University of Texas Health Science Center at Houston

About: University of Texas Health Science Center at Houston is a education organization based out in Houston, Texas, United States. It is known for research contribution in the topics: Population & Poison control. The organization has 27309 authors who have published 42520 publications receiving 2151596 citations. The organization is also known as: UTHealth & The UT Health Science Center at Houston.

Guidelines for the use and interpretation of assays for monitoring autophagy (4th edition)

Abstract: In 2008, we published the first set of guidelines for standardizing research in autophagy. Since then, this topic has received increasing attention, and many scientists have entered the field. Our knowledge base and relevant new technologies have also been expanding. Thus, it is important to formulate on a regular basis updated guidelines for monitoring autophagy in different organisms. Despite numerous reviews, there continues to be confusion regarding acceptable methods to evaluate autophagy, especially in multicellular eukaryotes. Here, we present a set of guidelines for investigators to select and interpret methods to examine autophagy and related processes, and for reviewers to provide realistic and reasonable critiques of reports that are focused on these processes. These guidelines are not meant to be a dogmatic set of rules, because the appropriateness of any assay largely depends on the question being asked and the system being used. Moreover, no individual assay is perfect for every situation, calling for the use of multiple techniques to properly monitor autophagy in each experimental setting. Finally, several core components of the autophagy machinery have been implicated in distinct autophagic processes (canonical and noncanonical autophagy), implying that genetic approaches to block autophagy should rely on targeting two or more autophagy-related genes that ideally participate in distinct steps of the pathway. Along similar lines, because multiple proteins involved in autophagy also regulate other cellular pathways including apoptosis, not all of them can be used as a specific marker for bona fide autophagic responses. Here, we critically discuss current methods of assessing autophagy and the information they can, or cannot, provide. Our ultimate goal is to encourage intellectual and technical innovation in the field.

The Structure of Ethnic Identity of Young Adolescents from Diverse Ethnocultural Groups

Abstract: The purpose for this study was to examine the structure and construct validity of a measure of ethnic identity among young adolescents from diverse ethnic groups. Students in sixth, seventh, and eighth grades (N = 5,423) from diverse ethnic groups completed the Multigroup Ethnic Identity Measure (MEIM), measures of psychological well-being and a measure of salience of ethnicity. Factor analyses of data for the three largest ethnic groups (European American, African American, Mexican American) yielded a two-factor structure that corresponded to two theoretical approaches to ethnic identity, as hypothesized. Similar patterns in magnitude of loadings were observed across groups, indicating that the MEIM could be used as a global composite index of ethnic identity. Ethnic identity was related positively to measures of psychological well-being such as coping ability, mastery, self-esteem and optimism, and negatively to measures of loneliness and depression. MEIM scores also were moderately strong and positive ...

Update of the Stroke Therapy Academic Industry Roundtable Preclinical Recommendations

Abstract: The initial Stroke Therapy Academic Industry Roundtable (STAIR) recommendations published in 1999 were intended to improve the quality of preclinical studies of purported acute stroke therapies Although recognized as reasonable, they have not been closely followed nor rigorously validated Substantial advances have occurred regarding the appropriate quality and breadth of preclinical testing for candidate acute stroke therapies for better clinical translation The updated STAIR preclinical recommendations reinforce the previous suggestions that reproducibly defining dose response and time windows with both histological and functional outcomes in multiple animal species with appropriate physiological monitoring is appropriate The updated STAIR recommendations include: the fundamentals of good scientific inquiry should be followed by eliminating randomization and assessment bias, a priori defining inclusion/exclusion criteria, performing appropriate power and sample size calculations, and disclosing potential conflicts of interest After initial evaluations in young, healthy male animals, further studies should be performed in females, aged animals, and animals with comorbid conditions such as hypertension, diabetes, and hypercholesterolemia Another consideration is the use of clinically relevant biomarkers in animal studies Although the recommendations cannot be validated until effective therapies based on them emerge from clinical trials, it is hoped that adherence to them might enhance the chances for success

Statistical Methods in Medical Research

Abstract: Since John Snow first conducted a modern epidemiological study in 1854 during a cholera epidemic in London, statistics has been associated with medical research. After Austin Bradford Hill published a series of articles on the use of statistical methodology in medical research in 1937, statistical considerations and computational tools have been paramount in conductingmedical research [1]. For the past century, statistics has played an important role in the advancement of medical research and medical research has stimulated rapid development of statistical methods. For example, the development of modern survival analysis-an important branch of statistics has aimed to solve problems encountered in clinical trials and large-scale epidemiological studies. In this era of evidence-based medicine, the development of novel statistical methods will continue to be crucial in medical research. With the expansion of computer capacity and advancement of computational techniques, it is inevitable that modern statistical methods will likely incorporate, to a greater degree, complex computational procedures. This issue focuses on statistical methods in medical research. Several novel methods aiming on solving different medical research questions are introduced. Some unique approaches of statistical analysis are also present. Hanagal and Sharma contribute two papers. The first one deals with a bivariate survival model. They examine a parameter estimation issue when the samples are taken from a bivariate log-logistic distribution with shared gamma frailty. They propose to use a Bayesian approach along with theMarkov ChainMonte Carlo computational technique for implementation. The computer simulation is conducted for performance evaluation. Two well-known datasets, one about acute leukemia and the other about kidney infection are applied as examples. The second paper contributed by Hanagal and Sharma examines the shared inverse Gaussian frailty model with the bivariate exponential baseline hazard. They first derive the likelihood of the joint survival function. In their Bayesian approach, the parameters of the baseline hazard are assumed to follow a gamma distribution while the coefficients of the regression relationship are assumed to follow an independent normal distribution. The dependence of two components of the survival function is tested. Three information criteria are used for model comparisons. The proposed method is applied to analyze diabetic retinopathy data. The paper by Chang, Lyer, Bullitt and Wang provides a method to find determinants of the brain arterial system. They represent the brain arterial system as a binary tree and apply the mixed logistic regression model to find significant covariates. The authors also demonstrate model selection methods for both fixed and random effects. A case study is presented using the method. This paper provides a rigorous approach for analyzing the binary branching structure data. It is potentially applicable to other tree structure data. Chakraborty proposes two probabilistic models to estimate male-to-female HIV-1 transmission rate in one sexual contact. One model is applicable when the transmitter cell counts are known and the other model is applicable when the receptor cell counts are known. By first uniformizing each transmitter (or receptor) cell count and assuming as a beta distribution, this paper algebraically derives the transition probability by imposing some boundary conditions based on scientific phenomena related to HIV infection. The paper by Yeh, Jiang, Garrard, Lei and Gajewski proposes to use a zero-truncated Poisson model to analyze human cancer tissues transplanted to mice when the positive counts of affected ducts is subject to right censoring. A Bayesian approach choosing a Gamma distribution as the prior is adopted. After implementing through complex computational procedures, this paper obtains the estimates of the coefficients and demonstrates model fitting through