Showing papers by "University of Hawaii at Manoa published in 1970"
TL;DR: Sequences of the polynucleotide chains of RNA found in the large and small ribosomal subunits of rabbit reticulocytes have been determined from the 3′-end by use of periodate oxidation and condensation with [3H]isoniazid and by stepwise degradation.
Abstract: Sequences of the polynucleotide chains of RNA found in the large and small ribosomal subunits of rabbit reticulocytes have been determined from the 3′-end by use of periodate oxidation and condensation with [3H]isoniazid and by stepwise degradation. By these methods the hexanucleotide sequences have been found as -pGpUpUpUpGpU for the 28S RNA and -pGpUpCpGpCpU for the 6S RNA of the large ribosomal subunit and the octanucleotide sequence -pGpApUpCpApUpUpA for the 18S rRNA of the small ribosomal subunit. These sequences are present in at least 70% of all the RNA molecules and are discussed in relation to the specific cleavage of rRNA from its precursors and the role of multiple cistrons for rRNA in the DNA of higher organisms. The feasibility of using the method for longer sequence determinations is discussed.
52 citations
TL;DR: The hyperkinetic syndrome is one of many manifestations of minimal brain dysfunction and emotional distrubance and both physical and psychologic factors can be seen in its etiology.
Abstract: The hyperkinetic syndrome is one of many manifestations of minimal brain dysfunction and emotional distrubance. Both physical and psychologic factors can be seen in its etiology. Depression has often been found to be related to hyperkinesis. The children described in this study give ample support to the frequency of this relationship. Some preliminary hypotheses are also drawn about the dynamics of the relationship between depression and the hyperkinetic syndrome.
13 citations
TL;DR: Data are presented which suggest that the RNA from rabbit reticulocytes consists of two main subunits of molecular weight 0.65 and 1.53 × 106 daltons and that in most conditions of isolation there is a small molecule of RNA associated in equimolar amounts with the large RNA subunit.
Abstract: THE molecular weight of mammalian ribosomal RNA has been the subject of various studies. Estimates of molecular weight of RNA by sedimentation and viscosity from Jensen sarcoma1, liver2 and HeLa cell3 ribosomes are between 0.58 and 0.67 × 106 daltons for the small subunit and between 1.64 and 1.9 × 106 daltons for the large subunit. Estimates made by measuring the length of RNA molecules are 0.86 and 0.83 and 1.73 and 1.61 × 106 daltons for the small and large subunits of erythroblast and HeLa cell rRNA when compared with the length of Escherichia coli rRNA4. Estimates made by exonuclease digestion, when compared with E. coli RNA, give values of 0.4 and 0.8 × 106 daltons for the small and large subunits of HeLa cell rRNA5. I now present data which suggest that the RNA from rabbit reticulocytes consists of two main subunits of molecular weight 0.65 and 1.53 × 106 daltons and that in most conditions of isolation there is a small molecule of RNA associated in equimolar amounts with the large RNA subunit.
12 citations
TL;DR: A column-chromatographic system using DEAE-cellulose and gradient elution with triethylammonium formate at pH4.0-3.5 is described, capable of separating the oligonucleotide isonicotinoyl hydrazones that are produced by nuclease digestion of RNA oxidized with periodate and coupled withIsonicotinic acid hydrazide.
Abstract: A column-chromatographic system using DEAE-cellulose and gradient elution with triethylammonium formate at pH4.0–3.5 is described. It is capable of separating the oligonucleotide isonicotinoyl hydrazones that are produced by nuclease digestion of RNA oxidized with periodate and coupled with isonicotinic acid hydrazide. Fifteen dinucleoside monophosphate isonicotinoyl hydrazones were characterized by their elution positions on the columns, so that all but two of them could readily be identified. Twelve trinucleoside diphosphate hydrazones were also characterized by their elution positions on the column. The application of this method of fractionation to terminal-sequence studies of RNA is discussed.
3 citations