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Showing papers by "University of Los Andes published in 2006"


Journal ArticleDOI
12 Jan 2006-Nature
TL;DR: It is shown that a recent mass extinction associated with pathogen outbreaks is tied to global warming, and it is proposed that temperatures at many highland localities are shifting towards the growth optimum of Batrachochytrium, thus encouraging outbreaks.
Abstract: As the Earth warms, many species are likely to disappear, often because of changing disease dynamics. Here we show that a recent mass extinction associated with pathogen outbreaks is tied to global warming. Seventeen years ago, in the mountains of Costa Rica, the Monteverde harlequin frog (Atelopus sp.) vanished along with the golden toad (Bufo periglenes). An estimated 67% of the 110 or so species of Atelopus, which are endemic to the American tropics, have met the same fate, and a pathogenic chytrid fungus (Batrachochytrium dendrobatidis) is implicated. Analysing the timing of losses in relation to changes in sea surface and air temperatures, we conclude with 'very high confidence' (> 99%, following the Intergovernmental Panel on Climate Change, IPCC) that large-scale warming is a key factor in the disappearances. We propose that temperatures at many highland localities are shifting towards the growth optimum of Batrachochytrium, thus encouraging outbreaks. With climate change promoting infectious disease and eroding biodiversity, the urgency of reducing greenhouse-gas concentrations is now undeniable.

1,528 citations


Journal ArticleDOI
23 Jun 2006-Science
TL;DR: Experimental results from 15 diverse populations show that all populations demonstrate some willingness to administer costly punishment as unequal behavior increases, and the magnitude of this punishment varies substantially across populations, and costly punishment positively covaries with altruistic behavior across populations.
Abstract: Recent behavioral experiments aimed at understanding the evolutionary foundations of human cooperation have suggested that a willingness to engage in costly punishment, even in one-shot situations, may be part of human psychology and a key element in understanding our sociality. However, because most experiments have been confined to students in industrialized societies, generalizations of these insights to the species have necessarily been tentative. Here, experimental results from 15 diverse populations show that (i) all populations demonstrate some willingness to administer costly punishment as unequal behavior increases, (ii) the magnitude of this punishment varies substantially across populations, and (iii) costly punishment positively covaries with altruistic behavior across populations. These findings are consistent with models of the gene-culture coevolution of human altruism and further sharpen what any theory of human cooperation needs to explain.

1,334 citations


Journal ArticleDOI
TL;DR: In this paper, the authors presented a new synthesis and interpolation of the basal area and aboveground live biomass of old-growth lowland tropical forests across South America, based on data from 227 forest plots, many previously unpublished.
Abstract: The biomass of tropical forests plays an important role in the global carbon cycle, both as a dynamic reservoir of carbon, and as a source of carbon dioxide to the atmosphere in areas undergoing deforestation. However, the absolute magnitude and environmental determinants of tropical forest biomass are still poorly understood. Here, we present a new synthesis and interpolation of the basal area and aboveground live biomass of old-growth lowland tropical forests across South America, based on data from 227 forest plots, many previously unpublished. Forest biomass was analyzed in terms of two uncorrelated factors: basal area and mean wood density. Basal area is strongly affected by local landscape factors, but is relatively invariant at regional scale in moist tropical forests, and declines significantly at the dry periphery of the forest zone. Mean wood density is inversely correlated with forest dynamics, being lower in the dynamic forests of western Amazonia and high in the slow-growing forests of eastern Amazonia. The combination of these two factors results in biomass being highest in the moderately seasonal, slow growing forests of central Amazonia and the Guyanas (up to 350?Mg?dry weight?ha?1) and declining to 200-250?Mg?dry weight?ha?1 at the western, southern and eastern margins. Overall, we estimate the total aboveground live biomass of intact Amazonian rainforests (area 5.76 × 106?km2 in 2000) to be 93±23?Pg?C, taking into account lianas and small trees. Including dead biomass and belowground biomass would increase this value by approximately 10% and 21%, respectively, but the spatial variation of these additional terms still needs to be quantified

600 citations


Journal ArticleDOI
15 Jun 2006-Nature
TL;DR: It is shown that a hybrid trait in an animal species can directly cause reproductive isolation, and that the phenotype of H. heurippa reproductively isolates it from both parental species.
Abstract: Speciation is generally regarded to result from the splitting of a single lineage. An alternative is hybrid speciation, considered to be extremely rare, in which two distinct lineages contribute genes to a daughter species. Here we show that a hybrid trait in an animal species can directly cause reproductive isolation. The butterfly species Heliconius heurippa is known to have an intermediate morphology and a hybrid genome, and we have recreated its intermediate wing colour and pattern through laboratory crosses between H. melpomene, H. cydno and their F1 hybrids. We then used mate preference experiments to show that the phenotype of H. heurippa reproductively isolates it from both parental species. There is strong assortative mating between all three species, and in H. heurippa the wing pattern and colour elements derived from H. melpomene and H. cydno are both critical for mate recognition by males.

450 citations


Journal ArticleDOI
V. M. Abazov1, Brad Abbott2, M. Abolins3, Bobby Samir Acharya4  +814 moreInstitutions (74)
TL;DR: The D0 experiment enjoyed a very successful data-collection run at the Fermilab Tevatron collider between 1992 and 1996 as discussed by the authors, and the detector has been upgraded to take advantage of improvements to the Tevoton and to enhance its physics capabilities.
Abstract: The D0 experiment enjoyed a very successful data-collection run at the Fermilab Tevatron collider between 1992 and 1996. Since then, the detector has been upgraded to take advantage of improvements to the Tevatron and to enhance its physics capabilities. We describe the new elements of the detector, including the silicon microstrip tracker, central fiber tracker, solenoidal magnet, preshower detectors, forward muon detector, and forward proton detector. The uranium/liquid-argon calorimeters and central muon detector, remaining from Run I, are discussed briefly. We also present the associated electronics, triggering, and data acquisition systems, along with the design and implementation of software specific to D0.

425 citations


Journal ArticleDOI
TL;DR: In this article, the authors presented a spatial classification of the environmental and ecological diversity of the cerrado region of Brazil, as well as an appraisal of the levels of conversion to agriculture and of the extent of land protection.
Abstract: Aim This paper presents a spatial classification of the environmental and ecological diversity of the cerrado region of Brazil, as well as an appraisal of the levels of conversion to agriculture and of the extent of land protection. Location The cerrado region of Brazil, located in the centre of Brazil, occupies c. 2.5 million square kilometres. Our study area represents roughly 85% of the total. This ecologically heterogeneous region is dominated by savannas, but also contains several types of forests and xerophytic communities that often form mosaics with the savannas. Its high biodiversity is seriously threatened by the accelerated process of conversion to agriculture and a deficiency in the extent and the representativeness of protected areas. Methods We selected 124 land systems from a previous study of the lowlands of South America. The maps were digitized and 41 parameters, where environmental information was available, were used to build a matrix. A cluster analysis was then performed and the results used to classify the land systems into units at two scales. The larger units, characterized by the dominant landform and vegetation, were considered as landscape units. Within each of these, smaller units called ecological units were defined by the physiognomy and phenology of the dominant vegetation, topography and drainage. Using GIS, we mapped the resulting ecological units and incorporated the information on land use from the municipal agricultural census of 1996. In addition, data on the extent of protected land units was used to assess the status of land protection in each ecological unit. Results Five landscape units and 15 ecological units were identified, mapped and explained. These units were not continuous but were represented by disjunct patches located in different parts of the study region. Brief descriptions are given including the geographical locations and dominant ecological features. They also include the extent of land conversion to agriculture (1996 census figures) in each of the various patches as well as the number and area of units of conservation. Main conclusions The high level of land conversion to agriculture is a major threat to the conservation of the remarkable biodiversity of the cerrado region. This, together with the poor status of land protection, represents the major environmental problem facing this region. However, the fact that areas with similar general ecological conditions have a disjunct distribution is important for conservation purposes, even though the details of floristic similarities and biogeographic influences have still to be worked out. Our detailed spatial classification has made this disjunction clearly apparent and has allowed us to map ecologically similar areas accurately. This allows the evaluation of the status of these areas in terms of land use and land protection and may be used in the design of conservation strategies.

315 citations


Journal ArticleDOI
TL;DR: This study provides the first evidence for possible sexual reproduction in P. brasiliensis S1, but does not rule it out in the other two species.
Abstract: Paracoccidioides brasiliensis is the etiologic agent of paracoccidioidomycosis, a disease confined to Latin America and of marked importance in the endemic areas due to its frequency and severity. This species is considered to be clonal according to mycological criteria and has been shown to vary in virulence. To characterize natural genetic variation and reproductive mode in this fungus, we analyzed P. brasiliensis phylogenetically in search of cryptic species and possible recombination using concordance and nondiscordance of gene genealogies with respect to phylogenies of eight regions in five nuclear loci. Our data indicate that this fungus consists of at least three distinct, previously unrecognized species: S1 (species 1 with 38 isolates), PS2 (phylogenetic species 2 with six isolates), and PS3 (phylogenetic species 3 with 21 isolates). Genealogies of four of the regions studied strongly supported the PS2 clade, composed of five Brazilian and one Venezuelan isolate. The second clade, PS3, composed solely of 21 Colombian isolates, was strongly supported by the alpha-tubulin genealogy. The remaining 38 individuals formed S1. Two of the three lineages of P. brasiliensis, S1 and PS2, are sympatric across their range, suggesting barriers to gene flow other than geographic isolation. Our study provides the first evidence for possible sexual reproduction in P. brasiliensis S1, but does not rule it out in the other two species.

303 citations


Journal ArticleDOI
TL;DR: The failure of these mitochondrial genes as well as previous 18S rDNA studies to resolve many of the deeper nodes within the tree suggest that octocorals underwent a rapid radiation and that large amounts of sequence data will be required in order to resolve the basal relationships within the clade.

223 citations


Journal ArticleDOI
TL;DR: The taste of sugar can increase extracellular dopamine in the nucleus accumbens without fail in animals on a dietary regimen that causes bingeing and sugar dependency, and this support the hypothesis that dopamine is released repeatedly in response to taste when bingeing on sweet food.

188 citations


Journal ArticleDOI
TL;DR: Thanks to a co-ordinated programme in the southernmost countries of South America, transmission of T. cruzi by the vectors or blood transfusion has been successfully interrupted in Uruguay, Chile, Chile and Brazil, and the global incidence of new human infection with T.cruzi has decreased by 67%.
Abstract: Human American trypanosomiasis or Chagas disease -- named after Carlos Chagas who first described it in 1909 -- exists only on the American continent. It is caused by a parasite, Trypanosoma cruzi, that is transmitted to humans by blood-sucking triatomine bugs, by blood transfusion, and transplacentally. Chagas disease has two, successive phases: acute and chronic. The acute phase lasts 6-8 weeks. After several years of starting the chronic phase, 20%-35% of infected individuals (the percentage varying with geographical area) develop irreversible lesions of the autonomous nervous system in the heart, the oesophagus, the colon and/or the peripheral nervous system. Data on the prevalence and distribution of Chagas disease markedly improved in quality during the 1980s, as a result of demographically representative, cross-sectional studies carried out in countries where no accurate information on these parameters was available. Experts had previously met in Brasilia, in 1979, and devised standard protocols for carrying out country-wide studies not only on the prevalence of human infection with T. cruzi but also on house infestation with the triatomine vectors. Thanks to a co-ordinated programme in the southernmost countries of South America (i.e.the 'Southern Cone'), transmission of T. cruzi by the vectors or blood transfusion has been successfully interrupted in Uruguay (from 1997), Chile (from 1999) and Brazil (from 2005), and the global incidence of new human infection with T. cruzi has decreased by 67%. Similar multi-country control initiatives have been launched in the Andean countries and in Central America, with the goal of interrupting all transmission of T. cruzi to humans by 2010 -- a goal set, in 1998, as a resolution of the World Health Assembly. Recent advances in basic research on T. cruzi include the genetic characterization of populations of the parasite and the sequencing of its genome.

183 citations


Posted Content
TL;DR: The effects of decentralization on public sector outputs is much debated but little agreed upon as discussed by the authors, and the remarkable case of Bolivia with the more complex case of Colombia to explore decentralization's effects on public education outcomes.
Abstract: The effects of decentralization on public sector outputs is much debated but little agreed upon. This paper compares the remarkable case of Bolivia with the more complex case of Colombia to explore decentralization's effects on public education outcomes. In Colombia, decentralization of education finance improved enrollment rates in public schools. In Bolivia, decentralization made government more responsive by re-directing public investment to areas of greatest need. In both countries, investment shifted from infrastructure to primary social services. In both, it was the behavior of smaller, poorer, more rural municipalities that drove these changes.

Journal ArticleDOI
TL;DR: Evaluating the relationship between sex steroid hormones, sex hormone-binding-globulin, leptin, insulin and insulin resistance in obese men shows that in a sample of men, Tt and SHBG concentrations proportionally diminished with both the increase of BMI and insulin Resistance index.
Abstract: The objective of this work was to evaluate the relationship between sex steroid hormones, sex hormone-binding-globulin, leptin, insulin and insulin resistance in obese men. Anthropometrical indexes...

Journal ArticleDOI
TL;DR: In this article, the authors used panel data from 61 countries at different stages of economic development over a 20-year period to investigate regional differences in the effect of corruption on economic growth and income distribution.
Abstract: This paper uses panel data from 61 countries at different stages of economic development over a 20-year period to investigate regional differences in the effect of corruption on economic growth and income distribution. Using two measures of corruption, we find that there are statistically significant regional differences in the growth and distributional impacts of corruption. The largest growth impact of corruption is found in African countries while OECD and Asian countries have the lowest growth impact. On the other hand, the largest distributional impact of corruption in found in Latin America. A 10% decrease in corruption increases the growth rate of income by about 1.7% in OECD and Asian countries, 2.6% in Latin American countries, and by 2.8% in African countries. A one standard deviation decrease in corruption decreases the gini coefficient of income distribution (0–1 scale) by 0.05 points, 0.14 points, 0.25 points, and 0.33 points in OECD, Asian, African, and Latin American countries, respectively. The results are robust to various specifications, measurement of corruption, measures of investment, as well as the conditioning variables. The results have interesting policy implications for economic growth, especially in low income countries with high rates of corruption.

Journal ArticleDOI
V. M. Abazov1, Brad Abbott2, M. Abolins3, B. S. Acharya4  +593 moreInstitutions (80)
TL;DR: The first direct two-sided bound on the oscillation frequency was reported in this article, where a large sample of semileptonic decays corresponding to approximately 1 fb$^{-1}$ of integrated luminosity collected by the \dzer\ experiment in 2002--2006 during Run II of the Fermilab Tevatron Collider was used.
Abstract: We report the first direct two-sided bound on the $B^0_s$ oscillation frequency using a large sample of $B^0_s$ semileptonic decays corresponding to approximately 1 fb$^{-1}$ of integrated luminosity collected by the \dzer\ experiment in 2002--2006 during Run II of the Fermilab Tevatron Collider. The flavor (i.e., $B^0_s$ or $\bar{B}^0_s$) of the \bs meson at the time of production was found using an opposite-side tagging technique, and the flavor at the time of decay was determined from the charge of the muon in the partially reconstructed decay $\bs\to \mu^{+}D_{s}^{-}X$, $D_{s}^{-}\to \phi \pi^{-}$, $\phi\to K^{+}K^{-}$. A likelihood scan over the oscillation frequency, $\Delta m_s$, gives a most probable value of 19 ps$^{-1}$ and a range of $17 < \Delta m_s < 21$ ps$^{-1}$ at the 90% C.L. At $\Delta m_s=19$ ps$^{-1}$, the amplitude method yields a result that deviates from the hypothesis of an oscillation amplitude of zero by 2.5 standard deviations, corresponding to a two-sided C.L. of 1%.

Journal ArticleDOI
TL;DR: The absence of fluid-ordered/fluid-disordered phase coexistence in the ternary mixtures studied is suggested in contrast to that observed at similar molar concentrations with other ceramide-base-containing lipid mixtures (such as POPC/sphingomyelin/cholesterol, which is used as a canonical raft model membrane).

Journal ArticleDOI
TL;DR: Three new species of Botryosphaeria rhodina are described here as three new species L. venezuelensis sp.
Abstract: Botryosphaeria rhodina (anamorph Lasiodiplodia theobromae) is a common endophyte and opportunistic pathogen on more than 500 tree species in the tropics and subtropics. During routine disease surveys of plantations in Australia and Venezuela several isolates differing from L. theobromae were identified and subsequently characterized based upon morphology and ITS and EF1-alpha nucleotide sequences. These isolates grouped into three strongly supported clades related to but different from the known taxa, B. rhodina and L. gonubiensis, These have been described here as three new species L. venezuelensis sp. nov., L. crassispora sp. nov. and L. rubropurpurea sp. nov. The three could be distinguished easily from each other and the two described species of Lasiodiplodia, thus confirming phylogenetic separations. Furthermore all five Lasiodiplodia spp. now recognized separated from Diplodia spp. and Dothiorella spp. with 100% bootstrap support.

Journal ArticleDOI
TL;DR: In this article, a general phenomenology of the demulsification process is outlined, which is related to the hydrophilicity and concentration of the added demulsifier, and a general formulation of the optimum formulation at which the stability of the emulsion is minimum.
Abstract: Water-in-oil emulsions formed during oil slicks or petroleum production are known to be stabilized by surfactant molecules that naturally occur in the crude oil, e.g., asphaltenes, which are quite lipophilic in nature. Demulsifier substances combine with naturally occurring surfactants to attain a so-called optimum formulation at which the stability of the emulsion is minimum. The attainment of this formulation is related to the hydrophilicity and concentration of the added demulsifier, and a general phenomenology of the demulsification process is outlined.

Journal ArticleDOI
TL;DR: This paper introduces a new approach to statistical moment characterization which is well defined over all processes with algebraic or lighter tails, and derives a ZOS framework for location estimation that gives rise to a novel mode-type estimator with important optimality properties under very impulsive noise.
Abstract: Impulsive or heavy-tailed processes with infinite variance appear naturally in a variety of practical problems that include wireless communications, teletraffic, hydrology, geology, and economics. Most signal processing and statistical methods available in the literature have been designed under the assumption that the processes possess finite variance, and they usually break down in the presence of infinite variance. Although methods based on fractional lower-order statistics (FLOS) have proven successful in dealing with infinite variance processes, they fail in general when the noise distribution has very heavy algebraic tails. In this paper, we introduce a new approach to statistical moment characterization which is well defined over all processes with algebraic or lighter tails. Unlike FLOS, these zero-order statistics (ZOS), as we will call them, provide a common ground for the analysis of basically any distribution of practical use known today. Three new parameters, namely the geometric power, the zero-order location and the zero-order dispersion, constitute the foundation of ZOS. They play roles similar to those played by the power, the expected value and the standard deviation, in the theory of second-order processes. We analyze the properties of the new parameters, and derive a ZOS framework for location estimation that gives rise to a novel mode-type estimator with important optimality properties under very impulsive noise. Several simulations are presented to illustrate the potential of ZOS methods

Journal ArticleDOI
TL;DR: In this article, the authors identify some effects of the global trend towards stronger protection of intellectual property rights on developing countries, and traces related debates, and analyze the consequences of the Bayh-Dole Act and patenting of research tools on international scientific cooperation.

Journal ArticleDOI
TL;DR: In this article, the authors discuss the structure of the light Higgs and the role of the Peccei-Quinn symmetry for dark matter and the predictivity of the theory.
Abstract: We discuss the ordinary, nonsupersymmetric SO(10) as a theory of fermion masses and mixings. We construct two minimal versions of the Yukawa sector based on ${\overline{\mathbf{126}}}_{\mathbf{H}}$ and either ${\mathbf{10}}_{\mathbf{H}}$ or ${\mathbf{120}}_{\mathbf{H}}$. The latter case is of particular interest since it connects the absolute neutrino mass scale with the size of the atmospheric mixing angle ${\ensuremath{\theta}}_{A}$. It also relates the smallness of ${V}_{cb}$ with the largeness of ${\ensuremath{\theta}}_{A}$. These results are based on the analytic study of the second and third generations. Furthermore, we discuss the structure of the light Higgs and the role of the Peccei-Quinn symmetry for dark matter and the predictivity of the theory.

Journal ArticleDOI
TL;DR: In this paper, the importance of hydrology and benthic invertebrates to decomposition of leaves in a tropical stream was assessed using decomposition experiments with leaves of Cecropialatiloba, Tessariaintegrifolia, and Symmeriapaniculata.
Abstract: Decomposition of allochthonous material is a central process in organic matter budgets of low-order streams. Shredding invertebrates contribute substantially to the decomposition of leaves in northern-temperate-zone streams, but their role in tropical streams is less clear. Hydrology has an overarching control on ecological processes in streams, especially on floodplains, where flooding of the mainstem river may cause backflooding in the tributaries. Decomposition experiments with leaves of Cecropialatiloba, Tessariaintegrifolia, and Symmeriapaniculata were used to assess the importance of hydrology and benthic invertebrates to decomposition in a neotropical floodplain stream. Leaf breakdown rates and leaf-associated aquatic invertebrate assemblages were compared among leaf types in a typical 1st-order stream of the Amazonian whitewater floodplain forest near Leticia, Colombia. The stream hydrograph was influenced by regular rainfall throughout the year and by seasonal backflooding from the Amazon...

Journal ArticleDOI
TL;DR: The combined serological, parasitological, epidemiological and molecular data is gathered here to call the attention on the presence of infected dogs as a risk factor in the maintenance of T. cruzi as a source for infection to humans.

Journal ArticleDOI
TL;DR: The hypothesis that variation in the male‐male communication system of the Amazonian frog, Allobates femoralis, is correlated with the occurrence of a single species calling within an overlapping frequency range is tested.
Abstract: The efficacy of communication relies on detection of species-specific signals against the background noise. Features affecting signal detection are thus expected to evolve under selective pressures represented by masking noise. Spectral partitioning between the auditory signals of co-occurring species has been interpreted as the outcome of the selective effects of masking interference. However, masking interference depends not only on signal's frequency but on receiver's range of frequency sensitivity; moreover, selection on signal frequency can be confounded by selection on body size, because these traits are often correlated. To know whether geographic variation in communication traits agrees with predictions about masking interference effects, we tested the hypothesis that variation in the male-male communication system of the Amazonian frog, Allobates femoralis, is correlated with the occurrence of a single species calling within an overlapping frequency range, Epipedobates trivittatus. We studied frogs at eight sites, four where both species co-occur and four where A. femoralis occurs but E. trivittatus does not. To study the sender component of the communication system of A. femoralis and to describe the use of the spectral range, we analyzed the signal's spectral features of all coactive species at each site. To study the receiver component, we derived frequency-response curves from playback experiments conducted on territorial males of A. femoralis under natural conditions. Most geographic variation in studied traits was correlated with either call frequency or with response frequency range. The occurrence of E. trivittatus significantly predicted narrower and asymmetric frequency-response curves in A. femoralis, without concomitant differences in the call or in body size. The number of acoustically coactive species did not significantly predict variation in any of the studied traits. Our results strongly support that the receiver but not the sender component of the communication system changed due to masking interference by a single species.

Journal ArticleDOI
TL;DR: Different types of fields are considered, interpreted as forms of mass media acting on a social system in the context of Axelrod's model for cultural dissemination, which is a nonequilibrium system of locally interacting elements in a lattice with an absorbing order-disorder phase transition.
Abstract: A nonequilibrium system of locally interacting elements in a lattice with an absorbing order-disorder phase transition is studied under the effect of additional interacting fields. These fields are shown to produce interesting effects in the collective behavior of this system. Both for autonomous and external fields, disorder grows in the system when the probability of the elements to interact with the field is increased. There exists a threshold value of this probability beyond which the system is always disordered. The domain of parameters of the ordered regime is larger for nonuniform local fields than for spatially uniform fields. However, the zero field limit is discontinous. In the limit of vanishingly small probability of interaction with the field, autonomous or external fields are able to order a system that would fall in a disordered phase under local interactions of the elements alone. We consider different types of fields which are interpreted as forms of mass media acting on a social system in the context of Axelrod's model for cultural dissemination.

Journal ArticleDOI
TL;DR: This work designed and implemented three different algorithms for the BOUFLP that are able to obtain a good approximation of the Pareto frontier and used the mathematical programming-based algorithm to identify unique tradeoff opportunities for the reconfiguration of the Colombian coffee supply network.
Abstract: The Colombian coffee supply network, managed by the Federacion Nacional de Cafeteros de Colombia (Colombian National Coffee-Growers Federation), requires slimming down operational costs while continuing to provide a high level of service in terms of coverage to its affiliated coffee growers. We model this problem as a biobjective (cost-coverage) uncapacitated facility location problem (BOUFLP). We designed and implemented three different algorithms for the BOUFLP that are able to obtain a good approximation of the Pareto frontier. We designed an algorithm based on the Nondominated Sorting Genetic Algorithm; an algorithm based on the Pareto Archive Evolution Strategy; and an algorithm based on mathematical programming. We developed a random problem generator for testing and comparison using as reference the Colombian coffee supply network with 29 depots and 47 purchasing centers. We compared the algorithms based on the quality of the approximation to the Pareto frontier using a nondominated space metric inspired on Zitzler and Thiele's. We used the mathematical programming-based algorithm to identify unique tradeoff opportunities for the reconfiguration of the Colombian coffee supply network. Finally, we illustrate an extension of the mathematical programming-based algorithm to perform scenario analysis for a set of uncapacitated location problems found in the literature.

Journal ArticleDOI
TL;DR: A PCR and sequencing-based microsatellite marker system that is stable, easy to assay, adaptable to large series of isolates, and discriminatory enough to be used as a typing system in identifying the three proposed species of P. brasiliensis is described.
Abstract: Paracoccidioides brasiliensis is the etiological agent of paracoccidioidomycosis, an important human systemic mycosis in Latin America. Recently, the existence of three different phylogenetic species (S1, PS2, and PS3) of P. brasiliensis was demonstrated. Despite being genetically isolated, all three species were capable of inducing disease in both humans and animals, although lower virulence has been found with the PS2 species. The available molecular methods developed to characterize and type strains have not been useful for assigning isolates to the described species, creating the need for molecular markers capable of distinguishing genetically isolated groups. Here, we describe a PCR and sequencing-based microsatellite marker system that is stable, easy to assay, adaptable to large series of isolates, and discriminatory enough to be used as a typing system in identifying the three proposed species of P. brasiliensis. In addition, this system provides an unambiguous tool for strain discrimination between two (S1 and PS2) of the three phylogenetic species.

Journal ArticleDOI
TL;DR: This study did not disclose significant polymorphism to separate West African and South American isolates into different species/subspecies and indicate that the complexity of T. vivax in Africa and of the whole subgenus Trypanosoma (Duttonella) might be higher than previously believed.
Abstract: The taxonomic and phylogenetic relationships of Trypanosoma vivax are controversial. It is generally suggested that South American, and East and West African isolates could be classified as subspecies or species allied to T. vivax. This is the first phylogenetic study to compare South American isolates (Brazil and Venezuela) with West/East African T. vivax isolates. Phylogeny using ribosomal sequences positioned all T. vivax isolates tightly together on the periphery of the clade containing all Salivarian trypanosomes. The same branching of isolates within T. vivax clade was observed in all inferred phylogenies using different data sets of sequences (SSU, SSU plus 5.8S or whole ITS rDNA). T. vivax from Brazil, Venezuela and West Africa (Nigeria) were closely related corroborating the West African origin of South American T. vivax, whereas a large genetic distance separated these isolates from the East African isolate (Kenya) analysed. Brazilian isolates from cattle asymptomatic or showing distinct pathology were highly homogeneous. This study did not disclose significant polymorphism to separate West African and South American isolates into different species/subspecies and indicate that the complexity of T. vivax in Africa and of the whole subgenus Trypanosoma (Duttonella) might be higher than previously believed.

Journal ArticleDOI
28 Feb 2006-Primates
TL;DR: It is found that woolly monkeys tend to avoid open-degraded forests, where fruit production is generally lower than it is in mature forests, and differences in activity budgets and the diet preferences of different age/sex classes are found.
Abstract: In this study, I revise three aspects of the socioecology of woolly monkeys (genus Lagothrix) that might give us a better understanding of the patterns found in this species: (1) the association between temporal variation in fruit abundance and diet, activity, and ranging patterns; (2) the individual trade-offs associated with living in small or large groups, and (3) the relationship between social dominance and foraging success. Using behavioral and ecological data collected during 3 years in Tinigua Park, Colombia, I found that woolly monkeys tend to avoid open-degraded forests, where fruit production is generally lower than it is in mature forests. Diet and activity budgets were highly associated with temporal patterns of fruit production. Daily path length was positively correlated with group size and monthly fruit abundance, and negatively correlated with habitat quality. I found differences in activity budgets and the diet preferences of different age/sex classes. For example, adult males rest more and juveniles play more than other classes. Juveniles and adult females without infants look for arthropods more often than adult males and females with young infants, who showed the highest frequencies of fruit feeding. Dominant adult males were not consistently the most efficient foragers on fruits according to two different indexes. Most of these results are consistent with the expectations from strong intra-group competition for resources. However, females with infants received benefits during feeding similar to those of dominant adult males, which may be mediated by differential aggression from males to other group members (juveniles and females without infants).

Journal ArticleDOI
TL;DR: This paper reviewed the theoretical and empirical research on this issue and showed that one of the channels whereby better institutions may have an effect on economic development is through the consolidation of larger and better financial markets.
Abstract: . Among the fundamental causes of long-run economic performance, differences in ‘institutions’ have received considerable attention in recent years. At the same time, a large body of theoretical and empirical work shows that financial development can have a big effect on economic performance. This raises the more fundamental question as to why some countries have developed financial markets while others do not. This paper reviews the theoretical and empirical research on this issue and shows that one of the channels whereby better institutions may have an effect on economic development is through the consolidation of larger and better financial markets. An issue that is left aside in this paper relates to what regulations and policies lead to better functioning capital markets. At some level, one can think of regulations and policies as particular types of institutions. Nonetheless, institutional problems are deeper causes leading to poor economic performance; bad policies might simply be part of the channels through which they influence performance. Thus, addressing the question of what determines the emergence of ‘good’ institutions – i.e. institutions that promote financial development – seems particularly important. Recent research providing some answers to this question is also reviewed.

Journal ArticleDOI
TL;DR: In this paper, an experimental investigation aimed at evaluating the adequacy of the strength factors for concrete struts in strut-and-tie models given in Appendix A of the 2002 ACI Building Code is presented.
Abstract: Results from an experimental investigation aimed at evaluating the adequacy of the strength factors for concrete struts in strut-and-tie models given in Appendix A of the 2002 ACI Building Code are presented. The main design variables considered were: the angle between primary strut-and-tie axes, amount of reinforcement crossing the strut, and concrete strength. A total of 12 deep beams were tested, eight with normal strength concrete and four with high-strength concrete. The ratio between experimentally obtained failure loads and the strengths predicted using the strut strength factors given in Appendix A of the ACI Code ranged between 1.00 and 1.22, and between 0.91 and 1.02 for normal and high-strength concrete beams, respectively. Inconsistencies were found in the provisions for minimum reinforcement crossing a strut in Sections A.3.3 and A.3.3.1 when applied to the test specimens, with the former leading to substantially larger reinforcement ratios. The use of a strut strength factor β s = 0.60 in high-strength concrete bottle-shaped struts without web reinforcement led to strength predictions approximately 10% higher than the experimental failure loads. The limited test results suggest that, as a minimum, an effective reinforcement ratio of 0.01, calculated according to ACI Code, Section A.3.3.1, should be provided in high-strength concrete members when a strength factor β S = 0.60 is used. Additional test data, however, are required before a definite recommendation can be made in this regard.