University of Veterinary Science

About: University of Veterinary Science is a education organization based out in Pyinmana, Myanmar. It is known for research contribution in the topics: Population & Receptor. The organization has 597 authors who have published 650 publications receiving 14262 citations.

The value of different serological tests in serotyping of Actinobacillus pleuropneumoniae strains.

Abstract: Immune sera were raised in rabbits against 12 serotypes of Actinobacillus pleuropneumoniae biotype I in order to compare serological tests for simplicity and sensitivity in serotype determination. The serological tests included the tube agglutination test with and without 2-mercaptoethanol addition, the indirect haemagglutination test with and without 2-mercaptoethanol, the immunodiffusion test and the coagglutination test. All these tests proved suitable for serotype determination with the reference strains; however, cross-reactions of varying degree were observed in all of them. Owing to its simplicity, rapidity and specificity, the coagglutination test appears to be the most suitable for primary serotyping. A more definite answer to the question could be obtained by serotyping strains isolated from field cases.

Ethanol's effect on rat pituitary adrenal axis is prevented by purine metabolic pathway inhibitors.

Abstract: This study investigated the in vitro dose effects of ethanol (EtOH), adenosine (ADO), and urate (URA) on the basal and CRF stimulated ACTH production of pituitary tissue culture (PTC). Furthermore, the effects of low (LE = 0.5 gkg bodyweight) and high (HE = 2.5 gkg bodyweight) concentrations of EtOH were tested in rats on plasma ACTH and corticosterone concentration (PCC), with or without the following ADO metabolic pathway inhibitors: 6-mercaptopurine (6MP) (salvage pathway) and purpu- rogallin (PPG) (xanthine dehydrogenase). M) stimulated ACTH secretion in PTC; in fact doses up to 20 mM tended to be inhibitory. ADO significantly increased only basal ACTH secretion whereas URA increased both basal and CRF-stimulated ACTH secretion. Pretreatment with PPG or 6MP + PPG significantly increased both in vivo ACTH and PCC over control values in rats. HE versus LE significantly increased ACTH and PCC in the control (H,O) and 6MP pretreated groups whereas in the PPG pretreated animals, only ACTH was increased significantly by HE. However, combined pretreat- ment with 6MP + PPG prevented the effect of HE on ACTH and PCC. The current experiment suggests that purine metabolism is involved in ethanol's effect on the hypothalamic-pituitary-adrenal axis. (P.S.E.B.M. 1999, Vol 2201 EtOH at 0-50 mM does not increase the in vitro basal or CRF igh doses of ethanol (EtOH) in vivo have been shown to increase the production of adrenocortico- H trophic hormone (ACTH) and plasma corticoste- rone (PCC) concentrations, whereas lower doses and chronic consumption have more varied effects (1-3). In vitro, the acute effect of ethanol has been reported to increase (4), or leave unaltered spontaneous and CRF- stimulated ACTH release from pituitary cells, whereas pre- treatment with EtOH for 24 hr decreased both the sponta- neous and stimulated ACTH secretion (5). These varied effects of ethanol on ACTH secretion may be due to the

Analysis of the equalization of inverted repeats and neurovirulence using a pseudorabies virus mutant strain altered at the Ul/Ir junction.

Abstract: A recombinant pseudorabies (Aujeszky’s disease) virus (PrV) designated as vE16lac was constructed by deleting a 3-kbp DNA segment spanning the junction of long and short components of the viral genome, and by replacing the deleted segment with a lacZ-expression cassette. The aim of constructing this mutant was (a) to determine whether the terminal repeat (Tr) can serve as a template for the regeneration of the internal repeat (Ir), and (b) whether this deletion causes a reduction in the neuroinvasiveness of the virus. To analyze the mechanism of equalization, revertant viruses were selected and structurally characterized from vE16lac infection of PK-15 cells, mice and pigs. Because all revertants acquired Ir sequences identical to that of the wild-type virus, the equalization process occurred using the Tr as a template to reconstitute the Ir. We also found that the recombinant virus vE16lac was virulent in both pigs and mice. The data are discussed in view of studies performed with similar PrV mutants by other authors (Rall et al., 1992, Dean and Cheung, 1995 and Dean et al., 1996).

Antigenic relationship between human and animal adenovirus hexons determined by means of monoclonal antibodies directed against bovine adenovirus type 2 hexon

Abstract: Eight monoclonal antibodies (MAbs) directed against bovine adenovirus (BAV) 2 subtype B hexon were studied with 12 different hexon types of human adenoviruses (AV) belonging to 5 different subgenera using indirect ELISA, passive hemagglutination (HA), and gel diffusion assays. Two hexon types of animal origin (BAV3 and SAV16) were investigated, too. The reactivity of the MAb IV.F3 was the broadest, i.e. in ELISA and HA experiments it reacted with all hexon types studied. Based on these results as well as on the results of gel diffusion assays, this MAb should recognize the genus specific epitope of adenovirus hexons. Three MAbs (CA12, III.B11, and A12) could recognize different epitopes showing intersubgenus or intertype specificities. In spite of the fact, that all the eight MAbs proved to be bound by the hexon of ORT/111 (BAV2 subtype B) blotted onto nitrocellulose filter, four of the eight MAbs (BB7, BH5, II.A9, and IV.F5) failed to react with any human, and animal hexon types used in the present experiments. The results suggest that a gradient of antigenic relationship may exist between BAV2 hexon and the hexons of human serotypes.