University of Oviedo

About: University of Oviedo is a education organization based out in Oviedo, Spain. It is known for research contribution in the topics: Population & Large Hadron Collider. The organization has 13423 authors who have published 31649 publications receiving 844799 citations. The organization is also known as: Universidá d'Uviéu & Universidad de Oviedo.

A decision support system for applying failure mode and effects analysis

Abstract: This study describes an alternative way of applying failure mode and effects analysis (FMEA) to a wide variety of problems. It presents a methodology based on a decision system supported by qualitative rules which provides a ranking of the risks of potential causes of production system failures. By providing an illustrative example, it highlights the advantages of this flexible system over the traditional FMEA model. Finally, a fuzzy decision model is proposed, which improves the initial decision system by introducing the element of uncertainty.

Barriers to the Implementation of Environmentally Oriented Reverse Logistics: Evidence from the Automotive Industry Sector

Abstract: Even though reverse logistics has existed as long as forward logistics, growing social concern for the environment has caused reverse logistics activities to become a critical function for many organizations. Yet, barriers to implementation of environmentally oriented aspects of reverse logistics still exist. The aim of the present study is to identify the barriers that impede or hinder the implementation of environmentally oriented reverse logistics practices, with a focus on the automotive industry. Empirical evidence and robust statistical analysis provide insights into the practices of environmentally oriented reverse logistics and barriers encountered in the Spanish automotive sector. Using structural equation modelling we are able identify two types of barriers, external and internal, and to study whether these barriers, previously identified in the literature, hinder environmentally oriented reverse logistics practices. The model also allows for identification of the most relevant barriers for the automotive sector.

Activation and identification of five clusters for secondary metabolites in Streptomyces albus J1074

Abstract: Summary Streptomyces albus J1074 is a streptomycete strain widely used as a host for expression of secondary metabolite gene clusters. Bioinformatic analysis of the genome of this organism predicts the presence of 27 gene clusters for secondary metabolites. We have used three different strategies for the activation of some of these silent/cryptic gene clusters in S. albus J1074: two hybrid polyketide-non-ribosomal peptides (PK-NRP) (antimycin and 6-epi-alteramides), a type I PK (candicidin), a non-ribosomal peptides (NRP) (indigoidine) and glycosylated compounds (paulomycins). By insertion of a strong and constitutive promoter in front of selected genes of two clusters, production of the blue pigment indigoidine and of two novel members of the polycyclic tetramate macrolactam family (6-epi-alteramides A and B) was activated. Overexpression of positive regulatory genes from the same organism also activated the biosynthesis of 6-epi-alteramides and heterologous expression of the regulatory gene pimM of the pimaricin cluster activated the simultaneous production of candicidins and antimycins, suggesting some kind of cross-regulation between both clusters. A cluster for glycosylated compounds (paulomycins) was also identified by comparison of the high-performance liquid chromatography profiles of the wild-type strain with that of a mutant in which two key enzymes of the cluster were simultaneously deleted.