Institution
University of Nevada, Reno
Education•Reno, Nevada, United States•
About: University of Nevada, Reno is a education organization based out in Reno, Nevada, United States. It is known for research contribution in the topics: Population & Poison control. The organization has 13561 authors who have published 28217 publications receiving 882002 citations. The organization is also known as: University of Nevada & Nevada State University.
Papers published on a yearly basis
Papers
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TL;DR: It is suggested that the plasmalemma, the acrosome, and the mitochondria of unfrozen spermatozoa varied as to their functional status, while the cryopreservation process resulted in a more uniform status of sperm organelles.
Abstract: Flow cytometry was used to compare the functional status of fluorescently stained sperm organelles from 12 Holstein bulls after storage for 24 h at 5 degrees C and after cryopreservation. The organelle-specific stains, SYBR-14 and LysoTracker Green DND-26, identified spermatozoa with intact plasmalemma and those with intact acrosomes, respectively. The mitochondria-specific stain, 5,5',6,6'-tetrachloro-1,1',3,3'-tetraethylbenzimidazolyl-carbocyan ine iodide (JC-1), identified two populations of spermatozoa. One population stained red-orange because the JC-1 accumulated in the mitochondria as aggregates (characteristic of cells exhibiting a high membrane potential); a second population stained green because of JC-1 monomers within the mitochondria (characteristic of cells exhibiting a lower membrane potential). Analysis of variance revealed that within bulls, the properties of sperm viability, intact acrosomes, and mitochondrial status differed in spermatozoa stored for 24 h (p 0.11). Linear regression analyses resulted in significant models in which the proportions of stained spermatozoa stored for 24 h were indicative of those proportions observed in the cryopreserved fractions. These findings suggest that the plasmalemma, the acrosome, and the mitochondria of unfrozen spermatozoa varied as to their functional status. The cryopreservation process, however, resulted in a more uniform status of sperm organelles.
183 citations
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TL;DR: The reaction kinetics and effects of particle size on HTC were investigated and a simple reaction mechanism is proposed and validated, in which both hemicellulose and cellulose degrade in parallel first-order reactions.
183 citations
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TL;DR: A novel mouse with bright green fluorescent protein expressed specifically in the fibroblast‐like cells was used to identify these cells in the mixture of cells obtained when whole muscles are dispersed with enzymes, and they responded to a major class of inhibitory neurotransmitters – purines.
Abstract: Non-technical summary
Smooth muscles, as in the gastrointestinal tract, are composed of several types of cells. Gastrointestinal muscles contain smooth muscle cells, enteric neurons, glial cells, immune cells, and various classes of interstitial cells. One type of interstitial cell, referred to as ‘fibroblast-like cells’ by morphologists, are common, but their function is unknown. These cells are found near the terminals of enteric motor neurons, suggesting they could have a role in generating neural responses that help control gastrointestinal movements. We used a novel mouse with bright green fluorescent protein expressed specifically in the fibroblast-like cells to help us identify these cells in the mixture of cells obtained when whole muscles are dispersed with enzymes. We isolated these cells and found they respond to a major class of inhibitory neurotransmitters – purines. We characterized these responses, and our results provide a new hypothesis about the role of fibroblast-like cells in smooth muscle tissues.
183 citations
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TL;DR: The status of the Zika virus outbreak is reviewed, with a focus on how the epidemiology, insect vectors, neuropathology, virology and immunology inform options and strategies available for medical countermeasure development and deployment.
Abstract: Introduction Reports of high rates of primary microcephaly and Guillain-Barre syndrome associated with Zika virus infection in French Polynesia and Brazil have raised concerns that the virus circulating in these regions is a rapidly developing neuropathic, teratogenic, emerging infectious public health threat. There are no licensed medical countermeasures (vaccines, therapies or preventive drugs) available for Zika virus infection and disease. The Pan American Health Organization (PAHO) predicts that Zika virus will continue to spread and eventually reach all countries and territories in the Americas with endemic Aedes mosquitoes. This paper reviews the status of the Zika virus outbreak, including medical countermeasure options, with a focus on how the epidemiology, insect vectors, neuropathology, virology and immunology inform options and strategies available for medical countermeasure development and deployment. Methods Multiple information sources were employed to support the review. These included publically available literature, patents, official communications, English and Lusophone lay press. Online surveys were distributed to physicians in the US, Mexico and Argentina and responses analyzed. Computational epitope analysis as well as infectious disease outbreak modeling and forecasting were implemented. Field observations in Brazil were compiled and interviews conducted with public health officials.
183 citations
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TL;DR: The recent progress made in understanding the biogenesis and regulation of circRNAs are highlighted, newly uncovered circRNA functions are discussed, and the methodological approaches that could reveal more exciting and unexpected roles for these RNAs are explained.
Abstract: Many thousands of Circular RNAs (circRNAs) have recently been identified in metazoan genomes by transcriptome-wide sequencing. Most circRNAs are generated by back-splicing events from exons of protein-coding genes. A great deal of progress has recently been made in understanding the genome-wide expression patterns, biogenesis, and regulation of circRNAs. To date, however, few functions of circRNAs have been identified. CircRNAs are preferentially expressed in neural tissues and some are found at synapses, suggesting possible functions in the nervous system. Several circRNAs have been shown to function as microRNA "sponges" to counteract microRNA mediated repression of mRNA. New functions for circRNAs are arising, including protein sequestration, transcriptional regulation, and potential functions in cancer. Here, we highlight the recent progress made in understanding the biogenesis and regulation of circRNAs, discuss newly uncovered circRNA functions, and explain the methodological approaches that could reveal more exciting and unexpected roles for these RNAs.
183 citations
Authors
Showing all 13726 results
Name | H-index | Papers | Citations |
---|---|---|---|
Robert Langer | 281 | 2324 | 326306 |
Thomas C. Südhof | 191 | 653 | 118007 |
David W. Johnson | 160 | 2714 | 140778 |
Menachem Elimelech | 157 | 547 | 95285 |
Jeffrey L. Cummings | 148 | 833 | 116067 |
Bing Zhang | 121 | 1194 | 56980 |
Arturo Casadevall | 120 | 980 | 55001 |
Mark H. Ellisman | 117 | 637 | 55289 |
Thomas G. Ksiazek | 113 | 398 | 46108 |
Anthony G. Fane | 112 | 565 | 40904 |
Leonardo M. Fabbri | 109 | 566 | 60838 |
Gary H. Lyman | 108 | 694 | 52469 |
Steven C. Hayes | 106 | 450 | 51556 |
Stephen P. Long | 103 | 384 | 46119 |
Gary Cutter | 103 | 737 | 40507 |