University of Virginia

About: University of Virginia is a education organization based out in Charlottesville, Virginia, United States. It is known for research contribution in the topics: Population & Poison control. The organization has 52543 authors who have published 113268 publications receiving 5220506 citations. The organization is also known as: U of V & UVa.

A large nucleolar U3 ribonucleoprotein required for 18S ribosomal RNA biogenesis

Abstract: Although the U3 small nucleolar RNA (snoRNA), a member of the box C/D class of snoRNAs, was identified with the spliceosomal small nuclear RNAs (snRNAs) over 30 years ago, its function and its associated protein components have remained more elusive. The U3 snoRNA is ubiquitous in eukaryotes and is required for nucleolar processing of pre-18S ribosomal RNA in all organisms where it has been tested. Biochemical and genetic analyses suggest that U3 pre-rRNA base-pairing interactions mediate endonucleolytic pre-rRNA cleavages. Here we have purified a large ribonucleoprotein (RNP) complex from Saccharomyces cerevisiae that contains the U3 snoRNA and 28 proteins. Seventeen new proteins (Utp1 17) and Rrp5 were present, as were ten known components. The Utp proteins are nucleolar and specifically associated with the U3 snoRNA. Depletion of the Utp proteins impedes production of the 18S rRNA, indicating that they are part of the active pre-rRNA processing complex. On the basis of its large size (80S; calculated relative molecular mass of at least 2,200,000) and function, this complex may correspond to the terminal knobs present at the 5' ends of nascent pre-rRNAs. We have termed this large RNP the small subunit (SSU) processome.

Focal adhesion kinase and paxillin bind to peptides mimicking beta integrin cytoplasmic domains.

Abstract: The integrins have recently been implicated in signal transduction. A likely mediator of integrin signaling is focal adhesion kinase (pp125FAK or FAK), a structurally distinct protein tyrosine kinase that becomes enzymatically activated upon engagement of integrins with their ligands. A second candidate signaling molecule is paxillin, a focal adhesion associated, cytoskeletal protein that coordinately becomes phosphorylated on tyrosine upon activation of pp125FAK. Paxillin physically complexes with two protein tyrosine kinases, pp60src and Csk (COOH-terminal src kinase), and the oncoprotein p47gag-crk, each of which could function as part of a paxillin signaling complex. Using an in vitro assay we have established that the cytoplasmic domain of the beta 1 integrin can bind to paxillin and pp125FAK from chicken embryo cell lysates. The NH2-terminal, noncatalytic domain of pp125FAK can bind directly to the cytoplasmic tail of beta 1 and recognizes integrin sequences distinct from those involved in binding to alpha-actinin. Paxillin binding is independent of pp125FAK binding despite the fact that both bind to the same region of beta 1. These results demonstrate that the cytoplasmic domain of the beta subunits of integrins contain binding sites for both signaling molecules and structural proteins suggesting that integrins can coordinate the generation of cytoplasmic signals in addition to their role in anchoring components of the cytoskeleton.

Assessment as Learning: Using Classroom Assessment to Maximize Student Learning

Abstract: Este titulo proporcionara a los maestros y la escuela y los administradores escolares un entendimiento de las razones detras de su confusion e incomodidad al detallar la forma en que el papel cambiante de la educacion y nuestro creciente conocimiento sobre la naturaleza del aprendizaje han hecho que la evaluacion en el aula sea mucho mas compleja.

Dead Or Alive? The Development, Trajectory And Future Of Technology Adoption Research.

Abstract: Research on individual-level technology adoption is one of the most mature streams of information systems (IS) research. In this paper, we compare the progress in the area of technology adoption with two widely-researched streams in psychology and organizational behavior: theory of planned behavior and job satisfaction. In addition to gauging the progress in technology adoption research, this allows us to identify some fruitful areas for future research. Based on our comparison, we conclude that there has been excellent progress in technology adoption research. However, as a next step, we call for research focused on interventions, contingencies, and alternative theoretical perspectives (to the largely social psychologybased technology adoption research. Also, we believe it would be important to use the comparisons discussed here as a basis to develop a framework-driven set of future research directions to guide further work in this area.