University of Palermo

About: University of Palermo is a education organization based out in Palermo, Italy. It is known for research contribution in the topics: Population & Cancer. The organization has 15621 authors who have published 40250 publications receiving 964384 citations. The organization is also known as: Università degli Studi di Palermo & Universita degli Studi di Palermo.

Interventions to Slow Aging in Humans: Are We Ready?

Abstract: The workshop entitled 'Interventions to Slow Aging in Humans: Are We Ready?' was held in Erice, Italy, on October 8-13, 2013, to bring together leading experts in the biology and genetics of aging and obtain a consensus related to the discovery and development of safe interventions to slow aging and increase healthy lifespan in humans. There was consensus that there is sufficient evidence that aging interventions will delay and prevent disease onset for many chronic conditions of adult and old age. Essential pathways have been identified, and behavioral, dietary, and pharmacologic approaches have emerged. Although many gene targets and drugs were discussed and there was not complete consensus about all interventions, the participants selected a subset of the most promising strategies that could be tested in humans for their effects on healthspan. These were: (i) dietary interventions mimicking chronic dietary restriction (periodic fasting mimicking diets, protein restriction, etc.); (ii) drugs that inhibit the growth hormone/IGF-I axis; (iii) drugs that inhibit the mTOR-S6K pathway; or (iv) drugs that activate AMPK or specific sirtuins. These choices were based in part on consistent evidence for the pro-longevity effects and ability of these interventions to prevent or delay multiple age-related diseases and improve healthspan in simple model organisms and rodents and their potential to be safe and effective in extending human healthspan. The authors of this manuscript were speakers and discussants invited to the workshop. The following summary highlights the major points addressed and the conclusions of the meeting.

Mapping daily evapotranspiration at field to continental scales using geostationary and polar orbiting satellite imagery

Abstract: . Thermal infrared (TIR) remote sensing of land-surface temperature (LST) provides valuable information about the sub-surface moisture status required for estimating evapotranspiration (ET) and detecting the onset and severity of drought. While empirical indices measuring anomalies in LST and vegetation amount (e.g., as quantified by the Normalized Difference Vegetation Index; NDVI) have demonstrated utility in monitoring ET and drought conditions over large areas, they may provide ambiguous results when other factors (e.g., air temperature, advection) are affecting plant functioning. A more physically based interpretation of LST and NDVI and their relationship to sub-surface moisture conditions can be obtained with a surface energy balance model driven by TIR remote sensing. The Atmosphere-Land Exchange Inverse (ALEXI) model is a multi-sensor TIR approach to ET mapping, coupling a two-source (soil + canopy) land-surface model with an atmospheric boundary layer model in time-differencing mode to routinely and robustly map daily fluxes at continental scales and 5 to 10-km resolution using thermal band imagery and insolation estimates from geostationary satellites. A related algorithm (DisALEXI) spatially disaggregates ALEXI fluxes down to finer spatial scales using moderate resolution TIR imagery from polar orbiting satellites. An overview of this modeling approach is presented, along with strategies for fusing information from multiple satellite platforms and wavebands to map daily ET down to resolutions on the order of 10 m. The ALEXI/DisALEXI model has potential for global applications by integrating data from multiple geostationary meteorological satellite systems, such as the US Geostationary Operational Environmental Satellites, the European Meteosat satellites, the Chinese Fen-yung 2B series, and the Japanese Geostationary Meteorological Satellites. Work is underway to further evaluate multi-scale ALEXI implementations over the US, Europe, Africa and other continents with geostationary satellite coverage.

Guidelines for the use of flow cytometry and cell sorting in immunological studies

Abstract: The marriage between immunology and cytometry is one of the most stable and productive in the recent history of science. A rapid search in PubMed shows that, as of July 2017, using “flow cytometry immunology” as a search term yields more than 68 000 articles, the first of which, interestingly, is not about lymphocytes. It might be stated that, after a short engagement, the exchange of the wedding rings between immunology and cytometry officially occurred when the idea to link fluorochromes to monoclonal antibodies came about. After this, recognizing different types of cells became relatively easy and feasible not only by using a simple fluorescence microscope, but also by a complex and sometimes esoteric instrument, the flow cytometer that is able to count hundreds of cells in a single second, and can provide repetitive results in a tireless manner. Given this, the possibility to analyse immune phenotypes in a variety of clinical conditions has changed the use of the flow cytometer, which was incidentally invented in the late 1960s to measure cellular DNA by using intercalating dyes, such as ethidium bromide. The epidemics of HIV/AIDS in the 1980s then gave a dramatic impulse to the technology of counting specific cells, since it became clear that the quantification of the number of peripheral blood CD4+ T cells was crucial to follow the course of the infection, and eventually for monitoring the therapy. As a consequence, the development of flow cytometers that had to be easy-to-use in all clinical laboratories helped to widely disseminate this technology. Nowadays, it is rare to find an immunological paper or read a conference abstract in which the authors did not use flow cytometry as the main tool to dissect the immune system and identify its fine and complex functions. Of note, recent developments have created the sophisticated technology of mass cytometry, which is able to simultaneously identify dozens of molecules at the single cell level and allows us to better understand the complexity and beauty of the immune system.