Institution
University of Rijeka
Education•Rijeka, Croatia•
About: University of Rijeka is a education organization based out in Rijeka, Croatia. It is known for research contribution in the topics: Population & Tourism. The organization has 3471 authors who have published 7993 publications receiving 110386 citations. The organization is also known as: Rijeka University & Sveučilište u Rijeci.
Topics: Population, Tourism, European union, Immune system, Cytotoxic T cell
Papers published on a yearly basis
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TL;DR: Experimental results suggest potential clinical application of computer-aided decision making, both for detecting milder injuries and detecting complete ruptures in a human knee.
53 citations
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TL;DR: The plasma levels of pro-inflammatory cytokines during the course of primary Listeria monocytogenes and Campylobacter jejuni infection were quantified to assess the relationship between endogenous cytokines and the bacterial number in the liver of infected animals.
Abstract: Background: Exposure to microorganisms elicts the production of cytokines. These soluble factors enhance several innate immune functions and regulate the ensuing specific immune response aimed at limiting the spread of infection. Aim: This study was undertaken to quantify the plasma levels of pro-inflammatory cytokines during the course of primary Listeria monocytogenes and Campylobacter jejuni infection. Using an in vivo infection the relationship between endogenous cytokines and the bacterial number in the liver of infected animals was examined. Methods: C57BL/6 mice were infected by the intraperitoneal route. At different time points we determined the number of colony-forming units of bacteria in the liver of infected animals and paralled these with the plasma levels of interferon-gamma (IFN-g ), tumor necrosis factor-alpha (TNF-a ) and interleukin6 (IL-6) measured by enzyme immunoassays. Results: L. monocytogenes infection lasted 10‐11 days. IFN-g production occurred in the early phase but was more pronounced after day 4, following the appearance of specific immunity. The duration of experimental campylobacteriosis was 15 days. Early IFN-g production was not significant but a progressive rise of this cytokine in plasma was seen during the second week post infection. Mice produced measurable amounts of plasma TNF-a immediately after being given viable L. monocytogenes, peaking on day 2‐3 when the greatest number of bacteria was present in the examined organs. During C. jejuni infection plasma TNF-a was produced in a similar manner, but the highest concentrations were found a few days later than in listeriosis, in correlation with the different course of campylobacteriosis. The quantity of IL-6 increased and decreased in concordance with clearance of L. monocytogenes and the clinical status of the animals. C. jejuni did not promote the induction of this cytokine. This is to some extent an unusual finding. With respect to the role of IL-6 in Th2 responses and antibody production, the appearance of this cytokine in campylobacteriosis was more expected. Discussion: During systemic bacterial infection, a network of pro-inflammatory cytokines is activated and blood levels of these cytokines are elevated, albeit inconsistently, with large individual variations and depending on microbial characteristics and structure.
53 citations
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TL;DR: A new tumor immune evasion mechanism that is mediated through the interaction between PS and CD300a is demonstrated and it is suggested that CD300c, similarly toCD300a, also interacts with PS.
Abstract: The activity of NK cells is controlled by inhibitory and activating receptors. The inhibitory receptors interact mostly with MHC class I proteins, however, inhibitory receptors such as CD300a, which bind to non-MHC class I ligands, also exist. Recently, it was discovered that phosphatidylserine (PS) is a ligand for CD300a and that the interaction between PS expressed on apoptotic cells and CD300a inhibits the uptake of apoptotic cells by phagocytic cells. Whether PS can inhibit NK-cell activity through CD300a is unknown. Here, we have generated specific antibodies directed against CD300a and we used these mAbs to demonstrate that various NK-cell clones express different levels of CD300a. We further demonstrated that both CD300a and its highly homologous molecule CD300c bind to the tumor cells equally well and that they recognize PS and additional unknown ligand(s) expressed by tumor cells. Finally, we showed that blocking the PS-CD300a interaction resulted in increased NK-cell killing of tumor cells. Collectively, we demonstrate a new tumor immune evasion mechanism that is mediated through the interaction between PS and CD300a and we suggest that CD300c, similarly to CD300a, also interacts with PS.
53 citations
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TL;DR: Use of a spread-deficient murine CMV (MCMV) as a novel approach for betaherpesvirus vaccination is described and the linkage between cell tropism and immunogenicity is allowed.
Abstract: Human cytomegalovirus (HCMV) is a human pathogen that causes severe disease primarily in the immunocompromised or immunologically immature individual. To date, no vaccine is available. We describe use of a spread-deficient murine CMV (MCMV) as a novel approach for betaherpesvirus vaccination. To generate a spread-deficient MCMV, the conserved, essential gene M94 was deleted. Immunization with MCMV-DeltaM94 is apathogenic and protective against wild-type challenge even in highly susceptible IFNalphabetaR(-/-) mice. MCMV-DeltaM94 was able to induce a robust CD4(+) and CD8(+) T-cell response as well as a neutralizing antibody response comparable to that induced by wild-type infection. Endothelial cells were identified as activators of CD8(+) T cells in vivo. Thus, the vaccination with a spread-deficient betaherpesvirus is a safe and protective strategy and allows the linkage between cell tropism and immunogenicity. Furthermore, genomes of MCMV-DeltaM94 were present in lungs 12 months after infection, revealing first-target cells as sites of genome maintenance.
53 citations
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TL;DR: In this article, the authors consider the non-stationary 3D flow of a compressible viscous heat-conducting micropolar fluid in the domain that is the subset of R 3 bounded with two concentric spheres that present solid thermo-insulated walls.
52 citations
Authors
Showing all 3537 results
Name | H-index | Papers | Citations |
---|---|---|---|
Igor Rudan | 142 | 658 | 103659 |
Nikola Godinovic | 138 | 1469 | 100018 |
Ivica Puljak | 134 | 1436 | 97548 |
Damir Lelas | 133 | 1354 | 93354 |
D. Mekterovic | 110 | 449 | 46779 |
Ulrich H. Koszinowski | 96 | 281 | 27709 |
Michele Doro | 79 | 437 | 20090 |
Robert Zivadinov | 73 | 522 | 18636 |
D. Dominis Prester | 70 | 363 | 16701 |
Daniel Ferenc | 70 | 225 | 16145 |
Vladimir Parpura | 64 | 226 | 18050 |
Stipan Jonjić | 62 | 227 | 19363 |
Dario Hrupec | 60 | 288 | 13345 |
Alessandro Laviano | 59 | 298 | 14609 |
Tomislav Terzić | 58 | 271 | 10699 |