University of Mons

About: University of Mons is a education organization based out in Mons, Belgium. It is known for research contribution in the topics: Large Hadron Collider & Standard Model. The organization has 3073 authors who have published 9465 publications receiving 294776 citations.

Peptidic targeting of phosphatidylserine for the MRI detection of apoptosis in atherosclerotic plaques

Abstract: Molecular and cellular imaging of atherosclerosis has garnered more interest at the beginning of the 21st century, with aims to image in vivo biological properties of plaque lesions. Apoptosis seems an attractive target for the diagnosis of vulnerable atherosclerotic plaques prone to a thrombotic event. The aim of the present work was to screen for apoptosis peptide binders by phage display with the final purpose to detect apoptotic cells in atherosclerotic plaques by magnetic resonance imaging (MRI). A phosphatidylserine-specific peptide identified by phage display was thus used to design an MRI contrast agent (CA), which was evaluated as a potential in vivo reporter of apoptotic cells. A library of linear 6-mer random peptides was screened in vitro against immobilized phosphatidylserine. Phage DNA was isolated and sequenced, and the affinity of peptides for phosphatidylserine was evaluated by enzyme-linked immunosorbent assay. The phosphatidylserine-specific peptide and its scrambled homologue were attached to a linker and conjugated to DTPA-isothiocyanate. The products were purified by dialysis and by column chromatography and complexed with gadolinium chloride. After their evaluation using apoptotic cells and a mouse model of liver apoptosis, the phosphatidylserine-targeted CA was used to image atherosclerotic lesions on ApoE(-/-) transgenic mice. Apoptotic cells were detected on liver and aorta specimens by the immunostaining of phosphatidylserine and of active caspase-3. Sequencing of the phage genome highlighted nine different peptides. Their alignment with amino acid sequences of relevant proteins revealed a frequent homology with Ca2+ channels, reminiscent of the function of annexins. Alignment with molecules involved in apoptosis provides a direct correlation between peptide selection and utility. The in vivo MRI studies performed at 4.7 T provide proof of concept that apoptosis-related pathologies could be diagnosed by MRI with a low molecular weight paramagnetic agent. The new CA could have real potential in the diagnosis and therapy monitoring of atherosclerotic disease and of other apoptosis-associated pathologies, such as cancer, ischemia, chronic inflammation, autoimmune disorders, transplant rejection, neurodegenerative disorders, and diabetes mellitus. The phage display-derived peptide could also play a potential therapeutic role through anticoagulant activity by mimicking the role of annexin V, the endogenous ligand of phosphatidylserine.

Search for pair production of first- and second-generation scalar leptoquarks in pp collisions at √s=7TeV

Abstract: Results are presented from a search for the pair production of first- and second-generation scalar leptoquarks in proton-proton collisions at sqrt(s)= 7 TeV. The data sample corresponds to an integrated luminosity of 5.0 inverse femtobarns, collected by the CMS detector at the LHC. The search signatures involve either two charged leptons of the same-flavour (electrons or muons) and at least two jets, or a single charged lepton (electron or muon), missing transverse energy, and at least two jets. If the branching fraction of the leptoquark decay into a charged lepton and a quark is assumed to be beta=1, leptoquark pair production is excluded at the 95% confidence level for masses below 830 GeV and 840 GeV for the first and second generations, respectively. For beta = 0.5, masses below 640 GeV and 650 GeV are excluded. These limits are the most stringent to date.

Multiple dark matter scenarios from ubiquitous stringy throats

Abstract: We discuss the possibility of having multiple Kaluza-Klein (KK) dark matter candidates which arise naturally in generic Type-IIB string theory compactification scenarios. These dark matter candidates reside in various throats of the Calabi-Yau manifold. In principle, they can come with varied range of masses in four-dimensions depending upon the hierarchical warping of the throats. We show that consistency with cosmological bounds and four-dimensional effective theory description imposes strong constraints on the parameter space and the geometry of the throats. With a rather model-independent approach, we find that the mass scales allowed for the KK dark matter particles in various throats can vary between 0.1 eV and 10 TeV, depending upon the throat geometry. Thus, there could be simultaneously more than one kind of cold (and possibly warm and hot) dark matter components residing in the Universe. This multiple dark matter scenario could weaken the bound on a conventional supersymmetric dark matter candidate and could also account for extra relativistic degrees of freedom in our Universe.

Constraints on parton distribution functions and extraction of the strong coupling constant from the inclusive jet cross section in pp collisions at √s = 7 TeV

Abstract: The inclusive jet cross section for proton-proton collisions at a centre-of-mass energy of 7 TeV was measured by the CMS Collaboration at the LHC with data corresponding to an integrated luminosity of 5.0 inverse femtobarns. The measurement covers a phase space up to 2 TeV in jet transverse momentum and 2.5 in absolute jet rapidity. The statistical precision of these data leads to stringent constraints on the parton distribution functions of the proton. The data provide important input for the gluon density at high fractions of the proton momentum and for the strong coupling constant at large energy scales. Using predictions from perturbative quantum chromodynamics at next-to-leading order, complemented with electroweak corrections, the constraining power of these data is investigated and the strong coupling constant at the Z boson mass M[Z] is determined to be alpha[S(M[Z])} = 0.1185 +/- 0.0019 (exp) +0.0060 -0.0037 (theo), which is in agreement with the world average.