Showing papers by "University of Geneva published in 2001"
TL;DR: An increased alpha-SMA expression is sufficient to enhance fibroblast contractile activity, with the use of silicone substrates of different stiffness degrees.
Abstract: To evaluate whether alpha-smooth muscle actin (alpha-SMA) plays a role in fibroblast contractility, we first compared the contractile activity of rat subcutaneous fibroblasts (SCFs), expressing low levels of alpha-SMA, with that of lung fibroblasts (LFs), expressing high levels of alpha-SMA, with the use of silicone substrates of different stiffness degrees. On medium stiffness substrates the percentage of cells producing wrinkles was similar to that of alpha-SMA-positive cells in each fibroblast population. On high stiffness substrates, wrinkle production was limited to a subpopulation of LFs very positive for alpha-SMA. In a second approach, we measured the isotonic contraction of SCF- and LF-populated attached collagen lattices. SCFs exhibited 41% diameter reduction compared with 63% by LFs. TGFbeta1 increased alpha-SMA expression and lattice contraction by SCFs to the levels of LFs; TGFbeta-antagonizing agents reduced alpha-SMA expression and lattice contraction by LFs to the level of SCFs. Finally, 3T3 fibroblasts transiently or permanently transfected with alpha-SMA cDNA exhibited a significantly higher lattice contraction compared with wild-type 3T3 fibroblasts or to fibroblasts transfected with alpha-cardiac and beta- or gamma-cytoplasmic actin. This took place in the absence of any change in smooth muscle or nonmuscle myosin heavy-chain expression. Our results indicate that an increased alpha-SMA expression is sufficient to enhance fibroblast contractile activity.
1,186 citations
TL;DR: Pro- and anti-apoptotic members of the Bcl-2 family control the permeability of the outer mitochondrial membrane by forming autonomous pores in the membrane or by collaborating with components of the permeable transition pore.
Abstract: Pro- and anti-apoptotic members of the Bcl-2 family control the permeability of the outer mitochondrial membrane. They could do this either by forming autonomous pores in the membrane or by collaborating with components of the permeability transition pore. Here we discuss why we favour the first of these possibilities.
963 citations
TL;DR: Mesoporous semiconducting films consisting of preferentially orientated monoclinic-phase nanocrystals of tungsten trioxide have been prepared using a novel version of the sol-gel method, combining excellent photoresponse to the blue region of the solar spectrum, up to 500 nm, with good transparency at wavelengths larger than 550 nm.
Abstract: Mesoporous semiconducting films consisting of preferentially orientated monoclinic-phase nanocrystals of tungsten trioxide have been prepared using a novel version of the sol−gel method. Transformations undergone by a colloidal solution of tungstic acid, stabilized by an organic additive such as poly(ethylene glycol) (PEG) 300, as a function of the annealing temperature have been followed by means of a confocal Raman microscope. The shape and size of WO3 nanoparticles, the porosity, and the properties of the films depend critically on preparation parameters, such as the tungstic acid/PEG ratio, the PEG chain length, and the annealing conditions. Well-crystallized WO3 films combine excellent photoresponse to the blue region of the solar spectrum, up to 500 nm, with good transparency at wavelengths larger than 550 nm. Particular applications of these nanocrystalline WO3 films include photoelectrochemical and electrochromic devices.
961 citations
TL;DR: In this article, the G0 V star HD 166435 has been observed by the ber-fed spectrograph ELODIE as one of the targets in the large extra-solar planet survey that is conducting at the Observatory of Haute-Provence.
Abstract: The G0 V star HD 166435 has been observed by the ber-fed spectrograph ELODIE as one of the targets in the large extra-solar planet survey that we are conducting at the Observatory of Haute-Provence. We detected coherent, low-amplitude, radial-velocity variations with a period of 3.7987 days, suggesting a possible close-in planetary companion. Subsequently, we initiated a series of high-precision photometric observations to search for possible planetary transits and an additional series of Ca II H and K observations to measure the level of surface magnetic activity and to look for possible rotational modulation. Surprisingly, we found the star to be photometrically variable and magnetically active. A detailed study of the phase stability of the radial-velocity signal revealed that the radial-velocity variability remains coherent only for durations of about 30 days. Analysis of the time variation of the spectroscopic line proles using line bisectors revealed a correlation between radial velocity and line-bisector orientation. All of these observations, along with a one-quarter cycle phase shift between the photometric and the radial-velocity variations, are well explained by the presence of dark photospheric spots on HD 166435. We conclude that the radial-velocity variations are not due to gravitational interaction with an orbiting planet but, instead, originate from line-prole changes stemming from star spots on the surface of the star. The quasi-coherence of the radial-velocity signal over more than two years, which allowed a fair t with a binary model, makes the stability of this star unusual among other active stars. It suggests a stable magnetic eld orientation where spots are always generated at about the same location on the surface of the star.
906 citations
TL;DR: Elevated PCT concentrations appear to be a promising indicator of sepsis in newly admitted, critically ill patients capable of complementing clinical signs and routine laboratory parameters suggestive of severe infection.
Abstract: To assess the diagnostic value of procalcitonin (PCT), interleukin (IL)-6, IL-8, and standard measurements in identifying critically ill patients with sepsis, we performed prospective measurements in 78 consecutive patients admitted with acute systemic inflammatory response syndrome (SIRS) and suspected infection. We estimated the relevance of the different parameters by using multivariable regression modeling, likelihood-ratio tests, and area under the receiver operating characteristic curves (AUC). The final diagnosis was SIRS in 18 patients, sepsis in 14, severe sepsis in 21, and septic shock in 25. PCT yielded the highest discriminative value, with an AUC of 0.92 (CI, 0.85 to 1.0), followed by IL-6 (0.75; CI, 0.63 to 0.87), and IL-8 (0.71; CI, 0.59 to 0.83; p < 0.001). At a cutoff of 1.1 ng/ml, PCT yielded a sensitivity of 97% and a specificity of 78% to differentiate patients with SIRS from those with sepsis-related conditions. Median PCT concentrations on admission (ng/ ml, range) were 0.6 (0 to 5.3) for SIRS; 3.5 (0.4 to 6.7) for sepsis; 6.2 (2.2 to 85) for severe sepsis; and 21.3 (1.2 to 654) for septic shock (p < 0.001). The addition of PCT to a model based solely on standard indicators improved the predictive power of detecting sepsis (likelihood ratio test; p = 0.001) and increased the AUC value for the routine value-based model from 0.77 (CI, 0.64 to 0.89) to 0.94 (CI, 0.89 to 0.99; p = 0.002). In contrast, no additive effect was seen for IL-6 (p = 0.56) or IL-8 (p = 0.14). Elevated PCT concentrations appear to be a promising indicator of sepsis in newly admitted, critically ill patients capable of complementing clinical signs and routine laboratory parameters suggestive of severe infection.
875 citations
TL;DR: Organelles in the endocytic pathway are composed of a mosaic of structural and functional regions, indicating that membrane organization might be modular.
Abstract: Organelles in the endocytic pathway are composed of a mosaic of structural and functional regions. These regions consist, at least in part, of specialized protein-lipid domains within the plane of the membrane, or of protein complexes associated with specific membrane lipids. Whereas some of these molecular assemblies can be found in more than one compartment, a given combination seems to be unique to each compartment, indicating that membrane organization might be modular.
742 citations
TL;DR: The assumption that mechanical tension is crucial for myofibroblast modulation and for the maintenance of their contractile activity is supported.
Abstract: We have examined the role of mechanical tension in myofibroblast differentiation using two in vivo rat models. In the first model, granulation tissue was subjected to an increase in mechanical tension by splinting a full-thickness wound with a plastic frame. Myofibroblast features, such as stress fiber formation, expression of ED-A fibronectin and α-smooth muscle actin (α-SMA) appeared earlier in splinted than in unsplinted wounds. Myofibroblast marker expression decreased in control wounds starting at 10 days after wounding as expected, but persisted in splinted wounds. In the second model, granuloma pouches were induced by subcutaneous croton oil injection; pouches were either left intact or released from tension by evacuation of the exudate at 14 days. The expression of myofibroblast markers was reduced after tension release in the following sequence: F-actin (2 days), α-SMA (3 days), and ED-A fibronectin (5 days); cell density was not affected. In both models, isometric contraction of tissue strips was measured after stimulation with smooth muscle agonists. Contractility correlated always with the level of α-SMA expression, being high when granulation tissue had been subjected to tension and low when it had been relaxed. Our results support the assumption that mechanical tension is crucial for myofibroblast modulation and for the maintenance of their contractile activity.
632 citations
TL;DR: The advantages and shortcomings of the performance measures currently used in CBIR are discussed and proposals for a standard test suite similar to that used in IR at the annual Text REtrieval Conference (TREC), are presented.
598 citations
TL;DR: In this article, a preclinical and human vaccine studies indicate that, although neonatal immunisation does not generally lead to rapid and strong antibody responses, it may result in an efficient immunological priming, which can serve as an excellent basis for future responses.
596 citations
TL;DR: Alcohol- based hand rubs may be better than traditional handwashing as they require less time, act faster, are less irritating, and contribute to sustained improvement in compliance associated with decreased infection rates.
Abstract: Hand hygiene prevents cross-infection in hospitals, but health-care workers' adherence to guidelines is poor. Easy, timely access to both hand hygiene and skin protection is necessary for satisfactory hand hygiene behavior. Alcohol- based hand rubs may be better than traditional handwashing as they require less time, act faster, are less irritating, and contribute to sustained improvement in compliance associated with decreased infection rates. This article reviews barriers to appropriate hand hygiene and risk factors for noncompliance and proposes strategies for promoting hand hygiene.
581 citations
TL;DR: The property of pathogenic mycobacteria to cause phagosome maturation arrest in infected macrophages is applied as a tool to dissect critical steps in phagosomal biogenesis, reporting the requirement for 3-phosphoinositides and acquisition of Rab5 effector early endosome autoantigen (EEA1) as essential molecular events necessary for phagosomic maturation.
Abstract: Phagosomal biogenesis is a fundamental biological process of particular significance for the function of phagocytic and antigen-presenting cells. The precise mechanisms governing maturation of phagosomes into phagolysosomes are not completely understood. Here, we applied the property of pathogenic mycobacteria to cause phagosome maturation arrest in infected macrophages as a tool to dissect critical steps in phagosomal biogenesis. We report the requirement for 3-phosphoinositides and acquisition of Rab5 effector early endosome autoantigen (EEA1) as essential molecular events necessary for phagosomal maturation. Unlike the model phagosomes containing latex beads, which transiently recruited EEA1, mycobacterial phagosomes excluded this regulator of vesicular trafficking that controls membrane tethering and fusion processes within the endosomal pathway and is recruited to endosomal membranes via binding to phosphatidylinositol 3-phosphate (PtdIns[3]P). Inhibitors of phosphatidylinositol 3′(OH)-kinase (PI-3K) activity diminished EEA1 recruitment to newly formed latex bead phagosomes and blocked phagosomal acquisition of late endocytic properties, indicating that generation of PtdIns(3)P plays a role in phagosomal maturation. Microinjection into macrophages of antibodies against EEA1 and the PI-3K hVPS34 reduced acquisition of late endocytic markers by latex bead phagosomes, demonstrating an essential role of these Rab5 effectors in phagosomal biogenesis. The mechanism of EEA1 exclusion from mycobacterial phagosomes was investigated using mycobacterial products. Coating of latex beads with the major mycobacterial cell envelope glycosylated phosphatidylinositol lipoarabinomannan isolated from the virulent Mycobacterium tuberculosis H37Rv, inhibited recruitment of EEA1 to latex bead phagosomes, and diminished their maturation. These findings define the generation of phosphatidylinositol 3-phosphate and EEA1 recruitment as: (a) important regulatory events in phagosomal maturation and (b) critical molecular targets affected by M. tuberculosis. This study also identifies mycobacterial phosphoinositides as products with specialized toxic properties, interfering with discrete trafficking stages in phagosomal maturation.
TL;DR: A mathematical function to evaluate closeness relationships and classify the study area into homogeneous zones was used, compatible with the outranking function of ELECTRE-TRI used to assess the suitability index and are very useful in dealing with inherent conflicts in land-use planning.
Abstract: Land-use planners often make complex decisions within a short period of time when they must take into account sustainable development and economic competitiveness. A set of land-use suitability maps would be very useful in this respect. Ideally, these maps should incorporate complex criteria integrating several stakeholders' points of view. To illustrate the feasibility of this approach, a land suitability map for housing was realised for a small region of Switzerland. Geographical Information System technology was used to assess the criteria requested to define the suitability of land for housing. An example dealing with the evaluation of noise levels illustrates the initial steps of this procedure. Because the required criteria are heterogeneous and measured on various scales, an outranking multicriteria analysis method called ELECTRE-TRI was used. However, using it to assess the suitability of any point in a territory was impractical due to computational limitations. Therefore, a mathematical function ...
TL;DR: It is demonstrated that Balb/c mice lacking the GABA(B(1)) subunit are viable, exhibit spontaneous seizures, hyperalgesia, hyperlocomotor activity, and memory impairment, and casts doubt on the existence of proposed receptor subtypes.
TL;DR: Three different in vitro methods, specifically, classic diffusion cell studies, attenuated total-reflectance-Fourier transform infrared spectroscopy, and tape stripping in conjunction with an appropriate analytical technique, are considered, emphasizing their application to obtain quantitative values for skin transport parameters and to separate the kinetic or thermodynamic effects of an enhancement strategy.
TL;DR: The study of RFX and CIITA has made major contributions to the comprehension of the molecular mechanisms controlling MHCII genes and has made this system into a textbook model for the regulation of gene expression.
Abstract: The bare lymphocyte syndrome (BLS) is a hereditary immunodeficiency resulting from the absence of major histocompatibility complex class II (MHCII) expression. Considering the central role of MHCII molecules in the development and activation of CD4(+) T cells, it is not surprising that the immune system of the patients is severely impaired. BLS is the prototype of a "disease of gene regulation." The affected genes encode RFXANK, RFX5, RFXAP, and CIITA, four regulatory factors that are highly specific and essential for MHCII genes. The first three are subunits of RFX, a trimeric complex that binds to all MHCII promoters. CIITA is a non-DNA-binding coactivator that functions as the master control factor for MHCII expression. The study of RFX and CIITA has made major contributions to our comprehension of the molecular mechanisms controlling MHCII genes and has made this system into a textbook model for the regulation of gene expression.
TL;DR: It is demonstrated, for the first time, that the increase of SWS need is under a strong genetic control, and they provide a basis for identifying genes underlying SWS homeostasis.
Abstract: Delta power, a measure of EEG activity in the 1-4 Hz range, in slow-wave sleep (SWS) is in a quantitative and predictive relationship with prior wakefulness. Thus, sleep loss evokes a proportional increase in delta power, and excess sleep a decrease. Therefore, delta power is thought to reflect SWS need and its underlying homeostatically regulated recovery process. The neurophysiological substrate of this process is unknown and forward genetics might help elucidate the nature of what is depleted during wakefulness and recovered during SWS. We applied a mathematical method that quantifies the relationship between the sleep-wake distribution and delta power to sleep data of six inbred mouse strains. The results demonstrated that the rate at which SWS need accumulated varied greatly with genotype. This conclusion was confirmed in a "dose-response" study of sleep loss and changes in delta power; delta power strongly depended on both the duration of prior wakefulness and genotype. We followed the segregation of the rebound of delta power after sleep deprivation in 25 BXD recombinant inbred strains by quantitative trait loci (QTL) analysis. One "significant" QTL was identified on chromosome 13 that accounted for 49% of the genetic variance in this trait. Interestingly, the rate at which SWS need decreases did not vary with genotype in any of the 31 inbred strains studied. These results demonstrate, for the first time, that the increase of SWS need is under a strong genetic control, and they provide a basis for identifying genes underlying SWS homeostasis.
TL;DR: In this article, the photoelectrochemical characteristics of highly transparent nanoporous WO3 films are described and the photocurrent versus excitation wavelength spectra exhibit a maximum close to 400 nm and a significant photoresponse to the blue part of the visible spectrum.
Abstract: The photoelectrochemical characteristics of highly transparent nanoporous WO3 films are described. The photocurrent versus excitation wavelength spectra of these photoelectrodes exhibit a maximum close to 400 nm and a significant photoresponse to the blue part of the visible spectrum. The observed conversion efficiencies attain 75% for the photogeneration of oxygen from 1 M aq HClO4 and reach 190% in the presence of methanol in the solution, denoting in the latter case the occurrence of a perfect photocurrent doubling. Experiments conducted under simulated solar AM 1.5 illumination resulted in steady-state anodic photocurrents of the order of several mA/cm2.
TL;DR: It is shown that the phase resetting in peripheral clocks of nocturnal mice is slow when feeding time is changed from night to day and rapid when switched back from day to night.
Abstract: The circadian timing system in mammals is composed of a master pacemaker in the suprachiasmatic nucleus (SCN) of the hypothalamus and slave clocks in most peripheral cell types. The phase of peripheral clocks can be completely uncoupled from the SCN pacemaker by restricted feeding. Thus, feeding time, while not affecting the phase of the SCN pacemaker, is a dominant Zeitgeber for peripheral circadian oscillators. Here we show that the phase resetting in peripheral clocks of nocturnal mice is slow when feeding time is changed from night to day and rapid when switched back from day to night. Unexpectedly, the inertia in daytime feeding-induced phase resetting of circadian gene expression in liver and kidney is not an intrinsic property of peripheral oscillators, but is caused by glucocorticoid signaling. Thus, glucocorticoid hormones inhibit the uncoupling of peripheral and central circadian oscillators by altered feeding time.
TL;DR: It is found that early and late origins of replication are highly mobile in G1 phase, frequently moving at or faster than 0.5 micrometers/10 seconds, in an energy-dependent fashion, and telomeres and centromeres provide replication-independent constraint on chromatin movement in both G1 and S phases.
Abstract: Little is known about the dynamics of chromosomes in interphase nuclei. By tagging four chromosomal regions with a green fluorescent protein fusion to lac repressor, we monitored the movement and subnuclear position of specific sites in the yeast genome, sampling at short time intervals. We found that early and late origins of replication are highly mobile in G1 phase, frequently moving at or faster than 0.5 micrometers/10 seconds, in an energy-dependent fashion. The rapid diffusive movement of chromatin detected in G1 becomes constrained in S phase through a mechanism dependent on active DNA replication. In contrast, telomeres and centromeres provide replication-independent constraint on chromatin movement in both G1 and S phases.
TL;DR: In this article, a grid of models with and without the eects of axial rotation for massive stars in the range of 9t o 60M and metallicity Z = 0 :004 appropriate for the SMC was calculated.
Abstract: We calculate a grid of models with and without the eects of axial rotation for massive stars in the range of 9t o 60M and metallicity Z =0 :004 appropriate for the SMC. Remarkably, the ratios =crit of the angular velocity to the break{up angular velocity grow strongly during the evolution of high mass stars, contrary to the situation at Z =0 :020. The reason is that at low Z, mass loss is smaller and the removal of angular momentum during evolution much weaker, also there is an ecient outward transport of angular momentum by meridional circulation. Thus, a much larger fraction of the stars at lower Z reach break{up velocities and rotation may thus be a dominant eect at low Z. The models with rotation well account for the long standing problem of the large numbers of red supergiants observed in low Z galaxies, while current models with mass loss were predicting no red supergiants. We discuss in detail the physical eects of rotation which favour a redwards evolution in the HR diagram. The models also predict large N enrichments during the evolution of high mass stars. The predicted relative N{enrichments are larger at Z lower than solar and this is in very good agreement with the observations for A{type supergiants in the SMC.
TL;DR: This overview is targeted at ICU physicians to convince them that the principles of infection control in the ICU are based on simple concepts and that the application of preventive strategies should not be viewed as an administrative or constraining control of their activity but, rather, as basic measures that are easy to implement at the bedside.
TL;DR: In a rodent model of genetic obesity, the fa/fa Zucker diabetic fatty rat, the mechanisms involved in the most common complications of diet‐induced human obesity, i.e., noninsulin‐dependent diabetes mellitus, and myocardial dysfunction are reviewed.
Abstract: Obesity-related diseases now threaten to reach epidemic proportions in the United States. Here we review in a rodent model of genetic obesity, the fa/fa Zucker diabetic fatty (ZDF) rat, the mechanisms involved in the most common complications of diet-induced human obesity, i.e., noninsulin-dependent diabetes mellitus, and myocardial dysfunction. In ZDF rats, hyperphagia leads to hyperinsulinemia, which up-regulates transcription factors that stimulate lipogenesis. This causes ectopic deposition of triacylglycerol in nonadipocytes, providing fatty acid (FA) substrate for damaging pathways of nonoxidative metabolism, such as ceramide synthesis. In beta cells and myocardium, the resulting functional impairment and apoptosis cause diabetes and cardiomyopathy. Interventions that lower ectopic lipid accumulation or block nonoxidative metabolism of FA and ceramide formation completely prevent these complications. Given the evidence for a similar etiology for the complications of human obesity, it would be appropriate to develop strategies to avert the predicted epidemic of lipotoxic disorders.
TL;DR: It is shown that the lowest moments of fractional Brownian motion are actually all identical, except the second moment of the velocity, which provides a simple criterion that enable us to distinguish these two non-Markovian processes.
Abstract: We investigate fractional Brownian motion with a microscopic random-matrix model and introduce a fractional Langevin equation. We use the latter to study both subdiffusion and superdiffusion of a free particle coupled to a fractal heat bath. We further compare fractional Brownian motion with the fractal time process. The respective mean-square displacements of these two forms of anomalous diffusion exhibit the same power-law behavior. Here we show that their lowest moments are actually all identical, except the second moment of the velocity. This provides a simple criterion that enable us to distinguish these two non-Markovian processes.
TL;DR: The findings presented here reveal unpredicted roles for PPARα and β in adult mouse epidermal repair and PPARβ mutant primary keratinocytes show impaired adhesion and migration properties.
Abstract: We show here that the alpha, beta, and gamma isotypes of peroxisome proliferator-activated receptor (PPAR) are expressed in the mouse epidermis during fetal development and that they disappear progressively from the interfollicular epithelium after birth. Interestingly, PPARalpha and beta expression is reactivated in the adult epidermis after various stimuli, resulting in keratinocyte proliferation and differentiation such as tetradecanoylphorbol acetate topical application, hair plucking, or skin wound healing. Using PPARalpha, beta, and gamma mutant mice, we demonstrate that PPARalpha and beta are important for the rapid epithelialization of a skin wound and that each of them plays a specific role in this process. PPARalpha is mainly involved in the early inflammation phase of the healing, whereas PPARbeta is implicated in the control of keratinocyte proliferation. In addition and very interestingly, PPARbeta mutant primary keratinocytes show impaired adhesion and migration properties. Thus, the findings presented here reveal unpredicted roles for PPARalpha and beta in adult mouse epidermal repair.
TL;DR: In this article, the authors studied the mechanism of the oxygen reduction reaction on platinum nanoparticles supported on carbon inside Nafion ® (i.e. in PEMFC cathode conditions).
TL;DR: It is proposed that azithromycin interferes with the synthesis of autoinducers, by an unknown mechanism, leading to a reduction of virulence factor production.
Abstract: We report that 2 microg of azithromycin/ml inhibits the quorum-sensing circuitry of Pseudomonas aeruginosa strain PAO1. Addition of synthetic autoinducers partially restored the expression of the trancriptional activator-encoding genes lasR and rhlR but not that of the autoinducer synthase-encoding gene lasI. We propose that azithromycin interferes with the synthesis of autoinducers, by an unknown mechanism, leading to a reduction of virulence factor production.
TL;DR: In this paper, a canonical final sigma(E) binding site could be revealed upon mapping transcriptional start sites and identifying 20 E coli promoters which activate reporter gene transcription in a sigma-dependent manner.
TL;DR: The data show that some sera may contain more than one type of MSA and confirm the strong association of anti-Ro52 with anti-Jo-1 reactivity, which may be explained by the use of multiple recombinant fragments spanning the complete antigen.
Abstract: Objective—To determine the prevalence of myositis specific autoantibodies (MSAs) and several myositis associated autoantibodies (MAAs) in a large group of patients with myositis. Methods—A total of 417 patients with myositis from 11 European countries (198 patients with polymyositis (PM), 181 with dermatomyositis (DM), and 38 with inclusion body myositis (IBM)) were serologically analysed by immunoblot, enzyme linked immunosorbent assay (ELISA) and/or immunoprecipitation. Results—Autoantibodies were found in 232 sera (56%), including 157 samples (38%) which contained MSAs. The most commonly detected MSA was anti-Jo-1 (18%). Other anti-synthetase, anti-Mi-2, and antiSRP autoantibodies were found in 3%, 14%, and 5% of the sera, respectively. A relatively high number of anti-Mi-2 positive PM sera were found (9% of PM sera). The most commonly detected MAA was anti-Ro52 (25%). Anti-PM/Scl-100, antiPM/Scl-75, anti-Mas, anti-Ro60, anti-La, and anti-U1 snRNP autoantibodies were present in 6%, 3%, 2%, 4%, 5%, and 6% of the sera, respectively. Remarkable associations were noticed between anti-Ro52 and anti-Jo-1 autoantibodies and, in a few sera, also between anti-Jo-1 and anti-SRP or anti-Mi-2 autoantibodies. Conclusions—The incidence of most of the tested autoantibody activities in this large group of European patients is in agreement with similar studies of Japanese and American patients. The relatively high number of PM sera with anti-Mi-2 reactivity may be explained by the use of multiple recombinant fragments spanning the complete antigen. Furthermore, our data show that some sera may contain more than one type of MSA and confirm the strong association of anti-Ro52 with anti-Jo-1 reactivity. (Ann Rheum Dis 2001;60:116‐123)
TL;DR: In this article, the authors present a setup for quantum secret sharing based on energy-time entanglement, which takes advantage of only two entangled photons created via parametric down conversion.
Abstract: We present a setup for quantum secret sharing based on energy-time entanglement. In opposition to known implementations using three particle Greenberger-Horne-Zeilinger ~GHZ! states, our idea takes advantage of only two entangled photons created via parametric down conversion. However, the system comprising the pump plus the two down-converted photons bare the same quantum correlation and can be used to mimic three entangled qubits. The relatively high coincidence count rates found in our setup enable for the first time an application of a quantum communication protocol based on more than two qubits.
TL;DR: In this paper, the volume expansion of spatially averaged portions of inhomogeneous cosmologies in spacetimes filled with an arbitrary fluid is investigated and two different averaging schemes and corresponding systems of averaged evolution equations providing generalizations of Papers I and II are presented.
Abstract: We investigate effective equations governing the volume expansion of spatially averaged portions of inhomogeneous cosmologies in spacetimes filled with an arbitrary fluid. This work is a follow-up to previous studies focused on irrotational dust models (Paper I) and irrotational perfect fluids (Paper II) in flow-orthogonal foliations of spacetime. It complements them by considering arbitrary foliations, arbitrary lapse and shift, and by allowing for a tilted fluid flow with vorticity. As for the first studies, the propagation of the spatial averaging domain is chosen to follow the congruence of the fluid, which avoids unphysical dependencies in the averaged system that is obtained. We present two different averaging schemes and corresponding systems of averaged evolution equations providing generalizations of Papers I and II. The first one retains the averaging operator used in several other generalizations found in the literature. We extensively discuss relations to these formalisms and pinpoint limitations, in particular regarding rest mass conservation on the averaging domain. The alternative averaging scheme that we subsequently introduce follows the spirit of Papers I and II and focuses on the fluid flow and the associated 1+3 threading congruence, used jointly with the 3+1 foliation that builds the surfaces of averaging. This results in compact averaged equations with a minimal number of cosmological backreaction terms. We highlight that this system becomes especially transparent when applied to a natural class of foliations which have constant fluid proper time slices.