University of Salford

About: University of Salford is a education organization based out in Salford, Manchester, United Kingdom. It is known for research contribution in the topics: Population & Thin film. The organization has 13049 authors who have published 22957 publications receiving 537330 citations. The organization is also known as: University of Salford Manchester & The University of Salford Manchester.

Novel method for the isolation and characterisation of the putative prostatic stem cell.

Abstract: Background Prostate stem cells, responsible for the development, maturation, and function of the prostate, have been implicated in the aetiology of both benign prostate hyperplasia (BPH) and prostate cancer (CaP). However, research has been hampered by the lack of a definitive stem cell marker. We have adapted the protocol for differential Hoechst 33342 uptake by hemopoietic stem cells to enable isolation of putative stem cells from the prostate. Methods Prostate epithelial cells isolated from prostate tissue obtained from patients with BPH after transurethral resection of the prostate were stained with Hoechst 33342. The Hoechst 33342 Red/Blue flow cytometry profile was then determined. Hoechst 33342 and Pyronin Y staining was used to determined the cell cycle status. Results A verapamil-sensitive side population (SP) can be isolated from primary prostate tissue accounting for 1.38% ± 0.07% of prostate epithelial cells. Cell cycle analysis of this SP population revealed that the majority of SP cells are in either G0 (12.38 ± 0.31%) or G1 (63.19 ± 2.13%). Conclusions The Hoechst 33342 dye efflux protocol can be adapted for the isolation of a SP from primary prostate tissue. Cytometry Part A 54A:89–99, 2003. © 2003 Wiley-Liss, Inc.

Involving Young Service Users as Co-Researchers: Possibilities, Benefits and Costs

Abstract: This article seeks to contribute to the debate concerning the benefits and costs of involving young service users in research. The paper locates involvement within a continuum of consultation, collaboration and user-controlled research. The mandate for children and young people’s involvement is identified. In particular, the paper focuses on the benefits and costs in relation to: research and development, research dissemination and service development, service users and researchers. The paper does not suggest that these benefits and costs can be measured arithmetically but argues that if the costs in terms of resources, training, support, timescale and remuneration are not addressed, the research will be undermined and in danger of becoming tokenistic. The article argues that the involvement of young service users as co-researchers is worthwhile, but that it should not be entered into lightly and that further work needs to be undertaken on which parts of the process young service users can be included in and where their involvement results in change in service delivery or service outcomes.

Braided pneumatic actuator control of a multi-jointed manipulator

Abstract: Problems with control and compliance of pneumatic systems have prevented their widespread use in advanced robotics. However, their compactness, power/weight ration and safety are factors that could potentially be exploited in sophisticated dexterous manipulator designs. This paper reports on the design of a dexterous manipulation with three fingers and an opposal thumb powered by 18 new compact braided pneumatic muscle actuators (PMA). These muscles (which are variants on the McKibben Muscle design) have considerable power output potential, combined with controllable motion and inherent compliance to prevent damage to handled objects. When arranged as antagonistic pairs the joints of the gripper can be driven independently. Using experimental data a system model can be developed and control of these muscles can be explored showing that accurate regulated performance is feasible. >