Showing papers by "University of Illinois at Urbana–Champaign published in 2000"

Data Mining: Concepts and Techniques

Abstract: The increasing volume of data in modern business and science calls for more complex and sophisticated tools. Although advances in data mining technology have made extensive data collection much easier, it's still always evolving and there is a constant need for new techniques and tools that can help us transform this data into useful information and knowledge. Since the previous edition's publication, great advances have been made in the field of data mining. Not only does the third of edition of Data Mining: Concepts and Techniques continue the tradition of equipping you with an understanding and application of the theory and practice of discovering patterns hidden in large data sets, it also focuses on new, important topics in the field: data warehouses and data cube technology, mining stream, mining social networks, and mining spatial, multimedia and other complex data. Each chapter is a stand-alone guide to a critical topic, presenting proven algorithms and sound implementations ready to be used directly or with strategic modification against live data. This is the resource you need if you want to apply today's most powerful data mining techniques to meet real business challenges. * Presents dozens of algorithms and implementation examples, all in pseudo-code and suitable for use in real-world, large-scale data mining projects. * Addresses advanced topics such as mining object-relational databases, spatial databases, multimedia databases, time-series databases, text databases, the World Wide Web, and applications in several fields. *Provides a comprehensive, practical look at the concepts and techniques you need to get the most out of real business data

The capacity of wireless networks

Abstract: When n identical randomly located nodes, each capable of transmitting at W bits per second and using a fixed range, form a wireless network, the throughput /spl lambda/(n) obtainable by each node for a randomly chosen destination is /spl Theta/(W//spl radic/(nlogn)) bits per second under a noninterference protocol. If the nodes are optimally placed in a disk of unit area, traffic patterns are optimally assigned, and each transmission's range is optimally chosen, the bit-distance product that can be transported by the network per second is /spl Theta/(W/spl radic/An) bit-meters per second. Thus even under optimal circumstances, the throughput is only /spl Theta/(W//spl radic/n) bits per second for each node for a destination nonvanishingly far away. Similar results also hold under an alternate physical model where a required signal-to-interference ratio is specified for successful receptions. Fundamentally, it is the need for every node all over the domain to share whatever portion of the channel it is utilizing with nodes in its local neighborhood that is the reason for the constriction in capacity. Splitting the channel into several subchannels does not change any of the results. Some implications may be worth considering by designers. Since the throughput furnished to each user diminishes to zero as the number of users is increased, perhaps networks connecting smaller numbers of users, or featuring connections mostly with nearby neighbors, may be more likely to be find acceptance.

Subjective well-being. The science of happiness and a proposal for a national index.

Abstract: One area of positive psychology analyzes subjective well-being (SWB), people's cognitive and affective evaluations of their lives. Progress has been made in understanding the components of SWB, the importance of adaptation and goals to feelings of well-being, the temperament underpinnings of SWB, and the cultural influences on well-being. Representative selection of respondents, naturalistic experience sampling measures, and other methodological refinements are now used to study SWB and could be used to produce national indicators of happiness.

A model for types and levels of human interaction with automation

Abstract: We outline a model for types and levels of automation that provides a framework and an objective basis for deciding which system functions should be automated and to what extent. Appropriate selection is important because automation does not merely supplant but changes human activity and can impose new coordination demands on the human operator. We propose that automation can be applied to four broad classes of functions: 1) information acquisition; 2) information analysis; 3) decision and action selection; and 4) action implementation. Within each of these types, automation can be applied across a continuum of levels from low to high, i.e., from fully manual to fully automatic. A particular system can involve automation of all four types at different levels. The human performance consequences of particular types and levels of automation constitute primary evaluative criteria for automation design using our model. Secondary evaluative criteria include automation reliability and the costs of decision/action consequences, among others. Examples of recommended types and levels of automation are provided to illustrate the application of the model to automation design.

The rank-order consistency of personality traits from childhood to old age: a quantitative review of longitudinal studies.

Abstract: The present study used meta-analytic techniques to test whether trait consistency maximizes and stabilizes at a specific period in the life course. From 152 longitudinal studies, 3,217 test-retest correlation coefficients were compiled. Meta-analytic estimates of mean population test-retest correlation coefficients showed that trait consistency increased from .31 in childhood to .54 during the college years, to .64 at age 30, and then reached a plateau around .74 between ages 50 and 70 when time interval was held constant at 6.7 years. Analysis of moderators of consistency showed that the longitudinal time interval had a negative relation to trait consistency and that temperament dimensions were less consistent than adult personality traits.

Selection on Observed and Unobserved Variables: Assessing the Effectiveness of Catholic Schools

Abstract: We develop estimation methods that use the amount of selection on the observables in a model as a guide to the amount of selection on the unobservables. We show that if the observed variables are a random subset of a large number of factors that influence the endogenous variable and the outcome of interest, then the relationship between the index of observables that determines the endogenous variable and the index that determines the outcome will be the same as the relationship between the indices of unobservables that determine the two variables. In some circumstances this fact may be used to identify the effect of the endogenous variable. We also propose an informal way to assess selectivity bias based on measuring the ratio of selection on unobservables to selection on observables that would be required if one is to attribute the entire effect of the endogenous variable to selection bias. We use our methods to estimate the effect of attending a Catholic high school on a variety of outcomes. Our main conclusion is that Catholic high schools substantially increase the probability of graduating from high school and, more tentatively, college attendance. We do not find much evidence for an effect on test scores.

Toward a psychology of positive youth development

Abstract: This article analyzes the development of initiative as an exemplar of one of many learning experiences that should be studied as part of positive youth development. The capacity for initiative is essential for adults in our society and will become more important in the 21st century, yet adolescents have few opportunities to learn it. Their typical experiences during schoolwork and unstructured leisure do not reflect conditions for learning initiative. The context best suited to the development of initiative appears to be that of structured voluntary activities, such as sports, arts, and participation in organizations, in which youths experience the rare combination of intrinsic motivation in combination with deep attention. An incomplete body of outcome research suggests that such activities are associated with positive development, but the developmental processes involved are only beginning to be understood. One promising approach has recorded language use and has found that adolescents participating in effective organizations acquire a new operating language that appears to correspond to the development of initiative.

Brain-computer interface technology: a review of the first international meeting

Abstract: Over the past decade, many laboratories have begun to explore brain-computer interface (BCI) technology as a radically new communication option for those with neuromuscular impairments that prevent them from using conventional augmentative communication methods. BCI's provide these users with communication channels that do not depend on peripheral nerves and muscles. This article summarizes the first international meeting devoted to BCI research and development. Current BCI's use electroencephalographic (EEG) activity recorded at the scalp or single-unit activity recorded from within cortex to control cursor movement, select letters or icons, or operate a neuroprosthesis. The central element in each BCI is a translation algorithm that converts electrophysiological input from the user into output that controls external devices. BCI operation depends on effective interaction between two adaptive controllers, the user who encodes his or her commands in the electrophysiological input provided to the BCI, and the BCI which recognizes the commands contained in the input and expresses them in device control. Current BCI's have maximum information transfer rates of 5-25 b/min. Achievement of greater speed and accuracy depends on improvements in signal processing, translation algorithms, and user training. These improvements depend on increased interdisciplinary cooperation between neuroscientists, engineers, computer programmers, psychologists, and rehabilitation specialists, and on adoption and widespread application of objective methods for evaluating alternative methods. The practical use of BCI technology depends on the development of appropriate applications, identification of appropriate user groups, and careful attention to the needs and desires of individual users. BCI research and development will also benefit from greater emphasis on peer-reviewed publications, and from adoption of standard venues for presentations and discussion.

Observational evidence of recent change in the northern high-latitude environment

Abstract: Studies from a variety of disciplines documentrecentchange in the northern high-latitude environment.Prompted by predictions of an amplified response oftheArctic to enhanced greenhouse forcing, we present asynthesis of these observations. Pronounced winter andspring warming over northern continents since about 1970ispartly compensated by cooling over the northern NorthAtlantic. Warming is also evident over the centralArcticOcean. There is a downward tendency in sea ice extent,attended by warming and increased areal extent of theArctic Ocean's Atlantic layer. Negative snow coveranomalies have dominated over both continents sincethelate 1980s and terrestrial precipitation has increasedsince 1900. Small Arctic glaciers have exhibitedgenerally negative mass balances. While permafrost haswarmed in Alaska and Russia, it has cooled in easternCanada. There is evidence of increased plant growth,attended by greater shrub abundance and northwardmigration of the tree line. Evidence also suggeststhatthe tundra has changed from a net sink to a net sourceofatmospheric carbon dioxide.Taken together, these results paint a reasonablycoherent picture of change, but their interpretationassignals of enhanced greenhouse warming is open todebate.Many of the environmental records are either short,areof uncertain quality, or provide limited spatialcoverage. The recent high-latitude warming is also nolarger than the interdecadal temperature range duringthis century. Nevertheless, the general patterns ofchange broadly agree with model predictions. Roughlyhalfof the pronounced recent rise in Northern Hemispherewinter temperatures reflects shifts in atmosphericcirculation. However, such changes are notinconsistentwith anthropogenic forcing and include generallypositive phases of the North Atlantic and ArcticOscillations and extratropical responses to theEl-NinoSouthern Oscillation. An anthropogenic effect is alsosuggested from interpretation of the paleoclimaterecord,which indicates that the 20th century Arctic is thewarmest of the past 400 years.

Guidelines for using human event-related potentials to study cognition: Recording standards and publication criteria

Abstract: Event-related potentials ~ERPs! recorded from the human scalp can provide important information about how the human brain normally processes information and about how this processing may go awry in neurological or psychiatric disorders. Scientists using or studying ERPs must strive to overcome the many technical problems that can occur in the recording and analysis of these potentials. The methods and the results of these ERP studies must be published in a way that allows other scientists to understand exactly what was done so that they can, if necessary, replicate the experiments. The data must then be analyzed and presented in a way that allows different studies to be compared readily. This paper presents guidelines for recording ERPs and criteria for publishing the results.

Functional hydrogel structures for autonomous flow control inside microfluidic channels

Abstract: Hydrogels have been developed to respond to a wide variety of stimuli, but their use in macroscopic systems has been hindered by slow response times (diffusion being the rate-limiting factor governing the swelling process) However, there are many natural examples of chemically driven actuation that rely on short diffusion paths to produce a rapid response It is therefore expected that scaling down hydrogel objects to the micrometre scale should greatly improve response times At these scales, stimuli-responsive hydrogels could enhance the capabilities of microfluidic systems by allowing self-regulated flow control Here we report the fabrication of active hydrogel components inside microchannels via direct photopatterning of a liquid phase Our approach greatly simplifies system construction and assembly as the functional components are fabricated in situ, and the stimuli-responsive hydrogel components perform both sensing and actuation functions We demonstrate significantly improved response times (less than 10 seconds) in hydrogel valves capable of autonomous control of local flow

Vortex organization in the outer region of the turbulent boundary layer

Abstract: The structure of energy-containing turbulence in the outer region of a zero-pressure- gradient boundary layer has been studied using particle image velocimetry (PIV) to measure the instantaneous velocity fields in a streamwise-wall-normal plane. Experiments performed at three Reynolds numbers in the range 930 0) that occur on a locus inclined at 30–60° to the wall.In the outer layer, hairpin vortices occur in streamwise-aligned packets that propagate with small velocity dispersion. Packets that begin in or slightly above the buffer layer are very similar to the packets created by the autogeneration mechanism (Zhou, Adrian & Balachandar 1996). Individual packets grow upwards in the streamwise direction at a mean angle of approximately 12°, and the hairpins in packets are typically spaced several hundred viscous lengthscales apart in the streamwise direction. Within the interior of the envelope the spatial coherence between the velocity fields induced by the individual vortices leads to strongly retarded streamwise momentum, explaining the zones of uniform momentum observed by Meinhart & Adrian (1995). The packets are an important type of organized structure in the wall layer in which relatively small structural units in the form of three-dimensional vortical structures are arranged coherently, i.e. with correlated spatial relationships, to form much longer structures. The formation of packets explains the occurrence of multiple VITA events in turbulent ‘bursts’, and the creation of Townsend's (1958) large-scale inactive motions. These packets share many features of the hairpin models proposed by Smith (1984) and co-workers for the near-wall layer, and by Bandyopadhyay (1980), but they are shown to occur in a hierarchy of scales across most of the boundary layer.In the logarithmic layer, the coherent vortex packets that originate close to the wall frequently occur within larger, faster moving zones of uniform momentum, which may extend up to the middle of the boundary layer. These larger zones are the induced interior flow of older packets of coherent hairpin vortices that originate upstream and over-run the younger, more recently generated packets. The occurence of small hairpin packets in the environment of larger hairpin packets is a prominent feature of the logarithmic layer. With increasing Reynolds number, the number of hairpins in a packet increases.

Observed Variability and Trends in Extreme Climate Events: A Brief Review

Abstract: Variations and trends in extreme climate events have only recently received much attention. Exponentially increasing economic losses, coupled with an increase in deaths due to these events, have focused attention on the possibility that these events are increasing in frequency. One of the major problems in examining the climate record for changes in extremes is a lack of high-quality, long-term data. In some areas of the world increases in extreme events are apparent, while in others there appears to be a decline. Based on this information increased ability to monitor and detect multidecadal variations and trends is critical to begin to detect any observed changes and understand their origins.

A colorimetric sensor array for odour visualization

Abstract: Array-based vapour-sensing devices are used to detect and differentiate between chemically diverse analytes. These systems--based on cross-responsive sensor elements--aim to mimic the mammalian olfactory system by producing composite responses unique to each odorant. Previous work has concentrated on a variety of non-specific chemical interactions to detect non-coordinating organic vapours. But the most odiferous, toxic compounds often bind readily to metal ions. Here we report a simple optical chemical sensing method that utilizes the colour change induced in an array of metalloporphyrin dyes upon ligand binding while minimizing the need for extensive signal transduction hardware. The chemoselective response of a library of immobilized vapour-sensing metalloporphyrin dyes permits the visual identification of a wide range of ligating (alcohols, amines, ethers, phosphines, phosphites, thioethers and thiols) and even weakly ligating (arenes, halocarbons and ketones) vapours. Water vapour does not affect the performance of the device, which shows a good linear response to single analytes, and interpretable responses to analyte mixtures. Unique colour fingerprints can be obtained at analyte concentrations below 2 parts per million, and responses to below 100 parts per billion have been observed. We expect that this type of sensing array will be of practical importance for general-purpose vapour dosimeters and analyte-specific detectors (for insecticides, drugs or neurotoxins, for example).

The mental prosthesis: assessing the speed of a P300-based brain-computer interface

Abstract: Describes a study designed to assess a brain-computer interface (BCI), originally described by Farwell and Donchin in 1988. The system utilizes the fact that the rare events in the oddball paradigm elicit the P300 component of the event-related potential (ERP). The BCI presents the user with a matrix of 6 by 6 cells, each containing one letter of the alphabet. The user focuses attention on the cell containing the letter to be communicated while the rows and the columns of the matrix are intensified. Each intensification is an event in the oddball sequence, the row and the column containing the attended cell are "rare" items and, therefore, only these events elicit a P300. The computer thus detects the transmitted character by determining which row and which column elicited the P300. The authors report an assessment, using a bootstrapping approach, which indicates that an off line version of the system can communicate at the rate of 7.8 characters a minute and achieve 80% accuracy. The system's performance in real time was also assessed. The authors' data indicate that a P300-based BCI is feasible and practical. However, these conclusions are based on tests using healthy individuals.

Phase noise in oscillators: a unifying theory and numerical methods for characterization

Abstract: Phase noise is a topic of theoretical and practical interest in electronic circuits, as well as in other fields, such as optics. Although progress has been made in understanding the phenomenon, there still remain significant gaps, both in its fundamental theory and in numerical techniques for its characterization. In this paper, we develop a solid foundation for phase noise that is valid for any oscillator, regardless of operating mechanism. We establish novel results about the dynamics of stable nonlinear oscillators in the presence of perturbations, both deterministic and random. We obtain an exact nonlinear equation for phase error, which we solve without approximations for random perturbations. This leads us to a precise characterization of timing jitter and spectral dispersion, for computing of which we have developed efficient numerical methods. We demonstrate our techniques on a variety of practical electrical oscillators and obtain good matches with measurements, even at frequencies close to the carrier, where previous techniques break down. Our methods are more than three orders of magnitude faster than the brute-force Monte Carlo approach, which is the only previously available technique that can predict phase noise correctly.

Passive mixing in a three-dimensional serpentine microchannel

Abstract: A three-dimensional serpentine microchannel design with a "C shaped" repeating unit is presented in this paper as a means of implementing chaotic advection to passively enhance fluid mixing. The device is fabricated in a silicon wafer using a double-sided KOH wet-etching technique to realize a three-dimensional channel geometry. Experiments using phenolphthalein and sodium hydroxide solutions demonstrate the ability of flow in this channel to mix faster and more uniformly than either pure molecular diffusion or flow in a "square-wave" channel for Reynolds numbers from 6 to 70. The mixing capability of the channel increases with increasing Reynolds number. At least 98% of the maximum intensity of reacted phenolphthalein is observed in the channel after five mixing segments for Reynolds numbers greater than 25. At a Reynolds number of 70, the serpentine channel produces 16 times more reacted phenolphthalein than a straight channel and 1.6 times more than the square-wave channel. Mixing rates in the serpentine channel at the higher Reynolds numbers are consistent with the occurrence of chaotic advection. Visualization of the interface formed in the channel between streams of water and ethyl alcohol indicates that the mixing is due to both diffusion and fluid stirring.

The Catalytic Pathway of Cytochrome P450Cam at Atomic Resolution

Abstract: Members of the cytochrome P450 superfamily catalyze the addition of molecular oxygen to nonactivated hydrocarbons at physiological temperature-a reaction that requires high temperature to proceed in the absence of a catalyst. Structures were obtained for three intermediates in the hydroxylation reaction of camphor by P450cam with trapping techniques and cryocrystallography. The structure of the ferrous dioxygen adduct of P450cam was determined with 0.91 angstrom wavelength x-rays; irradiation with 1.5 angstrom x-rays results in breakdown of the dioxygen molecule to an intermediate that would be consistent with an oxyferryl species. The structures show conformational changes in several important residues and reveal a network of bound water molecules that may provide the protons needed for the reaction.

Three-dimensional micro-channel fabrication in polydimethylsiloxane (PDMS) elastomer

Abstract: This paper describes a fabrication technique for building three-dimensional (3-D) micro-channels in polydimethylsiloxane (PDMS) elastomer. The process allows for the stacking of many thin (less than 100-/spl mu/m thick) patterned PDMS layers to realize complex 3-D channel paths. The master for each layer is formed on a silicon wafer using an epoxy-based photoresist (SU 8). PDMS is cast against the master producing molded layers containing channels and openings. To realize thin layers with openings, a sandwich molding configuration was developed that allows precise control of the PDMS thickness. The master wafer is clamped within a sandwich that includes flat aluminum plates, a flexible polyester film layer, a rigid Pyrex wafer, and a rubber sheet. A parametric study is performed on PDMS surface activation in a reactive-ion-etching system and the subsequent methanol treatment for bonding and aligning very thin individual components to a substrate. Low RF power and short treatment times are better than high RF power and long treatment times, respectively, for instant bonding. Layer-to-layer alignment of less then 15 /spl mu/m is achieved with manual alignment techniques that utilize surface tension driven self-alignment methods. A coring procedure is used to realize off-chip fluidic connections via the bottom PDMS layer, allowing the top layer to remain smooth and flat for complete optical access.

Urban Sprawl: Diagnosis and Remedies:

Abstract: This article argues that urban spatial expansion results mainly from three powerful forces: a growing population, rising incomes, and falling commuting costs. Urban growth occurring purely in response to these fundamental forces cannot be faulted as socially undesirable, but three market failures may distort their operation, upsetting the allocation of land between agricultural and urban uses and justifying criticism of urban sprawl. These are the failure to account for the benefits of open space, excessive commuting because of a failure to account for the social costs of congestion, and failure to make new development pay for the infrastructure costs it generates. Precise remedies for these market failures are two types of development taxes and congestion tolls levied on commuters. Each of these remedies leads to a reduction in the spatial size of the city.

Increased adipose tissue in male and female estrogen receptor-α knockout mice

Abstract: Estrogen regulates the amount of white adipose tissue (WAT) in females, but its role in males and whether WAT effects involve estrogen receptor-α (ERα) or ERβ were unclear. We analyzed the role of ERα in WAT and brown adipose tissue by comparing these tissues in wild-type (WT) and ERα-knockout (αERKO) male and female mice. Brown adipose tissue weight was similar in αERKO and WT males at all ages. Progressive increases in WAT were seen in αERKO males with advancing age. Epididymal, perirenal, and inguinal WAT weighed 139–185% more in αERKO than in WT males by 270–360 days of age. Epididymal and perirenal adipocyte size was increased 20% in αERKO males. Adipocyte number was 82–168% greater in fat pads of αERKO vs. WT males. Compared with WT, 90-day-old αERKO females had increases in fat pad weights (54–103%), adipocyte size, and number. Both αERKO males and females had insulin resistance and impaired glucose tolerance, similar to humans lacking ERα or aromatase. Energy intake was equal in WT and αERKO males, indicating that obesity was not induced by hyperphagia. In contrast, energy expenditure was reduced by 11% in αERKO compared with WT males, indicating that altered energy expenditure may be important for the observed obesity. In summary, ERα absence causes adipocyte hyperplasia and hypertrophy, insulin resistance, and glucose intolerance in both sexes. These results are evidence that estrogen/ERα signaling is critical in female and male WAT; obesity in αERKO males involves a mechanism of reduced energy expenditure rather than increased energy intake.

A benefit congruency framework of sales promotion effectiveness

Abstract: Are monetary savings the only explanation for consumer response to a sales promotion? If not, how do the different consumer benefits of a sales promotion influence its effectiveness? To address the first question, this research builds a framework of the multiple consumer benefits of a sales promotion. Through a series of measurement studies, the authors find that monetary and nonmonetary promotions provide consumers with different levels of three hedonic benefits (opportunities for value expression, entertainment, and exploration) and three utilitarian benefits (savings, higher product quality, and improved shopping convenience). To address the second question, the authors develop a benefit congruency framework, which argues that a sales promotion’s effectiveness is determined by the utilitarian or hedonic nature of the benefits it delivers and the congruence these benefits have with the promoted product. Among other results, two choice experiments show that, as predicted for high-equity brands, ...

Classifying Managerial Responses to Multiple Organizational Identities

Abstract: We examine the phenomenon of multiple organizational identities and suggest that they can be managed in organizations by changing the number of (identity plurality) or relationships among (identity synergy) the identities. Using “plurality” and “synergy” as response dimensions, we offer a classification scheme identifying four major types of managerial responses: compartmentalization, deletion, integration, and aggregation. We also suggest several key conditions that may affect the use and appropriateness of these identity management responses and develop a series of propositions for research.

A Survey of Parallel Genetic Algorithms

Abstract: Genetic algorithms (GAs) are powerful search techniques that are used successfully to solve problems in many different disciplines. Parallel GAs are particularly easy to implement and promise substantial gains in performance. As such, there has been extensive research in this field. This survey attempts to collect, organize, and present in a unified way some of the most representative publications on parallel genetic algorithms. To organize the literature, the paper presents a categorization of the techniques used to parallelize GAs, and shows examples of all of them. However, since the majority of the research in this field has concentrated on parallel GAs with multiple populations, the survey focuses on this type of algorithms. Also, the paper describes some of the most significant problems in modeling and designing multi-population parallel GAs and presents some recent advancements.

The renaissance of fluorescence resonance energy transfer

Abstract: Recent advances in fluorescence resonance energy transfer have led to qualitative and quantitative improvements in the technique, including increased spatial resolution, distance range, and sensitivity These advances, due largely to new fluorescent dyes, but also to new optical methods and instrumentation, have opened up new biological applications

The Good, the Bad, and the Ambivalent: Managing Identification among Amway Distributors

Abstract: An ethnographic study of distributors for Amway, a network marketing organization, examines the practices and processes involved in managing members' organizational identification. It shows that this organization manages identification by using two types of practices: sensebreaking practices that break down meaning and sensegiving practices that provide meaning. When both sensebreaking and sensegiving practices are successful, members positively identify with the organization. When either sensebreaking or sensegiving practices fail, members deidentify, disidentify, or experience ambivalent identification with the organization. A general model of identification management is posited, and implications for both theory and practice are offered.

Speeding molecular recognition by using the folding funnel: The fly-casting mechanism

Abstract: Protein folding and binding are kindred processes. Many proteins in the cell are unfolded, so folding and function are coupled. This paper investigates how binding kinetics is influenced by the folding of a protein. We find that a relatively unstructured protein molecule can have a greater capture radius for a specific binding site than the folded state with its restricted conformational freedom. In this scenario of binding, the unfolded state binds weakly at a relatively large distance followed by folding as the protein approaches the binding site: the “fly-casting mechanism.” We illustrate this scenario with the hypothetical kinetics of binding a single repressor molecule to a DNA site and find that the binding rate can be significantly enhanced over the rate of binding of a fully folded protein.