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Institution

University of Modena and Reggio Emilia

EducationModena, Italy
About: University of Modena and Reggio Emilia is a education organization based out in Modena, Italy. It is known for research contribution in the topics: Population & Transplantation. The organization has 8179 authors who have published 22418 publications receiving 671337 citations. The organization is also known as: Università degli Studi di Modena e Reggio Emilia & Universita degli Studi di Modena e Reggio Emilia.


Papers
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Journal ArticleDOI
08 Nov 2017-Nature
TL;DR: It is shown that autologous transgenic keratinocyte cultures regenerated an entire, fully functional epidermis on a seven-year-old child suffering from a devastating, life-threatening form of JEB, providing a blueprint that can be applied to other stem cell-mediated combined ex vivo cell and gene therapies.
Abstract: Junctional epidermolysis bullosa (JEB) is a severe and often lethal genetic disease caused by mutations in genes encoding the basement membrane component laminin-332. Surviving patients with JEB develop chronic wounds to the skin and mucosa, which impair their quality of life and lead to skin cancer. Here we show that autologous transgenic keratinocyte cultures regenerated an entire, fully functional epidermis on a seven-year-old child suffering from a devastating, life-threatening form of JEB. The proviral integration pattern was maintained in vivo and epidermal renewal did not cause any clonal selection. Clonal tracing showed that the human epidermis is sustained not by equipotent progenitors, but by a limited number of long-lived stem cells, detected as holoclones, that can extensively self-renew in vitro and in vivo and produce progenitors that replenish terminally differentiated keratinocytes. This study provides a blueprint that can be applied to other stem cell-mediated combined ex vivo cell and gene therapies. Autologous transgenic epidermal stem cell cultures are used to reconstitute almost the entire epidermis of a patient with severe junctional epidermolysis bullosa. Patients with junctional epidermolysis bullosa (JEB) carry mutations in genes that encode components of the basement membrane, which ensures the integrity between the epidermis and the dermis, such as laminin-332. These mutations cause blistering of the skin and chronic wounds. Following initial treatment of an adult patient with a limited affected region, Michele De Luca and colleagues reconstruct the full epidermis of a 7-year-old patient with autologous transgenic cells transduced with a virus vector carrying the non-mutated form of laminin-322. The integration sites of the virus used for gene delivery provide a tracing tool ex vivo and in vivo and demonstrate that the human epidermis is sustained by a limited number of long-lived stem cells.

506 citations

Journal ArticleDOI
TL;DR: Zhang et al. as mentioned in this paper proposed a convolutional long short-term memory (LSTM) network to iteratively refine the predicted saliency map by focusing on the most salient regions of the input image.
Abstract: Data-driven saliency has recently gained a lot of attention thanks to the use of convolutional neural networks for predicting gaze fixations. In this paper, we go beyond standard approaches to saliency prediction, in which gaze maps are computed with a feed-forward network, and present a novel model which can predict accurate saliency maps by incorporating neural attentive mechanisms. The core of our solution is a convolutional long short-term memory that focuses on the most salient regions of the input image to iteratively refine the predicted saliency map. In addition, to tackle the center bias typical of human eye fixations, our model can learn a set of prior maps generated with Gaussian functions. We show, through an extensive evaluation, that the proposed architecture outperforms the current state-of-the-art on public saliency prediction datasets. We further study the contribution of each key component to demonstrate their robustness on different scenarios.

503 citations

Journal ArticleDOI
TL;DR: This work has shown monounsaturated oleic acid to be less toxic than palmitic acid and to prevent/attenuate palMITic acid hepatocites toxicity in steatosis models in vitro, but to what degree these effects are mediated by Steatosis extent is unknown.
Abstract: Background and Aim: Studies have shown monounsaturated oleic acid to be less toxic than palmitic acid and to prevent/attenuate palmitic acid hepatocites toxicity in steatosis models in vitro. However, to what degree these effects are mediated by steatosis extent is unknown. Methods: We evaluated whether steatosis per se is associated with hepatocytes apoptosis and determined the role of oleic and palmitic acid, the most abundant fatty acids in western diets, on triglyceride accumulation and apoptosis in an in vitro model of steatosis induced in three hepatocytic cell lines (HepG2, HuH7, WRL68). The impact of incubation for 24 h with oleic (0.66 and 1.32 mM) and palmitic acid (0.33 and 0.66 mM), alone or combined (molar ratio 2 : 1) on steatosis, apoptosis, and insulin signalling, was evaluated. Results: Concurrent with PPARγ and SREBP-1 gene activation, steatosis extent was larger when cells were treated with oleic than with palmitic acid; the latter fatty acid was associated with increased PPARα expression. Cell apoptosis was inversely proportional to steatosis deposition. Moreover, palmitic, but not oleic acid, impaired insulin signalling. Despite the higher amount of fat resulting from incubation of the two fatty acids combined, the apoptosis rate and impaired insulin signalling were lower than in cells treated with palmitic acid alone, indicating a protective effect of oleic acid. Conclusions: Oleic acid is more steatogenic but less apoptotic than palmitic acid in hepatocityc cell cultures. These data may provide a biological basis for clinical findings on dietary patterns and pathogenetic models of nonalcoholic fatty liver disease.

502 citations

Journal ArticleDOI
TL;DR: Patients with nonalcoholic fatty liver disease diagnosed by either serum liver enzymes (aminotransferases or gamma‐glutamyltransferase [GGT]) or ultrasonography are gauged for the risk of developing type 2 diabetes and metabolic syndrome.
Abstract: Background and Aim: The magnitude of the risk of incident type 2 diabetes (T2D) and metabolic syndrome (MetS) among patients with nonalcoholic fatty liver disease (NAFLD) is poorly known. We gauged the risk of developing T2D and MetS in patients with NAFLD diagnosed by either serum liver enzymes (aminotransferases or gamma-glutamyltransferase [GGT]) or ultrasonography. Methods: Pertinent prospective studies were identified through extensive electronic database research, and studies fulfilling enrolment criteria were included in the meta-analysis. Results Overall, in a pooled population of 117020 patients (from 20 studies), who were followed-up for a median period of 5 years (range: 3–14.7 years), NAFLD was associated with an increased risk of incident T2D with a pooled relative risk of 1.97 (95% confidence interval [CI], 1.80–2.15) for alanine aminotransferase, 1.58 (95% CI, 1.43–1.74) for aspartate aminotransferase, 1.86 (95% CI, 1.71–2.03) for GGT (last vs first quartile or quintile), and 1.86 (95% CI, 1.76–1.95) for ultrasonography, respectively. Overall, in a pooled population of 81411 patients (from eight studies) who were followed-up for a median period of 4.5 years (range: 3–11 years), NAFLD was associated with an increased risk of incident MetS with a pooled relative risk of 1.80 (95% CI, 1.72–1.89) for alanine aminotransferase (last vs first quartile or quintile), 1.98 (95% CI, 1.89–2.07) for GGT, and 3.22 (95% CI, 3.05–3.41) for ultrasonography, respectively. Conclusions: Nonalcoholic fatty liver disease, as diagnosed by either liver enzymes or ultrasonography, significantly increases the risk of incident T2D and MetS over a median 5-year follow-up.

500 citations

Journal ArticleDOI
TL;DR: The first results of the use of health services for mental disorders in six European countries as part of the ESEMeD project are presented.
Abstract: The ESEMeD ⁄ MHEDEA 2000 investigators. Use of mental health services in Europe: results from the European Study of the Epidemiology of Mental Disorders (ESEMeD) project. Acta Psychiatr Scand 2004: 109 (Suppl. 420): 47–54. a Blackwell Munksgaard 2004. Objective: Comprehensive information about access and patterns of use of mental health services in Europe is lacking. We present the first results of the use of health services for mental disorders in six European countries as part of the ESEMeD project. Method: The study was conducted in: Belgium, France, Germany, Italy, the Netherlands and Spain. Individuals aged 18 years and over who were not institutionalized were eligible for an computer-assisted interview done at home. The 21 425 participants were asked to report how frequently they consulted formal health services due to their emotions or mental health, the type of professional they consulted and the treatment they received as a result of their consultation in the previous year. Results: An average of 6.4% of the total sample had consulted formal health services in the previous 12months. Of the participants with a 12-month mental disorder, 25.7% had consulted a formal health service during that period. This proportion was higher for individuals with a mood disorder (36.5%, 95% CI 32.5–40.5) than for those with anxiety disorders (26.1%, 95% CI 23.1–29.1). Among individuals with a 12-month mental disorder who had contacted the health services 12months previously, approximately two-thirds had contacted a mental health professional. Among those with a 12-month mental disorder consulting formal health services, 21.2% received no treatment. Conclusion: The ESEMeD results suggest that the use of health services is limited among individuals with mental disorders in the European countries studied. The factors associated with this limited access and their implications deserve further research. The ESEMeD ⁄ MHEDEA 2000

496 citations


Authors

Showing all 8322 results

NameH-indexPapersCitations
Carlo M. Croce1981135189007
Gregory Y.H. Lip1693159171742
Geoffrey Burnstock141148899525
Peter M. Rothwell13477967382
Claudio Franceschi12085659868
Lorenzo Galluzzi11847771436
Leonardo M. Fabbri10956660838
David N. Reinhoudt107108248814
Stefano Pileri10063543369
Andrea Bizzeti99116846880
Brian K. Shoichet9828140313
Dante Gatteschi9772748729
Roberta Sessoli9542441458
Thomas A. Buchholz9349433409
Pier Luigi Zinzani9285735476
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Performance
Metrics
No. of papers from the Institution in previous years
YearPapers
202376
2022230
20212,354
20202,083
20191,633
20181,450