Showing papers by "University of Hohenheim published in 1983"
TL;DR: In this paper, a rapid method was described for the colorimetric determination of 1.5-15 μg phytate phosphorus in concentrations as low as 3 μg ml−1 in extracts of cereal grains and cereal products.
Abstract: A rapid method is described for the colorimetric determination of 1.5–15 μg phytate phosphorus in concentrations as low as 3 μg ml−1 in extracts of cereal grains and cereal products. The phytic acid is precipitated with an acidic iron-III-solution of known iron content. The decrease of iron in the supernatant is a measure for the phyticacid content.
883 citations
TL;DR: The results demonstrate the extent to which the pH in the rhizosphere can differ from that further from the roots, depending on plant species and nitrogen source.
399 citations
TL;DR: The results of these experiments support the idea of an enzymic reduction of Fe(III) on the plasmalemma of cortical cells of roots.
Abstract: Iron deficiency in peanuts (Arachis hypogeae L.) caused an increase in release of caffeic acid, a higher rate of Fe(III) reduction, and increased rates of both Fe(III) chelate splitting and iron uptake.Experiments on Fe(III) reduction by phenolics (in vitro experiments) and by roots of Fe-deficient peanuts exclude the direct involvement of released phenolics in Fe(III) reduction by roots: Fe(III) reduction by phenolics had a pH optimum higher than 8.0 and was strongly dependent on the concentration and the stability of the supplied Fe(III) chelates. In contrast, Fe(III) reduction by roots of Fe-deficient peanuts had a pH optimum of about 5.0 and was less dependent on the stability of the supplied Fe(III) chelates. Furthermore, the observed release of phenolics into nutrient solution would have to be at least 200 times higher to attain the reduction rates of roots of Fe-deficient peanuts. The results of these experiments support the idea of an enzymic reduction of Fe(III) on the plasmalemma of cortical cells of roots.
386 citations
TL;DR: Inhibition of root elongation, the most sensitive parameter for the toxic effect of Al on the growth of cowpea (Vigna unguicalata), was mainly the result of inhibited cell division in the root-tip meristems.
128 citations
114 citations
TL;DR: A method is described for the determination of protein degradation based on measurements of ammonia concentration and gas production when a feedingstuff was incubated with rumen fluid in vitro.
Abstract: A method is described for the determination of protein degradation based on measurements of ammonia concentration and gas production (Menke et al. 1979) when a feedingstuff was incubated with rumen fluid in vitro. NH3 liberated during incubation is in part used for microbial protein synthesis. Production of carbon dioxide and methane can be regarded as a measure of energy available for protein synthesis. The ratio, gas production: incorporation of NH3-nitrogen was estimated by addition of starch to the substrate. The response in gas production was linear in the range 0-200 mg starch, when starch was added to 0-200 mg feedingstuff dry matter and 30 ml rumen fluid-medium mixture. Linear regression between NH3-N concentration (y, mg) and gas production (x, ml) yielded an intercept (bo) representing that amount of NH3-N which would be released when no fermentable carbohydrates were available and consequently no bacterial protein synthesis took place. The difference between this intercept bo and NH3-N content in the blank (rumen fluid without substrate added) indicated the amount of NH3 liberated from protein and other N-containing compounds of the feeding-stuff incubated. In vitro-degradable N (IVDN) was calculated as a proportion of total N by the equation: (formula; see text).
106 citations
TL;DR: A peptide antibiotic has been isolated from Trichoderma reesei QM 9414 as discussed by the authors, which is crystalline and uniform in TLC, this antibiotic could be resolved by HPLC into 3 sequence analogues.
Abstract: A peptide antibiotic has been isolated from Trichoderma reesei QM 9414. Although crystalline and uniform in TLC, this antibiotic could be resolved by HPLC into 3 sequence analogues. The close relationship to alamethicin was proved by chemical and spectroscopic methods, and the formation of ion-conducting pores in lipid bilayers.
98 citations
TL;DR: Pollination of flowers of standard carnation with pollen from flowers of miniature carnations caused them to wilt irreversibly within 1 to 2 days, and there appears to be a qualitative difference between the enzyme in the stigmas converting ACC to ethylene in other parts of the flower.
Abstract: Pollination of flowers of standard carnation (Dianthus caryophyllus L. cv. White Sim) with pollen from flowers of miniature carnations (D. caryophyllus L. cv. Exquisite) caused them to wilt irreversibly within 1 to 2 days. Pollination stimulated a sequential increase in ethylene production by stigmas, ovaries, receptacles, and petals of the flowers. The ACC content of the stigmas increased rapidly in the first few hours after pollination. The possibility that subsequent production of ethylene by other parts of the flower is stimulated by translocated ACC is discussed. Ethylene production and ACC content of other parts of the flower reached their maximum 24 h after pollination. The petal tissues contributed the bulk of the ethylene productionper flower thereafter. There appears to be a qualitative difference between the enzyme in the stigmas converting ACC to ethylene and that in other parts of the flower.
98 citations
TL;DR: It is suggested that the increased proton secretion under iron deficiency is the main function of rhizodermal transfer cells.
88 citations
TL;DR: In this paper, a reversed-phase system for the separation of simple phenolics and related substances is described, which consists of a LiChrosorb RP-18 (10 μm) Knauer column and a combination of isocratic and linear gradient elution (solvent a, water-formic acid (95:5); solvent B, methanol; temperature, 35°C).
77 citations
TL;DR: In boars on an artificial light programme the off-season depression during the summer was completely restored and indicates that daylight fluctuations are the main reason for seasonal changes of testicular function in the domestic boar.
TL;DR: The degradation of ochratoxin A is described by a Gram-negative soil bacterium that grows at the expense of phenylalanine and was therefore tested for its ability to utilize the phenylAlanine moiety of o chratoxins.
Abstract: Ochratoxins are formed as products of secondary metabolism in some species of Aspergillus [1] and Penicillium [2]. These fungi are widely distributed in nature and have been isolated from decaying vegetation, soils and foodstuffs. Ochratoxin A causes nephropathic effects in animals [3] and also gives rise to growth inhibition of Gram-posit ive bacteria [4]. In this paper we describe the degradation of ochratoxin A by a Gram-negative soil bacterium. This bacterium grows at the expense of phenylalanine and was therefore tested for its ability to utilize the phenylalanine moiety of ochratoxin A. Ochratoxin A was a gift from Prof. Dr. R. R~Sschenthaler (MOnster), Sephadex G I 0 and LH20 were from Pharmacia. All other chemicals were from Merck AG (Darmstadt).
01 Sep 1983
TL;DR: In this article, the authors reviewed sampling and analytical techniques for the measurement and characterization of intracellular muscle free amino acids and relevant studies concerning the functions of these amino acids are summarized, and unique patterns of these acids in different catabolic states and interpretations and hypotheses of these patterns as related to protein catabolism are discussed.
Abstract: Sampling and analytical techniques for the measurement and characterization of intracellular muscle free amino acids are reviewed, and relevant studies concerning the functions of these amino acids are summarized. The unique patterns of these acids in different catabolic states and interpretations and hypotheses of these patterns as related to protein catabolism are discussed. Special attention also is given to the effect of nutritional regimens on these acids. (wz)
TL;DR: In experiments with 29 cowpea genotypes considerable variation in Mn tolerance could be found, but ranking according to Mn tolerance was almost the same in sand and water culture.
Abstract: In experiments with 29 cowpea genotypes considerable variation in Mn tolerance could be found. Ranking according to Mn tolerance was almost the same in sand and water culture. Mn tolerance is not related to greater vigour or exclusion of Mn from uptake and translocation, but depends mainly on the internal tolerance to excess Mn especially in the leaf tissue.
TL;DR: Results demonstrated that gastric emptying was regulated by several co-operating factors, particularly the pylorus, which was involved by producing different resistances to flow during its phase of relaxation.
Abstract: The motility of the antrum, duodenum and pylorus was recorded with strain gauge transducers and induction coils in conscious beagle dogs. The effect of oleic acid on gastroduodenal motility and on the pyloric diameter was studied in the interdigestive state and during emptying of a mashed potato meal. The flow of digesta was observed fluoroscopically. Gastric emptying was measured by planimetry of the radiopaque gastric silhouette. The intraduodenal injection of oleic acid reduced the diameter of the pylorus in the empty stomach. In the digestive state oleic acid diminished gastric emptying of the potato meal. The antral motility was significantly diminished (amplitudes from 100 +/- 39.2 to 93 +/- 41.2 mV, contractile frequency from 4.3 +/- 0.6 to 3.8 +/- 1.5 min-1, motility index from 435 +/- 197 to 345 +/- 213 V, n = 3400-4700 contractions), whereas the duodenal motility was enhanced (amplitudes from 100 +/- 49.6 to 106 +/- 58.6 mV, motility index from 548 +/- 296 to 567 +/- 377 V, n = 5800-6400 contractions). The diameter of the pyloric opening during the phase of relaxation was about 25% less in comparison to the control meal. The weaker antral contractions and the less opening of the pylorus diminished gastric evacuation and enhanced retropulsion of the gastric chyme. A higher incidence of segmental duodenal contractions produced a slower transfer of digesta. Results demonstrated that gastric emptying was regulated by several co-operating factors. The pylorus was involved by producing different resistances to flow during its phase of relaxation.
TL;DR: In this article, the separation of some anthocyanidins, proanthocyanin, and related substances by means of reversed-phase high-performance liquid chromatography is described.
TL;DR: The results suggest that, at least in the short term, the enhanced Fe(III) reduction by roots of Fe-deficient plants is not due to the secretion of reducing compounds.
Abstract: The hypothesized role of secreted reducing compounds in FeIII reduction has been examined with Fe-deficient peanuts (Arachis hypogaea L. cv A124B). Experiments involved the exposure of roots to (a) different gas mixtures, (b) carbonyl cyanide m-chlorophenylhydrazone (CCCP), and (c) agents which impair membrane integrity.
Removing roots from solution and exposing them to air or N2 for 10 minutes did not result in any accumulation in the free space of compounds capable of increasing rates of FeIII reduction when roots were returned to solutions. On the contrary, exposing roots to N2 decreased rates of FeIII reduction. CCCP also decreased rates of FeIII reduction.
Acetic acid and ethylenediaminetetraacetic acid (disodium salt) (EDTA) impaired the integrity and function of the plasma membranes of roots of Fe-deficient peanuts. That is, in the presence of acetic acid or EDTA, there was an efflux of K+ from the roots; K+ (86Rb) uptake was also impaired. Acetic acid increased the efflux from the roots of compounds capable of reducing FeIII. However, both acetic acid and EDTA caused rapid decreases in rates of FeIII reduction by the roots. In addition to peanuts, acetic acid also decreased rates of FeIII reduction by roots of Fe-deficient sunflowers (Helianthus annuus L. cv Sobrid) but not maize (Zea mays L. cv Garbo).
These results suggest that, at least in the short term, the enhanced FeIII reduction by roots of Fe-deficient plants is not due to the secretion of reducing compounds.
TL;DR: Inhibition of root elongation by high Zn concentrations was quite different in two ecotypes of Deschampsia caespitosa, suggesting that specific binding of Zn in the cytoplasm is a more likely mechanism for the detoxification of ZN in the apical meristems.
TL;DR: 2-Carboxy-4-(2-amino-3-bromophenyl)pyrrole (X) is demonstrated to be an intermediate in the biosynthesis of brominated pyrrolnitrin; the biosynthetic pathway to bromo derivatives of pyrolnitrin is discussed.
Abstract: The mutant strain ACN of Pseudoinonas aureofaciens ATCC 15926 produces several bromo derivatives of pyrrolnitrin. Five brominated amino- and three brominated nitrophenyl pyrrole compounds could be isolated, and their structures were established by 1H NMR, UV and mass spectroscopy. The isolated amino compounds showed no biological activity; the nitro derivatives inhibited the growth of Neurospora crassa ATCC 9276, though not as effective as pyrrolnitrin itself. 2-Carboxy-4-(2-amino-3-bromophenyl)pyrrole (X) is demonstrated to be an intermediate in the biosynthesis of brominated pyrrolnitrin; the biosynthetic pathway to bromo derivatives of pyrrolnitrin is discussed.
TL;DR: While its cytological adaptations indicate a nutritional dependence on the host, there is no evidence of ingestion of host-derived particulate material.
Abstract: . Trophonts of Pisdnoodinium pillulare (Schaperclaus, 1954), a common ectoparasite of freshwater aquarium fish, are attached to host cells by means of a specialized structure, the attachment disc. Unlike other dinoflagellate genera parasitic on fish and invertebrates, this disc features nail-like organelles, the rhizocysts. Head-parts of the rhizocysts are inverted in separate compartments, rhizothecas, in the sole of the disc while their long shafts are firmly embedded in the cytoplasm of cells of the host epidermis or gill epithelium. The attachment inflicts a serious injury on the host cells which may ultimately be destroyed. Rhizocysts originate in the subnuclear cytoplasm from where they migrate into the attachment disc. There are other specialized organelles and inclusions; fibrous vesicles, membraneous bodies, striated tubular bodies and paracrystalline bodies. Pisdnoodinium has well-developed chloroplasts. While its cytological adaptations indicate a nutritional dependence on the host, there is no evidence of ingestion of host-derived particulate material. Pisdnoodinium may derive an essential part of its nutrition from photosynthesis.
TL;DR: Dodecanoic acid was demonstrated to be linked with the hydroxy groups of the amide-bound fatty acids of a chloridazon-degrading bacterium, and the taxonomic significance of 2,3-diamino-2, 3-dideoxy-D-glucose, is discussed.
Abstract: Lipopolysaccharide of a chloridazon-degrading bacterium was obtained by a two-stage extraction procedure with phenol/EDTA in a yield of 0.3% of dried bacteria. The carbohydrate moiety consisted of heptose, 3-deoxyoctulosonic acid and D-glucose in a molar ratio of 1:2:2 X 3. Lipid A was composed of 1 mol 2,3-diamino-2,3-dideoxy-D-glucose, 2 mol amide-bound and 2.6 mol ester-bound fatty acids/mol. Amide-bound fatty acids were 3-hydroxydodecanoic acid and 3-hydroxyhexadecanoic acid; dodecanoic acid and R-(-)-3-hydroxydodec-5-cis-enoic acid were found to be present in ester linkage. Under conditions of acidic hydrolysis, the latter was converted into the cis and trans isomers of 5-hexyltetrahydrofuran-2-acetic acid. Dodecanoic acid was demonstrated to be linked with the hydroxy groups of the amide-bound fatty acids. The taxonomic significance of these results, especially the demonstration of 2,3-diamino-2, 3-dideoxy-D-glucose, is discussed.
TL;DR: The application of both chromatographic and mass spectrometric methods is demonstrated to be most valuable for the characterization of antibiotics with the unusual constituents α-aminoisobutyric acid, isovaline and phenylalaninol.
Abstract: The structural identity of the polypeptide antibiotics, samarosporin I(II) and stilbellin I(II) with emerimicin IV(III) has been established by thin-layer chromatography, quantitative amino acid analysis by ion-exchange chromatography, gas-liquid chromatography of the N-pentafluoropropionyl amino acid n-propyl esters and N,O-bis-pentafluoropropionyl phenylalaninol with quartz capillaries coated with the chiral stationary phase N-propionyl-L-valine-tert-butylamide, and determination of the relative molecular masses and sequence-specific fragments by field desorption fast atom bombardment mass spectrometry. The separation of closely related sequence analogues of the above polypeptides could be achieved by reversed-phase high-performance liquid chromatography with spherical, fully porous octadecylsilyl bonded phases, and 86% aqueous methanol as eluent. The application of both chromatographic and mass spectrometric methods is demonstrated to be most valuable for the characterization of antibiotics with the unusual constituents α-aminoisobutyric acid, isovaline and phenylalaninol. The methology employed is regarded to be applicable to all polypeptide antibiotics of the peptaibol class.
TL;DR: It was established that the common winter wheat cultivar Götz is of intermediate crossability, and interactions between wheats of different crossability classes and their seed set with rye lines were detected.
Abstract: Crossability and embryo development were studied in the crosses of one Triticum aestivum and three T. durum genotypes with nineteen rye inbred lines. Parental wheat and rye genotypes exerted a significant influence on the characters seed set, number of seeds containing embryos and viable plantlets obtained from embryo culture. It was established that the common winter wheat cultivar Gotz is of intermediate crossability. The rye inbred lines varied substantially in their capacity to fertilize several wheat genotypes. Interactions between wheats of different crossability classes and their seed set with rye lines were detected. Significant correlations were obtained between seed set and viable plantlets recovered in vitro.
TL;DR: The Co-methyl and the Co-aquo derivative of 5-hydroxybenzimidazolylcobamide (factor III) were isolated as the major natural corrinoids from Methanosarcina barkeri 'Fusaro' as mentioned in this paper.
TL;DR: In this article, the root surface area was estimated using a non-destructive root surface estimation method using methylenblau-methode and a methylene blue method.
Abstract: Um in Wasserkulturversuchen das Wurzelwachstum kontinuierlich verfolgen zu konnen, wurden zwei nicht-destruktive Methoden zur Bestimmung der Wurzeloberflache miteinander verglichen.
Bei der Methylenblau-Methode werden die Wurzeln 30 sec. mit dem kationischen Farbstoff Methylenblau angefarbt und der adsorbierte Farbstoff anschliesend mit Ca2+ (CaCl2-Losung) ausgetauscht. Die Menge an ausgetauschtem Methylenblau wird photometrisch bei 668 nm bestimmt. Bei der Tusche-Methode erfolgt das Anfarben der Wurzeln fur eine Minute mit einer verdunnten Losung (1:100) von Zeichentusche. Die an der Wurzeloberflache anhaftenden Tusche-Kolloide werden anschliesend mit destilliertem Wasser abgespult und deren Menge bei 500 nm photometrisch bestimmt. Die mit Hilfe dieser beiden Methoden geschatzten Wurzeloberflachen korrelieren hochsignifikant mit den aus Wurzelvolumen und Wurzellange errechneten Wurzeloberflachen.
Die mit der Tusche-Methode fur Kartoffelwurzeln gewonnene Eichgerade kann auch fur die Bestimmung der Oberflachen von Wurzeln anderer Pflanzenarten, z. B. Mais, verwendet werden. Dagegen mus bei der Methylenblau-Methode fur jede Pflanzenart ein von deren Kationenaustauschkapazitat abhangiger Faktor eingefuhrt werden. Bei dichtem Wurzelhaarbesatz kann es allerdings bei Verwendung der Tusche-Methode durch Tropfchenbildung in der Wurzelhaarzone zu Uberschatzungen der tatsachlichen Oberflache kommen. Fur kontinuierliche Untersuchungen der Wurzeloberflachen uber einen langeren Zeitraum ist die Tusche-Methode jedoch besser geeignet als die Methylenblau-Methode. Bei der Tusche-Methode traten auch bei haufiger Anwendung keine negativen Einflusse auf Wurzelwachstum oder Nahrstoffaufnahme auf.
Comparison of two non-destructive methods for determination of root surface areas
In order to study root growth and development continuously in water culture experiments, two non-destructive rapid methods of estimating root surface area were compared.
In the methylene blue method roots are stained for 30 seconds in methylene blue solution (74.7 mg/l). After washing off adhering methylene blue the adsorbed cationic dye is exchanged with Ca2+ by incubating the stained root in 0,1 M CaCl2 solution. The amount of exchangeable methylene blue is determined photometrically at 668 nm.
In the ink method, roots are stained for 1 minute with a 1:100 diluted solution of drawing ink. Excess ink is removed by carefully rotating the plant, and adhering ink is allowed to diffuse into destilled water. The quantity of ink is determined photometrically at 500 nm.
The root surface area of potato plants estimated with these two methods correlates highly significantly with the root surface area calculated from root volume and root length. In the case of the ink method the calibration curve obtained with potato roots may also be used for other plant species like maize. This is not possible with the methylene blue method. A correction factor depending on the cation exchange capacity of the roots would be needed for this method. With the ink method overestimation of root surface area may occur if abundant root hairs are present. This is due to the formation of droplets in the root hair zone. However for repeated measurements during a growth period the ink method is more suitable since with this method no negative effect on root growth and nutrient uptake can be observed.
TL;DR: In this article, the authors investigated the effect of high nitrate application on the kinetic and gaseous composition of denitrification in 16 soil horizons after application of a relatively high amount of nitrate (400 μg NO N/g dry soil) at defined conditions (He atmosphere; 80 % WHC; 30°C; 3 weeks).
Abstract: In Modellversuchen wurde die Kinetik und Zusammensetzung (Quantitat und Qualitat) der Denitrifikation von 16 verschiedenen Horizonten unter definierten Bedingungen (He-Atmosphare; 80 % mWK; 30°C; 3 Wochen) nach einer relativ hohen Nitratbelastung (400 μg NON/g TB) verfolgt. Unter den gestellten Bedingungen entsprach die Stickstoff-Entgasung in allen Proben einer Funktion nullter Ordnung. Die Denitrifikationskapazitat war ausschlieslich abhangig vom Gehalt an leichtmineralisierbarem Kohlenstoff (gemessen als CH2O oder Cmin). N2 und N2O wurden stets entbunden; NO konnte nicht nachgewiesen werden. Zum Versuchsende war das N2/N-N2O-Verhaltnis stets groser als 1 (im Schnitt 3.1/1), ausgenommen beim sauren Ah-Material der Pararendzina (N2/N-N2O = 1.0/1.2). Durch Inkorporation eines Uberangebots an leichtmineralisierbarem organischen Material (Laubpulver) wurde eine vollstandige Denitrifikation bis zum N2 erreicht. Lediglich im sauren „B”-Material der Pararendzina wurde N2O nachgewiesen. Die Denitrifikationskapazitat eines Bodens (Y in μg N2+N-N2O/g TB) last sich unter den gestellten Voraussetzungen aus der Gleichung Y = 0.808 X + 120,1 vorausschatzen (X in μg CH2O/g TB).
Effect of high nitrate application on the kinetic and gaseous composition of denitrification in different soils
Kinetic and gaseous composition of denitrification were studied with 16 different soil horizons after application of a relatively high amount of nitrate (400 μg NO N/g dry soil) at defined conditions (He atmosphere; 80 % WHC; 30°C; 3 weeks). At the conditions given, denitrification followed zero-order kinetics depending on the amount of decomposable organic matter rather on than the nitrate concentration. Denitrification intensity was most significantly correlated with the amount of extractable or mineralizable organic carbon (CH2O and Cmin, respectively). N2 and N2O (but not NO) were liberated by all samples, the amount and ratio depending on the soil and time of sampling. At the end of the three week's incubation period, the N2/N-N2O ratio varied between 8.1 and 1.4/1 (with an average of 3.1/1), except for the acid Ah-sample of the Pararendzina (N2/N-N2O = 1.0/1.2). The incorporation of an excess of easily decomposable leaf-powder resulted in an increased nitrate turn-over (from 64.7 % to 95,7 % of the nitrate loss) with N2 as the single denitrification product. N2O could be detected only with the acid “B”-material of the Pararendzina. The denitrifying capacity Y (in μg N2+N-N2O/g soil) of a soil at the conditions given may be predicted by the amount of water-extractable organic carbon X (in μg CH2O/g soil) according to the equation Y = 0.808 X + 120,1.
TL;DR: Three groups of male, castrated piglets of the German Landrace breed were used to study the relation between fattening performance and blood parameters when feeding rations containing different amounts of fat and a significant positive relation between body weight and digestibility of the fat was determined.
Abstract: Three groups of male, castrated piglets of the German Landrace breed, weight range 3-30 kg, were used to study the relation between fattening performance and blood parameters when feeding rations containing different amounts of fat. The fat content of the rations was either 5% (group I), 18% (group II) or 35% (group III). Concomitantly with the increased metabolizable energy (ME) content of the ration the content of digestible protein (DP) was increased and the amount of feed reduced in order to guarantee an equal intake of ME and DP in all three groups. The digestibility of the crude nutrients and protein retention of the subjects were determined in nine subsequent trials each lasting 7 days. At a body weight of 27 kg the blood concentrations of insulin, glucose, free fatty acids, neutral lipids and cholesterol were determined at different times after feeding. In addition, an oral glucose tolerance test was made and the in-vitro synthesis of fat from glucose was measured. The apparent digestibility of fat amounted, unexpectedly, to approximately 94% on the high rat rations II and III. In all groups a significant positive relation between body weight and digestibility of the fat was determined. Despite equal daily intakes of ME in all groups, in group III daily weight gain and protein retention were 7% (p less than 0.01) and 4% (p less than 0.01) higher than in group I, respectively. Blood urea levels of group III were 67% (p less than 0.01) lower than in group I. The mean daily nitrogen retention of the three groups rose gradually from 4 g at a body weight of 5 kg to 16-17 g at a body weight of 25 kg. The feed conversion of group I was lower by 22 and 36% as compared to groups II and III, respectively. Highly significant differences were observed among either groups (p less than 0.01). It has been calculated that in group III the consumption of ME/kg weight gain was about 8% (p less than 0.05) lower than in the two other groups. According to the increased fat percentage of the rations, body protein content diminished from 17,1% in group I to 16,5 and 16% in groups II and III, respectively. Only the difference between groups I and III proved to be significant (p less than 0.05). In group I feed intake resulted in an increase in the insulin level by 55% (p less than 0.05) whereas glucose did not change significantly.(ABSTRACT TRUNCATED AT 400 WORDS)
TL;DR: In this paper, the authors found that methylmercuric chloride (MeHg) is extremely toxic to the freshwater flagellate Poterioochromonas malhamensis, leading to the formation of giant multinucleared cells, polyploid nuclei and numerous alterations in the nuclear and the nucleolar structures.