University of Veterinary Science

About: University of Veterinary Science is a education organization based out in Pyinmana, Myanmar. It is known for research contribution in the topics: Population & Feed conversion ratio. The organization has 597 authors who have published 650 publications receiving 14262 citations.

Immunohistochemical detection of bovine ruminal carbonic anhydrase isoenzyme.

Abstract: Rabbits immunized with low-activity ruminal carbonic anhydrase (RCA) isoenzyme, extracted from ruminal epithelial cells isolated by digestion with trupsin, yielded anti-RCA sera which reacted specifically with bovine RCA in double agar gel diffusion and immunoelectrophoretic tests, but failed to cross-react with bovine erythrocyte CA. The localization of RCA was identified in histological sections and isolated ruminal epithelial cell preparations by indirect immunofluorescence and immunoperoxidase tests as the basal, spinosum and granulosum layers of ruminal mucous epithelium.

Effect of different concentrations of ascorbic acid on motility, membrane integrity and chromatin status of frozen-thawed canine spermatozoa within six hours of storage at 37°C.

Abstract: The aim of this study was to examine comprehensively the effect of ascorbic acid (Asc) on frozen-thawed canine semen. Pooled ejaculates (n = 10) were assessed for quality with a computer-assisted sperm analyser (CASA). After centrifugation, the semen was divided into four aliquots (A-D) of which three were diluted with Uppsala 1 extender (v/v) containing different concentrations of Asc (B: 6.8 mmol/l; C: 13 mmol/l; D: 27 mmol/l). One group without Asc served as control (A). Subsequently, dilution samples were treated and cryopreserved as described previously (Theriogenology 66, 2006, 173). After thawing, samples were stored at 37 degrees C for 1, 3 and 6 h, then examined for quality (CASA, flow cytometry, sperm chromatin structure assay; SCSA). Staining for flow cytometry was performed with FITC-PNA and propidium iodide (Reproduction 128, 2004, 829). The SCSA was performed with both Tris-NACL-EDT buffer (TNE)- and Tris-fructose-citrate buffer (TFC). In the Asc-supplemented groups, percentages of progressively motile sperm (P) were significantly lower than in the control group (A 1 h: 56.6 +/- 9.8, 6 h: 18 +/- 4.5; B 1 h: 51.2 +/- 11.8, 6 h: 13.6 +/- 5; C 1 h: 41 +/- 16.2, 6 h: 11.2 +/- 6.1; D 1 h: 37 +/- 15.2, 6 h: 8.6 +/- 5; p 0.05). Furthermore, there were no significant differences between TNE- and TFC-samples, for alphaT, for SD of alphaT or for comp alphaT [comp alphaT: TCF-A: 2.5 +/- 1.7%, TNE-A: 3.4 +/- 3.2%; TCF-D: 2.5 +/- 2%, TNE-D: 3.3 +/- 4.3%; p > 0.05]. However, samples diluted with both extenders correlated concerning alphaT, but not comp alphaT. We therefore recommend using TNE-buffer for SCSA with cryopreserved canine semen. In addition, the average alphaT values did not differ significantly between the controls and all other groups (TNE-A: 380.2 +/- 89.1; TNE-D: 338.1 +/- 137.4; p > 0.05). It can be concluded that addition of Asc to cryoextender does not increase quality of frozen-thawed canine semen.

Readability and histological biocompatibility of microchip transponders in horses.

Abstract: Identification of horses by microchip transponder is mandatory within the European Union with only a few exceptions. In this study, the readability of such microchips in 428 horses with three different scanners (A, B and C) and the histological changes at the implantation site in 16 animals were assessed. Identification of microchips differed between scanners (P<0.001), and with 'side of neck' (P<0.001). Scanners A, B and C identified 93.5%, 89.7% and 100% of microchips, respectively, on the 'chip-bearing' side of the neck. From the contralateral side, scanners A, B and C identified 21.5%, 26.9% and 89.5% of transponders, respectively. Microchip readability was affected by age (P<0.001), but not by breed of horse. At necropsy, transponders were found in the subcutaneous fat (n=3), inter- or peri-muscular connective tissue (n=8), or musculature (n=5), where they were surrounded by a fibrous capsule ranging in thickness from 12.7 to 289.5 μm in 15 animals. In two animals, immature granulation tissue with attendant granulomatous inflammation, and a granulomatous myositis, surrounding the microchip were identified, respectively. Severe (n=1), moderate (n=1), and mild (n=3) lymphohistiocytic inflammation was noted within the fibrous capsule. Microchip transponders were found to be a highly reliable and biocompatible method of horse identification.

Induction of acrosome reaction in dog sperm by calcium ionophore

Abstract: The sensitivity of the plasma membrane to calcium ionophore (A23187) challenge was studied in dog sperm using fluorescein lectin staining for the assessment of acrosomal status and viability Second fraction ejaculates from 5 dogs were washed, resuspended in Ca(2+)-free (EDTA-treated), 50, 100, 500, 1000 and 2000 microM/l Ca(2+)-containing Sp-TALP medium and induced with 50, 250, 500, 1000, 2500 and 5000 nM/l calcium ionophore Samples were collected from each aliquot after 30 and 60 min of induction to assess the percentage of acrosome reacted sperm cells (AR rate), viability and motility by fluorescein isothiocyanate conjugated peanut agglutinin (FITC-PNA) and ethidium-homodimer combined staining On each slide, 200 sperm cells were assessed under epifluorescence microscope (x 1250) in a blind manner The response to ionophore challenge (AR rate, viability, motility) varied with Ca2+ and ionophore concentration in the suspension A significantly higher AR rate was detected in samples containing 100, 500, 1000 and 2000 microM/L Ca2+ (> 40%) than in that containing 50 microM/L Acrosome reaction could not be successfully induced in the EDTA-treated sample and in any of the aliquots in which 50, 250 and 500 nM/L ionophore concentrations were used for induction Motility decreased drastically in all of the treated samples and stopped in that sample where as significant AR rate could be detected Viability remained high (> 75%) during the incubation and did not differ significantly in the treated and the control groups