Showing papers by "University of Naples Federico II published in 1972"
TL;DR: Two isomeric linear difurano sesterterpenes, ircininin-1 and -2, have been identified in the sponge Ircinia oros as mentioned in this paper.
73 citations
TL;DR: The mechanism underlying the formation of RB and the possible significance that they may have for protein synthesis during oogenesis are discussed.
59 citations
58 citations
TL;DR: In 48 patients suspected for hepatic neoplasm, a combined procedure was carried out, including a blind transthoracic biopsy of the liver, peritoneoscopy, and a second biopsy done under peritoneoscopic guidance, which improved diagnostic accuracy to 75% of cases.
52 citations
TL;DR: Indomethacin was about 4 times more effective in inhibiting prostaglandin E 2 than in counteracting histamine, 5-hydroxytryptamine and bradykinin.
42 citations
01 Jan 1972
TL;DR: In this article, a probabilistic fomulation of the fundamental theorems of "limit analysis" is developed, which allows, with a very limited amount of computing work, the determination of a domain containing the probability distribution curve of the collapse load factor of any structure that satisfies the usual conditions for validity of the limit analysis, but has randomly distributed limit strengths.
Abstract: On the basis of a probabilistic fomulation of the fundamental theorems of “limit analysis,” a procedure is developed which allows, with a very limited amount of computing work, the determination of a domain containing the probability distribution curve of the collapse load factor of any structure that satisfies the usual conditions for validity of the limit analysis, but has randomly distributed limit strengths. Further improvements of the bounds thus obtained can be achieved by the equivalent of either the equilibrium or the kinematic methods of limit analysis.
41 citations
TL;DR: This work demonstrates that the deficiency of α-galactosidase is caused by an X-linked mutation, but does not necessarily prove that the structural locus for this enzyme is itself located on the X-chromosome.
Abstract: A deficiency in α-galactosidase (α-Gal) activity–as measured aspecifically with the use of artificial substrates–is a regular feature in leucocytes and fibroblasts of patients affected by angiokeratoma corporis diffusum or Fabry's disease, a well-known X-linked trait in man1–4. Fibroblast clones derived from mothers of affected males exhibit either normal or deficient activity of α-galactosidase. This demonstrates that the deficiency of α-galactosidase is caused by an X-linked mutation, but does not necessarily prove that the structural locus for this enzyme is itself located on the X-chromosome.
40 citations
TL;DR: In this paper, five difuranoterpenes having the same carbon skeleton as furospongin-1 have been identified in the sponges Spongia officinalis and Hippospongia communis.
38 citations
TL;DR: Hog and bovine 27 S thyroid iodoprotein, incubated for prolonged periods at 4 °C and neutral pH, formed 19 S and 2.5 S species which were isolated by gel filtration, suggesting the possible existence of intrachain, repeating, homologous sequences in the 27 S iodop protein.
34 citations
TL;DR: It is concluded from these isotopic studies that a number of healthy and uraemic men can synthesise phenylalanine and valine from their ketoacid analogues.
32 citations
TL;DR: A mechanism of synthesis at the polymer level of the two DNA ‘minor’ bases by the action of specific DNA cytosine methylases and DNA-5 methylcytosine aminohydrolases is suggested as the possible chemical basis of the synchron model of cell differentiation.
TL;DR: Brush border membranes isolated from rat intestine hydrolyzed β-naphthylamides of many amino acids, l -leucinamide and l -phenylalanyl- l -alanine with high specific activities with highspecific activities as regards pH optimum, ion dependence and puromycin inhibition.
TL;DR: Levels of ampicillin in serum, bile, gallbladder wall, and gallstones were found to be higher after iv administration than after oral administration, and S. typhi was absent from the biles, the wall of the gallbladders, and the calculi of one patient who later underwent cholecystectomy.
Abstract: Nineteen carriers of Salmonella typhi were studied; five had gallstones, and the others were documented biliary-tract carriers of S. typhi. One patient had already undergone cholecystectomy. A dose of ampicillin (1 g) was administered iv every 8 hr for 15 days. In all cases, cultures of stool became negative during treatment and remained so throughout the 16to 24-month period of follow-up. S. typhi was absent from the bile, the wall of the gallbladder, and the calculi of one patient who later underwent cholecystectomy. In a second part of this study, a single dose of ampicillin (1 g) was given either orally or iv before cholecystectomy to two groups of four patients each. Levels of ampicillin in serum, bile, gallbladder wall, and gallstones were found to be higher after iv administration than after oral administration.
TL;DR: Inhibition of the enzyme activity by several carbonyl reagents suggests an involvement of either pyridoxal phosphate or pyruvate in the catalytic process and the inhibitory effect of thiol reagents indicates the presence of ;essential' thiol groups.
Abstract: 1. The presence of S -adenosylmethionine decarboxylase in human prostate gland is reported. A satisfactory radiochemical enzymic assay was developed and the enzyme was partially characterized. 2. Putrescine stimulates the reaction rate by up to 6-fold at pH7.5: the apparent activation constant was estimated to be 0.13mm. The stimulation is pH-dependent and a maximal effect is observed at acid pH values. 3. Putrescine activation is rather specific: other polyamines, such as spermidine and spermine, did not show any appreciable effect. 4. The apparent K m for the substrate is 4×10 −5 m. The calculated S -adenosylmethionine content of human prostate (0.18μmol/g wet wt. of tissue) demonstrates that the cellular amounts of sulphonium compound are saturating with respect to the enzyme. 5. The enzyme is moderately stable at 0°C and is rapidly inactivated at 40°C. The optimum pH is about 7.5, with one-half of the maximal activity occurring at pH6.6. 6. Several carboxy - 14 C-labelled analogues and derivatives of S -adenosylmethionine were tested as substrates. The enzyme appears to be highly specific: the replacement of the 6′-amino group of the sulphonium compound alone results in a complete loss of activity. 7. Inhibition of the enzyme activity by several carbonyl reagents suggests an involvement of either pyridoxal phosphate or pyruvate in the catalytic process. 8. The inhibitory effect of thiol reagents indicates the presence of ‘essential’ thiol groups.
TL;DR: The present study was undertaken to investigate the cardiac effects of 5 antidepressive tricyclic derivatives namely imipramine, desipramines, amitriptyline, trimeprimine and noxiptiline and their anti-arrhythmic and anticoronarospastic activity in vivo and in vitro.
TL;DR: The synthesis of marrubiin has been achieved starting from the keto lactone (II) which was prepared stereoselectively from the known keto ester (XI).
TL;DR: It is suggested that progression of uremia might not represent the main cause of decrease in the Ep response to hypoxia following nephrectomy, and a decrease in REF activity might be responsible for the decline.
TL;DR: The ectopic pars distalis graft showed a general cytological disorganization and regression of the various cell types and its gonadotropic function appeared to be extremely poor.
Abstract: Removal of the pars distalis, homoiotopic and ectopic autografting of the pars distalis, and transection of the hypothalamo-hypophyseal link was done and their effect upon the cytology of the pars distalis and its gonadotropic functions was studied in the male green frog, Rana esculenta. Removal of pars distalis led to the regression of gonads and secondary sex organs. The gonadotropic activity of the pars distalis regrafted under the median eminence was comparable to that of the sham-operated control except that in the gland oriented at an angle of 180° the thumb pads were involuted. The normal functional activity of the regrafted gland can be explained by the contact re-established between the grafted gland and the portal vessels at the level of the median eminence as ascertained by injections of India ink. The ectopic pars distalis graft showed a general cytological disorganization and regression of the various cell types and its gonadotropic function appeared to be extremely poor. Transection of the hypothalamo-hypophyseal connections diminished the gonadotropic effectiveness of the pars distalis.
TL;DR: The helix sense of the L‐aspartate residues determined from the αCH and NH chemical shifts agrees with that obtained from ORD, and the stability of the helical copolymers to TFA addition shows a minimum at the composition of helix‐sense inversion for the L-glutamate:L‐as Partate copolymer but no minimum for the lefthanded D‐glUTamate: L‐ aspartate series.
Abstract: High-resolution NMR is used to study two series of benzyl-L- or D-glutamate: benzyl-L-aspartate random copolymers. The helix sense of the L-aspartate residues determined from the αCH and NH chemical shifts agrees with that obtained from ORD. The stability of the helical copolymers to TFA addition shows a minimum at the composition of helix-sense inversion for the L-glutamate:L-aspartate copolymers but no minimum for the lefthanded D-glutamate: L-aspartate series. The helix-coil transition of the glutamate and aspartate residues in each polymer is compared. For the D-glutamate: L-aspartate series no differences are found, indicating random copolymerization. In the L-glutamate:L-aspartate series significant differences between the transition midpoints are interpreted as resulting from irregular distribution of component residues along the chain.
01 Jan 1972
TL;DR: It is concluded that Methallibure is a non-steroidal antigonadotropic compound and the important question about its mode of action has been brought into discussion.
Abstract: The influence of ICI compound 33,828 (1-α-methylallylthiocarbamoyl-2-methylthiocarbamoylhydrazine = Methallibure) on the pars distalis of pituitary, testes and thumb pads was investigated in the intact adult male green frog, Rana esculenta. Methallibure affected the gonadotropic basophils (particularly the B2 cells) of the pars distalis, which showed varying degrees of degranulation and underwent a notable decrease in their percentage. Within the testis this compound caused the arrest of spermatogenesis. The most uniform effect of Methallibure was observed in the thumb pads, which invariably showed regression of the epidermis and glandular epithelium. No histological changes occurred in the thyroid and adrenal glands and the B1 and A1 cells of the pars distalis remained unchanged cytologically. It is concluded, in concordance with the available data, that Methallibure is a non-steroidal antigonadotropic compound. The important question about its mode of action has been brought into discussion.
TL;DR: Sono state studiate le variazioni di alcuni enzimi microsomiali del fegato di topi trattati con fenobarbital in varie fasi della epatite da MHV-3 in fasi di avanzata citonecrosi epatica.
Abstract: Sono state studiate le variazioni di alcuni enzimi microsomiali del fegato di topi trattati con fenobarbital in varie fasi della epatite da MHV-3. E stato osservato che anche in fasi di avanzata citonecrosi epatica il trattamento con fenobarbital determina netti incrementi del citocromo P-450, della NADPH ossidasi e della anilina idrossilasi microsomiali. Nelle stesse condizioni invece la glucosio-6-fosfatasi mostra un decremento sia nei topi controllo che negli infetti.
TL;DR: The presence of steroid transforming enzymes in the gonads of Ciona intestinalis leads us to presume that sex hoimone biosynthesis of protochordates has long preceded the pattern present in veitebiates.
Abstract: Andiogui seciction by llie lestis has been demonstrated in several repiesentatives of all classes of veitebiates, and sex hormone biosynthesis does not show lcmaikdble difTeienccs among them however, it is not known which is the trueindiogcn uoiking m vivo in nonmammahan vertebiates
The presence of steroid transforming enzymes in the gonads of Ciona intestinalis leads us to presume that sex hoimone biosynthesis of protochordates has long preceded the pattern present in veitebiates
The site of steroid hormone biosynthesis has been shown to be the interstitial tissue although in the embryonic testis of Sauropsida 3β hydioxysteroid dehydrogenase is present only in the seminifeious tubules. These findings are discussed in the light of embiyological and experimental research.
TL;DR: Methallibure blocks the stimulation of these organs by homoplastic pars distalis, which indicates that this compound inhibits the action of gonadotropic hormones upon their target tissues.
Abstract: Effects of Methallibure on the gonadotropic stimulation of the regressed gonads and secondary sex characters of the pars distalis-ectomized adult green frog, Rana esculenta, were studied Regression of the gametogenetic activity and reduction of oviducts and thumb pads are the eventual consequences of the removal of pars distalis Homoplastic pars distalis homogenates restimulate the gametogenesis and regressed secondary sex characters due to their gonadotropic hormone contents Methallibure in this case blocks the stimulation of these organs by homoplastic pars distalis, which indicates that this compound inhibits the action of gonadotropic hormones upon their target tissues
TL;DR: In this paper, a triazoline III/diazoethane IV mutual conversion was investigated and the behavior of the latter and its isomerization product, 1-phenyl-2, 2,2-dimethoxy-2 (N-ethoxyearbonylamino)diazosethane (IV) was investigated.
TL;DR: It appears that the elaboration phenomena occurring in both anterolateral and posterior areas of the cerebral organ have no relation with neurosecretion of the worm brain, and that the products elaborated by the gland and nerve cells ofThe cerebral organ are mostly carbohydrate in nature.
TL;DR: The data suggest that both amino groups of the molecule are involved in the activation of lysine-2,3-aminomutase by S-adenosylmethionine in Clostridium SB4, and that the steric configuration of the sulfonium pole is not related to the activation mechanism.