Showing papers by "University of Turin published in 1976"
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TL;DR: Carbon monoxide chemisorption was investigated at 36 °C on four transition aluminas (η, γ, θ, Alon C) by means of microcalorimetry and infrared spectroscopy.
129 citations
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TL;DR: The problem of inducing an algorithm for a partial recursive function from the analysis of its graph or loosely speaking of “learning” it, is discussed.
98 citations
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TL;DR: A new type of hemoglobin F, in which isoleucine in position 75 (E 19) of the γ chain is replaced by a threonine residue, has been found in 29 out of 32 homozygotes for β thalassemia.
Abstract: A new type of hemoglobin F, in which isoleucine in position 75 (E 19) of the γ chain is replaced by a threonine residue, has been found in 29 out of 32 homozygotes for β thalassemia. The amount of this hemoglobin ranges from traces to 40% of the total Hb F. The same γ75 Thr chain is also present in the Hb F of 40% of normal newborns and premature infants examined, of one 14-week-old fetus and in one out of 3 patients with aplastic anemia and raised levels of Hb F. Our results strongly suggest that the synthesis of this new chain is under the control of a γ gene nonallelic with those coding for Aγ and Gγ chains.
83 citations
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TL;DR: Prolonged treatment of rats with a cholesterol-rich diet induced hypercholesterolaemia and increased free cholesterol content of peritoneal macrophages and these cells showed a significant inhibition of phagocytosis.
Abstract: Prolonged treatment of rats with a cholesterol-rich diet induced hypercholesterolaemia and increased free cholesterol content of peritoneal macrophages. A 2-2 times increase in plasma membrane cholesterol was demonstrated in cholesterol-enriched macrophages. These cells showed a significant inhibition of phagocytosis. The inhibition was 37-0% and 81-7% for latex particles or lipid droplets, respectively.
54 citations
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TL;DR: Kinetic data, obtained by the stopped-flow technique, showed that the reactions progress through an SN 1 mechanism according to: FeOH2 + H2cat⇄krkƒFecat+ + H+ +H2O, and kf values are independent of substituent groups present on the ligand, which affect only the reverse reaction rates.
50 citations
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TL;DR: A case of immune-complex glomerulonephritis with membrano-proliferative patterns in a patient with mixed IgG-IgM cryoglobulinemia has been investigated, with particular attention paid to histological, immunofluorescence, histochemical and electron microscopical findings in order to elucidate the nature of the intraluminal thrombi.
Abstract: A case of immune-complex glomerulonephritis with membrano-proliferative patterns in a patient with mixed IgG-IgM cryoglobulinemia has been investigated; particular attention has been payed to histological, immunofluorescence, histochemical and electron microscopical findings in order to elucidate the nature of the intraluminal thrombi. The so called thrombi, strongly positive for IgG and IgM with the immunofluorescence, appeared to be clusters of mononuclear cells which, for their ultrastructural and histochemical (nonspecific esterase activity) patterns have been considered as monocytes. They showed cytoplasmic vacuoles containing microtubular structures; the latter have been detected free in the capillary lumina and in the basement membranes as well. These structures have been considered as the ultrastructural counterpart of the IgG-IgM immune-complexes responsible of the nephropathy. Monocytes have been hypotesized to play a pathogenetical role, acting as cleaners of the cryoprecipitate from the capillary lumina.
38 citations
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TL;DR: In this article, a catalytic isomerization of 1-pentene to 2- cis and 2- trans -pentene with H 4 Ru 4 (CO) 12 in toluene at 70.4 °C is described.
38 citations
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TL;DR: The 13 C NMR apical carbon resonances for a series of Co 3 (CO) 9 CY complexes have been observed to be in the lowfiedl region (310-230 ppm).
35 citations
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TL;DR: It is suggested that the PK abnormality is not the cause of the haemolytic anaemia, but an epiphenomenon of a primary unknown defect that apparently involves the red cell thiol groups.
Abstract: The effect of some sulphydryl compounds on two new variants of red cell pyruvate kinase (ATP: pyruvate phosphotransferase, PK) is reported. In vitro a striking correction has been obtained of both the qualitative and, in one case, the quantitative defect of red cell PK. In vivo, a correction of the qualitative and quantitative abnormalities has been produced in both patients, with clinical improvement of one of them. These findings, together with the unexpected results in respect to the functional properties of PK found in the affected members of the two families studied, suggest that the PK abnormality is not the cause of the haemolytic anaemia, but an epiphenomenon of a primary unknown defect that apparently involves the red cell thiol groups.
33 citations
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TL;DR: Plasma gonadotropin pattern following exogenously administered luteinizing hormone-releasing hormone (LRH), orally administered for 8 to 35 months to 7 girls with idiopathic precocious puberty, was characterized before treatment by an exaggerated LH response; plasma FSH response was similar to prepubertal subjects.
Abstract: 50 mg daily of cyproterone acetate (CA) were orally administered for 8 to 35 months to 7 girls with idiopathic precocious puberty. Plasma levels of FSH, LH, cortisol, testosterone, estradiol, and progesterone were measured in 6 patients before treatment. Compared with control subjects of the same chronological age, significantly higher values were found for gonadotropins, testosterone, and estradiol. After treatment, no significant variation was observed in FSH and LH levels; testosterone was reduced in the majority of the cases without significant decline in mean values; estradiol fell significantly and returned in the prepubertal range. The plasma gonadotropin pattern following exogenously administered luteinizing hormone-releasing hormone (LRH, 100 μg iv) was characterized before treatment by an exaggerated LH response both in terms of maximum level (32.02 ± 4.35 se mlU/ml; prepubertal controls: 16.20 ± 1.45 se mlU/rnl) and maximum increment above baseline values (25.36 ± 2.84 se mlU/ml; prepubertal co...
29 citations
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TL;DR: In this paper, the electron transfer reaction between Fe(III) and several benzene-1,2-diols (namely, 4-methyl-, 4-t-butyl), 4-chloro-benzene, 1,2diol, l -Dopa and adrenaline) has been studied with a stopped-flow technique, at 25.0°C, μ = 1.0 M and 0.40 M.
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TL;DR: Catecholamines (adrenaline and L-Dopa) can be determined by a stopped-flow spectrophotometric technique that allows a catecholamine concentration to be determined in the presence of a ten-fold amount of another catechlamine, with a maximum error of about 10 %.
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TL;DR: In this paper, point group transformation of the LCAO CO basis functions is applied to confine the Roothaan equations within the irreducible part of the Brillouin zone.
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TL;DR: Electron spin resonance experiments showed that the temperatures of the breaks in the Arrhenius plots of some enzymatic activities in rat liver mitochondria coin- indicate the phase transition of lipids as detected by X-ray diffraction or thermal analysis.
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TL;DR: The primary aim of the present study was to investigate the possible effects on erythrocytes, blood platelets and capillary permeability of products of lipid peroxidation stimulated by CC14.
Abstract: Reaction products of lipid peroxidation have been found to affect both erythrocytes (Pfeifer & McCay, 1971) and blood platelets (Silver et al., 1973, 1974). Recent findings in our laboratory (Bini et al., 1975) suggest a possible pathogenic role for lipoperoxidation in producing severe systemic disturbances after a large dose of CCI4. Peroxidation of unsaturated fatty acids gives rise to the formation of various classes of reactive materials, including short-chain aldehydes (Schauenstein, 1967; Cobb & Day, 1965); malonaldehyde, for example, is a well-known derivative of peroxidative decomposition of polyunsaturated fatty acids. Further, prostaglandins are exceptionally reactive products of enzymic or non-enzymic reactions affecting lipoperoxides (Nutgeren et a/ . , 1966, 1967). The primary aim of the present study was to investigate the possible effects on erythrocytes, blood platelets and capillary permeability of products of lipid peroxidation stimulated by CC14. Male Wistar rats were used in all the experiments. Haemolysis was studied by incubating rat erythrocytes in vitro, prepared by the method of Pfeifer & McCay (1971). Erythrocytes (50pl equivalent) were incubated in the presence of 50mg equiv. of liver microsomal preparation and 1 mM-NADPH (Sigma Chemical Co., St. Louis, MO, U.S.A.) in a final volume of 1 ml. Where appropriate, the CCI, concentration was 60pI/ml. All the solutions were prepared with 0.05M-phosphate buffer, pH 7.4, containing 0.1 SSM-N~CI. n some instances, the following antioxidants or metabolites of microsomal mixed-function oxidase systems were added at final concentrations ranging between 0.6 and 3mM: NN'-diphenyl-p-phenylenediamine (Schuchardt, Munich, Germany), 2,6-di-t-butyl-pcresol (Fluka A.G., Buchs SG, Switzerland), Santoquin (6-ethoxy-l,2-dihydro-2,2,4trimethylquinoline; Monsanto Co., St. Louis, MO, U.S.A.), propyl gallate (Carlo Erba, Milano, Italy, DL-a-tocopherol (vitamin E; Merck, Darmstadt, Germany), Methyl Viologen (paraquat, 1,l '-dimethyl-4,4'-bipyridinium dichloride; Sigma), ergocalciferol (vitamin Dt ; Merck), aminopyrine (4-dimethylamin0-2,3-dimethyl-l phenyl-3-pyrazolin-5-one; Aldrich-Europe, Beerse, Belgium), codeine (codeine phosphate ; Macfarlane Smith Ltd., U.K.), SKF-525A (2-diethylaminoethyl-2,2-diphenyl-valerate hydrochloride; Smith, Klein and French Laboratories, Philadelphia, PA, U.S.A.), and/or GSH (reduced glutathione; Boehringer, Mannheim, Germany). The mixture was incubated in a Dubnoff apparatus at 37°C for 45min, then diluted with buffered saline to 5ml and centrifuged at 0°C for lOmin at 1OOOOg. Haemolysis was determined on the basis of the E545 of the clear supernatnat, and production of malonaldehyde was assayed on the same sample with thiobarbituric acid (Bieri &Anderson, 1960). Where haemolysis was to be related to either lipid peroxidation or the production of halogenated free radicals, the solution was mixed with 0.1 mM-EDTA and incubated under Nz by the procedure of Recknagel et al. (1975). Chloroform formation, detected quantitatively by a g.1.c. apparatus equipped with an electron capture detector (Fractovap, model GV; Carlo Erba), was interpreted as a sign of free-radical reactions occurring in the absence of fully developed lipid peroxidation. Direct haemolysis induced by lipoperoxides was followed by treating rat erythrocytes with linoleic acid hydroperoxide (prepared as described by O'Brien, 1969) as such or as a
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TL;DR: Salbutamol induced a reduction in glucose tolerance, in spite of the presence of enhanced blood insulin levels, and may be attributed to its β-adrenergic stimulating action.
Abstract: The effect of i.v. infusion of a beta-adrenergic stimulant, salbutamol on blood insulin, glucose, lactate, phosphates, potassium and NEFA was studied in 9 normal subjects. The effect of the drug on the blood sugar and insulin response to i.v. glucose was also examined in 4 subjects. Salbutamol was followed by increases in insulin, glucose and lactate, and a fall in blood phosphorus and potassium. NEFA values did not change significantly. These effects of salbutamol were abolished by propranolol and may thus be attributed to its beta-adrenergic stimulating action. Salbutamol also induced a reduction in glucose tolerance, in spite of the presence of enhanced blood insulin levels.
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TL;DR: A sensitive, solid-state, competitive radio immunological method for quantitation of antigens solubilized from membranes is described.
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TL;DR: It is discussed that the grasping reflex may be useful to characterize experimentally drugs with antidepressant properties.
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TL;DR: It is found that a significant amount of the labelled proteins are serum derivatives tightly bound to the membrane surface in the case of hamster fibroblasts cultured with calf serum, which implies that, when probe labelling of cell surface proteins is performed, the non-identity in acrylamide gel electrophoresis between labelled bands solubilized from cells and bands displayed by probelabelled native serum components is not a proper control to exclude surface adsorbtion.
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TL;DR: The ultrastructural organization of the spores of the sporocarp of Endogone flammicorona was studied and the so-called flaming crown was described, which gives the spore its most commonly observed morphological feature.
Abstract: The ultrastructural organization of the spores of the sporocarp of Endogone flammicorona was studied. Two types of organization are described. Initially the spore possessed a vacuolate protoplasm and was bound by two cell wall layers. The spore was surrounded by a hyphal mantle formed of a sheet of vacuolized hyphae with uniformly thin walls. Secondly, although the ultrastructural features of the spore appeared the same, it was now surrounded by a hyphal mantle with unevenly thickened walls (i. e., the so-called flaming crown) due to the gradual and irregular deposition of granules and lamellae. This crown gives the spore its most commonly observed morphological feature and is the preminent character employed taxonomically to speciate Endogone flammicorona Trappe & Gerdemann.
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TL;DR: Treatment for 11–13 weeks with a cholesterol-rich diet induced increases in free and esterified serum cholesterol and a rise in cholesterol to phospholipid ratio of plasma membranes occurred in cholesterol-enriched macrophages.
Abstract: Treatment for 11–13 weeks with a cholesterol-rich diet induced increases in free and esterified serum cholesterol. There was also an increase in free cholesterol of peritoneal macrophages. A 2.2 times rise in the cholesterol to phospholipid ratio of plasma membranes occurred in cholesterol-enriched macrophages. No changes were observed in phagolysosomes. Cholesterol-enriched macrophages showed a 35.8% inhibition of latex particles phagocytosis. When lipid droplets were substituted for latex the inhibition was 81.7%.
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TL;DR: Newborn mice have a lower spontaneous resistance to the growth of a syngeneic plasmacytoma (MOPC-460) as compared to adult mice, and the transfer of different leucocyte populations from non-immunized adult donors to newborn mice influence in a dual way the resistance to MOPc-460 growth, depending on the number of cells transferred.
Abstract: Newborn mice have a lower spontaneous resistance to the growth of a syngeneic plasmacytoma (MOPC-460) as compared to adult mice. The transfer of different leucocyte populations from non-immunized adult donors to newborn mice influence in a dual way the resistance to MOPC-460 growth, depending on the number of cells transferred. The transfer of a low number of neutrophils, thymus or spleen cells enhances the MOPC-460 takes. Higher numbers of neutrophils, thymus or bone marrow cells induce an effective protenction. By contrast, macrophages over a dose of 1 X 10(4) constantly produce a reduction of tumour growth.
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TL;DR: In this paper, a homogeneous isomerisation of 1-pentene to 2-cis-and 2-trans-pentenes occurs in the presence of dodecacarbonyltriosmium.
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TL;DR: The application of BCNU, CCNU and MeCCNU has been shown to greatly reduce the number of tumors induced in rats by transplacentally administered ENU and to inhibit the full development of tumor proliferation.
Abstract: The application of BCNU, CCNU and MeCCNU has been shown to greatly reduce the number of tumors induced in rats by transplacentally administered ENU and to inhibit the full development of tumor proliferation. Moreover, no neurinomas appeared after treatment with CCNU. The application of GCNU proved to be much less effective. Our statistics in the form of tables showing these findings are included and discussed.
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TL;DR: In this paper, the authors investigated the kinetics and mechanisms of the oxidation of 4,4′-biphenyldiol ([1,1′-cycloexadiene]-4,4-diol, I) to 4, 4-Biphenoquinone ([bi-2,5]-cyclohexadiene-(1, 1,1,cyclo exadiene)-1.1-ylidene)-4, 4-, dione, II] in perchlorate media at different temperatures and acidities.
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TL;DR: The following metabolites of the glycolytic pathway, along with ATP, ADP, AMP, citrate and inorganic phosphate, have been measured in the hepatic tissue of chick embryos and hatched chicks: glucose, glucose-6-phosphate, fructose-1,6-diph phosphate, dihydroxyacetone phosphate, glyceraldehyde-3-ph phosphate, phosphoenol-pyruvate, pyruvates and lactate.
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TL;DR: A clearcut pattern of changes has been observed with ATPase and oxidative enzymes with special changes known as “tubular aggregates” found on electron microscopy in congenital paramyotonia.
Abstract: In a case of congenital paramyotonia a muscle biopsy was performed and studied morphologically, histochemically and ultrastructurally. A clearcut pattern of changes has been observed with ATPase and oxidative enzymes. On electron microscopy special changes known as "tubular aggregates" were found. The relationship between the two findings, as well as the significance of such alterations in the range of periodic paralyses and myotonic phenomena, are discussed.