University of Veterinary Science

About: University of Veterinary Science is a education organization based out in Pyinmana, Myanmar. It is known for research contribution in the topics: Population & Receptor. The organization has 597 authors who have published 650 publications receiving 14262 citations.

Sensitive ELISA Test for Determination of Ochratoxin A

Abstract: A direct, competitive enzyme-linked immunosorbent assay (ELISA) with monoclonal antibody has been developed for quantitative determination of ochratoxin A (OA) in different cereals. A dichloromethane/citric acid mixture was used for extraction of cereals. This cleanup procedure proved to be as effective for ochratoxin A extraction as protocols using strong acids. The mean within-assay and interassay coefficients of variation for the standard curve was <10%. The range of this test is 1−10 ng/g, with a detection limit of 0.5 ng/g OA. The toxin recovery from cereals infected with 5−100 ng/mL OA varied between 90 and 130%. Keywords: Ochratoxin A; ELISA; cereals

Application of techniques for sperm selection in fresh and frozen-thawed stallion semen.

Abstract: The objective of this research was to improve the techniques in processing chilled and frozen-thawed horse semen. In a preliminary experiment (Exp. I), different techniques for sperm selection and preparation [Swim-up, Glass wool (GW) filtration, Glass wool Sephadex (GWS) filtration; Percoll] were tested for their suitability for equine spermatozoa and results were compared with the routine procedure by dilution (Exp. I). In the main experiment (Exp. II), two sperm preparation techniques (GWS, Leucosorb) refering to the results of Exp. I and a previous study of our group (Pferdcheilkunde 1996 12, 773) were selected for processing complete ejaculates either for cooled-storage or cryopreservation. In a third experiment (Exp. III), pregnancy rates from inseminations with semen processed according to the techniques tested in Exp. II were compared with those obtained with semen processed according to routine procedures. In Exp. I (six stallions, six ejaculates/stallion), between 48 and 92% of spermatozoa were lost following the different sperm selection procedures (p < 0.05). Preparation of sperm increased percentage of progressively motile spermatozoa (pms) [Swim-up, GW, GWS vs dilution, Percoll (p < 0.05)] and decreased percentage of sperm head abnormalities [Swim-up, GW, GWS vs dilution, Percoll (p < 0.05)] probably by not improving the quality of individual cells, but by elimination of spermatozoa of inferior quality. In Exp. II (eight stallions, three ejaculates/stallion) Leucosorb and GWS procedures allowed the filtration of large volumes (extended ejaculates) for routine laboratory practice. GWS and Leucosorb filtration resulted in increased motility, membrane integrity and sperm viability after storage of spermatozoa until 48 h at +5 degrees C when compared with control (diluted) and centrifuged semen (p < 0.05). Significantly more spermatozoa were recovered after centrifugation (87.8 +/- 15.4%) compared with GWS (63.5 +/- 18.6%) and Leucosorb filtration (53.6 +/- 22.3%). GWS or Leucosorb procedure resulted in successful cryopreservation of stallion semen without centrifugation for removal of seminal plasma. The per cycle conception rate of inseminated mares using 200 x 106 pms transferred within 8 h after collection of semen was not affected by GWS filtration or Leucosorb separation when compared with centrifugation (n.s.; Exp. III). In conclusion, GWS and Leucosorb filtration results in the improvement of semen quality and should be considered as a method for stallion semen processing. Additional studies are needed for the evaluation of potentially higher fertilizing ability of stallion spermatozoa separated by techniques for sperm selection.

Fifth European Dirofilaria and Angiostrongylus Days (FiEDAD) 2016

Abstract: A1 Human dirofilariosis in Europe: basic facts and retrospective review F Simón, V Kartashev, J González-Miguel, A Rivera, A Diosdado, PJ Gómez, R Morchón, M Siles-Lucas Laboratory of Parasitology, Faculty of Pharmacy, University of Salamanca, Salamanca, 37007, Spain; Rostov State Medical University, Rostov-na-Donu, 344022, Russia; North Caucasus Research Veterinary Institute, Novocherkassk, 346421, Russia; Laboratory of Parasitology, IRNASA, CSIC, Salamanca, 37008, Spain Correspondence: F Simón (fersimon@usal.es) Parasites & Vectors 2016, 10(Suppl 1):A1