil particularly in those layers of denervated ocular-dominance columns receiving the geniculocortical input. There was no change in the number of PV-ir neurons in any compartment irrespective of the enucleation interval. For CB-ir, we found a 20% decrease in the neuropil labeling in layer 2/3 of the denervated ocular-dominance columns. In addition, a subset of pyramidal CB-ir neurons in layers 2 and 4B, which are weakly stained in control animals, showed decreased labeling. In the dLGN of enucleated animals, PV-ir and CB-ir were decreased only in the neuropil of the denervated layers. From these results, we conclude that cortical interneurons and geniculate projection neurons still express PV and CB in their cell bodies after disruption of the direct functional input from one eye. The only distinct decrease of PV and CB expression is seen in axon terminals from retinal ganglion cells in the dLGN, and in the axons and terminals of both geniculocortical projection cells and cortical interneurons in the cerebral cortex.

Chapter 2: Moral Perspectives on Teaching

Abstract: In the middle of the last century, Adolf Diesterweg, a critical educational theorist, summarized what was expected from a competent teacher (see Dietrich, Elbing, Peagitsch, & Ritscher, 1983, p. 71): the health and strength of a Teuton, Lessing's penetrating mind, Hebbel's emotional depth, Pestalozzi's enthusiasm, Tillich's truthfulness, Salzman's eloquence, Leibniz's knowledge, Socrates's wisdom, and the love and charity of Jesus Christ. On February 11, 1993, the Swiss Teachers' Society developed a new guideline in which teachers are described as professionals for learning matters. They differ from laypersons in terms of the responsibility they show in providing children optimal opportunities for learning according to the modern knowledge of teaching; they engage in teamwork and have moral autonomy to create regional school cultures (decentralization); and they know how to deal with waywardness and violence in an affluent society. The teachers' society suggests an adaptation of the curriculum to new standards and pleads for differentiated ways of teaching in order to help each child to find his or her own way of learning. The society's guidelines are included in a paper titled "From the Public Education Priest to the Professional in a School Team" (Swiss Teachers' Society, 1993). What a difference in claims and expectations! What an ennoblement of teachers' roles and competences in earlier times, and what a technocratic description in modern times! Many of us, skeptical regarding claims for omnipotence in the teaching profession, struggle with the fact that we are made responsible for domains for which we cannot actually be responsible. Heid (1991), a German educational philosopher, explains that responsibility is connected to agency; it depends on being able to decide freely on one's acts and having the necessary knowledge—in both the planning phase and the phase of realizing a plan—for

Are There Unifying Structures in the Brain Responsible for Interlimb Coordination

Abstract: Publisher Summary This chapter discusses the problem of whether specific cortical areas are responsible for temporal and spatial coupling of both hands when they are engaged in purposeful cooperative manipulations, as is so often the case in the natural movement repertoire of primates. Many human skills are performed with the cooperation of both hands. A closer investigation, including a quantitative analysis of the temporospatial structure of the bilateral movement sequences, of such interesting cases remains to be done. More complex disturbances of bimanual performances have also been described in cases of medial frontal lesions, including the supplementary motor area. In multiarticulate limb movements and in speech articulation, it is found that despite a large trial-by-trial temporal and spatial variability of individual movement components, goal achievement is characterized by a remarkable spatial and temporal invariance.

Morphological indications for considerable diffuse reabsorption of cerebrospinal fluid in spinal meninges particularly in the areas of meningeal funnels. An electronmicroscopical study including tracing experiments in rats.

Abstract: Transmission and scanning electron microscopical observations in the rat indicate a considerable capacity of the spinal meninges to reabsorb cerebrospinal fluid. The density of blood vessels and lymphatics in the duramater is extremely high, particularly in the areas of meningeal funnels and spinal nerve root sleeves. Arterioles with closely related unmyelinated nerve fibres, many fenestrated capillaries and venules predetermine these areas as sites where absorption processes could take place. At certain sites of the meningeal angle region, the arachnoid membrane, mostly multilayered, is reduced to only three or four layers. Intercellular discontinuities and cytoplasmic fenestrations occurring in the arachnoid lining cell layer result in direct communications between the subarachnoid space and cisterns of the arachnoid “reticular layer”. These cisterns are partly fluid-filled, partly occupied by a net of collagen fibre bundles. Some cisterns harbour macrophages that often project filiform processes through the lining cell layer into the subarachnoid space, contacting cerebrospinal fluid. Desmosomes and gap junctions are present in all layers of the arachnoid. However, tight junctions and the continuous electrondense intercellular gap, known to occur normally within the “arachnoid barrier layer”, were not seen in many sites of the meningeal angle region. Numerous arachnoid cells display a high degree of vesiculation. Cationized ferritin, introduced in vivo into the rat subarachnoid space, passes inter- and intracellularly from the cerebrospinal fluid compartment through the arachnoid membrane, reaching durai blood vessels and lymphatics. Tracer could be visualized both in the cytoplasm of the endothelium and on the luminal surface of the cells. Tracer also passed through pial cell layers into pial vessels, through leptomeningeal sheaths into vessels crossing the subarachnoid space, into the connective tissue compartment and into vessels of spinal dorsal root ganglia. In the angle region, a particularly large number of macrophages can be found on the surface of leptomeninges, within the arachnoid reticular layers, and in close relation to dural and epidural capillaries, venules and lymphatics. Their possible role in the process of cerebrospinal fluid reabsorption is discussed.

Temporal structure of a bimanual goal-directed movement sequence in monkeys.

Abstract: The aim of the present investigation was to assess a bimanual goal-oriented movement sequence with particular emphasis on its temporal structure. The three monkeys (Macaca fascicularis) used in this study chose the left arm as the leading and more postural arm to reach out and pull back a spring-loaded drawer containing a food morsel. The right arm followed the left and picked up the food with a precision grip. Video recordings, trajectory recordings of the two index fingers, drawer displacement and the measurements of discrete events of the left and right hand revealed a considerable trial-by-trial variability in the temporal and spatial domain. The variability of latencies progressively increased from the initiation of the bimanual sequence to the left-hand and right-hand events defining goal achievement. The main result was that, in spite of this variability in each of the two limbs, there was an invariant left-right goal-related synchronization. The timing of the goal-related event pairs covaried and showed high correlation coefficients. Covariation of the two hands resulting in an invariant synchronization was particularly striking when monkeys performed the task without vision, and timing of right and left movement components was delayed with further increase in variability. The results indicate that, in the present bimanual skill, kinaesthetic signals may be sufficient to coordinate the two limbs in a goal-oriented unitary action in accord with a memorized plan.

Ribosomal heterogeneity from chromatin diminution in Ascaris lumbricoides

Abstract: The genome of Ascaris lumbricoides encodes both germline- and soma-specific proteins homologous to the eukaryotic small ribosomal protein (Rp) S19. The two Ascaris homologs differ by 24 amino acid substitutions and are both components of the small ribosomal subunits. In oocytes, the germline RpS19 homolog (RpS19G) predominates. During chromatin diminution, however, the gene is eliminated from all presomatic cells, and RpS19G is replaced by the product of the somatic gene (RpS19S). Chromatin diminution in A. lumbricoides causes a change in the protein composition of ribosomes during development and represents an alternative means of gene regulation.

Facile Preparation of (±)‐12‐Epiprostaglandins from 7‐Oxabicyclo[2.2.1]hept‐5‐en‐2‐one via an all‐cis‐formyllactone related to Corey lactone

Abstract: The bicyclic monoselenoacetal 7, easily obtained from (±)-7-oxabicyclo[2.2.1]hept-5-en-2-one (6) via a radical addition-acyl migration sequence, was converted to racemic 12-epiprostaglandins 3 and 4. The key intermediate was the all-cis-formyllactone 2b related to Corey lactone (see 12; Scheme 1). The presence of a (tert-butyl)-dimethylsilyl protective group for the 11-OH substituent (prostaglandin numbering) was found to be crucial in avoidingβ -elimination and epimerization during the Wittig-Horner reaction (Scheme 2). Epimerization at C(12) at the formyllactone stage (see 2b) was also possible and gave the known precursor 1b of naturally occurring prostaglandins and analogs.

Plasma treatment of polymers: the effect of the plasma parameters on the chemical, physical, and morphological states of the polymer surface and on the metal-polymer interface

Abstract: The results of the characterization of plasma-treated polypropylene (PP) and polymethylmethacrylate (PMMA) by X-ray photoelectron spectroscopic, atomic force microscopic (AFM), and surface resistivity measurements are presented. Chemical, physical, and morphological modifications of the polymer surface were investigated. The importance of the neutral gas of the electron cyclotron resonance-radio frequency (ECR-RF) plasma treatment is principally demonstrated. Large differences between the effects of noble and reactive gas plasmas were observed by AFM and surface resistivity measurements. However, the maximum sticking coefficient of evaporated Mg on PP is similar for both Ar and N2 plasma treatments.

Local structure of c(2×2)-Na on Al(001): Experimental evidence for the coexistence of intermixing and on-surface adsorption

Abstract: Angular distributions of x-ray-excited Na 1s photoelectrons and Na KVV Auger electrons emitted from c(2\ifmmode\times\else\texttimes\fi{}2)-Na/Al(001) have been measured over the full 2\ensuremath{\pi} solid angle above the Al(001) surface. A detailed study involving single-scattering cluster calculations and an R-factor analysis has been performed to determine the room-temperature adsorption site of Na on Al(001). The best agreement with experiment is obtained for a configuration in which domains of Na atoms adsorbed in the hollow site and in the substitutional site coexist on the surface. This unusual and unexpected behavior has not been observed for an alkali-metal-on-metal adsorption system up to now, but it can be understood on the basis of very recent results from first-principles density-functional theory [C. Stampfl, J. Neugebauer, and M. Scheffler, Surf. Rev. Lett. 1, 222 (1994)].