University of Tabriz

About: University of Tabriz is a education organization based out in Tabriz, Iran. It is known for research contribution in the topics: Population & Nanocomposite. The organization has 12141 authors who have published 20976 publications receiving 313982 citations.

Up regulation of Bax and down regulation of Bcl2 during 3-NC mediated apoptosis in human cancer cells

Abstract: Recently, we have reported the induction of apoptosis by 2-amino-4-(3-nitrophenyl)-3-cyano-7-(dimethylamino)-4H-chromene (3-NC) in HepG2, T47D and HCT116 cells with low nano molar IC50 values. In this study, anti-proliferative effects of modified 4-aryle-4H-chromenes derivatives; 2-amino-4-(3-bromophenyl)-3-cyano-7-(dimethylamino)-4H-chromene (3-BC), 2-amino-4-(3-trifluoromethylphenyl)-3-cyano-7-(dimethylamino)-4H-chromene (3-TFC) and 2-amino-4-(4,5-methylenedioxyphenyl)-3-cyano-7-(dimethylamino)-4H-chromene (4, 5-MC) were investigated in three human cancer cell lines. Compared to 3-NC none of the compounds displayed better anti-proliferative effect, although 3-BC appeared somewhat similar. Therefore 3-NC was selected for further studies. Treatment of HepG2, T47D and HCT116 cells with this compound induced apoptosis as visualized by fluorescence microscopic study of Hoechst 33258 stained cells. Induction of apoptosis was quantified by Annexin V/PI staining using flow cytometry. Western blot analysis also revealed that 3-NC down-regulated the expression of anti-apoptotic protein Bcl2 and up-regulated pro-apoptotic protein Bax, in all of the cell lines. Nonetheless, HepG2 cell line was the most responsive to 3-NC as Bax and Bcl2 showed the most dramatic up and down regulation. Our previous finding that 3-NC down regulates Inhibitor of Apoptosis Proteins (IAPs) and the present observation that Bax is upregulated and Bcl2 is down regulated upon 3-NC treatment, this chromene derivative has the potential to overcome chemotherapy resistance caused by up regulation of these proteins.

Optimization of electrocoagulation process for removal of an azo dye using response surface methodology and investigation on the occurrence of destructive side reactions

Abstract: The optimization and modeling of the electrocoagulation process which conducted by means of iron (EC-Fe) and aluminum (EC-Al) anodes, in the removal of C.I. Reactive Red 43 were performed through the response surface methodology (RSM). Moreover, the occurrence of possible destructive reactions during both EC-Fe and EC-Al processes was investigated using UV–Vis spectrometry, total organic carbon (TOC) and GC–MS analyses. The electrocoagulation experiments were carried out in a monopolar batch reactor using two anodes and two cathodes in parallel connections. Current density, time, pH and chloride concentration were considered as input variables for RSM. The analysis of variance revealed a high coefficient of determination ( R EC-Fe 2 = 0.981 and R EC-Al 2 = 0.934 ) between experimental removal efficiency and predicted one by RSM developed models. The optimum conditions proposed by RSM to reach the maximum RR43 removal through the EC-Fe were different from the ones proposed for the EC-Al. At the optimum conditions, the removal efficiency of dye was more than 99% for both processes, whereas 90.58% and 98.37% of initial TOC concentration decreased during EC-Fe and EC-Al processes, respectively. The analyses results confirmed that beside sweep flocculation mechanism, known as the main removal mechanism, the degradation of dye was occurred during EC-Fe as a minor pathway.