Showing papers by "University of Konstanz published in 1996"

On the relationship between mobbing factors, and job content, social work environment, and health outcomes

Abstract: This article analyses the relationship between mobbing, job characteristics, social environment variables, and psychological ill-health. The Leymann Inventory of Psychological Terrorization (LIFT) was factor analysed and led to seven factors in two samples of mobbing victims (N = 50 and N = 99): Mobbing by organizational measures, social isolation, attacking the victim's private life, attacking the victim's attitudes, physical violence, verbal aggression, and rumours. Mobbing was correlated with bad job content, a bad social environment, and psychological ill-health. The findings suggest that the more social support supervisors gave, the less the victims reported being shouted at, being constantly criticized, and receiving verbal threats. In contrast, the more social support the victims received from their colleagues the less they reported being socially isolated or being ridiculed with regard to their private life. Moreover, having private life attacked showed the strongest correlation with psyc...

Photoelectrochromic windows and displays

Abstract: PHOTOCHROMIC materials1,2 change colour on absorption of light, whereas electrochromic materials3,4 change colour in response to an electrically induced change in oxidation state. Both classes of materials are being investigated for potential applications in displays, imaging devices and 'smart' windows5–8,15,16. Here we describe an alternative route to such applications, in which an electrochromic film and a photovoltaic film form the two electrodes of an electrochemical cell. The resulting structure exhibits photochromism, but unlike conventional photochromic films, the light-absorption process (in the photovoltaic film) is separate from the coloration process (in the electrochromic film): both may therefore be optimized individually. Moreover, as the coloration process in our cells requires an external electrical current between the two electrodes, the optical state of the cell—transparent, absorbing or, in the case of non-uniform illumination, patterned—can be stored when the circuit is open, or changed when the electrodes are connected.

Exploring the Nature of the Beast: International Relations Theory and Comparative Policy Analysis Meet the European Union

Abstract: Recent work on the European Union (EU) indicates an emerging consensus that the boundaries between the ‘domestic’ and the ‘international’ spheres, as well as between the ‘state’ and ‘society’, have to be crossed when conceptualizing the EU. This article starts with reviewing the controversy between neofunctionalism and intergovernmentalism, arguing that it lacks some categories necessary to capture distinctive features of the EU. It then presents a framework combining insights from work on transnational politics by international relations scholars, on policy networks and Politikveflechtung (interlocking politics) by comparative policy analysis, and by historical institutionalism. Propositions are generated on the institutional and structural conditions under which network analysis is better suited than intergovernmentalism to capture the EU policy-making process. Finally, the article argues that social constructivism has to be taken more seriously in the study of the EU and that network analysis should move beyond rational choice models and incorporate theories of communicative action. More work is needed on the role of ideas and principled beliefs in the EU integration process.

Structural Basis of Light Harvesting by Carotenoids: Peridinin-Chlorophyll-Protein from Amphidinium carterae

Abstract: Peridinin-chlorophyll-protein, a water-soluble light-harvesting complex that has a blue-green absorbing carotenoid as its main pigment, is present in most photosynthetic dinoflagellates. Its high-resolution (2.0 angstrom) x-ray structure reveals a noncrystallographic trimer in which each polypeptide contains an unusual jellyroll fold of the α-helical amino- and carboxyl-terminal domains. These domains constitute a scaffold with pseudo-twofold symmetry surrounding a hydrophobic cavity filled by two lipid, eight peridinin, and two chlorophyll a molecules. The structural basis for efficient excitonic energy transfer from peridinin to chlorophyll is found in the clustering of peridinins around the chlorophylls at van der Waals distances.

Dewetting Modes of Thin Metallic Films: Nucleation of Holes and Spinodal Dewetting.

Abstract: We have studied the dewetting of thin liquid metal films (Au, Cu, Ni) on fused silica substrates which occurs after melting with a Q-switched laser pulse. Optical microscopy, scanning electron microscopy, and scanning near field acoustic microscopy reveal two distinctly different modes of the dewetting process: On one hand, we observe heterogeneous nucleation of ``dry'' circular patches, which grow in diameter during the melting period. On the other hand, an instability of the liquid film against the growth in amplitude of surface waves with a characteristic wavelength is observed, which we believe is the first observation of spinodal dewetting. In contrast, the final structure of the ruptured film depends on whether nucleation or spinodal dewetting is dominant.

Morphogenesis and mechanisms of penetration by plant pathogenic fungi

Abstract: Infection structures of phytopathogenic fungi are modified hyphae specialized for the invasion of plant tissues. Initial events are adhesion to the cuticle and directed growth of the germ tube on the plant surface. At the site of penetration, appressoria are often formed that may have melanized walls and develop high turgor pressure to support the penetration process. The penetration hypha accumulates components of the cytoskeleton in the tip and secretes a variety of cell wall‐degrading enzymes in a highly regulated fashion in order to penetrate the cuticle and the plant cell wall. This article reviews recent papers on the cytology, physiology, and molecular biology of the penetration process.

Abnormal myotonic dystrophy protein kinase levels produce only mild myopathy in mice

Abstract: Myotonic dystrophy (DM) is commonly associated with CTG repeat expansions within the gene for DM–protein kinase (DMPK). The effect of altered expression levels of DMPK, which is ubiquitously expressed in all muscle cell lineages during development, was examined by disrupting the endogenous Dmpk gene and overexpressing a normal human DMPK transgene in mice. Nullizygous (−/−) mice showed only inconsistent and minor size changes in head and neck muscle fibres at older age, animals with the highest DMPK transgene expression showed hypertrophic cardiomyopathy and enhanced neonatal mortality. However, both models lack other frequent DM symptoms including the fibre–type dependent atrophy, myotonia, cataract and male–infertility. These results strengthen the contention that simple loss– or gain–of–expression of DMPK is not the only crucial requirement for development of the disease.

Back to log phase: sigma S as a global regulator in the osmotic control of gene expression in Escherichia coli.

Abstract: It is now well established that the sigma S subunit of RNA polymerase is a master regulator in a complex regulatory network that governs the expression of many stationary-phase-inducible genes in Escherichia coli. In this review, more recent findings will be summarized that demonstrate that sigma S also acts as a global regulator for the osmotic control of gene expression, and actually does so in exponentially growing cells. Thus, many sigma S-dependent genes are induced during entry into stationary phase as well as in response to osmotic upshift. K+ glutamate, which accumulates in hyperosmotically stressed cells, seems to specifically stimulate the activity of sigma S-containing RNA polymerase at sigma S-dependent promoters. Moreover, osmotic upshift results in an elevated cellular sigma S level similar to that observed in stationary-phase cells. This increase is the result of a stimulation of rpoS translation as well as an inhibition of the turnover of sigma S, which in exponentially growing non-stressed cells is a highly unstable protein. Whereas the RNA-binding protein HF-I, previously known as a host factor for the replication of phage Q beta RNA, is essential for rpoS translation, the recently discovered response regulator RssB, and ClpXP protease, have been shown to be required for sigma S degradation. The finding that the histone-like protein H-NS is also involved in the control of rpoS translation and sigma S turnover, sheds new light on the function of this protein in osmoregulation. Finally, preliminary evidence suggests that additional stresses, such as heat shock and acid shock, also result in increased cellular sigma S levels in exponentially growing cells. Taken together, sigma S function is clearly not confined to stationary phase. Rather, sigma S may be regarded as a sigma factor associated with general stress conditions.

Electrospray Mass Spectrometry of Biomacromolecular Complexes with Noncovalent Interactions—New Analytical Perspectives for Supramolecular Chemistry and Molecular Recognition Processes

Abstract: The development of “soft” ionization methods in recent years has enabled substantial progress in the mass spectrometric characterization of macromolecules, in particular important biopolymers such as proteins and nucleic acids. In contrast to the still existing limitations for the determination of molecular weights by other ionization methods such as fast atom bombardment and plasma desorption, electrospray ionization (ESI) and matrix-assisted laser desorption have provided a breakthrough to macromolecules larger than 100 kDa. Whereas these methods have been successfully applied to determine the molecular weight and primary structure of biopolymers, the recently discovered direct characterization by ESI-MS of complexes containing noncovalent interactions (“noncovalent complexes”) opens new perspectives for supramolecular chemistry and analytical biochemistry. Unlike other ionization methods ESI-MS can be performed in homogeneous solution and under nearly physiological conditions of pH, concentration, and temperature. ESI mass spectra of biopolymers, particularly proteins, exhibit series of multiply charged macromolecular ions with charge states and distributions (“charge structures”) characteristic of structural states in solution, which enable a differentiation between native and denatured tertiary structures. In the first part of this article, fundamental principles, the present knowledge about ion formation mechanism(s) of ESI-MS, the relations between tertiary structures in solution and charge structures of macro-ions in the gas phase, and experimental preconditions for the identification of noncovalent complexes are described. The hitherto successful applications to the identification of enzyme–substrate and –inhibitor complexes, supramolecular protein–and protein–nucleotide complexes, double-stranded polynucleotides, as well as synthetic self-assembled complexes demonstrate broad potential for the direct analysis of specific noncovalent interactions. The present results suggest new applications for the characterization of supramolecular structures and molecular recognition processes that previously have not been amenable to mass spectrometry; for example, the sequence-specific oligomerization of polypeptides, antigen–antibody complexes, enzyme–and receptor–ligand interactions, and the evaluation of molecular specificity in combinatorial syntheses and self-assembled systems.

The RNA-binding protein HF-I, known as a host factor for phage Qbeta RNA replication, is essential for rpoS translation in Escherichia coli.

Abstract: The rpoS-encoded cr s subunit of RNA polymerase in Escherichia coil is a global regulatory factor involved in several stress responses. Mainly because of increased rpoS translation and stabilization of cr s, which in nonstressed cells is a highly unstable protein, the cellular ~r s content increases during entry into stationary phase and in response to hyperosmolarity. Here, we identify the hfq-encoded RNA-binding protein HF-I, which has been known previously only as a host factor for the replication of phage Q~ RNA, as an essential factor for rpoS translation. An hfq null mutant exhibits strongly reduced ~r s levels under all conditions tested and is deficient for growth phase-related and osmotic induction of ~r s. Using a combination of gene fusion analysis and pulse-chase experiments, we demonstrate that the hfq mutant is specifically impaired in rpoS translation. We also present evidence that the H-NS protein, which has been shown to affect rpoS translation, acts in the same regulatory pathway as HF-I at a position upstream of HF-I or in conjunction with HF-I. In addition, we show that expression and heat induction of the heat shock ~r factor cr 32 (encoded by rpoH) is not dependent on HF-I, although rpoH and rpoS are both subject to translational regulation probably mediated by changes in mRNA secondary structure. HF-I is the first factor known to be specifically involved in rpoS translation, and this role is the first cellular function to be identified for this abundant ribosome-associated RNA-binding protein in E. coli.

Alterations in the antioxidative system of suspension‐cultured soybean cells (Glycine max) induced by oxidative stress

Abstract: The objectives of this study were the changes of antioxidative key enzyme activities under stress conditions induced by a peroxidizing herbicide using photoheterotrophi-cally grown, suspension-cultured soybean celts (Glycine max L.). Within two days, 50 to 500 nM oxyfluorfen. a p-nitrodiphenyl ether herbicide, caused up to 100% inhibition of growth, while simultaneously, the chlorophyll was 25% to completely bleached. The major cellular antioxidants ascorbate and glutathione showed different responses. Under stress conditions with more than 250 nM oxyfluorfen, the cellular ascorbate- concentration was halved, whereas dehydroascorbate remained roughly constant. The glutathione content (approximately one-fifth of that of ascorbate in untreated control cells) increased nearly 3-fold in the presence of 250 nM oxyfluorfen. Under this condition, oxidized glutathione was 5 times above the control level. The specific activities of selected enzymes participating in cellular defence, namely ascor-bate peroxidase, glutathione reductase, rnonodehydroascorbate reductase. peroxidase and catalase increased by 40 to 70% with oxyfluorfen concentrations between 50 and 500 nM, while dehydroascorbate reductase showed a significant decrease. Glutathione transferase activity even increased 6-fold under oxyfluorfen stress.

The response regulator RssB controls stability of the sigma(S) subunit of RNA polymerase in Escherichia coli.

Abstract: The rpoS-encoded sigma(S) subunit of RNA polymerase is a central regulator in a regulatory network that governs the expression of many stationary phase-induced and osmotically regulated genes in Escherichia coli. sigma(S) is itself induced under these conditions due to an increase in rpoS transcription (only in rich media) and rpoS translation as well as a stabilization of sigma(S) protein which in growing cells is subject to rapid turnover. We demonstrate here that a response regulator, RssB, plays a crucial role in the control of the cellular sigma(S) content. rssB null mutants exhibit nearly constitutively high levels of sigma(S) and are impaired in the post-transcriptional growth phase-related and osmotic regulation of sigma(S). Whereas rpoS translational control is not affected, sigma(S) is stable in rssB mutants, indicating that RssB is essential for sigma(S) turnover. RssB contains a unique C-terminal output domain and is the first known response regulator involved in the control of protein turnover.

‘Shared job strain’: A new approach for assessing the validity of job stress measurements

Abstract: While problems of self-report measures of work stress have long been recognized, those of more ‘objective’ measures are often underestimated. Combining both in structural equation models yields more valid estimates, yet correlations with indicators of well-being or strain rarely exceed .30. To decide whether this is due to insufficient validity of instruments or to the multi-causal aetiology of well-being, the concept of ‘shared job strain’ is introduced. This is a latent variable, with individual symptoms of strain of two workers holding the same job as indicators. Thus, it represents the strain that these two workers have in common, while truly individual variance is removed. It should, therefore, show much higher correlations with job stressors than do individual symptoms of strain. To estimate stressors, self-reports of the two workers and of two independent observers are used as indicators. Four stressors explained two-thirds of the variance in ‘shared job strain’. It is concluded (a) that estimating latent job stressors on the basis of self-report and observer indicators yields highly valid measurement and (b) that the substantive argument is supported. There probably is an upper limit of 15 to 20 per cent variance in total strain symptoms that can be explained by job stressors.

On the Way towards High-Efficiency Thin Film Silicon Solar Cells by the "Micromorph" Concept

Abstract: Recently the authors have demonstrated that compensated or “midgap” intrinsic hydrogenated microcrystalline silicon (μc-Si:H), as deposited by the Very High Frequency Glow Discharge (VHF-GD) technique, can be used as active layer in p-i-n solar cells. Compared to amorphous silicon (a-Si:H), μc-Si:H was found to have a significantly lower energy bandgap ofaround 1 eV. The combination of both materials (two absorbers with different gap energies) leads to a “real” tandem cell structure, which was called the “micromorph” cell. Micromorph cells can make better use of the sun's spectrum in contrast to conventional double-stacked a-Si:H / a-Si:H tandems. The present study will show that the compensation technique (involving boron “microdoping”) used sofar for obtaining midgap μc-Si:H can be replaced by the application of a gas purifier. The use of this gas purifier has a beneficial influence on the transport properties of undoped intrinsic μc-Si:H. By this procedure, increased cell efficiencies in both, single microcrystalline silicon p-i-n as well as micromorph cells could be obtained. In the first case 7.7 % stable, and in the second case 13.1% initial efficiency could be achieved under AMI.5 conditions. Preliminary light-soaking experiments performed on the tandem cells indicate that microcrystalline silicon could contribute to an enhancement of the stable efficiency performance. Micromorph cell manufacturing is fully compatible to a-Si:H technology; however, its deposition rate is still too low. With further increase of the rate, a similar cost reduction potential like in a-Si:H technology can be extrapolated.

Quantum Statistics of the Squeezed Vacuum by Measurement of the Density Matrix in the Number State Representation.

Abstract: We report the reconstruction of the quantum state of squeezed vacuum generated by a continuous-wave optical parametric amplifier. Homodyne detection and tomographic reconstruction methods were used to obtain the density matrix in the Fock (number state) representation. The photon number distribution exhibits odd-even oscillations, a manifestation of the photon pair production in the second-order nonlinear medium.

Radiation induced apoptosis.

Abstract: The response to ionising radiation, in terms of level of cell killing, depends on a number of factors that may be grouped into those that are genetically controlled, radiation quality and dosage, and environmental factors. There is a range of genetically controlled cellular properties such as stage of differentiation, mutations in specific genes (such as p53 and bcl-2) and stage of transformation that will determine the ability of the target cell to enter apoptosis. The so-called normal cells, are usually more radiosensitive and the majority of the cell population will enter into an apoptotic death. However, in response to high doses of ionising radiation and complex DNA damage as produced by high-LET radiation, an increased fraction of these cells will die by necrosis. There are several examples of environmental factors with relevance for the combined action of radiation and xenobiotics on carcinogenesis and in tumour therapy. In the case of normal cells, agents such as growth factors and tumour promoters, may decrease radiosensitivity. For certain type of tumour cells, radiation sensitivity can be increased in the presence of agents such as hormones, and the cells may die an apoptotic death. Removal of heavily compromised cells is essential to prevent a potential spreading of mutated clones. However, if apoptosis is inhibited (e.g., by tumour promoter), an increased fraction of damaged cells carrying genotoxic lesions may survive. This would significantly increase the risk of proliferation of precancerous cells. As discussed above, it is probably incorrect to make predictions about relative radiosensitivity based solely on mode of death. Intrinsic characteristics deriving from the cell type of origin of a line may be more important in determining radiosensitivity. The rapidly increasing knowledge about the process of radiation induced apoptosis has opened new frontiers in radiation biology, genetic toxicology, and cancer therapy and strongly motivates further research in this field.

Dewetting at the liquid-liquid interface.

Abstract: The dewetting behavior of a liquid film from a liquid substrate has been studied as a function of the substrate viscosity, using two highly viscous polymers as a model system. The dewetting velocity exhibits a minimum as a function of substrate viscosity. This behavior results from the competition of (1) the mass transport in the substrate, and (2) the retarded deformation of the liquid-liquid interface. Atomic force microscopy is used to image both the liquid-air interfaces and the buried liquid-liquid interface. The shape of the latter changes significantly with increasing substrate viscosity.

Videomicroscopy of methacholine-induced contraction of individual airways in precision-cut lung slices

Abstract: Contraction of airways of different size can be studied in viable lung slices by videomicroscopy. However, at present, application of this technique is limited by the heterogeneous responses obtained. We investigated the use of precision-cut lung slices to examine contraction of individual airways. Lung slices of 250 +/- 20 microns were prepared from Wistar rats and cultured in a roller incubator in serum-free minimum essential medium (MEM). Under these conditions, the slices were viable for at least 70 h, as indicated by leakage of lactate dehydrogenase into the supernatant, thymidine incorporation and ciliary beating. The slices were placed in a newly developed incubation chamber and mounted by a nylon thread that was fixed to a platinum wire. The whole chamber was positioned on a microscope stage, and contraction of single airways was followed under a microscope that was coupled to a CCD-camera. Reduction in airway area was taken as an index of bronchoconstriction and was determined by a computer program. Addition of methacholine resulted in a concentration-dependent (concentration producing half the maximal effect (EC50) = 0.64 +/- 0.08 (mean +/- SD) microM; n = 64) contraction of single airways. In the presence of hydrocortisone, the EC50 was about six times greater, i.e. 3.7 +/- 0.9 microM (n = 7), and the effect of the steroid was largely abolished by propanolol (EC50 = 1.1 +/- 0.1 microM; n = 7). Airways with an area smaller than 35,000 microns2 were nearly nine times more sensitive to methacholine (EC50 = 0.1 +/- 0.03 microM; n = 20) than larger ones (EC50 = 87 +/- 0.27 microM; n = 22). We conclude that cultured precision-cut lung slices are a useful model for routine study of contraction of individual airways of various sizes. The measurements were precise and reproducible and showed that smaller airways are more sensitive to methacholine than larger ones.

Recovering Probability Distributions from Option Prices

Abstract: This paper derives underlying asset risk-neutral probability distributions of European options on the S&P 500 index. Nonparametric methods are used to choose probabilities which minimize an objective function subject to requiring that the probabilities are consistent with observed option and underlying asset prices. Alternative optimization specifications produce approximately the same implied distributions. A new and fast optimization technique for estimating probability distributions based on maximizing the smoothness of the resulting distribution is proposed. Since the crash, the risk-neutral probability of a three (four) standard deviation decline in the index (about-36% (-46%) over a year) is about 10 (100) times more likely than under the assumption of lognormality.

Visually induced gamma-band responses in human electroencephalographic activity : a link to animal studies

Abstract: Visual presentation of an object produces firing patterns in cell assemblies representing the features of the object. Based on theoretical considerations and animal experiments, it has been suggested that the binding of neuronal representations of the various features is achieved through synchronization of the oscillatory firing patterns. The present study demonstrates that stimulus-induced gamma-band responses can be recorded non-invasively from human subjects attending to a single moving bar. This finding indicates the synchronization of oscillatory activity in a large group of cortical neurons. Gamma-band responses were not as apparent in the presence of two independently moving stimuli, suggesting that the neuronal activity patterns of different objects are not synchronized. These results open a new paradigm for investigating the mechanisms of feature binding and association building in relation to subjective perception.

Identification in situ and dynamics of bacteria on limnetic organic aggregates (lake snow).

Abstract: Microbial assemblages on large organic aggregates (lake snow) of Lake Constance, Germany, were analyzed with rRNA-directed fluorescent oligonucleotide probes specific for the domain Bacteria and the alpha-, beta-, and gamma-subclasses of the class Proteobacteria. Lake snow aggregates were either collected in situ by SCUBA diving or in a sediment trap at 50 m or formed of natural lake water incubated in rolling cylinders under simulated in situ conditions. For the latter aggregates, the time course of the microbial colonization was also examined. The natural aggregates and those made in rolling cylinders were composed of the particulate organic material present in the lake and thus reflected the composition of the ambient plankton community. All types of lake snow aggregates examined were heavily colonized by microbial cells and harbored between 0.5 x 10(6) and > 2 x 10(6) cells aggregate -1. Between 55 and 100% of the microbial cells stained with 4', 6-diamidino-2-phenylindole (DAPI) could be visualized with the domain Bacteria-specific probe. In most samples, beta-subclass proteobacteria dominated the microbial community, constituting 27 to 42% of total cells as counted by DAPI staining, irrespective of the composition of the aggregates. During the time course experiments with the laboratory-made aggregates, the fraction of beta-subclass proteobacteria usually increased over time. Except for a few samples, alpha- and gamma-subclass proteobacteria were far less abundant than beta-subclass proteobacteria, constituting 11 to 25 and 9 to 33% of total cells, respectively. Therefore, we assume that a specific aggregate-adapted microbial community was established on the aggregates. Because the compositions of the microbial assemblages on natural and laboratory-made aggregates were similar, we conclude that aggregates made in rolling cylinders are good model system with which to examine the formation and microbial colonization of macroscopic organic aggregates.

Posttranscriptional osmotic regulation of the sigma(s) subunit of RNA polymerase in Escherichia coli.

Abstract: The sigma(s) subunit of RNA polymerase (encoded by the rpoS gene) is a master regulator in a complex regulatory network that governs the expression of many stationary-phase-induced and osmotically regulated genes in Escherichia coli. rpoS expression is itself osmotically regulated by a mechanism that operates at the posttranscriptional level. Cells growing at high osmolarity already exhibit increased levels of sigma(s) during the exponential phase of growth. Osmotic induction of rpoS can be triggered by addition of NaCl or sucrose and is alleviated by glycine betaine. Stimulation of rpoS translation and a change in the half-life of sigma(s) from 3 to 50 min both contribute to osmotic induction. Experiments with lacZ fusions inserted at different positions within the rpoS gene indicate that an element required for sigma(s) degradation is encoded between nucleotides 379 and 742 of the rpoS coding sequence.