Showing papers by "University of Naples Federico II published in 1986"
TL;DR: In this paper, the complications that arose after percutaneous liver biopsies performed from 1973 to 1983 were analyzed in relation to the underlying liver disease and to the type of needle used.
1,163 citations
TL;DR: Both the urokinase‐type and tissue‐type plasminogen activator can convert their 5̃4 kDa type‐1 inhibitor (PAI‐1) to an inactive form with a lower apparent molecular mass.
208 citations
TL;DR: It is concluded that only one gene is responsible for the synthesis of the majority of apoferritin H mRNA in various tissues examined, and that probably all the other DNA segments hybridizing with apferritin cDNA are pseudogenes.
Abstract: Ferritin is composed of two subunits, H and L. cDNA's coding for these proteins from human liver (1,2,3), lymphocytes (4) and from the monocyte-like cell line U937 (5) have been cloned and sequenced. Southern blot analysis on total human DNA reveals that there are many DNA segments hybridizing to the apoferritin H and L cDNA probes (1,2,4,6). In view of the tissue heterogeneity of ferritin molecules (7,8), it appeared possible that apoferritin molecules could be coded by a family of genes differentially expressed in various tissues (1,2). In this paper we describe the cloning and sequencing of the gene coding for human apoferritin H. This gene has three introns; the exon sequence is identical to that of cDNA's isolated from human liver, lymphocytes, HeLa cells and endothelial cells. In addition we show that at least 15 intronless pseudogenes exist, with features suggesting that they were originated by reverse transcription and insertion. On the basis of these results we conclude that only one gene is responsible for the synthesis of the majority of apoferritin H mRNA in various tissues examined, and that probably all the other DNA segments hybridizing with apoferritin cDNA are pseudogenes.
112 citations
TL;DR: In this article, an experimental study of metal ion separations by supported liquid membranes is described, which use the extraction reagents D2EHPA, LIX 64N, DNNSA and Aliquat 336 as carriers.
106 citations
TL;DR: The occurrence of esophageal motility disorders only in patients with severe esophagitis and its disappearance after therapy may account for the favorable course of reflux disease in infancy.
99 citations
TL;DR: The data support the idea that the laxative effects of these intestinal secretagogues are due to increased intestinal production of PG‐LM, histamine and 5‐HT.
Abstract: The effects of some laxatives were examined on the formation of histamine, 5-hydroxytryptamine (5-HT) and prostaglandin-like material (PG-LM) by rat intestine in-vitro. Castor oil, senna, sulphosuccinate and bisacodyl, but not mannitol or lactulose, in doses that cause laxation, increased the formation of histamine, 5-HT and PG-LM. Indomethacin or hydrocortisone reduced the increase of PG-LM formation. The data support the idea that the laxative effects of these intestinal secretagogues are due to increased intestinal production of PG-LM, histamine and 5-HT.
97 citations
TL;DR: It is suggested that insulin induces a shift of magnesium from the plasma to the erythrocytes both in vivo and in vitro, which may help to interprete the abnormalities in magnesium circulating levels frequently reported in diabetic patients.
Abstract: Plasma and erythrocyte magnesium levels were measured by atomic absorption spectrophotometry in 10 healthy volunteers during an oral glucose tolerance test and during an euglycaemic hyperinsulinaemic glucose clamp. At min 180 and 210 of the oral glucose tolerance test, a significant decline in plasma magnesium levels (p less than 0.01 and p less than 0.05 respectively) and a significant increase in erythrocyte magnesium levels (p less than 0.01 and p less than 0.05 respectively) were observed. Similar changes were seen during the second hour of the glucose clamp, during which euglycaemia (4.1 +/- 0.4 mmol/l) was maintained despite hyperinsulinaemia (110-130 mU/l). During in vitro incubations, glucose (5 mmol/l) did not modify erythrocyte magnesium levels. In contrast, erythrocyte magnesium levels were significantly increased (p less than 0.01) by insulin (100 mU/l), an effect entirely abolished by ouabain (5 X 10(-4) mol/l). These results suggest that insulin induces a shift of magnesium from the plasma to the erythrocytes both in vivo and in vitro. These data may help to interprete the abnormalities in magnesium circulating levels frequently reported in diabetic patients.
94 citations
82 citations
TL;DR: A new biochemical method for the quantitation of myocardial scarring, based on the determination of left ventricular hydroxyproline, and collagen content at the end of the healing process is described, verified in rats with coronary artery occlusion and in a treated group receiving nitroglycerin.
78 citations
TL;DR: In this article, the authors present some rheological properties of selected pasta filata cheeses and propose an explanation of the mechanism responsible for stress relaxation, which is suggested that stresses relax through breakage and reformation of weak bonds in the protein network.
72 citations
TL;DR: An examination of the ovarian hormone secretion pattern in P. dacnicolor during the year revealed that plasma levels of testosterone and estradiol correlated with ovarian growth and attained highest levels in amplectant and ovulating females.
TL;DR: The authors report 40 personal cases of infratentorial hemangioblastomas and review 36 series from the literature for a total number of 1023 patients, with particular consideration of localizations outside the nervous system, multicentric tumors, recurrences, and long-term results.
TL;DR: Basophils from patients with AD released significantly more histamine both "spontaneously" and in response to D2O than did controls and there was a significant correlation between the sensitivity of the cells to release with f-met-peptide and the response to A23187 both in control and in AD patients.
TL;DR: The data, which confirm previous reports of serum lipid changes during antihypertensive therapy, suggest that alpha1 blockers may interfere with lipoprotein lipase, possibly by reducing its catecholamine-mediated inactivation.
01 Jan 1986
TL;DR: Based on the low cost, reliability and simple use, intrarectal ultrasound is proposed as first examination for local recurrence detection in the follow-up of patients with low anterior resection for rectal cancer.
Abstract: The ability of intrarectal ultrasound to recognize the local extent of disease was investigated in 23 patients with histologically proven adenocarcinoma of the lower two-thirds of the rectum before operation. Two probes, 12 cm long, working at a frequency of 3·5 and 7·5 MHz, were used. The results were compared with those of pre-operative computed tomography (CT) and with the pathological report of the resected specimens. Sonography correctly staged 20 of 23 tumours with two false negatives and one false positive, while CT correctly staged 19 of 23 tumours with two false negatives and two false positives. The results of ultrasound were found to be as accurate as those of CT; the low cost and simple use of ultrasound makes it preferable in the pre-operative assessment of the depth of invasion of rectal cancer. In addition, intrarectal ultrasound was routinely performed in 42 patients, operated on for rectal cancer by means of sphincter-saving procedures, at variable intervals in the first 2 years postoperatively. Eight local recurrences were recognized and confirmed by CT. Based on the low cost, reliability and simple use, intrarectal ultrasound is proposed as first examination for local recurrence detection in the follow-up of patients with low anterior resection for rectal cancer.
TL;DR: In this paper, the electrochemical behavior of graphite-epoxy composite materials (GECM) in neutral 3.5% aqueous solutions of NaCl has been investigated.
Abstract: The electrochemical behaviour of graphite-epoxy composite materials (GECM) in neutral 3.5% aqueous solutions of NaCl has been investigated. Two types of composite and pure graphite have been studied. The composite material shows a high value of electrode potential ranging between −40 and +70 mV versus SCE, and behaves as cathode when coupled to alloys.
TL;DR: The acute stress associated with major disasters can influence risk factors for coronary heart disease and permanent elevation of these risk factors due to the disruption of the social environment of the individuals affected by major disasters might be responsible for the apparent long-term adverse effects on cardiovascular mortality.
Abstract: In this analysis of the data from a longitudinal study on coronary heart disease risk factors, it was found that participants screened a few weeks after a major disaster (earthquake) had a higher heart rate, serum cholesterol levels, and serum triglyceride levels than matched participants that were screened shortly before the catastrophic event. The two groups of participants did not differ with regard to their characteristics at the baseline examination carried out 5 years previously. The lack of difference in blood pressure between exposed and nonexposed participants could be explained by the lag-time between the earthquake and the blood pressure measurements. We conclude that the acute stress associated with major disasters can influence risk factors for coronary heart disease. Permanent elevation of these risk factors due to the disruption of the social environment of the individuals affected by major disasters might be responsible for the apparent long-term adverse effects on cardiovascular mortality discussed previously in the literature.
TL;DR: The plasma levels of vitamin E were significantly higher in treated than in control subjects and animals, and vitamin E administration did not influence ascorbic-acid levels in plasma, aqueous humor, lens and vitreous body (rabbits).
TL;DR: It is shown for both two- and three-trophic level systems mentioned above that the evolutionarily stable state with respect to switching parameters is dynamically stable.
TL;DR: In this paper, the existence of global weak solutions for 2-dimensional unsteady viscous compressible flows of an ideal isothermal gas was proved by a suitable coupling of the method of Hopf [17] with standard techniques in Orlicz spaces.
TL;DR: On the while alternate‐day prednisone therapy does not affect statural growth and bone maturation of children with lipoid nephrosis, it is found that bone age fell within the normal range for chronological age in all the children studied.
Abstract: Statural growth has been evaluated in 20 prepubertal nephrotic children who received alternate-day prednisone therapy for a year at least. Bone age was assessed in 16 of these children after 1-4 years of therapy. During the follow-up 12 children showed variations in height standard deviation score (SDs) below 0.5, 7 gained more than 0.5 SDs and 1 lost 0.5 SDs. Bone age fell within the normal range for chronological age in all the children studied. On the while alternate-day prednisone therapy does not affect statural growth and bone maturation of children with lipoid nephrosis.
TL;DR: Using a translation assay, it is shown that the axoplasm of the squid giant axon contains significant amounts of mRNA coding for a heterogeneous group of prot sets of proteins specified by glial and neuronal perikaryal mRNA.
TL;DR: G elements lack terminal repeats, generate target site duplications at the point of insertion and exhibit at one end a stretch of A residues of variable length, suggesting that distinct DNA sequences might have been inserted into rDNA through a partly common pathway.
Abstract: The properties and the genomic organization of G elements, a moderately repeated DNA family of D. melanogaster, are reported. G elements lack terminal repeats, generate target site duplications at the point of insertion and exhibit at one end a stretch of A residues of variable length. In a large number of recombinant clones analyzed G elements occur in tandem arrays, interspersed with specific ribosomal DNA (rDNA) segments. This arrangement results from the insertion of members of the G family within the nontranscribed spacer (NTS) of rDNA units. Similarity of the site of integration of G elements to that of ribosomal DNA insertions suggests that distinct DNA sequences might have been inserted into rDNA through a partly common pathway.
TL;DR: Marone et al. as discussed by the authors compared the effect of zinc chloride on the release of chemical mediators from human basophils and mast cells isolated from human lung and found that zinc chloride was a competitive antagonist of the action of Ca2+ in histamine secretion induced by anti-IgE with a dissociation constant (Kd) of about 10(-5) M in both the basophil and mast cell systems.
Abstract: We have previously shown that physiological concentrations of zinc (congruent to 7 X 10(-6) M) inhibit the release of histamine from human basophil leukocytes (Marone et al., J. Pharmacol. Exp. Ther. 217: 292, 1981). In these experiments we compared the effect of zinc chloride on the release of chemical mediators from human basophils and mast cells isolated from human lung. Preincubation (5 min, 37 degrees C) of human basophils and lung mast cells with zinc chloride (10(-6)-3 X 10(-5) M) caused dose-related inhibition of histamine and peptide leukotriene C4 (LTC4) release induced by anti-IgE. Increase Ca2+ concentrations (0.3 to 6 mM) in the extracellular medium completely reversed the inhibitory effect of zinc on anti-IgE-mediated histamine secretion. Zinc chloride was a competitive antagonist of the action of Ca2+ in histamine secretion induced by anti-IgE with a dissociation constant (Kd) of about 10(-5) M in both the basophil and mast cell systems. Thus physiological concentrations of zinc inhibit the release of histamine from human basophils and lung mast cells, presumably by blocking Ca2+ uptake induced by anti-IgE activation.
TL;DR: Gonadotropin-releasing hormone molecular forms in the brains of three reptiles, Alligator mississippiensis (alligator), Calcides ocellatus tiligugu (skink) and Podarcis s.
TL;DR: In this paper, the longitudinal and radial electric field components generated by the propagation of two electromagnetic waves resonantly beating in a plasma were obtained, assuming Gaussian profiles for the pump amplitudes.
Abstract: In a simple, linear fluid model we obtain the longitudinal and radial electric field components generated by the propagation of two electromagnetic waves resonantly beating in a plasma, assuming Gaussian profiles for the pump amplitudes.
TL;DR: In this article, a psychometric study was carried out on Australian, Danish, Hungarian, Italian and Dutch non-patient samples examining cross-national invariance characteristics of dimensions of perceived parental rearing behaviour and attitudes as measured by the EMBU.
TL;DR: It is suggested that assays of antiestrogen and antiprogestin drugs in cultured cells can indicate whether this estrogen and progesterone receptor activity may be of therapeutic use.
TL;DR: The two strategies are interdependent and additive and should be introduced in parallel to lessen the burden of CHD in the population as a whole and in the very-high-risk minority within the population.