Showing papers by "University of Ljubljana published in 2000"

A Nonlinear Analysis Method for Performance‐Based Seismic Design

Abstract: A relatively simple nonlinear method for the seismic analysis of structures (the N2 method) is presented. It combines the pushover analysis of a multi‐degree‐of‐freedom (MDOF) model with the response spectrum analysis of an equivalent single‐degree‐of‐freedom (SDOF) system. The method is formulated in the acceleration‐displacement format, which enables the visual interpretation of the procedure and of the relations between the basic quantities controlling the seismic response. Inelastic spectra, rather than elastic spectra with equivalent damping and period, are applied. This feature represents the major difference with respect to the capacity spectrum method. Moreover, demand quantities can be obtained without iteration. Generally, the results of the N2 method are reasonably accurate, provided that the structure oscillates predominantly in the first mode. Some additional limitations apply. In the paper, the method is described and discussed, and it basic derivations are given. The similarities a...

Y-Chromosomal Diversity in Europe Is Clinal and Influenced Primarily by Geography, Rather than by Language

Abstract: Clinal patterns of autosomal genetic diversity within Europe have been interpreted in previous studies in terms of a Neolithic demic diffusion model for the spread of agriculture; in contrast, studies using mtDNA have traced many founding lineages to the Paleolithic and have not shown strongly clinal variation. We have used 11 human Y-chromosomal biallelic polymorphisms, defining 10 haplogroups, to analyze a sample of 3,616 Y chromosomes belonging to 47 European and circum-European populations. Patterns of geographic differentiation are highly nonrandom, and, when they are assessed using spatial autocorrelation analysis, they show significant clines for five of six haplogroups analyzed. Clines for two haplogroups, representing 45% of the chromosomes, are continentwide and consistent with the demic diffusion hypothesis. Clines for three other haplogroups each have different foci and are more regionally restricted and are likely to reflect distinct population movements, including one from north of the Black Sea. Principal-components analysis suggests that populations are related primarily on the basis of geography, rather than on the basis of linguistic affinity. This is confirmed in Mantel tests, which show a strong and highly significant partial correlation between genetics and geography but a low, nonsignificant partial correlation between genetics and language. Genetic-barrier analysis also indicates the primacy of geography in the shaping of patterns of variation. These patterns retain a strong signal of expansion from the Near East but also suggest that the demographic history of Europe has been complex and influenced by other major population movements, as well as by linguistic and geographic heterogeneities and the effects of drift.

Cultural variation of leadership prototypes across 22 European countries

Abstract: This study sets out to test the assumption that concepts of leadership differ as a function of cultural differences in Europe and to identify dimensions which describe differences in leadership concepts across European countries. Middle-level managers (N = 6052) from 22 European countries rated 112 questionnaire items containing descriptions of leadership traits and behaviours. For each attribute respondents rated how well it fits their concept of an outstanding business leader. The findings support the assumption that leadership concepts are culturally endorsed. Specifically, clusters of European countries which share similar cultural values according to prior cross-cultural research (Ronen & Shenkar, 1985), also share similar leadership concepts. The leadership prototypicality dimensions found are highly correlated with cultural dimensions reported in a comprehensive cross-cultural study of contemporary Europe (Smith, Dugan, & Trompenaars, 1996). The ordering of countries on the leadership dimensions is considered a useful tool with which to model differences between leadership concepts of different cultural origin in Europe. Practical implications for cross-cultural management, both in European and non-European settings, are discussed.

Production of actin‐specific ADP‐ribosyltransferase (binary toxin) by strains of Clostridium difficile

Abstract: In addition to the two large clostridial cytotoxins (TcdA and TcdB) certain strains of Clostridium difficile produce an actin-specific ADP-ribosyltransferase, or binary toxin. PCR reactions were developed to detect genes encoding the enzymatic (cdtA) and binding (cdtB) components of the binary toxin and 170 representative strains were tested to assess the prevalence of the toxin. Positive PCR results (n=59) were confirmed by immunoblotting and ADP-ribosyltransferase assay. PCR ribotype and toxinotype (restriction fragment length polymorphism analysis of genes for TcdA and TcdB) correlated with possession of binary toxin genes. All strains with cdtA and cdtB belonged to toxin-variable toxinotypes and five toxin-producing groups of strains have been described according to the presence or absence of TcdA, TcdB and binary toxin. Result indicate that ca. 6.4% of toxigenic isolates of C. difficile referred to the Anaerobe Reference Unit from UK hospitals have cdtA and cdtB genes.

Strength and reliability of surface treated Y‐TZP dental ceramics

Abstract: This work was undertaken to evaluate the effects of dental grinding and sandblasting on the biaxial flexural strength and Weibull modulus of various Y-TZP ceramics containing 3 mol% yttria. In addition, the susceptibility of pristine and mechanically treated materials to low-temperature degradation under the conditions adopted for testing the chemical solubility of dental ceramics was investigated. The results revealed that surface grinding and sandblasting exhibit a counteracting effect on the strength of Y-TZP ceramics. Dental grinding lowered the mean strength and Weibull modulus, whereas sandblasting provided a powerful method for strengthening, but at the expense of somewhat lower reliability. The finest-grained material exhibited the highest strength after sintering, but it was less damage tolerant than tougher, coarse-grained materials. Upon extraction with the acetic acid solution and the ammonia solution, a significant amount of tetragonal zirconia had transformed to monoclinic, but extensive microcracking and attendant strength degradation had not yet occurred. Standard grade Y-TZP ceramics are more resistant in an alkaline than in an acidic environment, and there was a strong grain-size dependence of the diffusion-controlled transformation. Since a special Y-TZP grade containing a small amount of alumina exhibited the highest damage tolerance and superior stability in an acidic environment, this material shows considerable promise for dental applications.

Eigenvector 1: An Optimal Correlation Space for Active Galactic Nuclei.

Abstract: We identify a correlation space involving optical and UV emission-line parameters as well as the soft X-ray spectral index that provides optimal discrimination between all principal classes of active galactic nuclei (AGNs). Most of the sources in our three high-quality data samples show a strong intercorrelation with narrow-line Seyfert 1 (NLSy1) galaxies and steep-spectrum radio galaxies occupying opposite extrema in the space. NLSy1 sources show a clear continuity with broader line sources, indicating that they are not a disjoint class of AGN as is sometimes suggested. We interpret the principal intercorrelation in the parameter space as being driven by the AGN luminosity-to-black hole mass ratio (L/M is proportional to the Eddington ratio). Source orientation no doubt also plays an important role, but it is not yet clear whether FWHM Hβ or C IV λ1549 line shift is the better indicator. We tentatively identify two radio-quiet populations: an almost pure radio-quiet population A, with FWHM ≤ 4000, and population B, which occupies the same parameter domain as the flat-spectrum radio-loud sources. A possible interpretation sees population A/NLSy1 as lower mass/high accretion rate sources and population B/radio-loud sources as the opposite.

Response to Antihypertensive Therapy in Older Patients With Sustained and Nonsustained Systolic Hypertension

Abstract: Background—The goal of the present study was to assess the effect of antihypertensive therapy on clinic (CBP) and ambulatory (ABP) blood pressures, on ECG voltages, and on the incidence of stroke and cardiovascular events in older patients with sustained and nonsustained systolic hypertension. Methods and Results—Patients who were ≥60 years old, with systolic CBP of 160 to 219 mm Hg and diastolic CBP of <95 mm Hg, were randomized into the double-blind placebo-controlled Systolic Hypertension in Europe (Syst-Eur) Trial. Treatment consisted of nitrendipine, with the possible addition of enalapril, hydrochlorothiazide, or both. Patients enrolled in the Ambulatory Blood Pressure Monitoring Side Project were classified according to daytime systolic ABP into 1 of 3 subgroups: nonsustained hypertension (<140 mm Hg), mild sustained hypertension (140 to 159 mm Hg), and moderate sustained hypertension (≥160 mm Hg). At baseline, patients with nonsustained hypertension had smaller ECG voltages (P<0.001) and, during f...

The prevalence of oral mucosal lesions in a population in Ljubljana, Slovenia.

Abstract: The purpose of the present study was to evaluate the prevalence of oral mucosal lesions and conditions in a population in Ljubljana, capital of Slovenia. A total 1609 subjects represented the study population in the survey about the periodontal treatment needs in a population in Ljubljana, conducted from 1983 to 1987. Ten years later the same 1609 subjects were invited to the second examination. Altogether, 555 (34.5%) of the invited subjects in the age range 25-75 years came for an interview and clinical examination at the Department of Oral Medicine and Periodontology of the Dental Clinic in Ljubljana. Oral mucosal lesions and conditions were evaluated according to the WHO Guide to Epidemiology and Diagnosis of Oral Mucosal Diseases and Conditions. The results showed the presence of one or more mucosal lesions in 61.6% of the population. Fordyce's condition was observed the most frequently (49.7%) followed by: fissured tongue (21.1%), varices (16.2%), history of herpes labialis (16.0%), history of recurrent aphthae (9.7%), denture stomatitis (4.3%), leukoplakia (3.1%), cheek biting (2.7%), lichen planus (2.3%), frictional keratosis (2.2%), geographic tongue (2.2%), geographic and fissured tongue together (1.1%), mucocele (0.9%), smoker's palate (0.5%) and angular chelitis (0.4%). In the population examined, no oral malignancies were observed. Mucosal lesions like whitish lesions, denture related lesions, fissured tongue, varices and mucocele were more prevalent with increasing age. Tobacco-related lesions (leukoplakia and smoker's palate together) were significantly more prevalent among men than among women (P<0.05), while lichen planus, denture stomatitis and herpes labialis occurred more frequently in the female population.

Reduction and Oxidation Processes of Chromium in Soils

Abstract: The kinetics of reduction and oxidation of soluble chromium in various soils (peat, clay, sand, and luvi-chromic cambisols) were studied at constant soil moisture and constant temperature. A total ...

Electrochemotherapy with Cisplatin: Clinical Experience in Malignant Melanoma Patients

Abstract: Electrochemotherapy consists of chemotherapy followed by local application of electric pulses to the tumor to increase drug delivery into the cells. The aim of this Phase II clinical study was to evaluate the antitumor effectiveness of electrochemotherapy using intratumoral cisplatin administration on cutaneous tumor nodules in malignant melanoma patients. In 10 patients, 133 tumor nodules of different sizes were treated: (a) 82 tumor nodules were treated with electrochemotherapy; (b) 27 tumor nodules were treated with cisplatin; (c) 2 tumor nodules were treated with electric pulses; and (d) 22 tumor nodules were untreated. Four weeks after therapy, 78% objective responses were obtained in the electrochemotherapy group, and 38% objective responses were obtained in the cisplatin group. Exposure of tumor nodules to electric pulses without cisplatin treatment had no effect on tumor growth. Electrochemotherapy was well tolerated by all patients, and a good cosmetic effect was obtained, with only minimal scarring and a slight depigmentation of the skin. At 124 weeks of follow-up, a 77% control rate of the tumor nodules treated by electrochemotherapy was observed, compared to 19% for those that were treated with cisplatin only (P < 0.0001). Our results clearly demonstrate that electrochemotherapy with cisplatin is a highly effective approach for treatment of cutaneous malignant melanoma nodules. The advantages of this therapy include its simplicity, the short duration of treatment sessions, low cisplatin doses, and insignificant side effects, as well as the fact that it can be done on an outpatient basis.

Theoretical evaluation of the distributed power dissipation in biological cells exposed to electric fields.

Abstract: The paper deals with the power dissipation caused by exposure of biological cells to electric fields of various frequencies. With DC and sub-MHz AC frequencies, power dissipation in the cell membrane is of the same order of magnitude as in the external medium. At MHz and GHz frequencies, dielectric relaxation leads to dielectric power dissipation gradually increasing with frequency, and total power dissipation within the membrane rises significantly. Since such local increase can lead to considerable biochemical and biophysical changes within the membrane, especially at higher frequencies, the bulk treatment does not provide a complete picture of effects of an exposure. In this paper, we theoretically analyze the distribution of power dissipation as a function of field frequency. We first discuss conductive power dissipation generated by DC exposures. Then, we focus on AC fields; starting with the established first-order model, which includes only conductive power dissipation and is valid at sub-MHz frequencies, we enhance it in two steps. We first introduce the capacitive properties of the cytoplasm and the external medium to obtain a second-order model, which still includes only conductive power dissipation. Then we enhance this model further by accounting for dielectric relaxation effects, thereby introducing dielectric power dissipation. The calculations show that due to the latter component, in the MHz range the power dissipation within the membrane significantly exceeds the value in the external medium, while in the lower GHz range this effect is even more pronounced. This implies that even in exposures that do not cause a significant temperature rise at the macroscopic, whole-system level, the locally increased power dissipation in cell membranes could lead to various effects at the microscopic, single-cell level.

Lifting Graph Automorphisms by Voltage Assignments

Abstract: The problem of lifting graph automorphisms along covering projections and the analysis of lifted groups is considered in a purely combinatorial setting. The main tools employed are: (1) a systematic use of the fundamental groupoid; (2) unification of ordinary, relative and permutation voltage constructions into the concept of a voltage space; (3) various kinds of invariance of voltage spaces relative to automorphism groups; and (4) investigation of geometry of the lifted actions by means of transversals over a localization set. Some applications of these results to regular maps on surfaces are given. Because of certain natural applications and greater generality, graphs are allowed to have semiedges. This requires careful re-examination of the whole subject and at the same time leads to simplification and generalization of several known results.

Cobalt and Copper Hexacyanoferrate Modified Carbon Fiber Microelectrode as an All‐Solid Potentiometric Microsensor for Hydrazine

Abstract: This article reports on the novel mixed cobalt and copper hexacyanoferrate (CoCuHCF)-modified carbon fiber cylinder microelectrode (CFCME) and its application to potentiometric determination of highly toxic hydrazine. The substrate CFCMEs were fabricated in a standard manner using carbon fibers of 7 µm in diameter. The CoCuHCF film was deposited electrochemically by cycling the potential between 0 and +1.0 V (vs. Ag/AgCl) in a solution containing the precursor salts. It exhibited good chemical stability in the pH range from 1 to 9. The effects of the coverage/thickness of the CoCuHCF coating, of the pH of a measurement solution, and of the activation of the sensor, on the potentiometric response to hydrazine were examined in detail. The potentiometric behavior of CoCuHCF film in the presence of hydrazine, with the potential (emf) slope of –55 mV/decade, was compared with those of four single metal HCF films (CoHCF, CuHCF, FeHCF, and NiHCF) prepared in the same way, and the possible role of the CoCuHCF film components is explored and discussed. The emf versus log CHyd calibration plot was linear over 3 orders of magnitude, from 1.0×10–6 to 1.0×10–3 mol/L, with a correlation coefficient of 0.995. The limit of detection was found to be 5×10–7 mol/L. The response times of 10 to 30 s and the temperature coefficients of ca–2.7 mV/°C were obtained at various hydrazine concentrations. The repeatability of measurements was very good with the relative standard deviation ranging from 3 to 4% (n=10), depending on hydrazine concentration. The selectivity of the reported microsensor was found excellent, except in the case of a few negatively charged interferents for which it was greately improved by an additional Nafion coating. The useful lifetime of the all-solid microsensor was found to be more than 2 months when stored in air.

Dielectric dispersion of the relaxor PLZT ceramics in the frequency range 20 Hz-100 THz

Abstract: The dielectric dispersion of the transparent relaxor ferroelectric ceramics PLZT 8/65/35 and 9.5/65/35 was determined in a wide frequency range including the microwave and infrared range. The number of observed polar phonons in infrared spectra gives evidence about the locally broken cubic symmetry and the presence of polar nanoclusters in the whole investigated temperature range up to 530 K. A single broad and symmetric dispersion that occurs below the polar phonon frequencies was fitted with the Cole-Cole formula and a uniform distribution of Debye relaxations. On decreasing temperature, the distribution of relaxation times becomes extremely broad which indicates increasing correlation among the clusters. The mean relaxation time diverges according to the Vogel-Fulcher law with the same freezing temperature 230±5 K for both ceramics, but different activation energies 1370 K and 1040 K for the 8/65/35 and 9.5/65/35 sample, respectively. The shortest relaxation time is about 10-12 s and remains almost temperature independent. Below room temperature, the loss spectra become essentially frequency independent and the permittivity increases linearly with decreasing logarithm of frequency. The slope of this dependence is proportional to T 4 in the investigated temperature range (above 210 K) which indicates appreciable anharmonicity of the potential for polarization fluctuations.

Construction of large-volume monolithic columns.

Abstract: Monolithic supports have become the subject of extensive study in the past years. Despite their advantageous features and many successful chromatographic applications in the analytical scale, only a very few examples of larger volume monoliths were described. In the case of GMA−EDMA monoliths, this can be attributed to the fact that due to the exothermic polymerization a pronounced temperature increase inside the monolith significantly affects the structure. The temperature increase depends on the thickness of the monolith, and consequently, there is an upper limit that allows the preparation of a unit with a uniform structure. In the present work, we have analyzed a heat release during the polymerization and have derived a mathematical model for the prediction of the maximal thickness of the monolithic annulus having a uniform structure. On the basis of the calculations, two annuluses of different diameters were polymerized and merged into a single monolithic unit with a volume of 80 mL. In addition, a s...

Reversible transitions between synchronization states of the cardiorespiratory system.

Abstract: Phase synchronization between cardiac and respiratory oscillations is investigated during anesthesia in rats. Synchrograms and time evolution of synchronization indices are used to show that the system passes reversibly through a sequence of different phase-synchronized states as the anesthesia level changes, indicating that it can undergo phase transitionlike phenomena. It appears that the synchronization state may be used to characterize the depth of anesthesia.

Black fungi: clinical and pathogenic approaches.

Abstract: Data are presented on the clinically relevant black yeasts and their relatives, i.e., members of the Ascomycete order Chaetothyriales. In order to understand the pathology of these fungi it is essential to know their natural ecological niche. From a relatively low degree of molecular variability of the black yeast Exophiala dermatitidis, potential agent of brain infections in patients from East Asia, it is concluded that this species is an emerging pathogen, currently going through a process of active speciation. It is found to be an oligotrophic fungus in hot, moist environments, such as steambaths. Cladophialophora -, Fonsecaea - and Ramichloridium -like strains, known in humans as agents of chromoblastomycosis, are frequently found on rotten plant material, but the fungal molecular diversity in the environment is much higher than that on the human patient, so that it is difficult to trace the etiological agents of the disease with precision. This approach has been successful with Cladophialophora carri...

Secondary intracranial meningiomas after high-dose cranial irradiation: report of five cases and review of the literature.

Abstract: Purpose: To review cases of secondary intracranial meningiomas following high-dose cranial irradiation (≥ 10 Gy) identified in Slovenia between 1968 and 1998, to determine their histological profile and to review the literature on this topic. Methods and Materials: Personal files of patients treated for secondary intracranial meningioma during a 31-year period were reviewed. In cases which met the criteria for radiation-induced tumors, steroid hormone receptor and Ki-67 status were analyzed. For the literature review, computerized database systems and reference lists from respective publications were used. Results: Five patients (2 females, 3 males), 3–11 years old at the time of cranial irradiation, developed secondary meningioma after a latency period of 9.5–31.5 years. Three patients had multiple tumors and 2 developed recurrent disease. Of 9 histologically examined tumors, 5 were graded as benign and 4 as atypical meningiomas, with Ki-67 proliferative index 3.2 ± 3.6 and 10 ± 6, respectively. The ratio between positive and negative meningiomas regarding immunostaining for progesterone and estrogen receptors was eight-to-one and six-to-three, respectively. Cumulative actuarial risk of secondary meningioma in a cohort of 445 children 16 years or younger treated with high-dose cranial irradiation between 1968 and 1990 in Slovenia at 10, 20, and 25 years was 0.53%, 1.2%, and 8.18%, respectively. Out of 126 cases of radiation-induced meningiomas reported, 57% were females and 43% were males, with mean age at presentation 33 ± 17.3 years. The majority (68%) of patients was irradiated during childhood. The latency period was significantly shorter in those who aged 5 years or less at the time of cranial irradiation ( p = 0.04), and in those with atypical/anaplastic tumor ( p = 0.01). Correlation between radiation dose and latency period could not be found. Conclusion: Secondary meningiomas following high-dose cranial irradiation are characterized by younger age at presentation, by higher male-to-female ratio and by biologically more aggressive variants compared to primary spontaneous meningiomas. Latency period correlated with the age at the time of cranial irradiation and with tumor grade but not with irradiation dose. Ki-67 immunoreactivity correlated with histological grade. The progesterone and estrogen receptor immunoreactivity was high. The risk for development of secondary meningioma after high-dose cranial irradiation was increasing with the time of follow-up.

Retrospective shading correction based on entropy minimization.

Abstract: Shading is a prominent phenomenon in microscopy, manifesting itself via spurious intensity variations not present in the original scene. The elimination of shading effects is frequently necessary for subsequent image processing tasks, especially if quantitative analysis is the final goal. While most of the shading effects may be minimized by setting up the image acquisition conditions carefully and capturing additional calibration images, object-dependent shading calls for retrospective correction. In this paper a novel method for retrospective shading correction is proposed. Firstly, the image formation process and the corresponding shading effects are described by a linear image formation model, which consists of an additive and a multiplicative parametric component. Secondly, shading correction is performed by the inverse of the image formation model, whose shading components are estimated retrospectively by minimizing the entropy of the acquired images. A number of tests, performed on artificial and real microscopical images, show that this approach is efficient for a variety of differently structured images and as such may have applications in and beyond the field of microscopical imaging.

Synchronization and modulation in the human cardiorespiratory system

Abstract: We analyse phase and frequency synchronization in the human cardio-respiratory system. The method for analysis of noisy nonstationary bivariate data is applied to simultaneously measured cardiac and respiratory activity. Short epochs of phase and/or frequency locking between respiratory and cardiac rhythms are detected in healthy relaxed subjects (non-athletes). We reveal that the strength of phase synchronization is inversely related to the extent of respiratory modulation of the heart rate.