University of Guelph

About: University of Guelph is a education organization based out in Guelph, Ontario, Canada. It is known for research contribution in the topics: Population & Gene. The organization has 26542 authors who have published 50553 publications receiving 1715255 citations. The organization is also known as: U of G & Guelph University.

Osmosensing by Bacteria: Signals and Membrane-Based Sensors

Abstract: Bacteria can survive dramatic osmotic shifts. Osmoregulatory responses mitigate the passive adjustments in cell structure and the growth inhibition that may ensue. The levels of certain cytoplasmic solutes rise and fall in response to increases and decreases, respectively, in extracellular osmolality. Certain organic compounds are favored over ions as osmoregulatory solutes, although K+ fluxes are intrinsic to the osmoregulatory response for at least some organisms. Osmosensors must undergo transitions between “off” and “on” conformations in response to changes in extracellular water activity (direct osmosensing) or resulting changes in cell structure (indirect osmosensing). Those located in the cytoplasmic membranes and nucleoids of bacteria are positioned for indirect osmosensing. Cytoplasmic membrane-based osmosensors may detect changes in the periplasmic and/or cytoplasmic solvent by experiencing changes in preferential interactions with particular solvent constituents, cosolvent-induced hydration changes, and/or macromolecular crowding. Alternatively, the membrane may act as an antenna and osmosensors may detect changes in membrane structure. Cosolvents may modulate intrinsic biomembrane strain and/or topologically closed membrane systems may experience changes in mechanical strain in response to imposed osmotic shifts. The osmosensory mechanisms controlling membrane-based K+ transporters, transcriptional regulators, osmoprotectant transporters, and mechanosensitive channels intrinsic to the cytoplasmic membrane of Escherichia coli are under intensive investigation. The osmoprotectant transporter ProP and channel MscL act as osmosensors after purification and reconstitution in proteoliposomes. Evidence that sensor kinase KdpD receives multiple sensory inputs is consistent with the effects of K+ fluxes on nucleoid structure, cellular energetics, cytoplasmic ionic strength, and ion composition as well as on cytoplasmic osmolality. Thus, osmoregulatory responses accommodate and exploit the effects of individual cosolvents on cell structure and function as well as the collective contribution of cosolvents to intracellular osmolality.

Macronutrient metabolism by the human gut microbiome: major fermentation by-products and their impact on host health

Abstract: The human gut microbiome is a critical component of digestion, breaking down complex carbohydrates, proteins, and to a lesser extent fats that reach the lower gastrointestinal tract. This process results in a multitude of microbial metabolites that can act both locally and systemically (after being absorbed into the bloodstream). The impact of these biochemicals on human health is complex, as both potentially beneficial and potentially toxic metabolites can be yielded from such microbial pathways, and in some cases, these effects are dependent upon the metabolite concentration or organ locality. The aim of this review is to summarize our current knowledge of how macronutrient metabolism by the gut microbiome influences human health. Metabolites to be discussed include short-chain fatty acids and alcohols (mainly yielded from monosaccharides); ammonia, branched-chain fatty acids, amines, sulfur compounds, phenols, and indoles (derived from amino acids); glycerol and choline derivatives (obtained from the breakdown of lipids); and tertiary cycling of carbon dioxide and hydrogen. Key microbial taxa and related disease states will be referred to in each case, and knowledge gaps that could contribute to our understanding of overall human wellness will be identified.

High frequency haploid production in barley (Hordeum vulgare L.).

Abstract: HAPLOIDS have been sought and investigated in many plant species since their first identification in Datura1. The chief limitation in using them to produce homozygous lines for plant breeding programmes or genetic research has been the lack of techniques by which large numbers of haploids can be obtained from any given stock2. Reports3,4 of haploid plants obtained from anther cultures offer a potential technique and here we report our first observations on interspecific crosses, followed by embryo culture, illustrating an equally promising method for obtaining haploids in barley and perhaps other species.

Yield Improvement in Temperate Maize is Attributable to Greater Stress Tolerance

Abstract: A retrospective analysis of the physiological basis of genetic yield improvement may provide an understanding of yield potential and may indicate avenues for future yield improvement. Rate of yield improvement of maize (Zea mays L.) hybrids in Ontario, Canada has been ≈1.5% yr⁻¹ during the last five decades. Comparison of short-season hybrids representing yield improvement from the late 1950s to the late 1980s showed that genetic yield improvement was 2.5% per year and that most of the genetic yield improvement could be attributed to increased stress tolerance. Differences in stress tolerance between older and more recent hybrids have been shown for high plant population density, weed interference, low night temperatures during the grain-filling period, low soil moisture, low soil N, and a number of herbicides. Yield improvement is the result of more efficient capture and use of resources, and the improved efficiency in resource capture and use of newer hybrids is frequently only evident under stress. Improved resource capture has resulted from increased interception of seasonal incident radiation and greater uptake of nutrients and water. The improved resource capture is associated with increased leaf longevity, a more active root system, and a higher ratio of assimilate supply by the leaf canopy (source) and assimilate demand by the grain (sink) during the grain-filling period. Improvements of resource use under optimum conditions have been small, as leaf photosynthesis, leaf-angle distribution of the canopy, grain chemical composition, and the proportion of dry matter allocated to the grain at maturity (i.e., harvest index) have remained virtually constant. Genetic improvement of maize has been accompanied by a decrease in plant-to-plant variability. Results of our studies indicate that increased stress tolerance is associated with lower plant-to-plant variability and that increased plant-to-plant variability results in lower stress tolerance.