Institution
University of Vienna
Education•Vienna, Austria•
About: University of Vienna is a education organization based out in Vienna, Austria. It is known for research contribution in the topics: Population & Stars. The organization has 44686 authors who have published 95840 publications receiving 2907492 citations.
Topics: Population, Stars, Galaxy, Transplantation, Crystal structure
Papers published on a yearly basis
Papers
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TL;DR: Application of the small particles in more concentrated form and over extended periods of time improves transfection activity, and analysis of gene expression at the cellular level revealed that the differences in overall gene expression largely result from different intensities per expressing cell, while the difference in the percentage of expressing cells is less substantial.
Abstract: Under physiological salt concentration, plasmid DNA complexed with transferrin-conjugated or unmodified polyethylenimine (PEI, 800 kDa) forms huge (up to > 1000 nm) aggregates, unless the individual components are mixed at a highly positive nitrogen/phosphate (N/P) charge ratio. At low ionic strengths, however, small particles with an average size of 40 nm are formed over a broad range of N/P ratios. Interestingly, in transfection experiments these small particles result in a 10-fold (B16F10 cells) to more than 100-fold (Neuro2A cells, K562 cells) reduced luciferase gene expression efficiency in comparison to the large complexes formed in physiological salt solutions. Limited transport of the small particles to the cell surfaces is one possible reason for this effect. Application of the small particles in more concentrated form and over extended periods of time improves transfection activity. Reduced intracellular release may be another explanation for the decreased transfection efficiency; incubation with chloroquine or incorporation of the endosomolytic peptide INF5 into the small complexes enhances gene expression approximately 10-fold. Analysis of gene expression at the cellular level using a green fluorescence protein reporter gene and flow cytometry revealed that the differences in overall gene expression largely result from different intensities per expressing cell, while the difference in the percentage of expressing cells is less substantial.
594 citations
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Bielefeld University1, BRICS2, University of Düsseldorf3, Oregon State University4, University of California, San Diego5, Aarhus University6, University of Copenhagen7, Roskilde University8, Joint Genome Institute9, Pittsburgh Supercomputing Center10, Saint Petersburg State University11, Max Planck Society12, University of Vienna13, University of Technology, Sydney14, Centre national de la recherche scientifique15, Genome Institute of Singapore16, University of Warwick17, University of Tübingen18, Intel19, French Institute for Research in Computer Science and Automation20, Taipei Medical University21, Joint BioEnergy Institute22, Lawrence Berkeley National Laboratory23, Georgia Institute of Technology24, University of Calgary25, University of Göttingen26, National Health Research Institutes27, San Diego State University28, Boyce Thompson Institute for Plant Research29, Coordenadoria de Aperfeiçoamento de Pessoal de Nível Superior30, Robert Koch Institute31, University of Maryland, College Park32, Newcastle University33, Leibniz Association34, ETH Zurich35
TL;DR: The Critical Assessment of Metagenome Interpretation (CAMI) challenge has engaged the global developer community to benchmark their programs on highly complex and realistic data sets, generated from ∼700 newly sequenced microorganisms and ∼600 novel viruses and plasmids and representing common experimental setups as discussed by the authors.
Abstract: Methods for assembly, taxonomic profiling and binning are key to interpreting metagenome data, but a lack of consensus about benchmarking complicates performance assessment. The Critical Assessment of Metagenome Interpretation (CAMI) challenge has engaged the global developer community to benchmark their programs on highly complex and realistic data sets, generated from ∼700 newly sequenced microorganisms and ∼600 novel viruses and plasmids and representing common experimental setups. Assembly and genome binning programs performed well for species represented by individual genomes but were substantially affected by the presence of related strains. Taxonomic profiling and binning programs were proficient at high taxonomic ranks, with a notable performance decrease below family level. Parameter settings markedly affected performance, underscoring their importance for program reproducibility. The CAMI results highlight current challenges but also provide a roadmap for software selection to answer specific research questions.
593 citations
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TL;DR: ROTEM®-guided haemostatic therapy, with fibrinogen concentrate as first-line haemOSTatic therapy and additional PCC, was goal-directed and fast, and a favourable survival rate was observed.
Abstract: The appropriate strategy for trauma-induced coagulopathy management is under debate. We report the treatment of major trauma using mainly coagulation factor concentrates. This retrospective analysis included trauma patients who received ≥ 5 units of red blood cell concentrate within 24 hours. Coagulation management was guided by thromboelastometry (ROTEM®). Fibrinogen concentrate was given as first-line haemostatic therapy when maximum clot firmness (MCF) measured by FibTEM (fibrin-based test) was 1.5 times normal. Lack of improvement in EXTEM MCF after fibrinogen concentrate administration was an indication for platelet concentrate. The observed mortality was compared with the mortality predicted by the trauma injury severity score (TRISS) and by the revised injury severity classification (RISC) score. Of 131 patients included, 128 received fibrinogen concentrate as first-line therapy, 98 additionally received PCC, while 3 patients with recent coumarin intake received only PCC. Twelve patients received FFP and 29 received platelet concentrate. The observed mortality was 24.4%, lower than the TRISS mortality of 33.7% (P = 0.032) and the RISC mortality of 28.7% (P > 0.05). After excluding 17 patients with traumatic brain injury, the difference in mortality was 14% observed versus 27.8% predicted by TRISS (P = 0.0018) and 24.3% predicted by RISC (P = 0.014). ROTEM®-guided haemostatic therapy, with fibrinogen concentrate as first-line haemostatic therapy and additional PCC, was goal-directed and fast. A favourable survival rate was observed. Prospective, randomized trials to investigate this therapeutic alternative further appear warranted.
591 citations
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TL;DR: In this paper, the authors provide systematic research-based evidence on the usage of the BSC concept in German-speaking countries and develop three primary types of BSC usage reflecting the successive phases in the evolution of the balanced scorecard concept and of its implementation in practice.
Abstract: This paper provides systematic research-based evidence on the usage of the BSC concept in German-speaking countries. We develop three primary types of BSC usage reflecting the successive phases in the evolution of the BSC concept and of its implementation in practice. This typology ranges from the BSC's origin as a multidimensional framework for strategic performance measurement that combines financial and non-financial measures to its advanced usage as an integrated strategic management system that describes strategy by a cause-and-effect logic and that is linked to the reward system. We use this theoretical framework to analyze the spread, implementation and benefits of the different types of Balanced Scorecards. Moreover, the impact of size and industry on BSC usage is analyzed. Our sample consists of a clearly defined segment of the most important publicly traded firms in Germany, Austria and Switzerland and we achieved an overall response rate of 87%.
587 citations
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TL;DR: The experimental confirmation by demonstrating a violation of a generalized Clauser-Horne-Shimony-Holt-type Bell inequality in three dimensions by more than 18 standard deviations is reported.
Abstract: Orbital angular momentum entangled photons emitted by a down-conversion source are in higher dimensional entangled states. Here we report the experimental confirmation by demonstrating a violation of a generalized Clauser-Horne-Shimony-Holt-type Bell inequality in three dimensions by more than 18 standard deviations. Higher dimensional entangled states allow the realization of new types of quantum communication protocols. They also provide a more secure quantum cryptography scheme. Therefore our experimental results are likely to have applications in future quantum communication technology.
587 citations
Authors
Showing all 45262 results
Name | H-index | Papers | Citations |
---|---|---|---|
Tomas Hökfelt | 158 | 1033 | 95979 |
Wolfgang Wagner | 156 | 2342 | 123391 |
Hans Lassmann | 155 | 724 | 79933 |
Stanley J. Korsmeyer | 151 | 316 | 113691 |
Charles B. Nemeroff | 149 | 979 | 90426 |
Martin A. Nowak | 148 | 591 | 94394 |
Barton F. Haynes | 144 | 911 | 79014 |
Yi Yang | 143 | 2456 | 92268 |
Peter Palese | 132 | 526 | 57882 |
Gérald Simonneau | 130 | 587 | 90006 |
Peter M. Elias | 127 | 581 | 49825 |
Erwin F. Wagner | 125 | 375 | 59688 |
Anton Zeilinger | 125 | 631 | 71013 |
Wolfgang Waltenberger | 125 | 854 | 75841 |
Michael Wagner | 124 | 351 | 54251 |