University of Veterinary Science

About: University of Veterinary Science is a education organization based out in Pyinmana, Myanmar. It is known for research contribution in the topics: Population & Feed conversion ratio. The organization has 597 authors who have published 650 publications receiving 14262 citations.

Sonographic diagnosis of intestinal obstruction in the dog.

Abstract: Ultrasonography was performed on 44 dogs to decide whether small bowel obstruction was present. The sonographic criteria for small bowel obstruction were (1) the presence of pendulous movement of the ingesta inside the dilated bowel, (2) observation of invaginated intestines or an ingested intraluminal foreign body, (3) observation of non-uniform peristaltic activity of the dilated intestines, or (4) observation of akinetic intestinal loops together with abdominal fluid accumulation. By using these criteria, obstruction was correctly diagnosed by ultrasonography in 11 of the 13 dogs with mechanical ileus, and obstruction was correctly excluded in 29 of the 31 non-obstructive cases. Thus, the above-mentioned sonographic criteria had 85% sensitivity and positive predictive value, and 94% specificity and negative predictive value. The present study suggests that ultrasonography is a valuable tool for diagnosing small intestinal obstruction in the dog.

Induced lactation with a dopamine antagonist in mares: different responses between ovariectomized and intact mares.

Abstract: The aim of this study was to compare the effects of treatment with repeated injections of sulpiride (a dopamine D2 antagonist) on prolactin secretion and induced lactation in ovariectomized and intact adult mares and to verify if this induction was possible at the beginning and at the end of the birth season. Two experiments were carried out in September [experiment (expt) 1], and in March (expt 2), in France (48� N). In expt 1, three groups of five mares were tested: intact-control, intact-treated and ovariectomized-treated mares. In expt 2, mares previously subjected to artificial photoperiod were assigned in two groups: four intact-control and five intact-treated mares. The cyclicity of intact mares was previously synchronized with PGF2a injections, then all the mares were in the follicular phase at the beginning of treatment. Sulpiride was intramuscularly injected (0.5 mg/kg of BW), twice a day. Mares were milked at 7:30, 11:45, 16:00 and 20:15 hours. Blood samples were collected every day during the treatment for progesterone, total oestrogen and prolactin assays. In the two experiments, only treated intact mares produced milk, with a large inter-animal variability. Prolactin increase after sulpiride treatment was not so great in the ovariectomized-treated mares as in the intact-treated mares. The total correlations between prolactin, progesterone, oestrogen plasma concentrations and daily milk production were significant (0.57, 0.25, 0.17 respectively). This induction of lactation can be performed during the entire birth season in intact mares, but not in ovariectomized mares, indicating that steroids are necessary for this induction in mares treated by dopamine D2 antagonist.

Quantitative method to assess Cryptosporidium oocyst shedding in the chicken model

Abstract: An unsophisticated and fairly sensitive quantitative method and its grounds are described, which proved useful to assessCryptosporidium oocyst shedding in the chicken model. The method is based on the rather slow sedimentation of oocysts. A threshold of sensitivity ranging between 5,000 and 10,000 oocysts/g feces was established for this technique. There was good agreement between triplicate assays over a wide range of oocyst concentrations.

Comparison of 3H-thymidine incorporation and celltiter 96™ aqueous colorimetric assays in cell proliferation of bovine mononuclear cells

Abstract: A rapid colorimetric non-radioactive assay for the determination of bovine mitogen-induced lymphocyte proliferation in cell culture was evaluated using a novel tetrazolium compound (MTS) and an electron coupling reagent (PMS) provided in the CellTiter 96 kit (Promega). The results of the new method were compared with those of the 3H-thymidine incorporation assay using parallels obtained from the same lymphocyte population. The concentrations used in the cell suspension of primary cultured lymphocytes resulted in a significant signal/background ratio when cells were prepared from peripheral blood, spleen or mesenteric lymph nodes. The same concentrations of thymocytes resulted in a weak signal even for the highest concentrations of mitogen. A good correlation was demonstrated between the results of the two methods. The non-radioactive method performed well in assays in bovine mononuclear cells derived from prolactin-treated or -untreated calves, showing a 50% lower responsiveness to mitogenic stimulation in prolactin-deprived animals.

Coagglutination test for serotyping Pasteurella haemolytica.

Abstract: A coagglutination test was described for simple, fast, and reliable detection of Pasteurella haemolytica type-specific antigens in lung lesions even in the absence of viable P. haemolytica. The coagglutinating reagents were prepared by coating protein A-producing Staphylococcus aureus cells with hyperimmune sera raised against P. haemolytica type strains. Bacterial suspensions, saline extracts, and boiled saline extracts of the bacteria were used as antigens. Homologous reactions with all types of antigens were precise. Some cross-reactions were similar to those obtained by the indirect hemagglutination test, and some additional one-way cross-reactions were identified. The coagglutination test was used for serotyping 65 P. haemolytica field strains and for the detection of P. haemolytica type-specific antigens in the lung specimens of 62 calves and 78 sheep. Ninety-four percent of the field strains could be serotyped by the coagglutination test. P. haemolytica type-specific antigens were detected in the lung specimens of 3 calves and 5 sheep that had succumbed to naturally occurring P. haemolytica pneumonia and in the lungs of 20 calves experimentally infected with P. haemolytica A1. The coagglutination test detected type-specific antigens in 36% of the lung specimens of slaughtered field sheep but not in the lungs of slaughtered field cattle with small chronic lung lesions. No reaction occurred in the case of nonpneumonic calves and sheep or when pneumonic lesions were caused by other bacteria. No P. haemolytica strains could be isolated from lung samples that were coagglutination test negative. This test is recommended as an additional method for fast and reliable serotyping of P. haemolytica.