University of Texas at Austin

About: University of Texas at Austin is a education organization based out in Austin, Texas, United States. It is known for research contribution in the topics: Population & Poison control. The organization has 94352 authors who have published 206297 publications receiving 9070052 citations. The organization is also known as: UT-Austin & UT Austin.

Starch gel electrophoresis of enzymes—A compilation of recipes

Abstract: The technique of starch gel electrophoresis of enzymes with specific staining for activity in the gel, the so-called zymogram method developed by Hunter and Markert (1957), has found many research applications. Screening studies, comparing relatively large numbers of enzymes among a variety of tissues and organisms, are finding increasing use in research on population genetics, taxonomy, etc. The methods presently available are scattered through the literature. This compilation is published in response to numerous requests from investigators in many fields. Most of the methods outlined here have been used primarily for tissue extracts from mammalian species. A few have been developed only on plants or lower animals, but this is not to say that they will not work on higher organisms. The buffer systems have been for the most part empirically arrived at. All are probably subject to improvement. The techniques of making the gels and carrying out the electrophoresis are not described here. These have been amply presented in a number of publications. The vertical system is essentially that described by Shaw and Koen (1968b). The horizontal system is essentially as described by Beckman and Johnson (1964). The horizontal method has the advantage of employing simpler and less expensive apparatus, and it can be run at room temperature using ice trays to keep the gels cold. For many enzyme systems, it provides as good results as the vertical. Both Connaught starch (Connaught Laboratories, Toronto) and Electrostarch (Otto Hiller Company, Madison, Wisconsin) will give satisfactory results with either technique. Unfortunately, different batches of starch may produce varying results, both in rate of migration and in resolution; also, some batches contain substances

Hydrogels in regenerative medicine

Abstract: Hydrogels, due to their unique biocompatibility, flexible methods of synthesis, range of constituents, and desirable physical characteristics, have been the material of choice for many applications in regenerative medicine. They can serve as scaffolds that provide structural integrity to tissue constructs, control drug and protein delivery to tissues and cultures, and serve as adhesives or barriers between tissue and material surfaces. In this work, the properties of hydrogels that are important for tissue engineering applications and the inherent material design constraints and challenges are discussed. Recent research involving several different hydrogels polymerized from a variety of synthetic and natural monomers using typical and novel synthetic methods are highlighted. Finally, special attention is given to the microfabrication techniques that are currently resulting in important advances in the field.

The Role of Beliefs in the Practice of Teaching.

Abstract: (1987). The role of beliefs in the practice of teaching. Journal of Curriculum Studies: Vol. 19, No. 4, pp. 317-328.

Moiré bands in twisted double-layer graphene

Abstract: A moire pattern is formed when two copies of a periodic pattern are overlaid with a relative twist. We address the electronic structure of a twisted two-layer graphene system, showing that in its continuum Dirac model the moire pattern periodicity leads to moire Bloch bands. The two layers become more strongly coupled and the Dirac velocity crosses zero several times as the twist angle is reduced. For a discrete set of magic angles the velocity vanishes, the lowest moire band flattens, and the Dirac-point density-of-states and the counterflow conductivity are strongly enhanced.

The prevalence and characteristics of fibromyalgia in the general population

Abstract: Objective. To determine the prevalence and characteristics of fibromyalgia in the general population. Methods. A random sample of 3,006 persons in Wichita, KS, were characterized according to the presence of no pain, non-widespread pain, and widespread pain. A subsample of 391 persons, including 193 with widespread pain, were examined and interviewed in detail. Results. The prevalence of fibromyalgia was 2.0% (95% confidence interval [95% CI] 1.4, 2.7) for both sexes, 3.4% (95% CI 2.3, 4.6) for women, and 0.5% (95% CI 0.0, 1.0) for men. The prevalence of the syndrome increased with age, with highest values attained between 60 and 79 years (>7.0% in women). Demographic, psychological, dolorimetry, and symptom factors were associated with fibromyalgia. Conclusion. Fibromyalgia is common in the population, and occurs often in older persons. Characteristic features of fibromyalgia–pain threshold and symptoms–are similar in community and clinic populations, but overall severity, pain, and functional disability are more severe in the clinic population.