Institution
University of Lisbon
Education•Lisbon, Lisboa, Portugal•
About: University of Lisbon is a education organization based out in Lisbon, Lisboa, Portugal. It is known for research contribution in the topics: Population & European union. The organization has 19122 authors who have published 48503 publications receiving 1102623 citations. The organization is also known as: Universidade de Lisboa & Lisbon University.
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221 citations
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TL;DR: Results suggest that EEG complexity, as indexed by MSE measures, may also be a marker for disturbances in task-specific processing of information in people with autism.
220 citations
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TL;DR: A new approximate FRAP model is presented that takes into account diffusion of highly mobile molecules during the bleach phase and shows that by the time the first postbleach image is acquired in a CLSM a significant fluorescence recovery of fast-moving molecules has already taken place.
Abstract: Fluorescence recovery after photobleaching (FRAP) is a widely used tool for estimating mobility parameters of fluorescently tagged molecules in cells Despite the widespread use of confocal laser scanning microscopes (CLSMs) to perform photobleaching experiments, quantitative data analysis has been limited by lack of appropriate practical models Here, we present a new approximate FRAP model for use on any standard CLSM The main novelty of the method is that it takes into account diffusion of highly mobile molecules during the bleach phase In fact, we show that by the time the first postbleach image is acquired in a CLSM a significant fluorescence recovery of fast-moving molecules has already taken place The model was tested by generating simulated FRAP recovery curves for a wide range of diffusion coefficients and immobile fractions The method was further validated by an experimental determination of the diffusion coefficient of fluorescent dextrans and green fluorescent protein The new FRAP method was used to compare the mobility rates of fluorescent dextrans of 20, 40, 70, and 500 kDa in aqueous solution and in the nucleus of living HeLa cells Diffusion coefficients were lower in the nucleoplasm, particularly for higher molecular weight dextrans This is most likely caused by a sterical hindrance effect imposed by nuclear components Decreasing the temperature from 37 to 22 degrees C reduces the dextran diffusion rates by approximately 30% in aqueous solution but has little effect on mobility in the nucleoplasm This suggests that spatial constraints to diffusion of dextrans inside the nucleus are insensitive to temperature
220 citations
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220 citations
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TL;DR: In response to the World Health Organization (WHO) statements and international concerns regarding the coronavirus disease 2019 (COVID‐19) outbreak, FIGO has issued comprehensive guidance for the management of pregnant women.
220 citations
Authors
Showing all 19716 results
Name | H-index | Papers | Citations |
---|---|---|---|
Joao Seixas | 153 | 1538 | 115070 |
A. Gomes | 150 | 1862 | 113951 |
Marco Costa | 146 | 1458 | 105096 |
António Amorim | 136 | 1477 | 96519 |
Osamu Jinnouchi | 135 | 885 | 86104 |
P. Verdier | 133 | 1111 | 83862 |
Andy Haas | 132 | 1096 | 87742 |
Wendy Taylor | 131 | 1252 | 89457 |
Steve McMahon | 130 | 878 | 78763 |
Timothy Andeen | 129 | 1069 | 77593 |
Heather Gray | 129 | 966 | 80970 |
Filipe Veloso | 128 | 887 | 75496 |
Nuno Filipe Castro | 128 | 960 | 76945 |
Oliver Stelzer-Chilton | 128 | 1141 | 79154 |
Isabel Marian Trigger | 128 | 974 | 77594 |