University of Madras

About: University of Madras is a education organization based out in Chennai, Tamil Nadu, India. It is known for research contribution in the topics: Ring (chemistry) & Lipid peroxidation. The organization has 8496 authors who have published 11369 publications receiving 211152 citations. The organization is also known as: Madras University & University of Chennai.

Canonical Wnt pathway gene expression and their clinical correlation in oral squamous cell carcinoma.

Abstract: Aim: The aim of this study is to explore the prognostic significance and clinicopathological correlations of the Wnt pathway genes in a cohort of surgically treated patients with oral squamous cell carcinoma (OSCC) patients. Settings and Design: A prospective genetic study on patients with OSCC was carried out during the period from July 2014 to January 2016. Informed consent from patients and institutional ethical approval for the study was obtained and the guidelines were strictly followed for collection of samples. Subjects and Methods: Clinical data and mRNA expression analysis of ten genes in the canonical Wnt pathway were evaluated and their relationships with clinical and demographic variables were studied in 58 tissue samples. Wnt-3a, β-catenin, secreted frizzled-related proteins sFRP-1, sFRP-2, sFRP-4, sFRP-5, Wnt inhibitory factor 1, dickkopf-1, c-MYC, and cyclin-D1 from cancer (n = 29) and normal (n = 29) tissue samples were investigated using quantitative reverse transcription-polymerase chain reaction. Statistical Analysis: Descriptive statistics were used to summarize the sample characteristics and clinical variables. If the data were normal, then parametric tests were used; otherwise, nonparametric alternatives were used. All the analyses were carried out using SPSS version 23.0 (IBM SPSS Inc., USA). Results: Expression of sFRP-1, sFRP-2, and sFRP-5 in control samples and expression of c-MYC and cyclin D1 in cancer samples showed statistical significance. Significant expression of Wnt3A was observed among patients who had recurrence and were deceased. Conclusion: Wnt3A, β-catenin, and cyclin D1 are recognized as key components of Wnt/β-catenin signaling. However, in this study, there was no significant expression of all the three genes in OSCC. The proto-oncogene c-MYC showed statistically significant upregulation in cancer tissue samples suggesting that the OSCC among South Indian population is primarily not mediated by the canonical Wnt signaling pathway.

Direct amperometric determination of l-ascorbic acid (Vitamin C) at octacyanomolybdate-doped-poly(4-vinylpyridine) modified electrode in fruit juice and pharmaceuticals

Abstract: The glassy carbon electrode (GCE) modified with Mo(CN) 8 4− -incorporated-poly(4-vinylpyridine) (PVP/Mo(CN) 8 4− ), which has been recently shown to possess several attractive attributes as an efficient electrocatalytic electrode for l -ascorbic acid oxidation and its estimation, is used for l -ascorbic acid estimation directly in orange fruit juice and Celin tablet in a 0.1 M H 2 SO 4 acid solution without any special treatment. Constant potential amperometry at 570 mV (saturated calomel electrode, SCE) in stirred solutions is used for this purpose. A good correlation is attained with the official titrametric method. To understand the possible electrocatalytic reaction mechanism for the electro-oxidation of l -ascorbic acid, calibration graphs over the range 1 × 10 −5 to 1 × 10 −2 mol dm −3 l -ascorbic acid are compared for the three electrodes, ca. PVP/Mo(CN) 8 4− , undoped PVP, and GCE; the curvature at high ascorbic acid concentration for the PVP/Mo(CN) 8 4− electrode is explained in terms of Michaelis–Menten (MM) saturation kinetics. The apparent MM constant ( K M ), the maximum catalytic current ( i M ), the complex decomposition rate constant ( k c ), and the heterogeneous modified electrode rate constant ( k ′ ME ) are calculated from three different approaches. A reasonably high value of ≈1 × 10 −2 cm s −1 is obtained for k ′ ME , indicating efficient l -ascorbic acid mediation at the PVP/Mo(CN) 8 4− electrode, thus accounting for quite a high sensitivity of this modified film electrode compared to several other modified electrodes.