Institution
University of Hohenheim
Education•Stuttgart, Germany•
About: University of Hohenheim is a education organization based out in Stuttgart, Germany. It is known for research contribution in the topics: Population & Soil water. The organization has 8585 authors who have published 16406 publications receiving 567377 citations.
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TL;DR: It is demonstrated that an intron of the ey gene contains an enhancer that regulates the eye specific expression of the gene in the eye disc primordia of embryos and in the eyes imaginal discs of third instar larvae, and proposed a Pax-6-like molecule to be a positive transactivator for the eyespecific ey expression.
Abstract: The development of the Drosophila compound eye requires the function of a set of evolutionarily conserved genes. Among these, the Drosophila Pax-6 gene eyeless (ey) plays a major role. ey has been considered a master control gene of eye development in the animal kingdom because targeted expression of ey and vertebrate as well as invertebrate homologs lead to the formation of ectopic eyes in Drosophila. We demonstrate that an intron of the ey gene contains an enhancer that regulates the eye specific expression of the gene in the eye disc primordia of embryos and in the eye imaginal discs of third instar larvae. Moreover, a 212-bp enhancer element is necessary and sufficient for the enhancer function. It is partially conserved in Drosophila hydei and contains putative Pax-6 Paired domain binding sites. We show that several binding sites are required for the eye specific expression, and, therefore, we propose a Pax-6-like molecule to be a positive transactivator for the eye specific ey expression. This transactivator recently has been identified as twin of eyeless, the second Pax-6 gene in Drosophila.
139 citations
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TL;DR: The present study identified eight key enzymes by mass spectrometry and partially by activity staining on native polyacrylamide gels or gel zymography and purified Flavourzyme enzymes were biochemically characterized with regard to pH and temperature profiles and molecular sizes.
Abstract: Flavourzyme is sold as a peptidase preparation from Aspergillus oryzae. The enzyme preparation is widely and diversely used for protein hydrolysis in industrial and research applications. However, detailed information about the composition of this mixture is still missing due to the complexity. The present study identified eight key enzymes by mass spectrometry and partially by activity staining on native polyacrylamide gels or gel zymography. The eight enzymes identified were two aminopeptidases, two dipeptidyl peptidases, three endopeptidases, and one α-amylase from the A. oryzae strain ATCC 42149/RIB 40 (yellow koji mold). Various specific marker substrates for these Flavourzyme enzymes were ascertained. An automated, time-saving nine-step protocol for the purification of all eight enzymes within 7 h was designed. Finally, the purified Flavourzyme enzymes were biochemically characterized with regard to pH and temperature profiles and molecular sizes.
139 citations
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TL;DR: In this paper, the differences between social interactions in online and offline contexts as well as users' satisfaction with the social support received in these contexts were investigated, and it was hypothesized that SNSs are better set up for the task of leveraging informational support but that they are inferior to offline contexts in terms of emotional or instrumental support.
Abstract: People around the globe now regularly interact with family and friends through social network sites (SNSs). In this article, we investigated the differences between social interactions in online and offline contexts as well as users' satisfaction with the social support received in these contexts. It was hypothesized that SNSs are better set up for the task of leveraging informational support but that they are inferior to offline contexts in terms of emotional or instrumental support. We further assumed that users might feel similarly satisfied with how support is rendered online and offline but that only social support transacted in offline contexts would contribute to overall life satisfaction. All hypotheses were supported by longitudinal data (N = 327) that were used to investigate social support over the course of 2 years with 4 points of measurement.
139 citations
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TL;DR: In this paper, two different digestates were used as test fuel and pressed into pellets to verify whether digestate from biogas plants is suitable as a solid biomass fuel and to classify the digestate according to current regulations for biofuels.
139 citations
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TL;DR: Coco acts as a critical target of flow, suggesting that symmetry is broken by flow-mediated left-asymmetric release of Nodal repression at the midline.
139 citations
Authors
Showing all 8665 results
Name | H-index | Papers | Citations |
---|---|---|---|
Robert J. Lefkowitz | 214 | 860 | 147995 |
Patrick O. Brown | 183 | 755 | 200985 |
Mark Stitt | 132 | 456 | 60800 |
Wolf B. Frommer | 105 | 345 | 30918 |
Muhammad Imran | 94 | 3053 | 51728 |
Muhammad Farooq | 92 | 1341 | 37533 |
Yakov Kuzyakov | 87 | 667 | 37050 |
Werner Goebel | 85 | 367 | 26106 |
Ismail Cakmak | 84 | 249 | 25991 |
Reinhold Carle | 84 | 418 | 24858 |
Michael Wink | 83 | 938 | 32658 |
Albrecht E. Melchinger | 83 | 398 | 23140 |
Tilman Grune | 82 | 479 | 30327 |
Volker Römheld | 79 | 231 | 20763 |
Klaus Becker | 79 | 320 | 27494 |