University of Palermo

About: University of Palermo is a education organization based out in Palermo, Italy. It is known for research contribution in the topics: Population & Cancer. The organization has 15621 authors who have published 40250 publications receiving 964384 citations. The organization is also known as: Università degli Studi di Palermo & Universita degli Studi di Palermo.

Usefulness of alpha-fetoprotein in the diagnosis of hepatocellular carcinoma.

Abstract: With the widespread use of ultrasonography (US) and computerized tomography (CT), the usefulness of alpha-fetoprotein assay in the diagnosis of hepatocellular carcinoma (HCC) has decreased. The aim of our study was to evaluate the best cut-off value for serum alpha-fetoprotein to discriminate between liver cirrhosis (LC) and HCC and the factors influencing levels in a Sicilian population. Three hundred and seventy-two patients with LC and 197 with HCC-associated LC were studied. The etiology was: HCV in 288 cases (77.4%) of LC and 147 cases (75%) of HCC; HBV in 31 cases (8.3%) of LC and 15 cases (7.6%) of HCC; HCV/HBV in 21 cases (5.6%) of LC and 6 cases (3.0%) of HCC; non-viral in 32 cases (8.6%) of LC and 29 cases (15%) of HCC. Hepatic function was estimated by the Child-Pugh's score; the TNM classification was used in HCC. The area under the ROC curve was 0.81 +/- 0.02; the best discriminant cut-off value, calculated as the value of the maximized likelihood ratio, was 30 ng/ml. At this level sensitivity (SE) was 65%, specificity (SP) 89%, positive predictive value (PPV) 74% and negative predictive value (NPV) 79%. When the patients were divided at this cut-off point into two groups according to viral or non-viral etiology, PPV was 70% versus 94%, respectively (p 30 ng/ml correlated positively only with the TNM stage (p < 0.0001). In conclusion, the best cut-off value for serum AFP in our study population was 30 ng/ml, but at this level sensitivity was low. This cut-off value was more useful in detecting non-viral HCC, because PPV was significantly higher than in viral HCC; therefore, our data confirm that the usefulness of AFP in the diagnosis of HCC of viral etiology is limited, being more useful in HCC of non-viral etiology.

Therapeutic resistance resulting from mutations in Raf/MEK/ERK and PI3K/PTEN/Akt/mTOR signaling pathways.

Abstract: Chemotherapy remains a commonly used therapeutic approach for many cancers. Indeed chemotherapy is relatively effective for treatment of certain cancers and it may be the only therapy (besides radiotherapy) that is appropriate for certain cancers. However, a common problem with chemotherapy is the development of drug resistance. Many studies on the mechanisms of drug resistance concentrated on the expression of membrane transporters and how they could be aberrantly regulated in drug resistant cells. Attempts were made to isolate specific inhibitors which could be used to treat drug resistant patients. Unfortunately most of these drug transporter inhibitors have not proven effective for therapy. Recently the possibilities of more specific, targeted therapies have sparked the interest of clinical and basic researchers as approaches to kill cancer cells. However, there are also problems associated with these targeted therapies. Two key signaling pathways involved in the regulation of cell growth are the Ras/Raf/MEK/ERK and PI3K/PTEN/Akt/mTOR pathways. Dysregulated signaling through these pathways is often the result of genetic alterations in critical components in these pathways as well as mutations in upstream growth factor receptors. Furthermore, these pathways may be activated by chemotherapeutic drugs and ionizing radiation. This review documents how their abnormal expression can contribute to drug resistance as well as resistance to targeted therapy. This review will discuss in detail PTEN regulation as this is a critical tumor suppressor gene frequently dysregulated in human cancer which contributes to therapy resistance. Controlling the expression of these pathways could improve cancer therapy and ameliorate human health.

Chandra X-Ray Observation of the Orion Nebula Cluster. II. Relationship between X-Ray Activity Indicators and Stellar Parameters

Abstract: Using the results of our first paper on the Chandra HRC observation of the Orion Nebula cluster (ONC), we explore the relation between the coronal activity of its 1 Myr-old pre-main-sequence population and stellar parameters. We find that median X-ray luminosities of low-mass stars (M/M☉ 3) increase with increasing mass and decreasing stellar age. Brown dwarfs (0.03 M/M☉ 0.08) follow the same trend with mass. From M ~ 0.1 to M ~ 0.5 M☉, median LX/Lbol values increase by about half an order of magnitude and then remain constant at ~10-3.5 for the mass range from 0.5 to 3.0 M/M☉. In these same two mass ranges, LX/Lbol remains roughly constant with age, until it drops by more than 2 orders of magnitudes at the epoch when ~2-4 M☉ stars are expected to become fully radiative. We find a dependence of LX and LX/Lbol on circumstellar accretion indicators and suggest three possible hypotheses for its origin. In spite of improved X-ray and rotational data, correlations between activity indicators and rotation remain elusive for these stars, possibly indicating that stars for which rotational periods have been measured have reached some saturation level. Our study of X-ray activity versus stellar mass leads us to propose that the few HRC X-ray sources not associated with any optical/infrared counterpart trace a yet-to-be-discovered stellar population of deeply embedded, relatively massive ONC members.