University of Oviedo

About: University of Oviedo is a education organization based out in Oviedo, Spain. It is known for research contribution in the topics: Population & Large Hadron Collider. The organization has 13423 authors who have published 31649 publications receiving 844799 citations. The organization is also known as: Universidá d'Uviéu & Universidad de Oviedo.

Aberrant DNA methylation patterns of spermatozoa in men with unexplained infertility

Abstract: STUDY QUESTION Are there DNA methylation alterations in sperm that could explain the reduced biological fertility of male partners from couples with unexplained infertility? SUMMARY ANSWER DNA methylation patterns, not only at specific loci but also at Alu Yb8 repetitive sequences, are altered in infertile individuals compared with fertile controls. WHAT IS KNOWN ALREADY Aberrant DNA methylation of sperm has been associated with human male infertility in patients demonstrating either deficiencies in the process of spermatogenesis or low semen quality. STUDY DESIGN, SIZE, DURATION Case and control prospective study. This study compares 46 sperm samples obtained from 17 normospermic fertile men and 29 normospermic infertile patients. PARTICIPANTS/MATERIALS, SETTING, METHODS Illumina Infinium HD Human Methylation 450K arrays were used to identify genomic regions showing differences in sperm DNA methylation patterns between five fertile and seven infertile individuals. Additionally, global DNA methylation of sperm was measured using the Methylamp Global DNA Methylation Quantification Ultra kit (Epigentek) in 14 samples, and DNA methylation at several repetitive sequences (LINE-1, Alu Yb8, NBL2, D4Z4) measured by bisulfite pyrosequencing in 44 sperm samples. A sperm-specific DNA methylation pattern was obtained by comparing the sperm methylomes with the DNA methylomes of differentiated somatic cells using data obtained from methylation arrays (Illumina 450 K) of blood, neural and glial cells deposited in public databases. MAIN RESULTS AND THE ROLE OF CHANCE In this study we conduct, for the first time, a genome-wide study to identify alterations of sperm DNA methylation in individuals with unexplained infertility that may account for the differences in their biological fertility compared with fertile individuals. We have identified 2752 CpGs showing aberrant DNA methylation patterns, and more importantly, these differentially methylated CpGs were significantly associated with CpG sites which are specifically methylated in sperm when compared with somatic cells. We also found statistically significant (P < 0.001) associations between DNA hypomethylation and regions corresponding to those which, in somatic cells, are enriched in the repressive histone mark H3K9me3, and between DNA hypermethylation and regions enriched in H3K4me1 and CTCF, suggesting that the relationship between chromatin context and aberrant DNA methylation of sperm in infertile men could be locus-dependent. Finally, we also show that DNA methylation patterns, not only at specific loci but also at several repetitive sequences (LINE-1, Alu Yb8, NBL2, D4Z4), were lower in sperm than in somatic cells. Interestingly, sperm samples at Alu Yb8 repetitive sequences of infertile patients showed significantly lower DNA methylation levels than controls. LIMITATIONS, REASONS FOR CAUTION Our results are descriptive and further studies would be needed to elucidate the functional effects of aberrant DNA methylation on male fertility. WIDER IMPLICATIONS OF THE FINDINGS Overall, our data suggest that aberrant sperm DNA methylation might contribute to fertility impairment in couples with unexplained infertility and they provide a promising basis for future research. STUDY FUNDING/COMPETING INTERESTS This work has been financially supported by Fundacion Cientifica de la AECC (to R.G.U.); IUOPA (to G.F.B.); FICYT (to E.G.T.); the Spanish National Research Council (CSIC; 200820I172 to M.F.F.); Fundacion Ramon Areces (to M.F.F); the Plan Nacional de I+D+I 2008-2011/2013-2016/FEDER (PI11/01728 to AF.F., PI12/01080 to M.F.F. and PI12/00361 to S.L.); the PN de I+D+I 2008-20011 and the Generalitat de Catalunya (2009SGR01490). A.F.F. is sponsored by ISCIII-Subdireccion General de Evaluacion y Fomento de la Investigacion (CP11/00131). S.L. is sponsored by the Researchers Stabilization Program from the Spanish National Health System (CES09/020). The IUOPA is supported by the Obra Social Cajastur, Spain.

Family Ownership and Control, the Presence of Other Large Shareholders, and Firm Performance: Further Evidence:

Abstract: This article analyzes, using various econometric techniques, how family ownership, family control, and the presence of a second significant shareholder affect firm performance. The authors studied a panel of 118 nonfinancial Spanish companies (711 observations) from 2002 to 2008. Once endogeneity issues were considered, it was found that family ownership did not influence profitability. What seems to matter is family control. This study also reveals the importance of taking into account unobservable heterogeneity and endogeneity issues when analyzing firm performance and provides an interesting future avenue of research: the role played by other large shareholders in family firms.

Planck early results. XXI. Properties of the interstellar medium in the Galactic plane

Abstract: Planck has observed the entire sky from 30 GHz to 857GHz. The observed foreground emission contains contributions from different phases of the interstellar medium (ISM). We have separated the observed Galactic emission into the different gaseous components (atomic, molecular and ionised) in each of a number of Galactocentric rings. This technique provides the necessary information to study dust properties (emissivity, temperature, etc.), as well as other emission mechanisms as a function of Galactic radius. Templates are created for various Galactocentric radii using velocity information from atomic (neutral hydrogen) and molecular ((12)CO) observations. The ionised template is assumed to be traced by free-free emission as observed by WMAP, while 408 MHz emission is used to trace the synchrotron component. Gas emission not traced by the above templates, namely “dark gas”, as evidenced using Planck data, is included as an additional template, the first time such a component has been used in this way. These templates are then correlated with each of the Planck frequency bands, as well as with higher frequency data from IRAS and DIRBE along with radio data at 1.4 GHz. The emission per column density of the gas templates allows us to create distinct spectral energy distributions (SEDs) per Galactocentric ring and in each of the gaseous tracers from 1.4 GHz to 25 THz (12μm). The resulting SEDs allow us to explore the contribution of various emission mechanisms to the Planck signal. Apart from the thermal dust and free-free emission, we have probed the Galaxy for anomalous (e.g., spinning) dust as well as synchrotron emission. We find the dust opacity in the solar neighbourhood, τ/N(H) = 0.92 ± 0.05 × 10(-25) cm(2) at 250 μm, with no significant variation with Galactic radius, even though the dust temperature is seen to vary from over 25 K to under 14 K. Furthermore, we show that anomalous dust emission is present in the atomic, molecular and dark gas phases throughout the Galactic disk. Anomalous emission is not clearly detected in the ionised phase, as free-free emission is seen to dominate. The derived dust propeties associated with the dark gas phase are derived but do not allow us to reveal the nature of this phase. For all environments, the anomalous emission is consistent with rotation from polycyclic aromatic hydrocarbons (PAHs) and, according to our simple model, accounts for (25 ± 5)% (statistical) of the total emission at 30 GHz.