Showing papers by "University of Naples Federico II published in 1997"
TL;DR: It is demonstrated that LDL oxidation and formation of fatty streaks occurs already during fetal development, and that both phenomena are greatly enhanced by maternal hypercholesterolemia.
Abstract: To determine whether oxidized LDL enhances atherogenesis by promoting monocyte recruitment into the vascular intima, we investigated whether LDL accumulation and oxidation precede intimal accumulation of monocytes in human fetal aortas (from spontaneous abortions and premature newborns who died within 12 h; fetal age 6.2+/-1.3 mo). For this purpose, a systematic assessment of fatty streak formation was carried out in fetal aortas from normocholesterolemic mothers (n = 22), hypercholesterolemic mothers (n = 33), and mothers who were hypercholesterolemic only during pregnancy (n = 27). Fetal plasma cholesterol levels showed a strong inverse correlation with fetal age (R = -0.88, P < 0.0001). In fetuses younger than 6 mo, fetal plasma cholesterol levels correlated with maternal ones (R = 0.86, P = 0.001), whereas in older fetuses no such correlation existed. Fetal aortas from hypercholesterolemic mothers and mothers with temporary hypercholesterolemia contained significantly more and larger lesions (758,651+/-87,449 and 451,255+/-37,448 micron2 per section, respectively; mean+/-SD) than aortas from normocholesterolemic mothers (61,862+/-9,555 micron2; P < 0.00005). Serial sections of the arch, thoracic, and abdominal aortas were immunostained for recognized markers of atherosclerosis: macrophages, apo B, and two different oxidation-specific epitopes (malondialdehyde- and 4-hydroxynonenal-lysine). Of the atherogenic sites that showed positive immunostaining for at least one of these markers, 58.6% were established lesions containing both macrophage/foam cells and oxidized LDL (OxLDL). 17.3% of all sites contained only native LDL, and 13.3% contained only OxLDL without monocyte/ macrophages. In contrast, only 4.3% of sites contained isolated monocytes in the absence of native or oxidized LDL. In addition, 6.3% of sites contained LDL and macrophages but few oxidation-specific epitopes. These results demonstrate that LDL oxidation and formation of fatty streaks occurs already during fetal development, and that both phenomena are greatly enhanced by maternal hypercholesterolemia. The fact that in very early lesions LDL and OxLDL are frequently found in the absence of monocyte/macrophages, whereas the opposite is rare, suggests that intimal LDL accumulation and oxidation contributes to monocyte recruitment in vivo.
975 citations
TL;DR: The data indicate that carrier-mediated transport may be essential for termination of the biological effects of anandamide, and may represent a potential drug target.
Abstract: Anandamide, an endogenous ligand for central cannabinoid receptors, is released from neurons on depolarization and rapidly inactivated. Anandamide inactivation is not completely understood, but it may occur by transport into cells or by enzymatic hydrolysis. The compound N-(4-hydroxyphenyl)arachidonylamide (AM404) was shown to inhibit high-affinity anandamide accumulation in rat neurons and astrocytes in vitro, an indication that this accumulation resulted from carrier-mediated transport. Although AM404 did not activate cannabinoid receptors or inhibit anandamide hydrolysis, it enhanced receptor-mediated anandamide responses in vitro and in vivo. The data indicate that carrier-mediated transport may be essential for termination of the biological effects of anandamide, and may represent a potential drug target.
820 citations
01 Jun 1997
TL;DR: A new task-priority redundancy resolution technique is developed that overcomes the effects of algorithmic singularities and is applied to a seven-degree-of-freedom manipulator in numerical case studies to demonstrate its effectiveness.
Abstract: Practical application of the task-priority redundancy resolution technique must deal with the occurrence of kinematic and algorithmic singularities. The aim of this paper is twofold. First, the application of existing singularity-robust methods to the case of kinematically redundant arms is studied. Then, a new task-priority redundancy resolution technique is developed that overcomes the effects of algorithmic singularities. Computational aspects of the solutions are also considered in view of real-time implementation of a kinematic control algorithm. The method is applied to a seven-degree-of-freedom manipulator in numerical case studies to demonstrate its effectiveness.
647 citations
TL;DR: Experimental results and sequence analysis suggest that an additional gene, males‐absent on the first (mof), encodes a putative acetyl transferase that plays a direct role in the specific histone acetylation associated with dosage compensation.
Abstract: Dosage compensation is a regulatory process that insures that males and females have equal amounts of X-chromosome gene products. In Drosophila, this is achieved by a 2-fold enhancement of X-linked gene transcription in males, relative to females. The enhancement of transcription is mediated by the activity of a group of regulatory genes characterized by the male-specific lethality of their loss-of-function alleles. The products of these genes form a complex that is preferentially associated with numerous sites on the X chromosome in somatic cells of males but not of females. Binding of the dosage compensation complex is correlated with a significant increase in the presence of a specific histone isoform, histone 4 acetylated at Lys16, on this chromosome. Experimental results and sequence analysis suggest that an additional gene, males-absent on the first (mof), encodes a putative acetyl transferase that plays a direct role in the specific histone acetylation associated with dosage compensation. The predicted amino acid sequence of MOF exhibits a significant level of similarity to several other proteins, including the human HIV-1 Tat interactive protein Tip60, the human monocytic leukemia zinc finger protein MOZ and the yeast silencing proteins SAS3 and SAS2.
443 citations
TL;DR: A short duration of obesity emerged as apotentially new risk factor of liver involvement in thepediatric obese population and needs to be confirmed infuture studies.
Abstract: Our aim was to evaluate incidence and riskfactors of liver involvement in obese Italian childrenas assessed by both ultrasonographic and biochemicalparameters. In seventy-five consecutive obese children (age 9.5 ± 2.9 years, males/females41/34), serum levels of enzymes and ultrasonography ofthe liver were evaluated. Tests were repeated one,three, and six months after starting a moderatehypocaloric diet and an exercise program. Three obese childrenwho were found to have chronic viral hepatitis wereexcluded from the study. Thirty-eight of 72 (53%) obesechildren had an ultrasonographic image of bright liver consistent with liver steatosis. Thelatter was severe in nine children, moderate in 16, andmild in 13. Eighteen obese children (25%) had elevatedtransaminase levels. Bright liver andhypertransaminasemia were not due to any of the most common causesof liver disease. Both were rapidly responsive to lossof weight, confirming that liver involvement wassecondary to obesity and that steatosis orsteatohepatitis rather than fibrosis were involved. Obesityduration not more than three years (odds ratio = 4.77),a higher degree of obesity (odds ratio = 2.09), andhypertransaminasemia (odds ratio = 2.15) appeared asimportant predictive factors of liver involvement atultrasonography. Incidence of liver involvement assessedby means of ultrasonography is significantly higher thanthat revealed by measurement of serum liver enzymes. A short duration of obesity emerged as apotentially new risk factor of liver involvement in thepediatric obese population and needs to be confirmed infuture studies.
400 citations
TL;DR: The relationship between tumor angiogenesis and survival for 407 patients with non-small-cell lung carcinoma who were treated with potentially curative surgery and in identifying subsets of patients who may benefit from different postsurgical treatments was evaluated.
Abstract: Background: Tumors acquire nutrients that are essential for continued growth and an avenue for dissemination to the rest of the body by inducing angiogenesis (i.e., the formation of new blood vessels). Preliminary studies involving a number of different kinds of cancer have indicated that an assessment of tumor angiogenesis may be useful in predicting disease outcome. Purpose: In a prospective study, we evaluated the relationship between tumor angiogenesis and survival for 407 patients with non-small-cell lung carcinoma who were treated with potentially curative surgery. Methods: The study population consisted of 360 male and 47 female patients who underwent surgery consecutively at the Department of Surgery, University of Pisa, Italy, from March 1991 through December 1994. Follow-up lasted through February 1996, with a median follow-up for living patients of 29 months (range, 15-60 months). An anti-CD34 monoclonal antibody, which is specific for endothelial cells, and standard immunohistochemical techniques were used to measure angiogenesis in tumor samples. Angiogenesis was quantified in terms of microvessel counts; the counts for single, high- power microscopic fields (magnification x250) in the three most intense areas of blood vessel growth for each sample were averaged. The median microvessel count in this series was 20, and the counts were categorized as follows : 1) low versus high (≤20 versus >20 microvessels) or 2) in five categories (1-10, 11-20, 21-30, 31-40, and ≥41 microvessels). Disease-free and overall survival during follow-up were assessed. Kaplan-Meier survival curves were modeled in a univariate analysis of patient and tumor characteristics; the Cox proportional hazards model was used in multivariate analysis. Reported P values are two-sided. Results and Conclusions: In the univariate analysis, patients with larger tumors (P for trend <.00001), a more advanced tumor stage (P for trend <.00001), a greater degree of regional lymph node involvement (P for trend <.00001), or more vascularized tumors (high versus low microvessel count, P<.00001) experienced significantly reduced overall survival. When microvessel counts were analyzed in five categories, a highly significant trend (P<.00001) toward worse prognosis was observed with increasing tumor vascularity. In multivariate analysis, tumor microvessel count (P<.00001), tumor size (P = .0006), and regional lymph node status (P<.00001) retained independent prognostic value with respect to overall survival; among these variables, tumor microvessel count, considered as a continuous variable, was the most important, with a relative hazard of death of 8.38 (95% confidence interval = 4.19-16.78) associated with the highest microvessel counts. Implications: An evaluation of tumor angiogenesis may be useful in the postsurgical staging of patients with non-small-cell lung carcinoma and in identifying subsets of patients who may benefit from different postsurgical treatments.
305 citations
TL;DR: Data indicate that the Rab7 protein in mammalian cells is present on a late endosomal compartment much larger than the compartment labeled by the cation-independent mannose 6-phosphate receptor, which appears to play a fundamental role in controlling late endocytic membrane traffic.
303 citations
TL;DR: It is suggested that a reduced intracellular magnesium concentration might be the missing link helping to explain the epidemiological association between NIDDM and hypertension.
298 citations
TL;DR: It is demonstrated that Sp3 is a bifunctional transcription factor that can both activate and repress transcription, and the repression activity is strictly dependent on the context of the DNA-binding sites bound by Sp3.
283 citations
TL;DR: This review focuses primarily on strategies aimed to elucidate the transcription properties of the Sp1-like factors and discusses the experimental problems inherent in the attempt to define their respective functions.
281 citations
TL;DR: Dietary intake of olive oil polyphenols may lower the risk of reactive oxygen metabolite-mediated diseases such as some gastrointestinal diseases and atherosclerosis, and the data demonstrate that (3,4-dihydroxyphenyl)ethanol can act as a biological antioxidant in a cell culture experimental model.
Abstract: We investigated the injurious effects of reactive oxygen metabolites on the intestinal epithelium and the possible protective role played by two olive oil phenolic compounds, (3,4-dihydroxyphenyl)ethanol and (p-hydroxyphenyl)ethanol, using the Caco-2 human cell line. We induced oxidative stress in the apical compartment, either by the addition of 10 mmol/L H2O2 or by the action of 10 U/L xanthine oxidase in the presence of xanthine (250 micromol/L); after the incubation, we evaluated the cellular and molecular alterations. Both treatments produced significant decreases in Caco-2 viability as assessed by the neutral red assay. Furthermore, we observed a significant increase in malondialdehyde intracellular concentration and paracellular inulin transport, indicating the occurrence of lipid peroxidation and monolayer permeability changes, respectively. The H2O2-induced alterations were completely prevented by preincubating Caco-2 cells with (3,4-dihydroxyphenyl)ethanol (250 micromol/L); when the oxidative stress was induced by xanthine oxidase, complete protection was obtained at a concentration of polyphenol as small as 100 micromol/L. In contrast, (p-hydroxyphenyl)ethanol was ineffective up to a concentration of 500 micromol/L. Our data demonstrate that (3,4-dihydroxyphenyl)ethanol can act as a biological antioxidant in a cell culture experimental model and that the ortho-dihydroxy moiety of the molecule is essential for antioxidant activity. This study suggests that dietary intake of olive oil polyphenols may lower the risk of reactive oxygen metabolite-mediated diseases such as some gastrointestinal diseases and atherosclerosis.
TL;DR: In this paper, a two-stage process is proposed for the thermal degradation of thin layers of milled, commercial xylan, where the first stage consists of fast sample heating (40-70 K s−1) to the desired temperature, which is then maintained until complete conversion.
TL;DR: The results show for the first time that NF‐κB is involved in COX‐2 protein expression in LPS‐stimulated J774 macrophages and suggest that inhibitors of NF‐kkB activation may represent a useful tool for the pharmacological control of inflammation.
TL;DR: The authentication and quality assessment of virgin olive oil were performed using high-resolution 1H and 13C NMR spectroscopy as mentioned in this paper, with emphasis on the detection of adulteration with foreign (seed) oils and esterified or refined olive and olive-pomace oils.
Abstract: The authentication and quality assessment of virgin olive oil were performed using high-resolution 1H and 13C NMR spectroscopy. An overview of the various determinations currently assessed is presented with emphasis on the detection of adulteration with foreign (seed) oils and esterified or refined olive and olive-pomace oils. Recent results on the NMR analysis of natural compounds (diacylglycerols, free fatty acids, aldehydes, polyphenols, etc.) related to the quality-freshness of virgin olive oil are also reported. The possible contribution of high-resolution NMR to the authentication of geographical origin of virgin olive oil is discussed. © 1997 John Wiley & Sons, Ltd.
TL;DR: The sequences in Mena that interact with the FE65 WW domain are identified and it is demonstrated that Mena binds to FE65 in vivo by coimmunoprecipitation assay from COS cell extracts and may help in understanding the mechanism of molecular changes that underlie Alzheimer disease.
TL;DR: In this article, isothermal melt crystallization of poly(L-lactide) (PLLA) has been studied in the temperature range of 90 to 135°C and a maximum in crystallization kinetic was observed around 105°C.
Abstract: Isothermal melt crystallization of poly(L-lactide) (PLLA) has been studied in the temperature range of 90 to 135°C. A maximum in crystallization kinetic was observed around 105°C. A transition from regime II to regime III is present around 115°C. The crystal morphology is a function of the degree of undercooling. At crystallization temperatures (Tc) below 105°C, further crystallization occurs upon heating; this behavior is not detected for Tc above 110°C. The analysis of the heat capacity increment at glass transition temperature (Tg) and of dielectric properties of PLLA indicates the presence of a fraction of the amorphous phase which does not relax at the Tg, and the amount of this so-called rigid amorphous phase is a function of Tc. © 1997 John Wiley & Sons, Inc. J Appl Polym Sci 64: 911–919, 1997
Journal Article•
TL;DR: Elevated FFA concentrations may play a pivotal role in the development of non-insulin-dependent diabetes mellitus and CHF was associated with a higher prevalence of NIDDM and was a risk factor for its development.
TL;DR: Results of this study show that local administration of 3′,5′-cyclic AMP and phosphodiesterase-inhibitor drugs to rats markedly inhibits neointima formation after balloon injury in vivo and in smooth muscle cells in vitro.
Abstract: Injury of the arterial wall induces the formation of the neointima. This structure is generated by the growth of mitogenically activated smooth muscle cells of the arterial wall. The molecular mechanism underlying the formation of the neointima involves deregulated cell growth, primarily triggered by the injury of the arterial wall. The activated gene products transmitting the injury-induced mitogenic stimuli have been identified and inhibited by several means: transdominant negative expression vectors, antisense oligodeoxynucleotides, adenovirus-mediated gene transfer, antibodies and inactivating drugs. Results of our study show that local administration of 3',5'-cyclic AMP and phosphodiesterase-inhibitor drugs (aminophylline and amrinone) to rats markedly inhibits neointima formation after balloon injury in vivo and in smooth muscle cells in vitro. The growth inhibitory effect of aminophylline was completely reversed by the inhibition of cAMP-dependent protein kinase A (PKA). These findings indicate an alternative approach to the treatment of diseases associated with injury-induced cell growth of the arterial wall, as stimulation of cAMP signaling is pharmacologically feasible in the clinical setting.
TL;DR: The present studies were performed to determine if a nitroxybutylester derivative of naproxen was less ulcerogenic to the gastrointestinal tract than its parent NSAID, and if it exerted comparable analgesic and anti‐inflammatory properties to the parentNSAID.
Abstract: Background: A novel class of nitric oxide-releasing nonsteroidal anti-inflammatory drug (NO-NSAID) derivatives has recently been described which exert anti-inflammatory activities but produce significantly less gastrointestinal injury than the parent NSAID from which they are derived. The present studies were performed to determine if a nitroxybutylester derivative of naproxen was less ulcerogenic to the gastrointestinal tract than its parent NSAID, and if it exerted comparable analgesic and anti-inflammatory properties to the parent NSAID.
Methods: The two drugs were compared in an acute gastric injury model, an antral ulcer model and after twice-daily administration for 18 days (small intestinal damage model). Anti-inflammatory activity was examined in the carrageenan-induced paw oedema model, while analgesia was examined in the acetic acid-induced writhing model. The pharmacokinetic profiles of naproxen vs. NO-naproxen were compared by HPLC analysis.
Results: NO-naproxen was found to produce significantly less gastric damage despite inducing similar increases in plasma TNFα to naproxen. With chronic administration, small intestinal damage was markedly less with NO-naproxen than with the parent NSAID. However, NO-naproxen exerted superior analgesic and comparable anti-inflammatory effects to naproxen. NO-naproxen was not completely converted to naproxen, but the reduced plasma levels of the latter was not the underlying reason for reduced gastrointestinal toxicity of NO-naproxen.
Conclusion: NO-naproxen represents a novel, gastrointestinal-sparing NSAID derivative with superior analgesic and comparable anti-inflammatory properties to naproxen.
TL;DR: It is suggested that the protection afforded by angina in adult patients may involve the occurrence of ischemic preconditioning, which seems to be lost in senescent patients.
TL;DR: It was shown that apo-lactoferrin hinders virus attachment to cell receptors since it is able to bind the viral particles and to prevent both rotavirus haemagglutination and viral binding to susceptible cells.
Abstract: Different milk proteins were analyzed for their inhibitory effect on either rotavirus-mediated agglutination of human erythrocytes or rotavirus infection of the human enterocyte-like cell line HT-29. Proteins investigated were α-lactalbumin, β-lactoglobulin, apo-lactoferrin, and Fe3+-lactoferrin, and their antiviral action was compared with the activity of mucin, a milk glycoprotein known to affect rotavirus infection. Results obtained demonstrated that β-lactoglobulin, apo- and Fe3+-lactoferrin are able to inhibit the replication of rotavirus in a dose-dependent manner, apo-lactoferrin being the most active. It was shown that apo-lactoferrin hinders virus attachment to cell receptors since it is able to bind the viral particles and to prevent both rotavirus haemagglutination and viral binding to susceptible cells. Moreover, this protein markedly inhibited rotavirus antigen synthesis and yield in HT-29 cells when added during the viral adsorption step or when it was present in the first hours of infection, suggesting that this protein interferes with the early phases of rotavirus infection.
TL;DR: The results indicate that activation of the NFκB complex by overexpression of p65 plays a critical role in the process of thyroid cell transformation.
Abstract: We have investigated the role of the NFκB complex in the process of thyroid carcinogenesis by analysing thyroid carcinoma cell lines. A significant increase in p65 NFκB mRNA and protein expression, compared to normal thyroid cultures or tissue, was found in all of the cancer cell lines. Conversely, only a modest increase in the p50 NFκB mRNA and protein was found in most, but not all carcinoma cell lines. The block of p65 protein synthesis with specific antisense oligonucleotides greatly reduced the ability of two undifferentiated carcinoma cell lines to form colonies in agar and reduced their growth rate. On the other hand, no effect was observed in the same cell lines when treated with p50 specific antisense oligonucleotides. These inhibitory effects seem to be mediated by the suppression of c-myc gene expression, since treatment with antisense oligonucleotides for p65 gene interfered negatively with c-myc gene expression. Our results indicate that activation of the NFκB complex by overexpression of p65 plays a critical role in the process of thyroid cell transformation.
TL;DR: It has been demonstrated that healthy centenarians have more favorable anthropometric characteristics and insulin-mediated glucose uptake than aged subjects and a more elevated plasma IGF-I/IGFBP-3 molar ratio might improve insulin action and plasma lipid concentration in centenarian.
Abstract: It has been demonstrated that healthy centenarians have more favorable anthropometric characteristics and insulin-mediated glucose uptake than aged subjects. The plasma insulin-like-growth factor I (IGF-I) concentration may account for such differences. Three groups of subjects were studied: 1) adults ( 100 yr; n = 19). In all subjects, fasting plasma IGF-I, IGF-binding protein-3 (IGFBP-3), leptin, and lipid concentrations were determined; body composition was assessed by bioimpedance analysis; and insulin-mediated glucose up-take was evaluated by euglycemic hyperinsulinemic glucose clamp. IGF-I declined with advancing age, but no differences between aged subjects and centenarians were found. IGFBP-3 showed a trend similar to IGF-I, but lower values were present in centenarians than in aged subjects. Nevertheless, centenarians had a plasma IGF-I/IGFBP-3 molar ratio greater than that in aged subjects. Centenarians had also a whole body glucose disposal (WBGD) greater than that in aged subjects, but similar to that in adults. Mini Mental State Examination (27 +/- 2.1 vs. 18.3 +/- 3.1; P < 0.02) and Instrumental Activities Daily Living (26 +/- 2.6 vs. 8.4 +/- 4.1; P < 0.001) scores were significantly different in aged subjects and centenarians, respectively. In centenarians, the plasma IGF-I/IGFBP-3 molar ratio correlated with the body mass index (r = -0.55; P < 0.009); the amount of body fat (r = -0.62; P < 0.003); fat-free mass (r = 0.56; P < 0.008); fasting plasma leptin (r = -0.63; P < 0.004), triglycerides (r = -0.58; P < 0.01), free fatty acid (r = -0.64; P < 0.005), and low density lipoprotein cholesterol (r = -0.59; P < 0.009) concentrations; Mini Mental State Examination (r = 0.53; P < 0.0.03); and WBGD (r = 0.64; P < 0.005). All correlations were independent of daily fat and carbohydrate intake and WBGD (P < 0.05 for all). No significant correlations between the plasma IGF-I/IGFBP-3 molar ratio and plasma total (r = 0.31; P = NS) and high density lipoprotein cholesterol (r = 0.34; P = NS) concentrations were present. The correlation between the plasma IGF-I/IGFBP-3 molar ratio and WBGD persisted after adjustment for body fat, fasting plasma insulin concentration, daily carbohydrate and fat intake, and daily physical activity (r = 0.55; P < 0.009), but not after further adjustment for plasma free fatty acid concentration (r = 0.30; P = 0.17). In conclusion, healthy centenarians have plasma IGF-I/IGFBP-3 molar ratio greater than aged subjects. A more elevated plasma IGF-I/IGFBP-3 molar ratio might improve insulin action and plasma lipid concentration in centenarians.
TL;DR: Some procedural aspects of PEI treatment differ among the various centers a standardization is advisable, and in the present survey PEI is a low-risk technique.
Abstract: Background: Percutaneous ethanol injection (PEI) has become a widely used procedure in the treatment of hepatocellular carcinoma (HCC). However, the criteria for selecting patients are not standardized, and little information is available about the complications of the procedure. Methods: A questionnaire was sent to 11 experienced Italian centers. It investigated: the size and the number of HCC nodules suitable for treatment and the Child-Pugh risk class of the associated cirrhosis; the performance of the procedure; the number and characteristics of the patients treated; and, finally, any complications. Results: Most of the centers performed PEI in single HCC nodules less than 5 cm in diameter or in multiple nodules if fewer than three, the larger being less than 3 cm. Patients in Child-Pugh's classes A, B, and C with single nodules were generally considered for PEI. A prothrombin time of less than 40% and a platelet count of less than 40, 000/mm3 contraindicated PEI in most of the centers. PEI was genera...
TL;DR: The capacity of MTB to induce apoptosis in cells obtained by broncho‐alveolar lavage from patients with reactive pulmonary tuberculosis and from AIDS patients with disseminated pulmonary tuberculosis is analyzed.
Abstract: Tuberculosis (TB) is still a major health problem, both as a single disease entity and as a cofactor in AIDS. The interaction between macrophage and Mycobacterium tuberculosis (MTB) is a critical step in the establishment of an early chronic infection. This study analyses the capacity of MTB to induce apoptosis in cells obtained by broncho-alveolar lavage (BAL) from patients with reactive pulmonary tuberculosis and from AIDS patients with disseminated pulmonary tuberculosis. Apoptosis was increased three-fold in BAL cells obtained from patients with pulmonary tuberculosis and even more markedly in alveolar macrophages of MTB-infected AIDS patients, compared with controls. Apoptosis was analysed and characterized by propidium iodide (PI) incorporation, terminal deoxy transferase (TDT)-mediated dUTP-biotin nick end labelling (TUNEL), and tissue transglutaminase (tTG) expression. The MTB–macrophage interaction was also investigated in vitro by infecting monocyte-derived macrophages (MDM) with MTB (virulent strain H37Rv). The induction of apoptosis by MTB required viable bacteria, was dose-dependent, and was restricted to H37Rv. Infection with either Mycobacterium avium complex (MAC) or HIV-1 and treatment with heat-killed MTB failed to induce apoptosis. © 1997 by John Wiley & Sons, Ltd.
TL;DR: A role for Fe65 in the pathogenesis of familial Alzheimer's disease is suggested by the finding that mutant APP shows an altered in vivo interaction with Fe65, as demonstrated by anti-Fe65 co-immunoprecipitation experiments.
TL;DR: In this paper, a novel silicon optical amplitude-phase modulator integrated into a SOI (silicon on insulator) optical waveguide and based on a three terminal electronic structure was analyzed.
Abstract: We analyze, from a theoretical point of view, a novel silicon optical amplitude-phase modulator integrated into a SOI (silicon on insulator) optical waveguide and based on a three terminal electronic structure which gives rise to definite advantages in comparison with a classical p-i-n diode based modulator. The proposed device utilizes the free carrier dispersion effect to produce the desired refractive index and absorption coefficient variations. The MEDICI two-dimensional (2-D) semiconductor device simulator has been used to analyze the electrical operation, with reference to the free carrier concentration injected into the optical channel, its uniformity and the required current density and electrical power. The optical investigation was carried out by means of FDM (finite difference method), EIM (effective index method), and BPM (beam propagation method) tools, giving rise to a complete evaluation of the properties of our device. We report the results for both the amplitude and phase modulators, paying attention to the static and the dynamic behavior. In particular, an amplitude modulation of 20%, with an injection power of about 126 mW, and a switching time of 5.6 ns can be achieved theoretically, Furthermore, as a phase modulator, the device exhibits a very high figure of merit, predicting an induced phase shift per volt per millimeter of about 215/spl deg/, for a injection power of about 43 mW, and a switching time shorter than 3.5 ns.
TL;DR: The Italian production of agricultural residues has been evaluated with a view to energy recovery through gasification as mentioned in this paper, and two main categories of residues have been identified: the first, (A) is associated with the growing and collection of products with a nutritional value, whereas the second (B) includes the residues associated with subsequent processing in order to obtain final products for commercialization.
Abstract: The Italian production of agricultural residues has been evaluated with a view to energy recovery through gasification. Two main categories of residues have been identified: the first, (A) is associated with the growing and collection of products with a nutritional value, whereas the second (B) includes the residues associated with the subsequent processing in order to obtain final products for commercialization. Category A, which comprises three further sub-categories: straw (A1); woody residues (A2); and stems and leaves (residues from vegetables, tobacco, sugar beet, (A3)), results in about 16.5 mt/yr. The average amount of straw (A1) is 11 mt/yr, of which about 60% is waste to be eliminated. Woody residues (A2) (mainly pruning off-cuts from vineyards and olive groves) are about 3.5 mt/yr (85% unused). Category A3 amounts to about 2 mt/yr (90% unused). Straw is available mainly in the northern part of the country, whereas the other two sub-categories are widely distributed in central and southern regions. The yields of category B are estimated at 4 mt/yr, of which more than 3 mt/yr are waste products from grape and olive processing. Other residues, such as rice, sunflower and soya-bean husks (about 0.65 mt/yr), almond and nut shells and fruit stones (about 0.2 mt/yr), although not widely available on a national scale, can be significant on a local basis. The total amount of unused agricultural residues is about 14.5 mt/yr, which, if completly exploited through gasification, can contribute as much as 7–10% to the current national electricity needs. The regions of Veneto, Puglia, Friuli, Lombardia and Emilia Romagna appear to be good candidates for electricity production, given the significant surface concentration of unused residues (105-55 t/km2).
TL;DR: In this article, a new method for designing moment resisting steel frames failing in a global mode is presented, which is based on the application of the kinematic theorem of plastic collapse.
Abstract: A new method for designing moment resisting steel frames failing in a global mode is presented in this paper. Starting from the analysis of the typical collapse mechanisms of frames subjected to horizontal forces, the method is based on the application of the kinematic theorem of plastic collapse. The beam section properties are assumed to be known quantities, because they are designed to resist vertical loads. As a consequence, the unknowns of the design problem are the column sections. They are determined by means of design conditions expressing that the kinematically admissible multiplier of the horizontal forces corresponding to the global mechanism has to be the smallest among all kinematically admissible multipliers.
In addition, the proposed design method includes both the influence of distributed loads acting on the beams and the influence of second-order effects. In particular, the influence of second-order effects, which can play an important role in the seismic design of steel frames, is accounted for by the mechanism equilibrium curves of the analysed collapse mechanisms.
Moreover, in order to show the practical application of the proposed design procedure, a worked example is presented. Finally, the inelastic behaviour of the designed frame is compared to that obtained when the simple member hierarchy criterion or a similar rule is applied. © 1997 by John Wiley & Sons, Ltd.