University of Southern Denmark

About: University of Southern Denmark is a education organization based out in Odense, Syddanmark, Denmark. It is known for research contribution in the topics: Population & Randomized controlled trial. The organization has 11928 authors who have published 37918 publications receiving 1258559 citations. The organization is also known as: SDU.

Rapid induction and reversal of a bacteriostatic condition by controlled expression of toxins and antitoxins

Abstract: RelE and ChpAK (MazF) toxins of Escherichia coli have previously been described as proteins that mediate efficient cell killing. We show here that induction of relE or chpAK transcription does not confer cell killing but, instead, induces a static condition in which the cells are still viable but unable to proliferate. Later induction of transcription of the antitoxin genes relB or chpAI fully reversed the static condition induced by RelE and ChpAK respectively. We also provide a mechanistic explanation for these findings. Thus, induction of relE transcription severely inhibited translation, whereas induction of chpAK transcription inhibited both translation and replication. Hence, most likely, lack of colony formation is due to inhibition of translation in the case of relE and inhibition of translation and/or replication in the case of chpAK. Consistent with this proposal, later induction of transcription of the cognate antitoxin genes simultaneously reversed cell stasis and the inhibitory effects of RelE and ChpAK on macromolecular syntheses. These results preclude that RelE and ChpAK mediate cell killing during the conditions used here. In vivo and in vitro analyses of a mutant RelE protein supported that inhibition of colony formation was due to inhibition of translation.

Pre-mRNA splicing and mRNA export linked by direct interactions between UAP56 and Aly

Abstract: Recent studies indicate that splicing of pre-messenger RNA and export of mRNA are normally coupled in vivo1,2,3,4,5,6. During splicing, the conserved mRNA export factor Aly is recruited to the spliced mRNA–protein complex (mRNP), which targets the mRNA for export. At present, it is not known how Aly is recruited to the spliced mRNP. Here we show that the conserved DEAD-box helicase UAP56, which functions during spliceosome assembly7,8,9,10, interacts directly and highly specifically with Aly. Moreover, UAP56 is present together with Aly in the spliced mRNP. Significantly, excess UAP56 is a potent dominant negative inhibitor of mRNA export. Excess UAP56 also inhibits the recruitment of Aly to the spliced mRNP. Furthermore, a mutation in Aly that blocks its interaction with UAP56 prevents recruitment of Aly to the spliced mRNP. These data suggest that the splicing factor UAP56 functions in coupling the splicing and export machineries by recruiting Aly to the spliced mRNP.

Hypothermia versus Normothermia after Out-of-Hospital Cardiac Arrest.

Abstract: Background Targeted temperature management is recommended for patients after cardiac arrest, but the supporting evidence is of low certainty. Methods In an open-label trial with blinded as...

Reexamination of validity and reliability of the CSA monitor in walking and running.

Abstract: BRAGE, S., N. WEDDERKOPP, P. W. FRANKS, L. B. ANDERSEN, and K. FROBERG. Reexamination of Validity and Reliability of the CSA Monitor in Walking and Running. Med. Sci. Sports Exerc., Vol. 35, No. 8, pp. 1447–1454, 2003.PurposeTo evaluate the reliability and validity of the CSA (model 7164) ac