Showing papers by "University of Reading published in 2001"

PESA-II: region-based selection in evolutionary multiobjective optimization

Abstract: We describe a new selection technique for evolutionary multiobjective optimization algorithms in which the unit of selection is a hyperbox in objective space. In this technique, instead of assigning a selective fitness to an individual, selective fitness is assigned to the hyperboxes in objective space which are currently occupied by at least one individual in the current approximation to the Pareto frontier. A hyperbox is thereby selected, and the resulting selected individual is randomly chosen from this hyperbox. This method of selection is shown to be more sensitive to ensuring a good spread of development along the Pareto frontier than individual-based selection. The method is implemented in a modern multiobjective evolutionary algorithm, and performance is tested by using Deb's test suite of `T' functions with varying properties. The new selection technique is found to give significantly superior results to the other methods compared, namely PAES, PESA, and SPEA; each is a modern multi-objective optimization algorithm previously found to outperform earlier approaches on various problems.

The automaticity of complex motor skill learning as a function of attentional focus

Abstract: The present experiment was designed to test the predictions of the constrained-action hypothesis. This hypothesis proposes that when performers utilize an internal focus of attention (focus on their movements) they may actually constrain or interfere with automatic control processes that would normally regulate the movement, whereas an external focus of attention (focus on the movement effect) allows the motor system to more naturally self-organize. To test this hypothesis, a dynamic balance task (stabilometer) was used with participants instructed to adopt either an internal or external focus of attention. Consistent with earlier experiments, the external focus group produced generally smaller balance errors than did the internal focus group and responded at a higher frequency indicating higher confluence between voluntary and reflexive mechanisms. In addition, probe reaction times (RTs) were taken as a measure of the attention demands required under the two attentional focus conditions. Consistent with the hypothesis, the external focus participants demonstrated lower probe RTs than did the internal focus participants, indicating a higher degree of automaticity and less conscious interference in the control processes associated with the balance task.

The Diurnal Cycle in the Tropics

Abstract: A global archive of high-resolution (3-hourly, 0.58 latitude‐longitude grid) window (11‐12 mm) brightness temperature (Tb) data from multiple satellites is being developed by the European Union Cloud Archive User Service (CLAUS) project. It has been used to construct a climatology of the diurnal cycle in convection, cloudiness, and surface temperature for all regions of the Tropics. An example of the application of the climatology to the evaluation of the climate version of the U.K. Met. Office Unified Model (UM), version HadAM3, is presented. The characteristics of the diurnal cycle described by the CLAUS data agree with previous observational studies, demonstrating the universality of the characteristics of the diurnal cycle for land versus ocean, clear sky versus convective regimes. It is shown that oceanic deep convection tends to reach its maximum in the early morning. Continental convection generally peaks in the evening, although there are interesting regional variations, indicative of the effects of complex land‐sea and mountain‐valley breezes, as well as the life cycle of mesoscale convective systems. A striking result from the analysis of the CLAUS data has been the extent to which the strong diurnal signal over land is spread out over the adjacent oceans, probably through gravity waves of varying depths. These coherent signals can be seen for several hundred kilometers and in some instances, such as over the Bay of Bengal, can lead to substantial diurnal variations in convection and precipitation. The example of the use of the CLAUS data in the evaluation of the Met. Office UM has demonstrated that the model has considerable difficulty in capturing the observed phase of the diurnal cycle in convection, which suggests some fundamental difficulties in the model’s physical parameterizations. Analysis of the diurnal cycle represents a powerful tool for identifying and correcting model deficiencies.

Dispersal in freshwater invertebrates

Abstract: ▪ Abstract Movement between discrete habitat patches can present significant challenges to organisms. Freshwater invertebrates achieve dispersal using a variety of mechanisms that can be broadly categorized as active or passive, and which have important consequences for processes of colonization, gene flow, and evolutionary divergence. Apart from flight in adult freshwater insects, active dispersal appears relatively uncommon. Passive dispersal may occur through transport by animal vectors or wind, often involving a specific desiccation-resistant stage in the life cycle. Dispersal in freshwater taxa is difficult to study directly, and rare but biologically significant dispersal events may remain undetected. Increased use of molecular markers has provided considerable insight into the frequency of dispersal in freshwater invertebrates, particularly for groups such as crustaceans and bryozoans that disperse passively through the transport of desiccation-resistant propagules. The establishment of propagule b...

A microplate fluorimetric assay for the study of enzyme diversity in soils

Abstract: A fluorimetric microplate enzyme assay has been developed to study enzyme diversity in soil as an approach to understanding functional diversity. The microplate assay allows a large number of soil samples and/or enzymes to be analysed in a short time. The substrates used are conjugates of the highly fluorescent compounds 4-methylumbelliferone (MUB) and 7-amino-4-methyl coumarin (AMC). The main advantage of using fluorimetrically-labelled substrates is that product formation can be measured directly in the microplate without previous extraction and purification of the product. A detailed protocol for this new technology is presented and some potential applications are outlined. A comparative study was carried out between the new microplate fluorimetric assay and a standard colorimetric enzyme assay based on p-nitrophenyl substrates. The kinetics of β-glucosidase and phosphatase activities were investigated in soils with different fertilizer backgrounds. Both methods generated similar values for Vmax (maximum rate of activity) whereas the affinity of β-glucosidase and phosphatase for their respective substrates (as indicated by Km values, Michaelis–Menten constant) was up to two orders of magnitude greater for the 4-methylumbelliferyl substrates compared to the p-nitrophenyl substrates.

Predicting the Mesophases of Copolymer-Nanoparticle Composites

Abstract: The interactions between mesophase-forming copolymers and nanoscopic particles can lead to highly organized hybrid materials. The morphology of such composites depends not only on the characteristics of the copolymers, but also on the features of the nanoparticles. To explore this vast parameter space and predict the mesophases of the hybrids, we have developed a mean field theory for mixtures of soft, flexible chains and hard spheres. Applied to diblock-nanoparticle mixtures, the theory predicts ordered phases where particles and diblocks self-assemble into spatially periodic structures. The method can be applied to other copolymer-particle mixtures and can be used to design novel composite architectures.

Subtropical Anticyclones and Summer Monsoons

Abstract: The summer subtropical circulation in the lower troposphere is characterized by continental monsoon rains and anticyclones over the oceans. In winter, the subtropical circulation is more strongly dominated by the zonally averaged flow and its interactions with orography. Here, the mechanics of the summer and winter lowertropospheric subtropical circulation are explored through the use of a primitive equation model and comparison with observations. By prescribing in the model the heatings associated with several of the world’s monsoons, it is confirmed that the equatorward portion of each subtropical anticyclone may be viewed as the Kelvin wave response to the monsoon heating over the continent to the west. A poleward-flowing low-level jet into a monsoon (such as the Great Plains jet) is required for Sverdrup vorticity balance. This jet effectively closes off the subtropical anticyclone to the east and also transports moisture into the monsoon region. The low-level jet into North America induced by its monsoon heating is augmented by a remote response to the Asian monsoon heating. The Rossby wave response to the west of subtropical monsoon heating, interacting with the midlatitude westerlies, produces a region of adiabatic descent. It is demonstrated here that a local ‘‘diabatic enhancement’’ can lead to a strengthening of the descent. Longitudinal mountain chains act to block the westerly flow and also tend to produce descent in this region. Below the descent, Sverdrup vorticity balance implies equatorward flow that closes off the subtropical anticyclone to the west and induces cool upwelling in the ocean through Ekman transport. Feedbacks, involving, for example, sea surface temperatures, may further enhance the descent in these regions. The conclusion is that the Mediterranean-type climates of regions such as California and Chile may be induced remotely by the monsoon to the east. Hence it can be argued that the subtropical circulation in summer comprises a set of weakly interacting monsoon systems, each involving monsoon rains, a low-level poleward jet, a subtropical anticyclone to the east, and descent and equatorward flow to the west. In winter, it is demonstrated how the nonlinear interaction between the strong zonal-mean circulation, associated with the winter ‘‘Hadley cell,’’ and the mountains can define many of the large-scale features of the subtropical circulation. The blocking effect of the longitudinal mountain chains is shown to be very important. Subsequent diabatic effects, such as a local diabatic enhancement, would appear to be essential for producing the observed amplitude of these features.

A comparative in vitro evaluation of the fermentation properties of prebiotic oligosaccharides

Abstract: Aims: Comparison of in vitro fermentation properties of commercial prebiotic oligosaccharides. Methods and Results: Populations of predominant gut bacterial groups were monitored over 24 h of batch culture through fluorescent in-situ hybridization. Short-chain fatty acid and gas production were also measured. All prebiotics increased the numbers of bifidobacteria and most decreased clostridia. Xylo-oligosaccharides and lactulose produced the highest increases in numbers of bifidobacteria whilst fructo-oligosaccharides produced the highest populations of lactobacilli. Galacto-oligosaccharides (GOS) resulted in the largest decreases in numbers of clostridia. Short-chain fatty acid generation was highest on lactulose and GOS. Gas production was lowest on isomalto-oligosaccharides and highest on inulin. Conclusions: The oligosaccharides differed in their fermentation characteristics. Isomalto-oligosaccharides and GOS were effective at increasing numbers of bifidobacteria and lactate whilst generating the least gas. Significance and Impact of the Study: The study provides comparative data on the properties of commercial prebiotics, allowing targeting of dietary intervention for particular applications and blending of oligosaccharides to enhance overall functionality.

Arctic Oscillation or North Atlantic Oscillation

Abstract: The definition and interpretation of the Arctic oscillation (AO) are examined and compared with those of the North Atlantic oscillation (NAO). It is shown that the NAO reflects the correlations between the surface pressure variability at its centers of action, whereas this is not the case for the AO. The NAO pattern can be identified in a physically consistent way in principal component analysis applied to various fields in the Euro-Atlantic region. A similar identification is found in the Pacific region for the Pacific–North American (PNA) pattern, but no such identification is found here for the AO. The AO does reflect the tendency for the zonal winds at 35° and 55°N to anticorrelate in both the Atlantic and Pacific regions associated with the NAO and PNA. Because climatological features in the two ocean basins are at different latitudes, the zonally symmetric nature of the AO does not mean that it represents a simple modulation of the circumpolar flow. An increase in the AO or NAO implies stro...

North atlantic oscillation - concepts and studies

Abstract: This paper aims to provide a comprehensive review of previous studies and concepts concerning the North Atlantic Oscillation. The North Atlantic Oscillation (NAO) and its recent homologue, the Arctic Oscillation/Northern Hemisphere annular mode (AO/NAM), are the most prominent modes of variability in the Northern Hemisphere winter climate. The NAO teleconnection is characterised by a meridional displacement of atmospheric mass over the North Atlantic area. Its state is usually expressed by the standardised air pressure difference between the Azores High and the Iceland Low. This NAO index is a measure of the strength of the westerly flow (positive with strong westerlies, and vice versa). Together with the El Nino/Southern Oscillation (ENSO) phenomenon, the NAO is a major source of seasonal to interdecadal variability in the global atmosphere. On interannual and shorter time scales, the NAO dynamics can be explained as a purely internal mode of variability of the atmospheric circulation. Interdecadal variability may be influenced, however, by ocean and sea-ice processes.

Clustered Mutations in HIV-1 Gag Are Consistently Required for Escape from Hla-B27–Restricted Cytotoxic T Lymphocyte Responses

Abstract: The immune response to HIV-1 in patients who carry human histocompatibility leukocyte antigen (HLA)-B27 is characterized by an immunodominant response to an epitope in p24 gag (amino acids 263–272, KRWIILGLNK). Substitution of lysine (K) or glycine (G) for arginine (R) at HIV-1 gag residue 264 (R264K and R264G) results in epitopes that bind to HLA-B27 poorly. We have detected a R264K mutation in four patients carrying HLA-B27. In three of these patients the mutation occurred late, coinciding with disease progression. In another it occurred within 1 yr of infection and was associated with a virus of syncytium-inducing phenotype. In each case, R264K was tightly associated with a leucine to methionine change at residue 268. After the loss of the cytotoxic T lymphocyte (CTL) response to this epitope and in the presence of high viral load, reversion to wild-type sequence was observed. In a fifth patient, a R264G mutation was detected when HIV-1 disease progressed. Its occurrence was associated with a glutamic acid to aspartic acid mutation at residue 260. Phylogenetic analyses indicated that these substitutions emerged under natural selection rather than by genetic drift or linkage. Outgrowth of CTL escape viruses required high viral loads and additional, possibly compensatory, mutations in the gag protein.

Major fungal lineages are derived from lichen symbiotic ancestors

Abstract: About one-fifth of all known extant fungal species form obligate symbiotic associations with green algae, cyanobacteria or with both photobionts. These symbioses, known as lichens, are one way for fungi to meet their requirement for carbohydrates. Lichens are widely believed to have arisen independently on several occasions, accounting for the high diversity and mixed occurrence of lichenized and non-lichenized (42 and 58%, respectively) fungal species within the Ascomycota. Depending on the taxonomic classification chosen, 15-18 orders of the Ascomycota include lichen-forming taxa, and 8-11 of these orders (representing about 60% of the Ascomycota species) contain both lichenized and non-lichenized species. Here we report a phylogenetic comparative analysis of the Ascomycota, a phylum that includes greater than 98% of known lichenized fungal species. Using a Bayesian phylogenetic tree sampling methodology combined with a statistical model of trait evolution, we take into account uncertainty about the phylogenetic tree and ancestral state reconstructions. Our results show that lichens evolved earlier than believed, and that gains of lichenization have been infrequent during Ascomycota evolution, but have been followed by multiple independent losses of the lichen symbiosis. As a consequence, major Ascomycota lineages of exclusively non-lichen-forming species are derived from lichen-forming ancestors. These species include taxa with important benefits and detriments to humans, such as Penicillium and Aspergillus.

Active / break cycles: diagnosis of the intraseasonal variability of the Asian Summer Monsoon

Abstract: In this study, various diagnostics have been applied to daily observed outgoing longwave radiation (OLR) and ECMWF ReAnalysis (ERA) products to provide a comprehensive description of the active/break cycles associated with the Asian Summer Monsoon and to address the differing behaviour of the two dominant time scales of intraseasonal variability, 10–20 days and 30–60 days. Composite analysis of OLR based on filtered daily All-India rainfall (AIR) for the 40 day (30–60 days) intraseasonal mode indicates that during active phases, convection is significantly enhanced over the Indian continent, extending over the Bay of Bengal, Maritime continent and equatorial west Pacific, while convection is suppressed over the equatorial Indian Ocean and northwest tropical Pacific, resulting in a ‘quadrapole’ structure over the Asian monsoon domain. In response to this heating pattern, the large-scale Hadley (lateral) and the two east-west (transverse) tropical circulations are enhanced. There is also a significant impact on the extra-tropical circulation through excitation and propagation of Rossby waves. In contrast, the 15-day mode is more regional to the monsoon domain and has a prominent east-west orientation in convection. Only the local Hadley circulation over the monsoon region is modulated by this mode. The evolution of these two modes as revealed by POP (principal oscillation pattern) analysis, shows that the 40-day mode originates over the equatorial Indian Ocean. Once formed it has poleward propagation on either side of the equator, and eastward propagation into the equatorial west Pacific. From the equatorial west Pacific, northward propagation over the west Pacific and westward propagation into the Indian longitudes are prominent. The propagative features are complex and interactive and are responsible for the ‘quadrapole’ structure in convection seen from the composites. The interannual variability, assessed from the POP coefficient time series, indicates that the 40-day mode is strong during the onset phase of the monsoon in all the years but systematic propagation over the entire season depends crucially on the activity of the oceanic TCZ (tropical convergence zone). The POP analysis of the 15-day mode indicates that this event originates over the␣equatorial west Pacific, associated with westward propagating Rossby waves, amplifies over the northwest tropical Pacific and modulates both the continental and oceanic TCZs over Indian longitudes simultaneously. This mode is pronounced during the established phase of the monsoon. Due to the complexity in the propagational features of both the intraseasonal modes, it is concluded that understanding the subseasonal variability of one regional component of the Asian Summer Monsoon (ASM), requires understanding the entire ASM system.

Agricultural Productivity Growth and Poverty Alleviation

Abstract: How important is agricultural growth to poverty reduction? This article first sets out the theoretical reasons for expecting agricultural growth to reduce poverty. Several plausible and strong arguments apply - including the creation of jobs on the land, linkages from farming to the rest of the rural economy, and a decline in the real cost of food for the whole economy - but the degree of impact is in all cases qualified by particular circumstances. Hence, the article deploys a cross-country estimation of the links between agricultural yield per unit area and measures of poverty. This produces strong confirmation of the hypothesised linkages. It is unlikely that there are many other development interventions capable of reducing the numbers in poverty so effectively.

Creative evolutionary systems

Abstract: The use of evolution for creative problem solving is one of the most exciting and potentially significant areas in computer science today. Evolutionary computation is a way of solving problems, or generating designs, using mechanisms derived from natural evolution. This book concentrates on applying important ideas in evolutionary computation to creative areas, such as art, music, architecture, and desgn. It shows how human interaction, new representations, and approaches such as open-ended evolution can extend the capabiities of evolutionary computation from optimization of existing solutions to innovation and the generation of entirely new and original solutions. This book takes a fresh look at creativity, exploring what it is and how the actions of evolution can resemble it. Examples of novel evolved solutions are presented in a variety of creative disciplines. The editors have compiled contributions by leading researchers in each discipline.

Reducing Local Optima in Single-Objective Problems by Multi-objectivization

Abstract: One common characterization of how simple hill-climbing optimization methods can fail is that they become trapped in local optima - a state where no small modification of the current best solution will produce a solution that is better. This measure of 'better' depends on the performance of the solution with respect to the single objective being optimized. In contrast, multi-objective optimization (MOO) involves the simultaneous optimization of a number of objectives. Accordingly, the multi-objective notion of 'better' permits consideration of solutions that may be superior in one objective but not in another. Intuitively, we may say that this gives a hill-climber in multi-objective space more freedom to explore and less likelihood of becoming trapped. In this paper, we investigate this intuition by comparing the performance of simple hill-climber-style algorithms on single-objective problems and multi-objective versions of those same problems. Using an abstract building-block problem we illustrate how 'multi-objectivizing' a single-objective optimization (SOO) problem can remove local optima. Then we investigate small instances of the travelling salesman problem where additional objectives are defined using arbitrary sub-tours. Results indicate that multi-objectivization can reduce local optima and facilitate improved optimization in some cases. These results enlighten our intuitions about the nature of search in multi-objective optimization and sources of difficulty in single-objective optimization.

Hedge Fund Performance 1990-2000: Do the Money Machines Really Add Value?

Abstract: In this paper we investigate the claim that hedge funds offer investors a superior risk-return trade-off. We do so using a continuous time version of Dybvig's (1988a, 1988b) payoff distribution pricing model. The evaluation model, which does not require any assumptions with regard to the return distribution of the funds in question, is applied to the monthly returns of 77 hedge funds and 13 hedge fund indices over the period May 1990-April 2000. The results show that as a stand-alone investment hedge funds do not offer a superior risk-return profile. We find 12 indices and 72 individual funds to be inefficient, with the average efficiency loss amounting to 2.76% per annum for indices and 6.42% for individual funds. Part of the inefficiency cost of individual funds can be diversified away. Funds of funds, however, are not the preferred vehicle for this as their performance appears to suffer badly from their double fee structure. Looking at hedge funds in a portfolio context results in a marked improvement in the evaluation outcomes. Seven of the 12 hedge fund indices and 58 of the 72 individual funds classified as inefficient on a stand-alone basis are capable of producing an efficient payoff profile when mixed with the S&P 500. The best results are obtained when 10-20% of the portfolio value is invested in hedge funds

African Easterly Wave Variability and Its Relationship to Atlantic Tropical Cyclone Activity

Abstract: Automatic tracking of vorticity centers in European Centre for Medium-Range Weather Forecasts analyses has been used to develop a 20-yr climatology of African easterly wave activity. The tracking statistics at 600 and 850 mb confirm the complicated easterly wave structures present over the African continent. The rainy zone equatorward of 15°N is dominated by 600-mb activity, and the much drier Saharan region poleward of 15°N is more dominated by 850-mb activity. Over the Atlantic Ocean there is just one storm track with the 600- and 850-mb wave activity collocated. Based on growth/decay and genesis statistics, it appears that the 850-mb waves poleward of 15°N over land generally do not get involved with the equatorward storm track over the ocean. Instead, there appears to be significant development of 850-mb activity at the West African coast in the rainy zone around (10°N, 10°W), which, it is proposed, is associated with latent heat release. Based on the tracking statistics, it has been shown th...

The buffer effect and large-scale population regulation in migratory birds

Abstract: Buffer effects occur when sites vary in quality and fluctuations in population size are mirrored by large changes in animal numbers in poor-quality sites but only small changes in good-quality sites. Hence, the poor sites ‘buffer’ the good sites1,2, a mechanism that can potentially drive population regulation if there are demographic costs of inhabiting poor sites. Here we show that for a migratory bird this process can apply on a country-wide scale with consequences for both survival and timing of arrival on the breeding grounds (an indicator of reproductive success3,4). The Icelandic population of the black-tailed godwit, Limosa limosa islandica, wintering in Britain has increased fourfold since the 1970s (ref. 5) but rates of change within individual estuaries have varied from zero to sixfold increases. In accordance with the buffer effect, rates of increase are greater on estuaries with low initial numbers, and godwits on these sites have lower prey-intake rates, lower survival rates and arrive later in Iceland than godwits on sites with stable populations. The buffer effect can therefore be a major process influencing large-scale population regulation of migratory species.

The Representation of Snow in Land Surface Schemes: Results from PILPS 2(d)

Abstract: Twenty-one land surface schemes (LSSs) performed simulations forced by 18 yr of observed meteorological data from a grassland catchment at Valdai, Russia, as part of the Project for the Intercomparison of Land-Surface Parameterization Schemes (PILPS) Phase 2(d). In this paper the authors examine the simulation of snow. In comparison with observations, the models are able to capture the broad features of the snow regime on both an intra- and interannual basis. However, weaknesses in the simulations exist, and early season ablation events are a significant source of model scatter. Over the 18-yr simulation, systematic differences between the models’ snow simulations are evident and reveal specific aspects of snow model parameterization and design as being responsible. Vapor exchange at the snow surface varies widely among the models, ranging from a large net loss to a small net source for the snow season. Snow albedo, fractional snow cover, and their interplay have a large effect on energy available for ablation, with differences among models most evident at low snow depths. The incorporation of the snowpack within an LSS structure affects the method by which snow accesses, as well as utilizes, available energy for ablation. The sensitivity of some models to longwave radiation, the dominant winter radiative flux, is partly due to a stability-induced feedback and the differing abilities of models to exchange turbulent energy with the atmosphere. Results presented in this paper suggest where weaknesses in macroscale snow modeling lie and where both theoretical and observational work should be focused to address these weaknesses.

Potential local regulatory functions of inhibins, activins and follistatin in the ovary.

Abstract: The changing pattern of granulosa cell expression of inhibin/activin subunits and follistatin during follicle development and their differential regulation by extrinsic and intraovarian factors supports evidence from functional studies, mostly in vitro, that these proteins have important roles in folliculogenesis, oocyte maturation and corpus luteum function. Gonadal inhibins function as negative feedback hormones to regulate the synthesis and secretion of pituitary FSH, a key determinant of follicle development, but there is little supportive evidence for a peripheral endocrine role for ovary-derived activins or follistatin in this regard. However, activins and follistatin are expressed in numerous other tissues, including anterior pituitary, and they are firmly implicated as local intrapituitary regulators of FSH secretion. Intraovarian actions of granulosa cell-derived activins include the promotion of granulosa cell proliferation and upregulation of FSH receptors, P450arom, oestrogen synthesis, granulosa cell LH receptors and enhancement of oocyte maturation. Through its activin-binding role, follistatin can reverse each of these activin-induced responses. In addition to their endocrine feedback role, granulosa-derived inhibins can sensitize theca cells to LH, thereby enhancing the production of androgens, an essential requirement for follicular oestrogen synthesis. Activins can oppose this effect and suppress thecal androgen production. Granulosa cells overproduce inhibin a subunit precursor relative to betaA/betaB subunit precursors and evidence indicates that different parts of the inhibin a subunit precursor have intrinsic biological activities distinct from inhibin alphabetaA/B dimer, and serve as additional local modulators of follicle and corpus luteum function.

The tempo of Holocene climatic change in the eastern Mediterranean region: new high-resolution crater-lake sediment data from central Turkey:

Abstract: This study presents results from a multi-proxy analysis of cores taken in a crater-lake sequence from Eski Acigol in central Turkey which cover the period from pre-c. 16000 cal. yr BP to the present. The sediments comprise an upper unit of generally non-laminated, banded to massive silts and peats of mid- to late-Holocene age, overlying a laminated unit of late-Pleistocene to early/mid-Holocene age. The laminae, comprising mainly aragonite, amorphous silica (diatom frustules) and organic matter were formed in a relatively deep, dilute, meromictic lake. Pollen data indicate an abrupt replacement of Artemisia-chenopod steppe by grass-oak-terebinth parkland during the period of laminae deposition, marking the start of the Holocene. A gradual increase in tree pollen during the early Holocene came to an end c. 6500 cal. yr BP (U-series and adjusted C-14 timescale), when mesic deciduous taxa declined at the same time as lake levels fell. Human impact on regional vegetation is inferred from a sharp decline in oak around 4500-4000 cal. yr BP. Diatom, isotopic and mineralogical data indicate that during the second half of the Holocene the lake became relatively shallow and oscillated between fresh and brackish/evaporated water conditions. The contrast between wetter early- and drier late-Holocene climatic conditions is matched by other eastern and central Mediterranean proxy climate data. While the Eski Acigol sequence resembles Holocene hydroclimatic changes in the Saharo-Arabian zone and was also apparently linked to orbital forcing, it is unlikely to have had the same direct cause, i.e., an expansion and subsequent retreat of monsoon rainfall.

The prebiotic effects of biscuits containing partially hydrolysed guar gum and fructo-oligosaccharides – a human volunteer study

Abstract: Prebiotics are non-digestible food ingredients that target selected groups of the human colonic microflora, thus having the ability to alter the composition towards a more 'beneficial' community, i.e. selectively increasing populations of bifidobacteria and/or lactobacilli. In the present study the prebiotic potential of partially hydrolysed guar gum (PHGG) and fructo-oligosaccharides (FOS) in a biscuit was assessed in human volunteers. Fluorescent in situ hybridization using oligonucleotide probes targeting Bacteroides spp., Bifidobacterium spp., Clostridium spp. and Lactobacillus-Enterococcus spp. were used for the bacteriology and total bacteria were enumerated using the fluorescent stain 4',6-diamidino-2-phenylindole. Thirty-one volunteers consumed daily either three experimental biscuits (providing a total (g/d) of 6.6 FOS and 3.4 PHGG) or three placebo biscuits for two 21-d crossover periods. Bifidobacteria significantly increased in number on ingestion of the experimental biscuits compared with pre-treatment and placebo population levels. Bifidobacterial numbers returned to pretreatment levels within 7 d of the cessation of intake of experimental biscuits. A correlation was observed between the initial faecal bifidobacterial numbers and the magnitude of bifidogenesis, with volunteers who possessed low initial population levels of bifidobacteria experiencing the greatest increase in bifidogenesis. No changes were observed in the other bacterial groups monitored during the trial. Thus, the prebiotic nature of FOS and PHGG was maintained in a final food product as evidenced from the selective increase in bifidobacterial numbers.

Antioxidant Activity of Hydroxytyrosol Acetate Compared with That of Other Olive Oil Polyphenols

Abstract: Hydroxytyrosol acetate was synthesized, and the antioxidant activity of this olive oil component was assessed in comparison with that of other olive oil components, namely hydroxytyrosol, oleuropein, 3,4-DHPEA-EA, and alpha-tocopherol in bulk oil and oil-in-water emulsions. The activity of the compounds was also assessed by scavenging of 1,1-diphenyl-2-picrylhydrazyl (DPPH) radicals. Hydroxytyrosol acetate had a weaker DPPH radical scavenging activity than hydroxytyrosol, oleuropein, or 3,4-DHPEA-EA but it had a radical scavenging activity similar to that of alpha-tocopherol. In oil, the antioxidant activity of hydroxytyrosol acetate was much higher than that of alpha-tocopherol or oleuropein, but in an emulsion 3,4-DHPEA-EA and alpha-tocopherol were more effective as antioxidants than hydroxytyrosol acetate. The antioxidant activity of hydroxytyrosol acetate was rather similar to that of hydroxytyrosol in oil and emulsions despite the difference in DPPH radical scavenging activity.