University of Lorraine

About: University of Lorraine is a education organization based out in Nancy, France. It is known for research contribution in the topics: Population & Nonlinear system. The organization has 11942 authors who have published 25010 publications receiving 425227 citations. The organization is also known as: Lorraine University.

Inhibition of Histone Deacetylase 3 Causes Replication Stress in Cutaneous T Cell Lymphoma

Abstract: Given the fundamental roles of histone deacetylases (HDACs) in the regulation of DNA repair, replication, transcription and chromatin structure, it is fitting that therapies targeting HDAC activities are now being explored as anti-cancer agents. In fact, two histone deacetylase inhibitors (HDIs), SAHA and Depsipeptide, are FDA approved for single-agent treatment of refractory cutaneous T cell lymphoma (CTCL). An important target of these HDIs, histone deacetylase 3 (HDAC3), regulates processes such as DNA repair, metabolism, and tumorigenesis through the regulation of chromatin structure and gene expression. Here we show that HDAC3 inhibition using a first in class selective inhibitor, RGFP966, resulted in decreased cell growth in CTCL cell lines due to increased apoptosis that was associated with DNA damage and impaired S phase progression. Through isolation of proteins on nascent DNA (iPOND), we found that HDAC3 was associated with chromatin and is present at and around DNA replication forks. DNA fiber labeling analysis showed that inhibition of HDAC3 resulted in a significant reduction in DNA replication fork velocity within the first hour of drug treatment. These results suggest that selective inhibition of HDAC3 could be useful in treatment of CTCL by disrupting DNA replication of the rapidly cycling tumor cells, ultimately leading to cell death.

The Obscure World of Integrative and Mobilizable Elements, Highly Widespread Elements that Pirate Bacterial Conjugative Systems.

Abstract: Conjugation is a key mechanism of bacterial evolution that involves mobile genetic elements. Recent findings indicated that the main actors of conjugative transfer are not the well-known conjugative or mobilizable plasmids but are the integrated elements. This paper reviews current knowledge on integrative and mobilizable elements (IMEs) that have recently been shown to be highly diverse and highly widespread but are still rarely described. IMEs encode their own excision and integration and use the conjugation machinery of unrelated co-resident conjugative element for their own transfer. Recent studies revealed a much more complex and much more diverse lifecycle than initially thought. Besides their main transmission as integrated elements, IMEs probably use plasmid-like strategies to ensure their maintenance after excision. Their interaction with conjugative elements reveals not only harmless hitchhikers but also hunters that use conjugative elements as target for their integration or harmful parasites that subvert the conjugative apparatus of incoming elements to invade cells that harbor them. IMEs carry genes conferring various functions, such as resistance to antibiotics, that can enhance the fitness of their hosts and that contribute to their maintenance in bacterial populations. Taken as a whole, IMEs are probably major contributors to bacterial evolution.

Interconnection of Reactive Oxygen Species Chemistry across the Interfaces of Atmospheric, Environmental, and Biological Processes

Abstract: ConspectusOxidation reactions are ubiquitous and play key roles in the chemistry of the atmosphere, in water treatment processes, and in aerobic organisms. Ozone (O3), hydrogen peroxide (H2O2), hydrogen polyoxides (H2Ox, x > 2), associated hydroxyl and hydroperoxyl radicals (HOx = OH and HO2), and superoxide and ozonide anions (O2– and O3–, respectively) are the primary oxidants in these systems. They are commonly classified as reactive oxygen species (ROS).Atmospheric chemistry is driven by a complex system of chain reactions of species, including nitrogen oxides, hydroxyl and hydroperoxide radicals, alkoxy and peroxy radicals, and ozone. HOx radicals contribute to keeping air clean, but in polluted areas, the ozone concentration increases and creates a negative impact on plants and animals. Indeed, ozone concentration is used to assess air quality worldwide. Clouds have a direct effect on the chemical composition of the atmosphere. On one hand, cloud droplets absorb many trace atmospheric gases, which c...

Stability Analysis of Discrete-Time Infinite-Horizon Optimal Control With Discounted Cost

Abstract: We analyze the stability of general nonlinear discrete-time systems controlled by an optimal sequence of inputs that minimizes an infinite-horizon discounted cost. First, assumptions related to the controllability of the system and its detectability with respect to the stage cost are made. Uniform semiglobal and practical stability of the closed-loop system is then established, where the adjustable parameter is the discount factor. Stronger stability properties are thereupon guaranteed by gradually strengthening the assumptions. Next, we show that the Lyapunov function used to prove stability is continuous under additional conditions, implying that stability has a certain amount of nominal robustness. The presented approach is flexible and we show that robust stability can still be guaranteed when the sequence of inputs applied to the system is no longer optimal but near-optimal. We also analyze stability for cost functions in which the importance of the stage cost increases with time, opposite to discounting. Finally, we exploit stability to derive new relationships between the optimal value functions of the discounted and undiscounted problems, when the latter is well-defined.

Progress in Understanding Low-Temperature Organic Compound Oxidation Using a Jet-Stirred Reactor

Abstract: The jet-stirred reactor (JSR) has become a tool frequently used to study the oxidation of a wide range of reactants and particularly to obtain data for testing detailed kinetic models. This paper aims to discuss recent knowledge pertaining to low-temperature oxidation of hydrocarbons and oxygenated reactants that has been gained from using a JSR in connection with gas chromatography, especially for the detailed quantification of cyclic ethers. Furthermore, JSR in conjunction with mass spectrometry has been applied to the detection of hydroperoxides, including ketohydroperoxides, acids, and compounds with two carbonyl functions. Finally, along with optical diagnostics, JSR has notably been used for the detection of hydrogen peroxide and OH and HO2 radicals. These aspects are also discussed here.