Showing papers by "University of Montpellier published in 2005"

Phenotypic and genetic differentiation between native and introduced plant populations

Abstract: Plant invasions often involve rapid evolutionary change. Founder effects, hybridization, and adaptation to novel environments cause genetic differentiation between native and introduced populations and may contribute to the success of invaders. An influential idea in this context has been the Evolution of Increased Competitive Ability (EICA) hypothesis. It proposes that after enemy release plants rapidly evolve to be less defended but more competitive, thereby increasing plant vigour in introduced populations. To detect evolutionary change in invaders, comparative studies of native versus introduced populations are needed. Here, we review the current empirical evidence from: (1) comparisons of phenotypic variation in natural populations; (2) comparisons of molecular variation with neutral genetic markers; (3) comparisons of quantitative genetic variation in a common environment; and (4) comparisons of phenotypic plasticity across different environments. Field data suggest that increased vigour and reduced herbivory are common in introduced plant populations. In molecular studies, the genetic diversity of introduced populations was not consistently different from that of native populations. Multiple introductions of invasive plants appear to be the rule rather than the exception. In tests of the EICA hypothesis in a common environment, several found increased growth or decreased resistance in introduced populations. However, few provided a full test of the EICA hypothesis by addressing growth and defence in the same species. Overall, there is reasonable empirical evidence to suggest that genetic differentiation through rapid evolutionary change is important in plant invasions. We discuss conceptual and methodological issues associated with cross-continental comparisons and make recommendations for future research. When testing for EICA, greater emphasis should be put on competitive ability and plant tolerance. Moreover, it is important to address evolutionary change in characteristics other than defence and growth that could play a role in plant invasions.

Mobilization of vacuolar iron by AtNRAMP3 and AtNRAMP4 is essential for seed germination on low iron

Abstract: Iron (Fe) is necessary for all living cells, but its bioavailability is often limited. Fe deficiency limits agriculture in many areas and affects over a billion human beings worldwide. In mammals, NRAMP2/DMT1/DCT1 was identified as a major pathway for Fe acquisition and recycling. In plants, AtNRAMP3 and AtNRAMP4 are induced under Fe deficiency. The similitude of AtNRAMP3 and AtNRAMP4 expression patterns and their common targeting to the vacuole, together with the lack of obvious phenotype in nramp3-1 and nramp4-1 single knockout mutants, suggested a functional redundancy. Indeed, the germination of nramp3 nramp4 double mutants is arrested under low Fe nutrition and fully rescued by high Fe supply. Mutant seeds have wild type Fe content, but fail to retrieve Fe from the vacuolar globoids. Our work thus identifies for the first time the vacuole as an essential compartment for Fe storage in seeds. Our data indicate that mobilization of vacuolar Fe stores by AtNRAMP3 and AtNRAMP4 is crucial to support Arabidopsis early development until efficient systems for Fe acquisition from the soil take over.

From the discovery of the Malta fever's agent to the discovery of a marine mammal reservoir, brucellosis has continuously been a re-emerging zoonosis

Abstract: Brucellosis is not a sustainable disease in humans. The source of human infection always resides in domestic or wild animal reservoirs. The routes of infection are multiple: food-borne, occupational or recreational, linked to travel and even to bioterrorism. New Brucella strains or species may emerge and existing Brucella species adapt to changing social, cultural, travel and agricultural environment. Brucella melitensis is the most important zoonotic agent, followed by Brucella abortus and Brucella suis. This correlates with the fact that worldwide, the control of bovine brucellosis (due to B. abortus) has been achieved to a greater extent than the control of sheep and goat brucellosis (due to B. melitensis), these latter species being the most important domestic animals in many developing countries. The long duration and high cost of treatment of human brucellosis reduces the efficacy of the therapy. There is no human vaccine for brucellosis and the occurrence of brucellosis is directly linked to the status of animal brucellosis in a region. In this context, the Word Health Organization has defined the development of a human vaccine, besides the implementation of control and eradication programs in animals, as a high priority. The pathogenicity for humans of B. suis biovars 1, 3 and 4 is well established, whereas B. suis biovar 2 seems to be less pathogenic. Indeed, although hunters and pig farmers have repeatably experienced infectious contact with B. suis biovar 2 (found in wild boar and outdoor-rearing pigs in Europe), isolation of B. suis biovar 2 from human samples have only been seldom reported. Marine mammal brucellosis, due to two new proposed Brucella species i.e. B. cetaceae and B. pinnipediae, represents a new zoonotic threat but the pathogenicity for humans of the different Brucella species found in cetaceans and pinnipeds still has to be clearly established.

The effective temperature scale of galactic red supergiants : Cool, but not as cool as we thought

Abstract: We use moderate-resolution optical spectrophotometry and the new MARCS stellar atmosphere models to determine the effective temperatures of 74 Galactic red supergiants (RSGs). The stars are mostly members of OB associations or clusters with known distances, allowing a critical comparison with modern stellar evolutionary tracks. We find we can achieve excellent matches between the observations and the reddened model fluxes and molecular transitions, although the atomic lines Ca I ?4226 and Ca II H and K are found to be unrealistically strong in the models. Our new effective temperature scale is significantly warmer than those in the literature, with the differences amounting to 400 K for the latest type M supergiants (i.e., M5 I). We show that the newly derived temperatures and bolometric corrections give much better agreement with stellar evolutionary tracks. This agreement provides a completely independent verification of our new temperature scale. The combination of effective temperature and bolometric luminosities allows us to calculate stellar radii; the coolest and most luminous stars (KW Sgr, Case 75, KY Cyg, HD 206936=? Cep) have radii of roughly 1500 Rsolar (7 AU), in excellent accordance with the largest stellar radii predicted from current evolutionary theory, although smaller than that found by others for the binary VV Cep and for the peculiar star VY CMa. We find that similar results are obtained for the effective temperatures and bolometric luminosities using only the dereddened V-K colors, providing a powerful demonstration of the self-consistency of the MARCS models.

Hair cell synaptic ribbons are essential for synchronous auditory signalling

Abstract: Hearing relies on faithful synaptic transmission at the ribbon synapse of cochlear inner hair cells (IHCs). At present, the function of presynaptic ribbons at these synapses is still largely unknown. Here we show that anchoring of IHC ribbons is impaired in mouse mutants for the presynaptic scaffolding protein Bassoon. The lack of active-zone-anchored synaptic ribbons reduced the presynaptic readily releasable vesicle pool, and impaired synchronous auditory signalling as revealed by recordings of exocytic IHC capacitance changes and sound-evoked activation of spiral ganglion neurons. Both exocytosis of the hair cell releasable vesicle pool and the number of synchronously activated spiral ganglion neurons co-varied with the number of anchored ribbons during development. Interestingly, ribbon-deficient IHCs were still capable of sustained exocytosis with normal Ca2+-dependence. Endocytic membrane retrieval was intact, but an accumulation of tubular and cisternal membrane profiles was observed in ribbon-deficient IHCs. We conclude that ribbon-dependent synchronous release of multiple vesicles at the hair cell afferent synapse is essential for normal hearing.

Continuous peripheral nerve blocks in hospital wards after orthopedic surgery: a multicenter prospective analysis of the quality of postoperative analgesia and complications in 1,416 patients

Abstract: Background:Continuous peripheral nerve block (CPNB) is the technique of choice for postoperative analgesia after painful orthopedic surgery. However, the incidence of neurologic and infectious adverse events in the postoperative period are not well established. This issue was the aim of the study.Me

On the relationships between slab dip, back‐arc stress, upper plate absolute motion, and crustal nature in subduction zones

Abstract: [1] Statistical analysis of modern oceanic subduction zone parameters, such as the age of a downgoing plate or the absolute plate motions, is performed in order to investigate which parameter controls the dip of a slab and, conversely, what the influence of slab geometry is on upper plate behavior. For that purpose, parameters have been determined from global databases along 159 transects from all subduction zones that are not perturbed by nearby collision or ridge/plateau/seamount subduction. On the basis of tomographic images, slabs that penetrate through, or lie on, the 670 km discontinuity are also identified. The results of the statistical analysis are as follows: (1) Back-arc stress correlates with slab dip, i.e., back-arc spreading is observed for deep dips (deeper than 125 km) larger than 50°, whereas back-arc shortening occurs only for deep dips less than 30°. (2) Slab dip correlates with absolute motion of the overriding plate. The correlation is even better when the slab lies on, or even more penetrates through, the 670 km discontinuity. (3) Slabs dip more steeply, by about 20° on average, beneath oceanic overriding plates than beneath continental ones. (4) Slabs dip more steeply on average by about 10° near edges. (5) Slab dip does not correlate with the magnitude of slab pull, the age of subducting lithosphere at the trench, the thermal regime of the subducting lithosphere, the convergence rate, or the subduction polarity (east versus west). The present study provides evidence that the upper plate absolute motion plays an important role on slab dip, as well as on upper plate strain. Retreating overriding plates are often oceanic ones and thus may partially explain the steeper slab dips beneath oceanic upper plates. One can infer that low slab dips correlate well with compression in continental advancing upper plates, whereas steep dips are often associated with extension in oceanic retreating upper plates. Excess weight of old slabs is often counterbalanced by other forces, probably asthenospheric in origin, such as lateral mantle flow near slab edges or anchor forces, to determine slab dip.

First stars VI - Abundances of C, N, O, Li, and mixing in extremely metal-poor giants. Galactic evolution of the light elements

Abstract: We have investigated the poorly-understood origin of nitrogen in the early Galaxy by determining N abundances from the NH band at 336 nm in 35 extremely metal-poor halo giants, with carbon and oxygen abundances from Cayrel et al. (\cite{CDS04}, AA these stars are generally more evolved and located on the upper Red Giant Branch (RGB) or Horizontal Branch (HB). The second group has [N/Fe] < 0.5, shows no evidence for C to N conversion, and Li is only moderately diluted; these stars belong to the lower RGB and we conclude that their C and N abundances are very close to those of the gas from which they formed in the early Galaxy, they are called ``unmixed stars''. The [O/Fe] and [(C+N)/Fe] ratios are the same in the two groups, confirming that the differences between them are caused by dredge-up of CN-processed material in the first group, with negligible contributions from the O-N cycle. The ``unmixed'' stars reflect the abundances in the early Galaxy: the [C/Fe] ratio is constant (about +0.2 dex) and the [C/Mg] ratio is close to solar at low metallicity, favouring a high C production by massive zero-metal supernovae. The [N/Fe] and [N/Mg] ratios scatter widely. Their mean values in each metallicity bin decrease with increasing metallicity, but this trend could be a statistical effect. The larger values of these ratios define a flat upper plateau ([N/Mg] = 0.0, [N/Fe] = +0.1), which could reflect higher values within a wide range of yields of zero-metal SNe II. Alternatively, by analogy with the DLAs, the lower abundances ([N/Mg] = -1.1, [N/Fe] = -0.7) could reflect generally low yields from the first SNe II, the other stars being N enhanced by winds of massive Asymptotic Giant Branch (AGB) stars. Since all the stars show clear [?/Fe] enhancements, they were formed before any significant enrichment of the Galactic gas by SNe Ia, and their composition should reflect the yields of the first SNe II. However, if massive AGB stars or AGB supernovae evolved more rapidly than SNe Ia and contaminated the ISM, our stars would also reflect the yields of these AGB stars. At present it cannot be decided whether primary N is produced primarily in SNe II or in massive AGB stars, or in both. The stellar N abundances and [N/O] ratios are compatible with those found in Damped Lyman-? (DLA) systems. They extend the well-known DLA ``plateau'' at [N/O] ? -0.8 to lower metallicities, albeit with more scatter; no star is found below the putative ``low [N/?] plateau'' at [N/O] ? -1.55 in DLAs. Based on observations obtained with the ESO VLT under ESO programme ID 165.N-0276(A). This work has made use of the SIMBAD database.

Carbocyanine dyes as efficient reversible single-molecule optical switch

Abstract: We demonstrate that commercially available unmodified carbocyanine dyes such as Cy5 (usually excited at 633 nm) can be used as efficient reversible single-molecule optical switch, whose fluorescent state after apparent photobleaching can be restored at room temperature upon irradiation at shorter wavelengths. Ensemble photobleaching and recovery experiments of Cy5 in aqueous solution irradiating first at 633 nm, then at 337, 488, or 532 nm, demonstrate that restoration of absorption and fluorescence strongly depends on efficient oxygen removal and the addition of the triplet quencher β-mercaptoethylamine. Single-molecule fluorescence experiments show that individual immobilized Cy5 molecules can be switched optically in milliseconds by applying alternating excitation at 633 and 488 nm between a fluorescent and nonfluorescent state up to 100 times with a reliability of >90% at room temperature. Because of their intriguing performance, carbocyanine dyes volunteer as a simple alternative for ultrahigh-densit...

Hybrid materials from organophosphorus coupling molecules

Abstract: Organophosphorus acids and their derivatives (salts, esters) are quite complementary of organosilicon coupling molecules for the preparation of hybrid organic–inorganic materials, by sol–gel processing or surface modification. Organosilicon compounds are best suited for the anchoring of organic groups to silicon-containing inorganic matrices or supports, such as silica, silicates, silicon carbide, etc., whereas organophosphorus coupling molecules appear well adapted to matrices or supports based on metals or transition metals: oxides, hydroxides, as well as carbonates and phosphates. The different reactivity of organophosphorus coupling molecules leads to different structures and stabilities of the final hybrid materials and may provide decisive advantages in the sol–gel synthesis of homogeneous hybrids, the preparation of surface monolayers or the selective surface modification of nanopatterned supports.

Discovery of very high energy gamma rays associated with an x-ray binary.

Abstract: X-ray binaries are composed of a normal star in orbit around a neutron star or stellar-mass black hole. Radio and x-ray observations have led to the presumption that some x-ray binaries called microquasars behave as scaled-down active galactic nuclei. Microquasars have resolved radio emission that is thought to arise from a relativistic outflow akin to active galactic nuclei jets, in which particles can be accelerated to large energies. Very high energy γ-rays produced by the interactions of these particles have been observed from several active galactic nuclei. Using the High Energy Stereoscopic System, we find evidence for gamma-ray emission of >100 gigaelectron volts from a candidate microquasar, LS 5039, showing that particles are also accelerated to very high energies in these systems.

Discovery of the Binary Pulsar PSR B1259-63 in Very-High-Energy Gamma Rays around Periastron with H.E.S.S

Abstract: We report the discovery of very-high-energy (VHE) γ-ray emission of the binary system PSR B1259−63/SS 2883 of a radio pulsar orbiting a massive, luminous Be star in a highly eccentric orbit. The observations around the 2004 periastron passage of the pulsar were performed with the four 13 m Cherenkov telescopes of the HESS experiment, recently installed in Namibia and in full operation since December 2003. Between February and June 2004, a γ-ray signal from the binary system was detected with a total significance above 13σ. The flux was found to vary significantly on timescales of days which makes PSR B1259−63 the first variable galactic source of VHE γ-rays observed so far. Strong emission signals were observed in pre- and post-periastron phases with a flux minimum around periastron, followed by a gradual flux decrease in the months after. The measured time-averaged energy spectrum above a mean threshold energy of 380 GeV can be fitted by a simple power law F0(E/ 1T eV) −Γ with a photon index Γ= 2.7 ± 0.2stat ± 0.2sys and flux normalisation F0 = (1.3 ± 0.1stat ± 0.3sys) × 10 −12 TeV −1 cm −2 s −1 .T his detection of VHE γ-rays provides unambiguous evidence for particle acceleration to multi-TeV energies in the binary system. In combination with coeval observations of the X-ray synchrotron emission by the RXTE and INTEGRAL instruments, and assuming the VHE γ-ray emission to be produced by the inverse Compton mechanism, the magnetic field strength can be directly estimated to be of the order of 1 G.

Ontogeny of osmoregulation in postembryonic fish: a review.

Abstract: Salinity and its variations are among the key factors that affect survival, metabolism and distribution during the fish development. The successful establishment of a fish species in a given habitat depends on the ability of each developmental stage to cope with salinity through osmoregulation. It is well established that adult teleosts maintain their blood osmolality close to 300 mosM kg−1 due to ion and water regulation effected at several sites: tegument, gut, branchial chambers, urinary organs. But fewer data are available in developing fish. We propose a review on the ontogeny of osmoregulation based on studies conducted in different species. Most teleost prelarvae are able to osmoregulate at hatch, and their ability increases in later stages. Before the occurrence of gills, the prelarval tegument where a high density of ionocytes (displaying high contents of Na+/K+-ATPase) is located appears temporarily as the main osmoregulatory site. Gills develop gradually during the prelarval stage along with the numerous ionocytes they support. The tegument and gill Na+/K+-ATPase activity varies ontogenetically. During the larval phase, the osmoregulatory function shifts from the skin to the gills, which become the main osmoregulatory site. The drinking rate normalized to body weight tends to decrease throughout development. The kidney and urinary bladder develop progressively during ontogeny and the capacity to produce hypotonic urine at low salinity increases accordingly. The development of the osmoregulatory functions is hormonally controlled. These events are inter-related and are correlated with changes in salinity tolerance, which often increases markedly at the metamorphic transition from larva to juvenile. In summary, the ability of ontogenetical stages of fish to tolerate salinity through osmoregulation relies on integumental ionocytes, then digestive tract development and drinking rate, developing branchial chambers and urinary organs. The physiological changes leading to variations in salinity tolerance are one of the main basis of the ontogenetical migrations or movements between habitats of different salinity regimes.

A new population of very high energy gamma-ray sources in the Milky Way.

Abstract: Very high energy gamma-rays probe the long-standing mystery of the origin of cosmic rays. Produced in the interactions of accelerated particles in astrophysical objects, they can be used to image cosmic particle accelerators. A first sensitive survey of the inner part of the Milky Way with the High Energy Stereoscopic System (HESS) reveals a population of eight previously unknown firmly detected sources of very high energy gamma-rays. At least two have no known radio or x-ray counterpart and may be representative of a new class of "dark" nucleonic cosmic ray sources.

The absorption, metabolism and excretion of flavan-3-ols and procyanidins following the ingestion of a grape seed extract by rats

Abstract: Rats were fed a grape seed extract (GSE) containing (+)-catechin, (-)-epicatechin and dimers, trimers, tetramers and polymeric procyanidins. Liver, kidney, brain and gastrointestinal (GI) tract together with plasma, urine and faeces were collected over a 24 h period and their flavan-3-ol content was analysed by HPLC with tandem mass spectrometry and diode array detection. Small amounts of the GSE flavan-3-ols moved out of the stomach and into the duodenum/jejunum, and to a greater extent the ileum 1 h after ingestion, and into the caecum after 2 h with relatively small amounts being detected in the colon after 3 h. The GI tract contained the parent GSE flavan-3-ols and procyanidins with only trace amounts of metabolites and there were no indications that proanthocyanidins were depolymerised in the GI tract releasing monomeric flavan-3-ols. Plasma contained exclusively catechin glucuronides and methylated glucuronide metabolites which were also detected in the liver and kidneys. These metabolites were also present in urine together with sulphated metabolites and low amounts of the procyanidin dimers B1, B2, B3 and B4 as well as the trimer C2 and an unknown GSE trimer. The amounts of (+)-catechin and (-)-epicatechin metabolites excreted in urine relative to the quantity of the monomers ingested were 27 and 36 %, respectively, after 24 h. This is similar to the levels of urinary excretion reported to occur by other investigators after feeding (-)-epicatechin to rats and provides further, albeit indirect, evidence that the procyanidin oligomers in the GSE were not depolymerised to monomers to any extent after ingestion. No convincing analytical data were obtained for the presence of flavan-3-ol metabolites in the brain.

Heterotachy and long-branch attraction in phylogenetics

Abstract: Probabilistic methods have progressively supplanted the Maximum Parsimony (MP) method for inferring phylogenetic trees. One of the major reasons for this shift was that MP is much more sensitive to the Long Branch Attraction (LBA) artefact than is Maximum Likelihood (ML). However, recent work by Kolaczkowski and Thornton suggested, on the basis of simulations, that MP is less sensitive than ML to tree reconstruction artefacts generated by heterotachy, a phenomenon that corresponds to shifts in site-specific evolutionary rates over time. These results led these authors to recommend that the results of ML and MP analyses should be both reported and interpreted with the same caution. This specific conclusion revived the debate on the choice of the most accurate phylogenetic method for analysing real data in which various types of heterogeneities occur. However, variation of evolutionary rates across species was not explicitly incorporated in the original study of Kolaczkowski and Thornton, and in most of the subsequent heterotachous simulations published to date, where all terminal branch lengths were kept equal, an assumption that is biologically unrealistic. In this report, we performed more realistic simulations to evaluate the relative performance of MP and ML methods when two kinds of heterogeneities are considered: (i) within-site rate variation (heterotachy), and (ii) rate variation across lineages. Using a similar protocol as Kolaczkowski and Thornton to generate heterotachous datasets, we found that heterotachy, which constitutes a serious violation of existing models, decreases the accuracy of ML whatever the level of rate variation across lineages. In contrast, the accuracy of MP can either increase or decrease when the level of heterotachy increases, depending on the relative branch lengths. This result demonstrates that MP is not insensitive to heterotachy, contrary to the report of Kolaczkowski and Thornton. Finally, in the case of LBA (i.e. when two non-sister lineages evolved faster than the others), ML outperforms MP over a wide range of conditions, except for unrealistic levels of heterotachy. For realistic combinations of both heterotachy and variation of evolutionary rates across lineages, ML is always more accurate than MP. Therefore, ML should be preferred over MP for analysing real data, all the more so since parametric methods also allow one to handle other types of biological heterogeneities much better, such as among sites rate variation. The confounding effects of heterotachy on tree reconstruction methods do exist, but can be eschewed by the development of mixture models in a probabilistic framework, as proposed by Kolaczkowski and Thornton themselves.

Pressure sensitivity of olivine slip systems and seismic anisotropy of Earth's upper mantle.

Abstract: The mineral olivine dominates the composition of the Earth's upper mantle and hence controls its mechanical behaviour and seismic anisotropy Experiments at high temperature and moderate pressure, and extensive data on naturally deformed mantle rocks, have led to the conclusion that olivine at upper-mantle conditions deforms essentially by dislocation creep with dominant [100] slip The resulting crystal preferred orientation has been used extensively to explain the strong seismic anisotropy observed down to 250 km depth The rapid decrease of anisotropy below this depth has been interpreted as marking the transition from dislocation to diffusion creep in the upper mantle But new high-pressure experiments suggest that dislocation creep also dominates in the lower part of the upper mantle, but with a different slip direction Here we show that this high-pressure dislocation creep produces crystal preferred orientations resulting in extremely low seismic anisotropy, consistent with seismological observations below 250 km depth These results raise new questions about the mechanical state of the lower part of the upper mantle and its coupling with layers both above and below

Implications of WMAP Observations on Li Abundance and Stellar Evolution Models

Abstract: The Wilkinson Microwave Anisotropy Probe (WMAP) determination of the baryon-to-photon ratio implies, through big bang nucleosynthesis, a cosmological Li abundance larger, by a factor of 2-3, than the Li abundance plateau observed in the oldest Population II stars. It is, however, inescapable that there be a reduction by a factor of at least 1.6-2.0 of the surface Li abundance during the evolution of Population II field stars with [Fe/H] ≤ -1.5. That the observed Li should be lower than cosmologically produced Li is expected from stellar evolution models. Since at turnoff most of the Li abundance reduction is caused by gravitational settling, the presence of 6Li in some turnoff stars is also understood. Given that the WMAP implications for Li cosmological abundance and the Li Spite plateau can be naturally explained by gravitational settling in the presence of weak turbulence, there appears little need for exotic physics as suggested by some authors. Instead, there is a need for a better understanding of turbulent transport in the radiative zones of stars. This requires simulations from first principles. Rather strict upper limits to turbulent transport are determined for the Sun and Population II stars.

Submarine fault scarps in the Sea of Marmara pull-apart (North Anatolian Fault): Implications for seismic hazard in Istanbul

Abstract: Earthquake scarps associated with recent historical events have been found on the floor of the Sea of Marmara, along the North Anatolian Fault (NAF). The MARMARASCARPS cruise using an unmanned submersible (ROV) provides direct observations to study the fine-scale morphology and geology of those scarps, their distribution, and geometry. The observations are consistent with the diversity of fault mechanisms and the fault segmentation within the north Marmara extensional step-over, between the strike-slip Ganos and Izmit faults. Smaller strike-slip segments and pull-apart basins alternate within the main step-over, commonly combining strike-slip and extension. Rapid sedimentation rates of 1?3 mm/yr appear to compete with normal faulting components of up to 6 mm/yr at the pull-apart margins. In spite of the fast sedimentation rates the submarine scarps are preserved and accumulate relief. Sets of youthful earthquake scarps extend offshore from the Ganos and Izmit faults on land into the Sea of Marmara. Our observations suggest that they correspond to the submarine ruptures of the 1999 Izmit (Mw 7.4) and the 1912 Ganos (Ms 7.4) earthquakes. While the 1999 rupture ends at the immediate eastern entrance of the extensional Cinarcik Basin, the 1912 rupture appears to have crossed the Ganos restraining bend into the Sea of Marmara floor for 60 km with a right-lateral slip of 5 m, ending in the Central Basin step-over. From the Gulf of Saros to Marmara the total 1912 rupture length is probably about 140 km, not 50 km as previously thought. The direct observations of submarine scarps in Marmara are critical to defining barriers that have arrested past earthquakes as well as defining a possible segmentation of the contemporary state of loading. Incorporating the submarine scarp evidence modifies substantially our understanding of the current state of loading along the NAF next to Istanbul. Coulomb stress modeling shows a zone of maximum loading with at least 4?5 m of slip deficit encompassing the strike-slip segment 70 km long between the Cinarcik and Central Basins. That segment alone would be capable of generating a large-magnitude earthquake (Mw 7.2). Other segments in Marmara appear less loaded.

Beyond the Mediterranean peninsulas: evidence of central European glacial refugia for a temperate forest mammal species, the bank vole (Clethrionomys glareolus)

Abstract: This study details the phylogeographic pattern of the bank vole, Clethrionomys glareolus,a European rodent species strongly associated with forest habitat. We used sequences of 1011 base pairs of the mitochondrial DNA cytochrome b gene from 207 bank voles collected in 62 localities spread throughout its distribution area. Our results reveal the presence of three Mediterranean (Spanish, Italian and Balkan) and three continental (western, eastern and ‘Ural’) phylogroups. The endemic Mediterranean phylogroups did not contribute to the postglacial recolonization of much of the Palaearctic range of species. Instead, the major part of this region was apparently recolonized by bank voles that survived in glacial refugia in central Europe. Moreover, our phylogeographic analyses also reveal differentiated populations of bank voles in the Ural mountains and elsewhere, which carry the mitochondrial DNA of another related vole species, the ruddy vole (Clethrionomys rutilus). In conclusion, this study demonstrates a complex phylogeographic history for a forest species in Europe which is sufficiently adaptable that, facing climate change, survives in relict southern and northern habitats. The high level of genetic diversity characterizing vole populations from parts of central Europe also highlights the importance of such regions asa source of intraspecific genetic biodiversity.

Relaxed Molecular Clock Provides Evidence for Long-Distance Dispersal of Nothofagus (Southern Beech)

Abstract: Nothofagus (southern beech), with an 80-million-year-old fossil record, has become iconic as a plant genus whose ancient Gondwanan relationships reach back into the Cretaceous era. Closely associated with Wegener's theory of “Kontinentaldrift”, Nothofagus has been regarded as the “key genus in plant biogeography”. This paradigm has the New Zealand species as passengers on a Moa's Ark that rafted away from other landmasses following the breakup of Gondwana. An alternative explanation for the current transoceanic distribution of species seems almost inconceivable given that Nothofagus seeds are generally thought to be poorly suited for dispersal across large distances or oceans. Here we test the Moa's Ark hypothesis using relaxed molecular clock methods in the analysis of a 7.2-kb fragment of the chloroplast genome. Our analyses provide the first unequivocal molecular clock evidence that, whilst some Nothofagus transoceanic distributions are consistent with vicariance, trans-Tasman Sea distributions can only be explained by long-distance dispersal. Thus, our analyses support the interpretation of an absence of Lophozonia and Fuscospora pollen types in the New Zealand Cretaceous fossil record as evidence for Tertiary dispersals of Nothofagus to New Zealand. Our findings contradict those from recent cladistic analyses of biogeographic data that have concluded transoceanic Nothofagus distributions can only be explained by vicariance events and subsequent extinction. They indicate that the biogeographic history of Nothofagus is more complex than envisaged under opposing polarised views expressed in the ongoing controversy over the relevance of dispersal and vicariance for explaining plant biodiversity. They provide motivation and justification for developing more complex hypotheses that seek to explain the origins of Southern Hemisphere biota.

Simian Immunodeficiency Virus Infection in Free-Ranging Sooty Mangabeys (Cercocebus atys atys) from the Taï Forest, Côte d'Ivoire: Implications for the Origin of Epidemic Human Immunodeficiency Virus Type 2

Abstract: Simian immunodeficiency virus of sooty mangabeys (SIVsmm) is recognized as the progenitor of human immunodeficiency virus type 2 (HIV-2) and has been transmitted to humans on multiple occasions, yet the epidemiology and genetic diversity of SIVsmm infection in wild-living populations remain largely unknown. Here, we report the first molecular epidemiological survey of SIVsmm in a community of 120 free-ranging sooty mangabeys in the TaiForest, Cote d'Ivoire. Fecal samples (n 39) were collected from 35 habituated animals (27 females and 8 males) and tested for SIVsmm virion RNA (vRNA). Viral gag (800 bp) and/or env (490 bp) sequences were amplified from 11 different individuals (eight females and three males). Based on the sensitivity of fecal vRNA detection and the numbers of samples analyzed, the prevalence of SIVsmm infection was estimated to be 59% (95% confidence interval, 0.35 to 0.88). Behavioral data collected from this community indicated that SIVsmm infection occurred preferentially in high-ranking females. Phylogenetic analysis of gag and env sequences revealed an extraordinary degree of genetic diversity, including evidence for frequent recombination events in both the recent and distant past. Some sooty mangabeys harbored near-identical viruses (<2% interstrain distance), indicating epidemiologically linked infections. These transmissions were identified by microsatellite analyses to involve both related (mother/daughter) and unrelated individuals, thus providing evidence for vertical and horizontal transmission in the wild. Finally, evolutionary tree analyses revealed significant clustering of the TaiSIVsmm strains with five of the eight recognized groups of HIV-2, including the epidemic groups A and B, thus pointing to a likely geographic origin of these human infections in the eastern part of the sooty mangabey range.