Showing papers by "University of Veterinary Science published in 2016"

Serological Survey and Factors Associated with Toxoplasma gondii Infection in Domestic Goats in Myanmar

Abstract: Goat farming is important for the livelihood of millions of rural people because it contributes to food security and creation of assets. However, infection of goats with Toxoplasma gondii could be a source of parasite transmission to humans. The information on T. gondii infection of goat was not reported yet in Myanmar. A total of 119 goat serum samples were collected from three cities in the central region of Myanmar for T. gondii antibody survey. With the occurrence value obtained in this first study, a second one, more complete, with larger number (162) of animals and properties, was carried out and the risk factors and prevalence were determined. In both studies the samples were analyzed by the LAT. Of these, 32 (11.4%) samples were showed to be positive. The infection was associated with the presence of cats at the farm (odds ratio [OR] = 4.66, 95% confidential interval [CI] = 1.03–21.06), farming with different animal species (sheep, cattle, and pigs) (OR = 4.33, 95% CI = 1.57–11.94), and farming without good management practices (OR = 0.23, 95% CI = 0.06–0.83). This is the first T. gondii prevalence study in goats in the country.

Effects of High Hydrostatic Pressure on Expression Profiles of In Vitro Produced Vitrified Bovine Blastocysts.

Abstract: High hydrostatic pressure (HHP) has been used to pre-condition embryos before essential, yet potentially detrimental procedures such as cryopreservation. However, the mechanisms for HHP are poorly understood. We treated bovine blastocysts with three different HHP (40, 60 and 80 MPa) in combination with three recovery periods (0, 1 h, 2 h post HHP). Re-expansion rates were significantly higher at 40 and 60 but lower at 80 MPa after vitrification-warming in the treated groups than controls. Microarray analysis revealed 399 differentially expressed transcripts, representing 254 unique genes, among different groups. Gene ontology analysis indicated that HHP at 40 and 60 MPa promoted embryo competence through down-regulation of genes in cell death and apoptosis, and up-regulation of genes in RNA processing, cellular growth and proliferation. In contrast, 80 MPa up-regulated genes in apoptosis, and down-regulated protein folding and cell cycle-related genes. Moreover, gene expression was also influenced by the length of the recovery time after HHP. The significantly over-represented categories were apoptosis and cell death in the 1 h group, and protein folding, response to unfolded protein and cell cycle in the 2 h group compared to 0 h. Taken together, HHP promotes competence of vitrified bovine blastocysts through modest transcriptional changes.

Intraspecific Phylogeny of the House Shrews, Suncus murinus-S. montanus Species Complex, Based on the Mitochondrial Cytochrome b Gene

Abstract: . A phylogenetic tree was reconstructed based on the mitochondrial cytochrome b gene nucleotide sequences of 169 individuals of house shrews (Suncus murinus and S. montanus) from 44 localities in East Asia, Southeast Asia, West Asia, and islands in the western Indian Ocean. Shrews from China (Zhejiang), Japan (Okinawa), Vietnam, and Indonesia (Java) formed a monophyletic group with less genetic variation. Therefore, the shrews of these regions appeared to have originated from one or a few localities. Contrary to this, shrews from Sri Lanka, Myanmar, and Pakistan consisted of several haplogroups. This finding suggests immigration movements to these areas. Fascinating findings were also obtained concerning the islands in the western Indian Ocean. First, shrews on Zanzibar Island (Tanzania) had almost the same haplotype as those in southwestern Iran. Therefore, the house shrew in Zanzibar may have immigrated from Iran (or vice versa). Second, shrews from Madagascar and Grande Comore Island shared the...

In vitro fermentation of conventional diets commonly fed to dairy cows in Central Myanmar

Abstract: This study consisted of two experiments conducted to evaluate the effective net gas, fermentation kinetics (experiment 1), methane gas concentration, partitioning factor (PF) for microbial protein synthesis and in vitro dry matter digestibility (IVDMD) (experiment 2) of conventional diets commonly fed to dairy cows in Central Myanmar. The conventional diets from four areas [diet from Sin Tel area (Diet-ST), diet from Myay Ngu area (Diet-MN), diet from Ta Pel area (Diet-TP) and diet from Amarapura area (Diet-AM)] were used as experimental diets in this study. In most of conventional diets, rice straw, sorghum stover and natural grass were used as roughage source and cotton seed cake and broken rice were used as concentrate. However in some diets, sesame residue and butter bean residue were used as roughage source instead of sorghum stover and natural grass. The roughage to concentrate ratio and crude protein (CP) content of conventional diets ranged from 53:47 to 72:28 and 11.46 to 17.96%, respectively. In experiment 1, the effective net gas volume of Diet-TP was lower (p<0.05) than Diet-ST and Diet-AM and generally, the fermentation kinetics (a, b, c and a+b) of Diet-TP were also lower than those of other diets. In the experiment 2, the lower value (p<0.05) of short chain fatty acid (SCFA) was found in Diet-MN and Diet-TP while the higher values (p<0.05) of metabolizable energy (ME) and organic matter digestibility (OMD) were observed Diet-AM and Diet-TP. Although methane gas concentration of Diet-TP was higher (p<0.05) than those of other diets, the greater values of IVDMD and PF were observed in Diet-TP. According to these findings, it was perceived that all conventional diets have different nutritional qualities which are useful for production and health of dairy cows; however the Diet-TP possessed the highest nutritional qualities among the conventional diets.

Feeding values of conventional diets and their effects on the performances of dairy cows in Central Myanmar

Abstract: The conventional diets have been fed to dairy cows since many years ago in Myanmar, however there is a little scientific information regarding the use of those diets as feed. Therefore, this study was conducted to evaluate the feeding values of conventional diets and their effects on the performances of dairy cows in Central Myanmar. Nine cross-bred Holstein Friesian cows (460±22kg) with the 12 th week of lactation were randomly allocated to one of three treatment groups with three replicates in a completely randomized design. The three treatments were Diet-ST (common diet from Sin Tel, Tatar U Township), Diet-MN (common diet from Myay Ngu, Tatar U Township) and Diet-AM (common diet from Amarapura Township). Cows were fed treatments for 60 days. The roughage to concentrate ratios ranged from 53:47 to 72:28 and the nutritive values were significantly different (p 0.05) except the CP and ether extract digestibility (CPD and EED). The nitrogen utilization was highest in dairy cows fed on Diet-ST and the lowest value was observed in the dairy cows fed on Diet-AM. Although the milk compositions were not different (p>0.05), the average milk yield of dairy cows offered the Diet-MN was significantly higher (p<0.05) than those of dairy cows fed on other diets. The highest total feed cost (p<0.05) was found in Diet-AM and the lowest value (p<0.05) was in Diet-MN. According to these findings, it was concluded that the highest feeding value was observed in Diet-MN and its effect on the performances of dairy cows was also greater than others. Moreover, it would be suggested that better bean residues and sesame residues could be used as fibre sources in the diets of dairy cows.

In situ nutrient degradation of conventional diets commonly fed to dairy cows in central Myanmar

Abstract: The determination of nutrient degradability is very important for the effective use of feeds in ruminant production Therefore, this experiment was conducted to determine the nutrient degradability and energy protein synchronization of conventional diets commonly fed to dairy cows in the central Myanmar through the in situ method The conventional diets are Diet-ST (diet from Sin Tel), Diet-MN (diet from Myay Ngu), Diet-TP (diet from Ta Pel) and Diet-AM (diet from Amarapura) The roughage to concentrate ratio of conventional diets ranged from 53:47 to 72:28 The crude protein (CP) content of Diet-TP (1796%) was significantly higher (p<005) than those of other diets with the least CP obtained from Diet-AM (1146%) Among the conventional diets, wide variation of dry matter (DM) and organic matter (OM) disappearances were observed throughout the incubation times The highest value (p<005) was found in Diet-AM and the lowest (p<005) was observed in Diet-ST and Diet-TP The rapidly degradable fraction “a” and rate of degradation “c” of DM and OM of Diet-AM was significantly higher (p<005) than those of other diets The effective degradability of DM and OM were also highest (p<005) in Diet-AM Generally, the lowest (p<005) CP disappearance was observed in Diet-TP The rapidly degradable fraction “a” of CP was highest (p<005) in Diet-ST and the greater (p<005) effective degradability was observed for Diet-AM Moreover, the highest values (p<005) of rumen degradable protein (RDP), rumen undegradable protein (UDP) and energy protein synchronization were found in Diet-TP, while the lowest value was observed in Diet-AM Therefore, it was concluded that all conventional diets could be used as ration for dairy cows; however, the best potential was found in Diet-AM because of its highest effective degradability of nutrients

Effect of leucaena forage and silage substitution in concentrates on digestibility, nitrogen utilization and milk yield in dairy cows

Abstract: This experiment was conducted to determine the effect of feeding leucaena forage and silage substitution in concentrate on the performances of dairy cows. Nine cross-bred Holstein Friesian cows (410±12kg) in the 12 th week of lactation were randomly allocated to one of three treatment groups with three replicates/treatments in a completely randomized design. The three treatments were control diet without substitution of leucaena forage and silage (DLFS0), diet with substitution of leucaena forage 10% (DLF10) and diet with substitution of leucaena silage 10% (DLS10). Cows were fed treatments for 60 days. Although nutrient intakes were not significantly different (p>0.05) each other, digestibility of DLFS0 was significantly higher (p<0.05) than others. Conversely, nitrogen utilization and average milk yield of cows offered DLFS0 were significantly lower (p<0.05) than those of cows fed on DLF10 and DLS10. The highest feed cost (p<0.05) per kg of milk was found in DLFS0 and the lowest cost was observed in DLF10. Therefore, although the leucana forage and silage could be substitute up to 10% of concentrates without adverse effects on the performances of dairy cows, the substitution of leucaena forage gave the better performances than that of leucaena silage.

Survey of the Porcine Trichinellosis in Slaughtered Pigs in Nay Pyi Taw, Myanmar

Abstract: A cross-sectional study was conducted to determine the prevalence and associated risk factors of trichinellosis in slaughtered pigs from three slaughterhouses located in Pyinmana, Lewe and Tatkon Townships, Nay Pyi Taw, Myanmar. A total of 270 muscle samples (tongue, masseter and diaphragmatic muscles from each pig) from 90 slaughtered pigs were randomly collected and examined for the presence of Trichinella larvae by using artificial digestion method. Three pigs (3.3%) showed Trichinella positive and larval intensity per gram (lpg) was 0.2. For the determination of associated risk factors of trichinellosis, questionnaire interviews to officials of slaughterhouses and owners of slaughtered pigs were conducted. The presence of rodents around the pig farm and type of feed (use of uncooked waste food) were identified as associated risk factors for trichinellosis. Porcine trichinellosis occurred with a low rate of infection in this study.