University of Johannesburg

About: University of Johannesburg is a education organization based out in Johannesburg, South Africa. It is known for research contribution in the topics: Population & Tourism. The organization has 8070 authors who have published 22749 publications receiving 329408 citations. The organization is also known as: UJ.

Physical conditions of the interstellar medium of high-redshift, strongly lensed submillimetre galaxies from the Herschel-ATLAS

Abstract: We present Herschel-Spectral and Photometric Imaging Receiver (SPIRE) Fourier transform spectrometer (FTS) and radio follow-up observations of two Herschel-Astrophysical Terahertz Large Area Survey (H-ATLAS)-detected strongly lensed distant galaxies. In one of the targeted galaxies H-ATLAS J090311.6+003906 (SDP.81), we detect [O iii] 88 μm and [C ii] 158 μm lines at a signal-to-noise ratio of ∼5. We do not have any positive line identification in the other fainter target H-ATLAS J091305.0−005343 (SDP.130). Currently, SDP.81 is the faintest submillimetre galaxy with positive line detections with the FTS, with continuum flux just below 200 mJy in the 200–600 μm wavelength range. The derived redshift of SDP.81 from the two detections is z = 3.043 ± 0.012, in agreement with ground-based CO measurements. This is the first detection by Herschel of the [O iii] 88 μm line in a galaxy at redshift higher than 0.05. Comparing the observed lines and line ratios with a grid of photodissociation region (PDR) models with different physical conditions, we derive the PDR cloud density n ≈ 2000 cm−3 and the far-ultraviolet ionizing radiation field G0≈ 200 (in units of the Habing field – the local Galactic interstellar radiation field of 1.6 × 10−6 W m−2). Using the CO-derived molecular mass and the PDR properties, we estimate the effective radius of the emitting region to be 500–700 pc. These characteristics are typical for star-forming, high-redshift galaxies. The radio observations indicate that SDP.81 deviates significantly from the local far-infrared/radio (FIR/radio) correlation, which hints that some fraction of the radio emission is coming from an active galactic nucleus (AGN). The constraints on the source size from millimetre-wave observations put a very conservative upper limit of the possible AGN contribution to less than 33 per cent. These indications, together with the high [O iii]/FIR ratio and the upper limit of [O i] 63 μm/[C ii] 158 μm, suggest that some fraction of the ionizing radiation is likely to originate from the AGN.

Teachers for rural schools - a challenge for South Africa

Abstract: Poverty is rife in many African countries and this has serious implications for the provision of quality education. Rural schools face severe challenges that are unique to their environment. A lack of parental interest in children’s education, insufficient funding from the state, a lack of resources, underqualified teachers, and multi-grade teaching are some of the barriers to effective education. These challenges can be attributed to numerous sources, from within school structures and from the external environment, including local communities and education authorities. After 25 years of democracy, educational standards and learner performance in rural schooling has shown little improvement. This study illustrates the complexity and inter-connectedness of the problems faced by teachers in South African rural schools. Using qualitative research within the interpretivist paradigm, this article explores the perceptions and experiences of teachers in rural schools located in White River in the Mpumalanga province. This grounded-theory research focuses on effective teaching and learning. The findings reveal that most rural schools do not have water, sanitation, or electricity, and classrooms are in a terrible state. These issues have serious implications for effective teaching and learning. Keywords: deployment; education level; education quality; recruitment; rural schools

Non-coding RNAs and Exosomes: Their Role in the Pathogenesis of Sepsis.

Abstract: Sepsis is characterized as an uncontrolled host response to infection, and it represents a serious health challenge, causing excess mortality and morbidity worldwide. The discovery of sepsis-related epigenetic and molecular mechanisms could result in improved diagnostic and therapeutic approaches, leading to a reduced overall risk for affected patients. Accumulating data show that microRNAs, non-coding RNAs, and exosomes could all be considered as novel diagnostic markers for sepsis patients. These biomarkers have been demonstrated to be involved in regulation of sepsis pathophysiology. However, epigenetic modifications have not yet been widely reported in actual clinical settings, and further investigation is required to determine their importance in intensive care patients. Further studies should be carried out to explore tissue-specific or organ-specific epigenetic RNA-based biomarkers and their therapeutic potential in sepsis patients.

Effect of low-level laser irradiation and epidermal growth factor on adult human adipose-derived stem cells.

Abstract: The study investigated the effects of low-level laser radiation and epidermal growth factor (EGF) on adult adipose-derived stem cells (ADSCs) isolated from human adipose tissue. Isolated cells were cultured to semi-confluence, and the monolayers of ADSCs were exposed to low-level laser at 5 J/cm2 using 636 nm diode laser. Cell viability and proliferation were monitored using adenosine triphosphate (ATP) luminescence and optical density at 0 h, 24 h and 48 h after irradiation. Application of low-level laser irradiation at 5 J/cm2 on human ADSCs cultured with EGF increased the viability and proliferation of these cells. The results indicate that low-level laser irradiation in combination with EGF enhances the proliferation and maintenance of ADSCs in vitro.

A poised fragment library enables rapid synthetic expansion yielding the first reported inhibitors of PHIP(2), an atypical bromodomain

Abstract: Research into the chemical biology of bromodomains has been driven by the development of acetyl-lysine mimetics. The ligands are typically anchored by binding to a highly conserved asparagine residue. Atypical bromodomains, for which the asparagine is mutated, have thus far proven elusive targets, including PHIP(2) whose parent protein, PHIP, has been linked to disease progression in diabetes and cancers. The PHIP(2) binding site contains a threonine in place of asparagine, and solution screening have yielded no convincing hits. We have overcome this hurdle by combining the sensitivity of X-ray crystallography, used as the primary fragment screen, with a strategy for rapid follow-up synthesis using a chemically-poised fragment library, which allows hits to be readily modified by parallel chemistry both peripherally and in the core. Our approach yielded the first reported hit compounds of PHIP(2) with measurable IC50 values by an AlphaScreen competition assay. The follow-up libraries of four poised fragment hits improved potency into the sub-mM range while showing good ligand efficiency and detailed structural data.