Showing papers by "University of Vienna published in 1997"

The Convenient Setting of Global Analysis

Abstract: Introduction Calculus of smooth mappings Calculus of holomorphic and real analytic mappings Partitions of unity Smoothly realcompact spaces Extensions and liftings of mappings Infinite dimensional manifolds Calculus on infinite dimensional manifolds Infinite dimensional differential geometry Manifolds of mappings Further applications References Index.

A new rank based version of the ant system: a computational study

Abstract: The ant system is a new meta-heuristic for hard combinatorial optimization problems. It is a population-based approach that uses exploitation of positive feedback as well as greedy search. It was first proposed for tackling the well known Traveling Salesman Problem (TSP), but has been also successfully applied to problems such as quadratic assignment, job-shop scheduling, vehicle routing and graph coloring.In this paper we introduce a new rank based version of the ant system and present results of a computational study, where we compare the ant system with simulated annealing and a genetic algorithm on several TSP instances. It turns out that our rank based ant system can compete with the other methods in terms of average behavior, and shows even better worst case behavior. (author's abstract)

Range of antinuclear antibodies in "healthy" individuals

Abstract: Objective. To determine the range of antinuclear antibodies (ANA) in “healthy” individuals compared with that in patients with systemic lupus erythematosus (SLE), systemic sclerosis (SSc; scleroderma), Sjogren's syndrome (SS), rheumatoid arthritis (RA), or soft tissue rheumatism (STR). Methods. Fifteen international laboratories experienced in performing tests for ANA by indirect immunofluorescence participated in analyzing coded sera from healthy individuals and from patients in the 5 different disease groups described above. Except for the stipulation that HEp-2 cells should be used as substrate, each laboratory used its own in-house methodology so that the data might be expected to reflect the output of a cross-section of worldwide ANA reference laboratories. The sera were analyzed at 4 dilutions: 1:40, 1:80, 1:160, and 1:320. Results. In healthy individuals, the frequency of ANA did not differ significantly across the 4 age subgroups spanning 20–60 years of age. This putatively normal population was ANA positive in 31.7% of individuals at 1:40 serum dilution, 13.3% at 1:80, 5.0% at 1:160, and 3.3% at 1:320. In comparison with the findings among the disease groups, a low cutoff point at 1:40 serum dilution (high sensitivity, low specificity) could have diagnostic value, since it would classify virtually all patients with SLE, SSc, or SS as ANA positive. Conversely, a high positive cutoff at 1:160 serum dilution (high specificity, low sensitivity) would be useful to confirm the presence of disease in only a portion of cases, but would be likely to exclude 95% of normal individuals. Conclusion. It is recommended that laboratories performing immunofluorescent ANA tests should report results at both the 1:40 and 1:160 dilutions, and should supply information on the percentage of normal individuals who are positive at these dilutions. A low-titer ANA is not necessarily insignificant and might depend on at least 4 specific factors. ANA assays can be a useful discriminant in recognizing certain disease conditions, but can create misunderstanding when the limitations are not fully appreciated.

Disjunctive datalog

Abstract: We consider disjunctive Datalog, a powerful database query language based on disjunctive logic programming. Briefly, disjunctive Datalog is a variant of Datalog where disjunctions may appear in the rule heads; advanced versions also allow for negation in the bodies which can be handled according to a semantics for negation in disjunctive logic programming. In particular, we investigate three different semantics for disjunctive Datalog: the minimal model semantics the perfect model semantics, and the stable model semantics. For each of these semantics, the expressive power and complexity are studied. We show that the possibility variants of these semantics express the same set of queries. In fact, they precisely capture the complexity class ΣP2. Thus, unless the Polynomial Hierarchy collapses, disjunctive Datalog is more expressive that normal logic programming with negation. These results are not only of theoretical interest; we demonstrate that problems relevant in practice such as computing the optimal tour value in the Traveling Salesman Problem and eigenvector computations can be handled in disjunctive Datalog, but not Datalog with negation (unless the Polynomial Hierarchy collapses). In addition, we study modularity properties of disjunctive Datalog and investigate syntactic restrictions of the formalisms.

Proteolytic activation of tick-borne encephalitis virus by furin.

Abstract: Flaviviruses are assembled intracellularly in an immature form containing heterodimers of two envelope proteins, E and prM. Shortly before the virion exits the cell, prM is cleaved by a cellular enzyme, and this processing step can be blocked by treatment with agents that raise the pH of exocytic compartments. We carried out in vivo and in vitro studies with tick-borne encephalitis (TBE) virus to investigate the possible role of furin in this process as well as the functional consequences of prM cleavage. We found that prM in immature virions can be correctly cleaved in vitro by recombinant bovine furin but that efficient cleavage occurs only after exposure of the virion to mildly acidic pH. The data suggest that exposure to an acidic environment induces an irreversible structural change that renders the cleavage site accessible to the enzyme. Cleavage by furin in vitro resulted in biological activation, as shown by a 100-fold increase in specific infectivity, the acquisition of membrane fusion and hemagglutination activity, and the ability of the envelope proteins to undergo low-pH-induced structural rearrangements characteristic of mature virions. In vivo, prM cleavage was blocked by a furin inhibitor, and infection of the furin-deficient cell line LoVo yielded only immature virions, suggesting that furin is essential for cleavage activation of flaviviruses.

Inflammatory central nervous system demyelination: Correlation of magnetic resonance imaging findings with lesion pathology

Abstract: Magnetic resonance imaging (MRI) is widely used to evaluate and monitor disease activity in inflammatory demyelinating central nervous system (CNS) diseases such as multiple sclerosis. The present study aimed at correlating MRI findings with histological parameters in 6 cases of biopsy-proven inflammatory demyelination of the CNS. The earliest stages of demyelinating activity manifested as almost isointense lesions with a massive gadolinium-DTPA (Gd-DTPA) enhancement in T1-weighted scans. In T2-weighted scans, early active lesions formed a border of decreased intensity compared with the lesion center and the perifocal edema. The morphological correlate of this pattern in our patients was activated macrophages in the zone of myelin destruction at the plaque border. Late active lesions were hypointense in T1 and hyperintense in T2 scans. Inactive demyelinated and remyelinating lesions were hyperintense in T2 scans and enhanced inhomogenously after Gd-DTPA application. T1 scans revealed major differences in the degree of hypointensity that correlated with the extent of axonal damage, extracellular edema, and the degree of demyelination or remyelination.

Podoplanin, novel 43-kd membrane protein of glomerular epithelial cells, is down-regulated in puromycin nephrosis.

Abstract: Puromycin aminonucleoside nephrosis (PAN), a rat model of human minimal change nephropathy, is characterized by extensive flattening of glomerular epithelial cell (podocyte) foot processes and by severe proteinuria For comparison of expression of glomerular membrane proteins of normal and PAN rats, a membrane protein fraction of isolated rat glomeruli was prepared and monoclonal antibodies were raised against it An IgG-secreting clone designated LF3 was selected that specifically immunolabeled podocytes of normal but not of PAN rats The antigen of LF3 IgG was identified as a 43-kd glycoprotein Molecular cloning of its cDNA was performed in a delta gt11 expression library prepared from mRNA of isolated rat glomeruli The predicted amino acid sequence indicated a 166-amino-acid integral membrane protein with a single membrane-spanning domain, two potential phosphorylation sites in its short cytoplasmic tail, and six potential O-glycosylation sites in the large ectodomain High amino acid sequence identities were found to membrane glycoproteins of rat lung and bone and mouse thymus epithelial cells as well as to a phorbol-ester-induced protein in a mouse osteoblast cell line and to a canine influenza C virus receptor In PAN, expression of this 43-kd protein was selectively reduced to < 30%, as determined by quantitative immunogold electron microscopy, immunoblotting, and Northern blotting These data provide evidence that transcription of the 43-kd transmembrane podocyte glycoprotein is specifically down-regulated in PAN To indicate that this protein could be associated with transformation of arborized foot processes to flat feet (Latin, pes planus) we have called it podoplanin

An IR absorption calibration for water in minerals

Abstract: Using IR absorption data from polarized measurements on single-crystal minerals with stoichiometric water contents (in the form of H_(2)O or OH groups in the structure), a linear calibration curve (r^2 nearly equal 0.98) for water in minerals is established in the form: epsilon i , (the integrated molar absorption coefficient in units of cm^(-2) per mol H_(2)O/L) = 246.6(3753 - upsilon ) (upsilon = the mean wavenumber of the OH stretching band [in cm^(-1)]). The investigated minerals include hydrogrossular, analcime, hemimorphite and its dehydrated phase, lawsonite, goethite, diaspore, manganite, mozartite, and pectolite. The influence of hydrogen bonding, leading to increased absorption values with lower OH stretching band energies, is confirmed. It is further shown that only the use of integrated absorbance values (band areas) results in a linear correlation with water content, whereas linear absorption data (peak heights) are not correlated. The calibration agrees with previously published quantitative IR data on staurolite and trace H in pyroxenes. It is also close to the frequently used trend of Paterson (1982). However, some of the previous calibrations of trace H in nominally anhydrous minerals, e.g., kyanite and pyrope, differ appreciably from the correlation derived from stoichiometrically hydrous minerals.

Ultrasonographic Guidance Improves Sensory Block and Onset Time of Three-in-One Blocks

Abstract: The use of ultrasound reduces the onset time, improves the quality of sensory block, and minimizes the risks associated with the supraclavicular approach for brachial plexus and stellate ganglion blockade. The present study was designed to evaluate whether ultrasound also facilitates the approach for 3-in-1 blocks. Forty patients (ASA physical status II or III) undergoing hip surgery after trauma were randomly assigned to two groups. In the ultrasound (US) group, 20 mL bupivaCaine 0.5% was administered under US guidance, whereas in the control group, the same amount and concentration of local anesthetic was administered with the assistance of a nerve stimulator (NS). After US- or NS-based identification of the femoral nerve, the local anesthetic solution was administered, and the distribution of the local anesthetic solution was visualized and recorded on videotape in the US group. The quality and the onset of the sensory block was assessed by using the pinprick test in the central sensory region of each of the three nerves and compared with the same stimulation on the contralateral leg every 10 min for 60 min. The rating was performed using a scale from 100% (uncompromised sensibility) to 0% (no sensory sensation). Heart rate, noninvasive blood pressure, and oxygen saturation were measured at short intervals for 60 min. The onset of sensory blockade was significantly shorter in Group US compared with Group NS (US 16 ? 14 min, NS 27 -C 16 min, P < 0.05). The quality of the sensory block after injection of the local anesthetic was also significantly better in Group US compared with Group NS (US 15% -C 10% of initial value, NS 27% + 14% of initial value, P < 0.05). A good analgesic effect was achieved in 95% of the patients in the US group and in 85% of the patients in the NS group. In the US group, visualization of the cannula tip, the femoral nerve, the major vessels, and the local anesthetic spread was possible in 85% of patients. Incidental arterial puncture (n = 3) was observed only in the NS group. We conclude that an USguided approach for 3-in-1 block reduces the onset time, improves the quality of the sensory block and minimizes the risks associated with this regional anesthetic technique. Implications: The onset time and the quality of a regional anesthetic technique for the lower extremity is improved by ultrasonographic nerve identification compared with older techniques. (Anesth Analg 1997;85:854-7) he 3-in-1 block, including the femoral nerve, the obturator nerve, and the lateral cutaneous femoral nerve, is a widely used regional anesthetic technique for many operations and for pain management of the lower extremity. Indications for the 3-in-1 block include operations and wound care of the thigh, muscle biopsy of the thigh, obturator paralysis for transurethral resection of the prostate and urinary bladder, and, in combination with the sciatic nerve block, all operations for one lower limb. For analgesic therapy, the 3-in-1 block can be used for hip fractures, stump pain after amputation of lower limb, and mobilization therapy of the knee and hip joint.

Coupling of cell-binding ligands to polyethylenimine for targeted gene delivery

Abstract: Recently the high transfection potential of the cationic polymer polyethylenimine (PEI) was described (Boussif O et al. Proc Natl Acad Sci USA 1995; 92: 7297-7301). To combine the promising DNA delivering activity of PEI with the concept of receptor-mediated gene delivery, cell-binding ligands (transferrin or antiCD3 antibody) were incorporated by covalent linkage to PEI. DNA complexes of PEI or ligand-PEI conjugates were tested for transfection of cultured neuroblastoma Neuro 2A cells, melanoma B16 or H225 cells, erythroid leukemic K562 cells and T cell leukemia Jurkat E6.1 cells. Depending on the cell line, incorporation of the cell-binding ligand resulted in an up to 1000-fold increased transfection efficiency. This activity depends on ligand-receptor interaction and was observed also at low PEI cation:DNA anion ratios where ligand-free PEI lacks efficiency. Depending on the cell-binding ligand, specific targeting (CD3 antibody, Jurkat cells) can be achieved. Gene transfer can be augmented by the addition of an endosome-destabilizing influenza peptide, but is not dependent on the presence of additional endosomolytic agents. Application of transferrin-PEI for the production of murine interleukin-2 in B16 cells resulted in exceptionally high secretion rates of 19 micrograms IL-2 protein per 10(6) cells per 24 h.

ProbView: a flexible probabilistic database system

Abstract: Probability theory is mathematically the best understood paradigm for modeling and manipulating uncertain information. Probabilities of complex events can be computed from those of basic events on which they depend, using any of a number of strategies. Which strategy is appropriate depends very much on the known interdependencies among the events involved. Previous work on probabilistic databases has assumed a fixed and restrictivecombination strategy (e.g., assuming all events are pairwise independent). In this article, we characterize, using postulates, whole classes of strategies for conjunction, disjunction, and negation, meaningful from the viewpoint of probability theory. (1) We propose a probabilistic relational data model and a genericprobabilistic relational algebra that neatly captures various strategiessatisfying the postulates, within a single unified framework. (2) We show that as long as the chosen strategies can be computed in polynomial time, queries in the positive fragment of the probabilistic relational algebra have essentially the same data complexity as classical relational algebra. (3) We establish various containments and equivalences between algebraic expressions, similar in spirit to those in classical algebra. (4) We develop algorithms for maintaining materialized probabilistic views. (5) Based on these ideas, we have developed a prototype probabilistic database system called ProbView on top of Dbase V.0. We validate our complexity results with experiments and show that rewriting certain types of queries to other equivalent forms often yields substantial savings.

Immunological changes during specific immunotherapy of grass pollen allergy: reduced lymphoproliferative responses to allergen and shift from TH2 to TH1 in T-cell clones specific for Phl p 1, a major grass pollen allergen.

Abstract: Summary Background and Objective The mechanisms operative in specific immunotherapy (SIT) of Type I allergy are not completely understood. In the present study we evaluated immunological changes during SIT in pollinosis. Method Eight patients suffering from pollinosis (monosensitized to grass pollen) were treated with conventional SIT. All subjects had IgE specific for Phi p 1. a major allergen of timothy grass. In vitro changes in the immunological reactivity to grass pollen extract and to recombinant Phi p 1 were evaluated. Subjects were examined at three occasions: before, after 3 months and after I year of SIT. Results Serological analysis revealed a marked increase of grass pollen- and Phi p 1-specific IgG, titres of specific IgE did not change significantly. Lymphoproliferative responses to grass pollen extract and rPhl p 1 were reduced already after 3 months of treatment. Accordingly, the cloning efficiency for Ph1 p 1-specific T-cell clones (TCC) dropped markedly in all patients. The majority of allergen-specific TCC raised before SIT revealed a TH2-like pattern of cytokine production. TCC established after SIT revealed TH1 characteristics. This shift was due to a decrease in IL-4 rather than an increase in IFN-production by T cells. Investigations of the epitopes recognized by T cells before and after SIT did not reveal the outgrowth of new (ldquo;protecting”) specificities. We could not observe induction of allergen-speeific CD8+ lymphocytes (supressor cells). Conclusion Our data indicate that — on the level of TH lymphocytes — SIT induces tolerance to the allergen and a modulation of the cytokine pattern produced in response to allergen stimulation.

Cancer Mortality in Workers Exposed to Phenoxy Herbicides, Chlorophenols, and Dioxins An Expanded and Updated International Cohort Study

Abstract: The authors examined cancer mortality in a historical cohort study of 21,863 male and female workers in 36 cohorts exposed to phenoxy herbicides, chlorophenols, and dioxins in 12 countries. Subjects in this updated and expanded multinational study coordinated by the International Agency for Research on Cancer were followed from 1939 to 1992. Exposure was reconstructed using job records, company exposure questionnaires, and serum and adipose tissue dioxin levels. Among workers exposed to phenoxy herbicides contaminated with 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) or higher chlorinated dioxins, mortality from soft-tissue sarcoma (6 deaths; standardized mortality ratio (SMR) = 2.03, 95% confidence interval (CI) 0.75-4.43) was higher than expected from national mortality rates. Mortality from all malignant neoplasms (710 deaths; SMR = 1.12, 95% CI 1.04-1.21), non-Hodgkin's lymphoma (24 deaths; SMR = 1.39, 95% CI 0.89-2.06), and lung cancer (225 deaths; SMR = 1.12, 95% CI 0.98-1.28) was slightly elevated. Risks for all neoplasms, for sarcomas, and for lymphomas increased with time since first exposure. In workers exposed to phenoxy herbicides with minimal or no contamination by TCDD and higher chlorinated dioxins, mortality from all neoplasms (398 deaths; SMR = 0.96, 95% CI 0.87-1.06), non-Hodgkin's lymphoma (9 deaths; SMR = 1.00), and lung cancer (148 deaths; SMR = 1.03) was similar to that expected, and mortality from soft-tissue sarcoma was slightly elevated (2 deaths; SMR = 1.35). In a Poisson regression analysis, workers exposed to TCDD or higher chlorinated dioxins had an increased risk for all neoplasms (rate ratio = 1.29, 95% CI 0.94-1.76) compared with workers from the same cohort exposed to phenoxy herbicides and chlorophenols but with minimal or no exposure to TCDD and higher chlorinated dioxins. These findings indicate that exposure to herbicides contaminated with TCDD and higher chlorinated dioxins may be associated with a small increase in overall cancer risk and in risk for specific cancers.

Wavelength-tuning interferometry of intraocular distances.

Abstract: We describe basic principles of wavelength-tuning interferometry and demonstrate its application in ophthalmology. The advantage of this technique compared with conventional low-coherence interferometry ranging is the simultaneous measurement of the object structure without the need for a moving reference mirror. Shifting the wavelength of an external-cavity tunable laser diode causes intensity oscillations in the interference pattern of light beams remitted from the intraocular structure. A Fourier transform of the corresponding wave-number-dependent photodetector signal yields the distribution of the scattering potential along the light beam illuminating the eye. We use an external interferometer to linearize the wave-number axis. We obtain high resolution in a model eye by slow tuning over a wide wavelength range. With lower resolution we demonstrate the simultaneous measurement of anterior segment length, vitreous chamber depth, and axial eye length in human eyes in vivo with data-acquisition times in the millisecond range.

Variable platelet response to low-dose ASA and the risk of limb deterioration in patients submitted to peripheral arterial angioplasty.

Abstract: A group of 100 patients with intermittent claudication (70 male, 30 female), treated with I00 mg ASA per day, were followed over 18 months after elective percutaneous balloon angioplasty. Platelet function was monitored over a period of 12 months by corrected whole blood aggregometry (CWBA). Upon stimulation by arachidonic acid (AA), adenosine diphosphate (ADP) and collagen, CWBA-results were obtained by an electronic acquisition and evaluation system correcting for hematocrit and platelet count of the blood sample. All patients showed a completely inhibited platelet response to AA stimulation. Comparison of the CWBA-results with clinical parameters revealed that reocclusions at the site of angioplasty occurred exclusively in male patients for which CWBA failed to prove an inhibition of aggregation upon both agonists, ADP and collagen, and for these patients the risk of complication is at least 87% higher (p = 0.0093). Only 40% of male patients show the expected effect of ASA on in vitro platelet aggregation at any given point in time and CWBA is capable of predicting those male patients which are at an elevated risk of reocclusion following peripheral angioplasty.

Single-Bond Torsional Potentials in Conjugated Systems: A Comparison of ab Initio and Density Functional Results

Abstract: The fully relaxed single-bond torsional potentials in typical conjugated systems were evaluated with the aid of ab initio self-consistent-field and Moller−Plesset second-order calculations and, additionally, with several recently developed variants of the density functional theory. For this systematic investigation, 1,3-butadiene, styrene, biphenyl, 2,2‘-bithiophene, 2,2‘-bipyrrole and 2,2‘-bifuran have been selected as model molecules. As representative examples for nonconjugated systems, the molecules n-butane and 1-butene have been treated at the very same calculational levels. For all conjugated molecules, the electron correlation corrections to the self-consistent-field torsional potentials, as obtained with the density functional methods, are dramatically different from those resulting from the more conventional Moller−Plesset second-order approximation. For those cases where experimental data for torsional barriers are available, the self-consistent-field and the Moller−Plesset second-order results...

The photo-fenton oxidation : a cheap and efficient wastewater treatment method

Abstract: Advanced Oxidation Processes (AOPs) for wastewater treatment are gaining more importance since biological treatment plants are often not sufficient for highly contaminated or toxic wastewaters. In order to find out the most efficient and cheap AOP, investigations were concentrated on methods that can use sunlight. The systems TiO2/UV, Fe2+/H2O2/UV (Photo-Fenton reaction), Fe2+/O2/UV and Fe2+/O3/UV were compared. Since the Photo-Fenton system was the most effective, pilot plant experiments with industrial wastewaters and sunlight experiments were carried out. Finally a rough cost estimate shows that Photo-Fenton treatment with sunlight is far cheaper than other available AOPs, namely ozonization.

l-Arginine Treatment Alters the Kinetics of Nitric Oxide and Superoxide Release and Reduces Ischemia/Reperfusion Injury in Skeletal Muscle

Abstract: Background Constitutive nitric oxide synthase (cNOS) may produce species involved in ischemia/reperfusion (I/R) injury: NO in the presence of sufficient l-arginine and superoxide at the diminished local l-arginine concentration accompanying I/R. Methods and Results During hindlimb I/R (2.5 hours/2 hours), in vivo NO was continuously monitored (porphyrinic sensor), and l-arginine (chromatography), superoxide (chemiluminescence), and I/R injury were measured intermittently. Normal rabbits were compared with those infused with l-arginine 4 mg·kg−1·min−1 for 1 hour. In both groups, ≈6 minutes into ischemia, a rapid increase of NO from its basal level of 50±17 to 115±7 nmol/L, P<.005 (microvessels), was observed. In animals not treated with l-arginine, NO dropped below basal to undetectable levels (<1 nmol/L) during reperfusion. In animals treated with l-arginine, the decrease of NO was slower, such that substantial amounts accumulated during reperfusion (25 nmol/L). Decreased NO during I/R was accompanied by ...

Genistein, the Dietary-Derived Angiogenesis Inhibitor, Prevents LDL Oxidation and Protects Endothelial Cells From Damage by Atherogenic LDL

Abstract: There is now growing evidence that the oxidative modification of LDL plays a potential role in atherosclerosis. In this study, genistein, a compound derived from a soy diet with a flavonoid chemical structure (4',5,7-trihydroxyisoflavone), which was found to inhibit angiogenesis, has been evaluated for its ability to act as an LDL antioxidant and a vascular cell protective agent against oxidized LDL. The results showed that genistein was able to inhibit the oxidation of LDL in the presence of copper ions or superoxide/nitric oxide radicals as measured by thiobarbituric acid-reactive substance formation, alteration in electrophoretic mobility, and lipid hydroperoxides. Bovine aortic endothelial cell- and human endothelial cell-mediated LDL oxidation was also inhibited in the presence of genistein. The 7-O-glucoside of genistein, genistin, was much less effective in inhibiting LDL oxidation in the cell-free and cell-mediated lipoprotein-oxidating systems. Incubating human endothelial cells in the absence or presence of genistein and challenging the cells with already oxidized lipoprotein revealed that in addition to its antioxidative potential during LDL oxidating processes, genistein effectively protected the vascular cells from damage by oxidized lipoproteins. The tyrosine kinase inhibitor genistein was found to block upregulation of two tyrosine-phosphorylated proteins of 132 and 69 kDa in endothelial cells induced by oxidized LDL. Parallel experiments with the inactive analogue daidzein, however, showed that the cytoprotective effect of the isoflavones seems not to be dependent on tyrosine phosphorylation. Our findings will support the suggested and documented beneficial action of a soy diet in preventing chronic vascular diseases and early atherogenic events.