University of Warwick

About: University of Warwick is a education organization based out in Coventry, Warwickshire, United Kingdom. It is known for research contribution in the topics: Population & Context (language use). The organization has 26212 authors who have published 77127 publications receiving 2666552 citations. The organization is also known as: Warwick University & The University of Warwick.

The concordance between RNA-seq and microarray data depends on chemical treatment and transcript abundance

Abstract: The concordance of RNA-sequencing (RNA-seq) with microarrays for genome-wide analysis of differential gene expression has not been rigorously assessed using a range of chemical treatment conditions. Here we use a comprehensive study design to generate Illumina RNA-seq and Affymetrix microarray data from the same liver samples of rats exposed in triplicate to varying degrees of perturbation by 27 chemicals representing multiple modes of action (MOAs). The cross-platform concordance in terms of differentially expressed genes (DEGs) or enriched pathways is linearly correlated with treatment effect size (R(2)0.8). Furthermore, the concordance is also affected by transcript abundance and biological complexity of the MOA. RNA-seq outperforms microarray (93% versus 75%) in DEG verification as assessed by quantitative PCR, with the gain mainly due to its improved accuracy for low-abundance transcripts. Nonetheless, classifiers to predict MOAs perform similarly when developed using data from either platform. Therefore, the endpoint studied and its biological complexity, transcript abundance and the genomic application are important factors in transcriptomic research and for clinical and regulatory decision making.

Coexistence of Wi-Fi and heterogeneous small cell networks sharing unlicensed spectrum

Abstract: As two major players in terrestrial wireless communications, Wi-Fi systems and cellular networks have different origins and have largely evolved separately. Motivated by the exponentially increasing wireless data demand, cellular networks are evolving towards a heterogeneous and small cell network architecture, wherein small cells are expected to provide very high capacity. However, due to the limited licensed spectrum for cellular networks, any effort to achieve capacity growth through network densification will face the challenge of severe inter-cell interference. In view of this, recent standardization developments have started to consider the opportunities for cellular networks to use the unlicensed spectrum bands, including the 2.4 GHz and 5 GHz bands that are currently used by Wi-Fi, Zigbee and some other communication systems. In this article, we look into the coexistence of Wi-Fi and 4G cellular networks sharing the unlicensed spectrum. We introduce a network architecture where small cells use the same unlicensed spectrum that Wi-Fi systems operate in without affecting the performance of Wi-Fi systems. We present an almost blank subframe (ABS) scheme without priority to mitigate the co-channel interference from small cells to Wi-Fi systems, and propose an interference avoidance scheme based on small cells estimating the density of nearby Wi-Fi access points to facilitate their coexistence while sharing the same unlicensed spectrum. Simulation results show that the proposed network architecture and interference avoidance schemes can significantly increase the capacity of 4G heterogeneous cellular networks while maintaining the service quality of Wi-Fi systems.