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Showing papers by "University of Fribourg published in 2002"


Journal ArticleDOI
10 Oct 2002-Neuron
TL;DR: Recent neurophysiological studies reveal that neurons in certain brain structures carry specific signals about past and future rewards, and the optimal use of rewards in voluntary behavior would benefit from interactions between the signals.

2,478 citations


Journal ArticleDOI
26 Jul 2002-Cell
TL;DR: The orphan nuclear receptor REV-ERBalpha is identified as the major regulator of cyclic Bmal1 transcription, and constitutes a molecular link through which components of the negative limb drive antiphasic expression of component of the positive limb.

2,052 citations


Journal ArticleDOI
TL;DR: In this paper, the authors used mRNA profiles generated from microarray experiments to deduce the functions of genes encoding known and putative Arabidopsis transcription factors, showing that transcription factors are important in regulating plant responses to environmental stress.
Abstract: Numerous studies have shown that transcription factors are important in regulating plant responses to environmental stress. However, specific functions for most of the genes encoding transcription factors are unclear. In this study, we used mRNA profiles generated from microarray experiments to deduce the functions of genes encoding known and putative Arabidopsis transcription factors. The mRNA levels of 402 distinct transcription factor genes were examined at different developmental stages and under various stress conditions. Transcription factors potentially controlling downstream gene expression in stress signal transduction pathways were identified by observed activation and repression of the genes after certain stress treatments. The mRNA levels of a number of previously characterized transcription factor genes were changed significantly in connection with other regulatory pathways, suggesting their multifunctional nature. The expression of 74 transcription factor genes responsive to bacterial pathogen infection was reduced or abolished in mutants that have defects in salicylic acid, jasmonic acid, or ethylene signaling. This observation indicates that the regulation of these genes is mediated at least partly by these plant hormones and suggests that the transcription factor genes are involved in the regulation of additional downstream responses mediated by these hormones. Among the 43 transcription factor genes that are induced during senescence, 28 of them also are induced by stress treatment, suggesting extensive overlap responses to these stresses. Statistical analysis of the promoter regions of the genes responsive to cold stress indicated unambiguous enrichment of known conserved transcription factor binding sites for the responses. A highly conserved novel promoter motif was identified in genes responding to a broad set of pathogen infection treatments. This observation strongly suggests that the corresponding transcription factors play general and crucial roles in the coordinated regulation of these specific regulons. Although further validation is needed, these correlative results provide a vast amount of information that can guide hypothesis-driven research to elucidate the molecular mechanisms involved in transcriptional regulation and signaling networks in plants.

991 citations


Journal ArticleDOI
TL;DR: A new mechanism leading to scale-free networks is proposed, which is called a good-get-richer mechanism, in which sites with larger fitness are more likely to become hubs (i.e., to be highly connected).
Abstract: A new mechanism leading to scale-free networks is proposed in this Letter. It is shown that, in many cases of interest, the connectivity power-law behavior is neither related to dynamical properties nor to preferential attachment. Assigning a quenched fitness value x(i) to every vertex, and drawing links among vertices with a probability depending on the fitnesses of the two involved sites, gives rise to what we call a good-get-richer mechanism, in which sites with larger fitness are more likely to become hubs (i.e., to be highly connected).

727 citations


Journal ArticleDOI
TL;DR: A review of the history, current status, physical mechanisms, experimental methods, and applications of nonlinear magneto-optical effects in atomic vapors can be found in this paper.
Abstract: The authors review the history, current status, physical mechanisms, experimental methods, and applications of nonlinear magneto-optical effects in atomic vapors. They begin by describing the pioneering work of Macaluso and Corbino over a century ago on linear magneto-optical effects (in which the properties of the medium do not depend on the light power) in the vicinity of atomic resonances. These effects are then contrasted with various nonlinear magneto-optical phenomena that have been studied both theoretically and experimentally since the late 1960s. In recent years, the field of nonlinear magneto-optics has experienced a revival of interest that has led to a number of developments, including the observation of ultranarrow (1-Hz) magneto-optical resonances, applications in sensitive magnetometry, nonlinear magneto-optical tomography, and the possibility of a search for parity- and time-reversal-invariance violation in atoms.

704 citations



Journal ArticleDOI
TL;DR: In this article, the authors investigated the capacity of single wall carbon nanotubes as a host material for hydrogen storage and found that the capacity depends linearly on the tube diameter and starts at 1.5 mass% for a 0.671 nm single wall nanotube.

531 citations


Journal ArticleDOI
01 Sep 2002-Ecology
TL;DR: A suite of food-web descriptors, which are built on information-theory indices and take the magnitude of the trophic interactions into account, are proposed and defined having a similar meaning as the classical qualitative indices.
Abstract: A food web customarily describes the qualitative feeding relationships in a community. Descriptors have been used to extract ecologically meaningful information from such data, e.g., the proportion of top species (the proportion of taxa without consumers) or vulnerability (the average number of consumers per taxon). Analyses of collections of food webs based on these properties have revealed regularities that fostered the formulation of models of food-web structure. However, it has been shown that most of these qualitative descriptors are highly sensitive to the varying levels of sampling effort used to document a food web. The principal problem is that webs described extensively include trophic links of highly uneven magnitude, with typically few strong/important links and a wealth of weak ones; with qualitative descriptors, the same weight is given to all trophic interactions. To overcome this problem, food webs should be described and analyzed quantitatively. Consequently, we propose here a suite of food-web descriptors, which are built on information-theory indices and take the magnitude of the trophic interactions into account. We define descriptors having a similar meaning as the classical qualitative indices. Two versions of each quantitative descriptor are proposed, one giving the same weight to each taxon, and one weighting each taxon by the total amount of its incoming and outgoing biomass flows. We use a published quantitative food web to exemplify the computation of the new descriptors, and discuss their potential and limitations.

487 citations


Journal ArticleDOI
TL;DR: Ten cDNAs representing all GST classes were cloned by RT-PCR and used to study AtGST expression in response to treatment with phytohormones, herbicides, oxidative stress and inoculation with virulent and avirulent strains of the downy mildew pathogen Peronospora parasitica, giving further insights into the complex regulation and enzymic functions of this plant gene super-family.
Abstract: Glutathione S-transferases (GSTs) appear to be ubiquitous in plants and have defined roles in herbicide detoxification. In contrast, little is known about their roles in normal plant physiology and during responses to biotic and abiotic stress. Forty-seven members of the GST super-family were identified in the Arabidopsis genome, grouped into four classes, with amino acid sequence identity between classes being below 25%. The two small zeta (GSTZ) and theta (GSTT) classes have related GSTs in animals while the large phi (GSTF) and tau (GSTU) classes are plant specific. As a first step to functionally characterize this diverse super-family, 10 cDNAs representing all GST classes were cloned by RT-PCR and used to study AtGST expression in response to treatment with phytohormones, herbicides, oxidative stress and inoculation with virulent and avirulent strains of the downy mildew pathogen Peronospora parasitica. The abundance of transcripts encoding AtGSTF9, AtGSTF10, AtGSTU5, AtGSTU13 and AtGSTT1 were unaffected by any of the treatments. In contrast, AtGSTF6 was upregulated by all treatments while AtGSTF2, AtGSTF8, AtGSTU19 and AtGSTZ1 each showed a selective spectrum of inducibility to the different stresses indicating that regulation of gene expression in this super-family is controlled by multiple mechanisms. The respective cDNAs were over expressed in E. coli. All GSTs except AtGSTF10 formed soluble proteins which catalysed a specific range of glutathione conjugation or glutathione peroxidase activities. Our results give further insights into the complex regulation and enzymic functions of this plant gene super-family.

486 citations


Journal ArticleDOI
TL;DR: It is demonstrated that Rho/ROCK pathway negatively regulates eNOS phosphorylation through inhibition of PKB, whereas it downregulates eNos expression independent of P KB.
Abstract: Endothelial nitric oxide synthase (eNOS) is an important regulator of cardiovascular homeostasis by production of nitric oxide (NO) from vascular endothelial cells. It can be activated by protein kinase B (PKB)/Akt via phosphorylation at Ser-1177. We are interested in the role of Rho GTPase/Rho kinase (ROCK) pathway in regulation of eNOS expression and activation. Using adenovirus-mediated gene transfer in human umbilical vein endothelial cells (HUVECs), we show here that both active RhoA and ROCK not only downregulate eNOS gene expression as reported previously but also inhibit eNOS phosphorylation at Ser-1177 and cellular NO production with concomitant suppression of PKB activation. Moreover, coexpression of a constitutive active form of PKB restores the phosphorylation but not gene expression of eNOS in the presence of active RhoA. Furthermore, we show that thrombin inhibits eNOS phosphorylation, as well as expression via Rho/ROCK pathway. Expression of the active PKB reverses eNOS phosphorylation but has no effect on downregulation of eNOS expression induced by thrombin. Taken together, these data demonstrate that Rho/ROCK pathway negatively regulates eNOS phosphorylation through inhibition of PKB, whereas it downregulates eNOS expression independent of PKB.

389 citations


Journal ArticleDOI
TL;DR: Analysis of different brain regions suggests that these proteins are involved in regulating calcium pools critical for synaptic plasticity, and a major role of any of these three calcium-binding proteins as an endogenous neuroprotectant is not generally supported.
Abstract: Calretinin (CR), calbindin D-28k (CB) and parvalbumin (PV) belong to the large family of EF-hand calcium-binding proteins, which comprises more than 200 members in man. Structurally these proteins are characterized by the presence of a variable number of evolutionary well-conserved helix-loop-helix motives, which bind Ca2+ ions with high affinity. Functionally, they fall into two groups: by interaction with target proteins, calcium sensors translate calcium concentrations into signaling cascades, whereas calcium buffers are thought to modify the spatiotemporal aspects of calcium transients. Although CR, CB and PV are currently being considered calcium buffers, this may change as we learn more about their biology. Remarkable differences in their biophysical properties have led to the distinction of fast and slow buffers and suggested functional specificity of individual calcium buffers. Evaluation of the physiological roles of CR, CB and PV has been facilitated by the recent generation of mouse strains deficient in these proteins. Here, we review the biology of these calcium-binding proteins with distinct reference to the cerebellum, since they are particularly enriched in specific cerebellar neurons. CR is principally expressed in granule cells and their parallel fibres, while PV and CB are present throughout the axon, soma, dendrites and spines of Purkinje cells. PV is additionally found in a subpopulation of inhibitory interneurons, the stellate and basket cells. Studies on deficient mice together with in vitro work and their unique cell type-specific distribution in the cerebellum suggest that these calcium-binding proteins have evolved as functionally distinct, physiologically relevant modulators of intracellular calcium transients. Analysis of different brain regions suggests that these proteins are involved in regulating calcium pools critical for synaptic plasticity. Surprisingly, a major role of any of these three calcium-binding proteins as an endogenous neuroprotectant is not generally supported.

Journal ArticleDOI
TL;DR: The results show a functional dissociation between the activations in the anterior frontomedian cortex and in the inferior precuneus, which was mainly activated by episodic retrieval processes, supporting its function as a multimodal association area that integrates the different aspects of retrieved and newly presented information.

Journal ArticleDOI
TL;DR: In this paper, a detailed account of the geological evolution along a western Swiss Alps transect is presented and confronted to the plate tectonic models derived from a larger western Tethys data base.
Abstract: Reconstructions of the Tethyan realm have been a matter of debate for a long time. Controversies spring usually from a lack of well constrained geological data which can be interpreted in different ways, or, from a lack of constraints on the plate tectonics framework. The western Alps region tends to escape these shortcomings due to a wealth of geological data regarding for examples the rifting and spreading of the Alpine Tethys and its closure, the formation of the Alpine orogenic wedge and accompanying foreland basins, through precise datation of these many events. The larger plate tectonic framework is also well constrained through precise positioning, at least since the Jurassic, of the main plates surrounding the western Alpine Tethys domain, such as the European and African plates, but also the Iberic and Adria micro-plates and the Alboran fragments. Therefore, the Western Alps and surrounding regions represent a key-stone for the western Tethys reconstruction. A detail account of the geological evolution along a western Swiss Alps transect is presented here and confronted to the plate tectonic models derived from a larger western Tethys data base.

Book ChapterDOI
01 Feb 2002
TL;DR: In this article, it was shown that the first Variscan orogenic event is the result of a collision between terranes detached from Gondwana (grouped as the Hun superterrane) and terrane detached from Eurasia.
Abstract: The well-known Variscan basement areas of Europe contain relic terranes with a pre-Variscan evolution testifying to their peri-Gondwanan origin (e.g., relics of Neoproterozoic volcanic arcs, and subsequent stages of accretionary wedges, backarc rifting, and spreading). The evolution of these terranes was guided by the diachronous subduction of the proto-Tethys oceanic ridge under different segments of the Gondwana margin. This subduction triggered the emplacement of magmatic bodies and the formation of backarc rifts, some of which became major oceanic realms (Rheic, paleoTethys). Consequently, the drifting of Avalonia was followed, after the Silurian and a short Ordovician orogenic event, by the drifting of Armorica and Alpine domains, accompanied by the opening of the paleo-Tethys. The slab rollback of the Rheic ocean is viewed as the major mechanism for the drifting of the European Variscan terranes. This, in turn, generated a large slab pull force responsible for the opening of major rift zones within the passive Eurasian margin. Therefore, the µrst Middle Devonian Variscan orogenic event is viewed as the result of a collision between terranes detached from Gondwana (grouped as the Hun superterrane) and terranes detached from Eurasia. Subsequently, the amalgamated terranes collided with Eurasia in a second Variscan orogenic event in Visean time, accompanied by large-scale lateral escape of major parts of the accreted margin. Final collision of Gondwana with Laurussia did not take place before Late Carboniferous time and was responsible for the Alleghanian orogeny.

Journal ArticleDOI
01 Mar 2002-Immunity
TL;DR: It is demonstrated that murine mast cell responses are exacerbated in vitro and in vivo by autocrine signals through G protein-coupled receptors (GPCRs) and require functional phosphoinositide 3-kinase γ (PI3Kγ), which relays inflammatory signals through various G i -coupling receptors and is central to mast cell function.

Journal ArticleDOI
TL;DR: Some of the recent advances in the understanding of systemic acquired resistance and on the role played by salicylic acid in this process are focused on.
Abstract: Plants can be induced to switch on defense reactions to a broad range of pathogens as a result of prior exposure to pathogens or to various chemicals or physical stress. Induced resistance is expressed locally, at the site of the infection or systemically, at sites remotely located from the initial infection. Upon recognition of the initial stimulus by the plant, a signal transduction pathway is set in motion, that includes intra and intercellular signals, and results in the activation of defense mechanisms, mostly by expression of new genes. This brief review will focus on some of the recent advances in the understanding of systemic acquired resistance and on the role played by salicylic acid in this process.

Journal ArticleDOI
TL;DR: The present findings are direct anatomical evidence for largely segregated visuomotor pathways linking parietal cortex with PMd and PMv.
Abstract: The lateral premotor cortex plays a crucial role in visually guided limb movements. Visual information may reach this cortical region from the parietal cortex, the highest stage in the dorsal visual stream. Anatomical studies indicate that the parietal projections to the dorsal (PMd) and ventral (PMv) premotor areas arise from separate parietal regions, supporting the notion of parallel visuomotor pathways. We tested the degree of segregation of these pathways by injecting retrograde tracers into PMd and PMv in the same monkeys, under physiological control. Eleven injections were made in four animals, and the analysis of retrograde labelling revealed that parietal cells projecting to PMd and those projecting to PMv are largely segregated. The strongest projections to PMd arise from the superior parietal lobule, including the medial intraparietal area (MIP), PEc and PGm, and the parieto-occipital area. These areas were devoid of labelling following injections into PMv, which receives its major projections from the anterior intraparietal area (AIP), area PEip, the anterior portion of the inferior parietal gyrus (area 7b), and the somatosensory areas. In addition to their strong projections to PMv, areas 7b and PEip send minor projections to PMd as well. Additional projections to PMd arise from the ventral intraparietal area and the inferior parietal lobule. The present findings are direct anatomical evidence for largely segregated visuomotor pathways linking parietal cortex with PMd and PMv.

Journal ArticleDOI
TL;DR: Results are consistent with the hypothesis that thiols regulate the flux through sulphate assimilation at the uptake and the APR step, and indicate that the flux control coefficient of APR for the pathway is proportionately less, with a significant share of the control exerted by the transport step.
Abstract: The effect of externally applied l-cysteine and glutathione (GSH) on ATP sulphurylase and adenosine 5′-phosphosulphate reductase (APR), two key enzymes of assimilatory sulphate reduction, was examined in Arabidopsis thaliana root cultures. Addition of increasing l-cysteine to the nutrient solution increased internal cysteine, γ-glutamylcysteine and GSH concentrations, and decreased APR mRNA, protein and extractable activity. An effect on APR could already be detected at 0.2 mm l-cysteine, whereas ATP sulphurylase was significantly affected only at 2 mm l-cysteine. APR mRNA, protein and activity were also decreased by GSH at 0.2 mm and higher concentrations. In the presence of l-buthionine-S, R-sulphoximine (BSO), an inhibitor of GSH synthesis, 0.2 mm l-cysteine had no effect on APR activity, indicating that GSH formed from cysteine was the regulating substance. Simultaneous addition of BSO and 0.5 mm GSH to the culture medium decreased APR mRNA, enzyme protein and activity. ATP sulphurylase activity was not affected by this treatment. Tracer experiments using 35SO42– in the presence of 0.5 mm l-cysteine or GSH showed that both thiols decreased sulphate uptake, APR activity and the flux of label into cysteine, GSH and protein, but had no effect on the activity of all other enzymes of assimilatory sulphate reduction and serine acetyltransferase. These results are consistent with the hypothesis that thiols regulate the flux through sulphate assimilation at the uptake and the APR step. Analysis of radioactive labelling indicates that the flux control coefficient of APR is more than 0.5 for the intracellular pathway of sulphate assimilation. This analysis also shows that the uptake of external sulphate is inhibited by GSH to a greater extent than the flux through the pathway, and that the flux control coefficient of APR for the pathway, including the transport step, is proportionately less, with a significant share of the control exerted by the transport step.

Journal ArticleDOI
TL;DR: Taken together, these data demonstrate that the light-induced signalling pathway interacts with the pathogen/SA-mediated signal transduction route.
Abstract: Summary The interaction of phytochrome signalling with the SA signal transduction pathway has been investigated in Arabidopsis using single and multiple mutants affected in light perception (phyA and phyB deficient) and light-signal processing (psi2, phytochrome signalling). The induction of PR1 by SA and functional analogues has been found to strictly correlate with the activity of the signalling pathway controlled by both phyA and phyB photoreceptors. In darkness as well as dim light, and independently of a carbohydrate source, SA-induced PR gene expression as well as the hypersensitive response to pathogens (HR) are strongly reduced. Moreover, the initiation of HR also exhibits a strict dependence upon both the presence and the amplitude of a phytochrome-elicited signal. The growth of an incompatible strain of bacterial a pathogen (Pseudomonas syringae pv. tomato) was enhanced in phyA-phyB and decreased in psi2 mutants. While functional chloroplasts were found necessary for the development of an HR, the induction of PRs was strictly dependent on light, but independent of functional chloroplasts. Taken together, these data demonstrate that the light-induced signalling pathway interacts with the pathogen/SA-mediated signal transduction route. These results are summarized in a formalism that allows qualitative computer simulation.

Journal ArticleDOI
18 Jan 2002-Science
TL;DR: In a natural Daphnia metapopulation in which genetic bottlenecks and local inbreeding are common, hybrid vigor amplifies the rate of gene flow several times more than would be predicted from the nominal migration rate.
Abstract: Immigrants to habitats occupied by conspecific organisms are usually expected to be competitively inferior, because residents may be locally adapted. If residents are inbred, however, mating between immigrants and residents results in offspring that may enjoy a fitness advantage from hybrid vigor. We demonstrate this effect experimentally in a natural Daphnia metapopulation in which genetic bottlenecks and local inbreeding are common. We estimate that in this metapopulation, hybrid vigor amplifies the rate of gene flow several times more than would be predicted from the nominal migration rate. This can affect the persistence of local populations and the entire metapopulation.

Journal ArticleDOI
TL;DR: Experimental evolution of learning ability in Drosophila melanogaster is reported, and it is demonstrated that behavioral changes are caused by the evolution of both a higher learning rate and a better memory.
Abstract: The presence of genetic variation for learning ability in animals opens the way for experiments asking how and under what ecological circumstances improved learning ability should evolve. Here we report experimental evolution of learning ability in Drosophila melanogaster. We exposed experimental populations for 51 generations to conditions that we expected to favor associative learning with regard to oviposition substrate choice. Flies that learned to associate a chemical cue (quinine) with a particular substrate, and still avoided this substrate several hours after the cue had been removed, were expected to contribute more alleles to the next generation. From about generation 15 on, the experimental populations showed marked ability to avoid oviposition substrates that several hours earlier had contained the chemical cue. The improved response to conditioning was also expressed when the flies were faced with a choice of novel media. We demonstrate that these behavioral changes are caused by the evolution of both a higher learning rate and a better memory.

Journal ArticleDOI
15 Mar 2002-Science
TL;DR: Genetic evidence is provided in Drosophila melanogaster for the paramount importance of the protein kinase Akt in mediating the effects of increased phosphatidylinositol 3,4,5-trisphosphate (PIP3) concentrations that are caused by the loss of PTEN function.
Abstract: The phosphoinositide phosphatase PTEN is mutated in many human cancers. Although the role of PTEN has been studied extensively, the relative contributions of its numerous potential downstream effectors to deregulated growth and tumorigenesis remain uncertain. We provide genetic evidence in Drosophila melanogaster for the paramount importance of the protein kinase Akt [also called protein kinase B (PKB)] in mediating the effects of increased phosphatidylinositol 3,4,5-trisphosphate (PIP3) concentrations that are caused by the loss of PTEN function. A mutation in the pleckstrin homology (PH) domain of Akt that reduces its affinity for PIP3 sufficed to rescue the lethality of flies devoid of PTEN activity. Thus, Akt appears to be the only critical target activated by increased PIP3 concentrations in Drosophila.

Journal ArticleDOI
TL;DR: It is shown that a biotic stress factor—attack by the oomycete pathogen Peronospora parasitica—can stimulate somatic recombination in Arabidopsis thaliana, and this findings suggest that increased somatics recombination is a general stress response in plants.
Abstract: Evolution is based on genetic variability and subsequent phenotypic selection. Mechanisms that modulate the rate of mutation according to environmental cues, and thus control the balance between genetic stability and flexibility, might provide a distinct evolutionary advantage. Stress-induced mutations stimulated by unfavorable environments, and possible mechanisms for their induction, have been described for several organisms, but research in this area has mainly focused on microorganisms. We have analyzed the influence of adverse environmental conditions on the genetic stability of the higher plant Arabidopsis thaliana. Here we show that a biotic stress factor-attack by the oomycete pathogen Peronospora parasitica-can stimulate somatic recombination in Arabidopsis. The same effect was observed when plant pathogen-defense mechanisms were activated by the chemicals 2,6-dichloroisonicotinic acid (INA) or benzothiadiazole (BTH), or by a mutation (cim3). Together with previous studies of recombination induced by abiotic factors, these findings suggest that increased somatic recombination is a general stress response in plants. The increased genetic flexibility might facilitate evolutionary adaptation of plant populations to stressful environments.

Journal ArticleDOI
TL;DR: Results show that oxygen-independent substrate level phosphorylation either linked to the citric acid cycle or tied into acetate production is essential for growth of procyclic T. brucei, a situation that may reflect an adaptation to the partially hypoxic conditions in the insect host.

Journal ArticleDOI
TL;DR: It is within this conceptual framework that this paper reviews the pathogenesis of obesity-related hypertension, which is highly complex and many factors act together to promote vasoconstriction and sodium retention.
Abstract: Obesity and arterial hypertension are important public health problems. Both overweight and hypertension predispose to cardiovascular diseases, such as myocardial infarction, stroke and renal failure. Moreover, overweight clearly predisposes to hypertension, and thus to an increased prevalence of cardiovascular diseases. This in turn favors inactivity and further weight gain, leading to an exacerbation of cardiovascular disorders. Obesity, hypertension and cardiovascular diseases thus contribute to three corners of a vicious triangle. It is within this conceptual framework that this paper reviews the pathogenesis of obesity-related hypertension, which is highly complex. Many factors act together to promote vasoconstriction and sodium retention. Leptin, free fatty acids and insulin, whose levels are increased in obesity, may act synergistically to stimulate sympathetic activity and vasoconstriction. In addition, obesity-induced insulin resistance and endothelial dysfunction may operate as amplifiers of the vasoconstrictor response. Finally, increased renal tubular reabsorption of sodium may also occur, caused by an increased renal sympathetic nerve activity, the direct effect of insulin, hyperactivity of the renin-angiotensin system and possibly by an alteration of intrarenal physical forces. All together, these factors will lead to sustained hypertension. Because the prevalence of obesity was steadily increasing in the last decades, leading to an increased prevalence of hypertension and cardiovascular disorders, obesity and hypertension will most likely become the health challenges of the twenty-first century.

Journal ArticleDOI
TL;DR: Genetic studies in Arabidopsis have shown that salicylic acid is made in the chloroplast from isochorismate, a pathway that is known to operate in prokaryotes.

Journal ArticleDOI
TL;DR: It is suggested that PtdInsP(3) is a second messenger for growth and that levels of Ptd insurance during development regulate organismal size.
Abstract: Understanding the control of size is of fundamental biological and clinical importance. Insulin/IGF signaling during development controls growth and size, possibly by coordinating the activities of the Ras and PI 3-kinase signaling pathways. We show that in Drosophila mutating the consensus binding site for the Ras pathway adaptor Drk/Grb2 in Chico/IRS does not interfere with growth whereas mutating the binding sites of the PI 3-kinase adaptor p60 completely abrogates Chico function. Furthermore, we present biochemical and genetic evidence that loss of the homolog of the tumor suppressor gene, Pten, results in increased PtdInsP(3) levels and that these increased levels are sufficient to compensate for the complete loss of the Insulin/insulin-like growth factor receptor function. This reduction of Pten activity is also sufficient to vastly increase organism size. These results suggest that PtdInsP(3) is a second messenger for growth and that levels of PtdInsP(3) during development regulate organismal size.

Journal ArticleDOI
TL;DR: In this paper, the interaction of atomic hydrogen and low-energy hydrogen ions with carbon networks was investigated on the surfaces of multilayer films, single-walled carbon nanotubes, and graphite.
Abstract: The interaction of atomic hydrogen and low-energy hydrogen ions with ${\mathrm{sp}}^{2}$-bonded carbon is investigated on the surfaces of ${\mathrm{C}}_{60}$ multilayer films, single-walled carbon nanotubes, and graphite (0001). These three materials have been chosen to represent ${\mathrm{sp}}^{2}$-bonded carbon networks with different local curvatures and closed surfaces (i.e. no dangling bonds). Chemisorption of hydrogen on these surfaces reduces emission from photoemission features associated with the \ensuremath{\pi} electrons and leads to a lowering of the work function up to 1.3 eV. It is found that the energy barrier for hydrogen adsorption decreases with increasing local curvature of the carbon surface. Whereas in the case of ${\mathrm{C}}_{60}$ and single-walled carbon nanotubes, hydrogen adsorption can be achieved by exposure to atomic hydrogen, the hydrogen adsorption on graphite (0001) requires ${H}^{+}$ ions of low kinetic energy (\ensuremath{\sim}1 eV). On all three materials, the adsorption energy barrier is found to increase with coverage. Accordingly, hydrogen chemisorption saturates at coverages that depend on the local curvature of the sample and the form of hydrogen (i.e., atomic or ionic) used for the treatment.

Journal ArticleDOI
TL;DR: The authors show that the circadian pattern of Per1 and Per2 gene expression in mice is modified under different photoperiods and that there is a mutual influence of these genes on their timing of expression.
Abstract: The Per1 and Per2 genes are components of the mammalian circadian clock. Mutations in these genes alter phase resetting in response to a nocturnal light pulse, and Per2 mutant mice are known to become arrhythmic in constant darkness. We show that under constant light conditions, Per2 mutant mice exhibit robust activity rhythms as well as body temperature rhythms with a period length that is less than 24 h. In Per1 mutants, the period length of both activity and body temperature rhythms is longer than 24 h in constant light. Per1 mutants prolong their period length (tao) when illuminance is increased, whereas Per2 mutants shorten their endogenous period. Additionally, the authors show that the circadian pattern of Per1 and Per2 gene expression in mice is modified under different photoperiods and that there is a mutual influence of these genes on their timing of expression. We propose that, in mice, the phase relationship between Per1 and Per2 gene expression might be critical for transducing day length information to the organism. Per1 could be part of a morning oscillator tracking dawn, and Per2 could be part of an evening oscillator tracking dusk.

Journal ArticleDOI
TL;DR: The magnetic properties of the interacting dimer system TlCuCl3 are investigated within a bond-operator formulation as a Bose condensation of magnons which are linear combinations of dimer singlet and triplet modes.
Abstract: The magnetic properties of the interacting dimer system ${\mathrm{T}\mathrm{l}\mathrm{C}\mathrm{u}\mathrm{C}\mathrm{l}}_{3}$ are investigated within a bond-operator formulation. The observed field-induced staggered magnetic order perpendicular to the field is described as a Bose condensation of magnons which are linear combinations of dimer singlet and triplet modes. This technique accounts for the magnetization curve and for the field dependence of the magnon dispersion curves observed by high-field neutron scattering measurements.