Showing papers by "University of Western Australia published in 1998"

Hypovitaminosis D in Medical Inpatients

Abstract: Background Vitamin D deficiency is a major risk factor for bone loss and fracture. Although hypovitaminosis D has been detected frequently in elderly and housebound people, the prevalence of vitamin D deficiency among patients hospitalized on a general medical service is unknown. Methods We assessed vitamin D intake, ultraviolet-light exposure, and risk factors for hypovitaminosis D and measured serum 25-hydroxyvitamin D, parathyroid hormone, and ionized calcium in 290 consecutive patients on a general medical ward. Results A total of 164 patients (57 percent) were considered vitamin D–deficient (serum concentration of 25-hydroxyvitamin D, <15 ng per milliliter), of whom 65 (22 percent) were considered severely vitamin D–deficient (serum concentration of 25-hydroxyvitamin D, <8 ng per milliliter). Serum 25-hydroxyvitamin D concentrations were related inversely to parathyroid hormone concentrations. Lower vitamin D intake, less exposure to ultraviolet light, anticonvulsant-drug therapy, renal dialysis, nep...

Effects of sexual dimorphism on facial attractiveness

Abstract: Testosterone-dependent secondary sexual characteristics in males may signal immunological competence1 and are sexually selected for in several species2,3. In humans, oestrogen-dependent characteristics of the female body correlate with health and reproductive fitness and are found attractive4,5,6. Enhancing the sexual dimorphism of human faces should raise attractiveness by enhancing sex-hormone-related cues to youth and fertility in females5,7,8,9,10,11, and to dominance and immunocompetence in males5,12,13. Here we report the results of asking subjects to choose the most attractive faces from continua that enhanced or diminished differences between the average shape of female and male faces. As predicted, subjects preferred feminized to average shapes of a female face. This preference applied across UK and Japanese populations but was stronger for within-population judgements, which indicates that attractiveness cues are learned. Subjects preferred feminized to average or masculinized shapes of a male face. Enhancing masculine facial characteristics increased both perceived dominance and negative attributions (for example, coldness or dishonesty) relevant to relationships and paternal investment. These results indicate a selection pressure that limits sexual dimorphism and encourages neoteny in humans.

Extending the simple linear regression model to account for correlated responses: an introduction to generalized estimating equations and multi-level mixed modelling.

Abstract: Much of the research in epidemiology and clinical science is based upon longitudinal designs which involve repeated measurements of a variable of interest in each of a series of individuals. Such designs can be very powerful, both statistically and scientifically, because they enable one to study changes within individual subjects over time or under varied conditions. However, this power arises because the repeated measurements tend to be correlated with one another, and this must be taken into proper account at the time of analysis or misleading conclusions may result. Recent advances in statistical theory and in software development mean that studies based upon such designs can now be analysed more easily, in a valid yet flexible manner, using a variety of approaches which include the use of generalized estimating equations, and mixed models which incorporate random effects. This paper provides a particularly simple illustration of the use of these two approaches, taking as a practical example the analysis of a study which examined the response of portable peak expiratory flow meters to changes in true peak expiratory flow in 12 children with asthma. The paper takes the reader through the relevant practicalities of model fitting, interpretation and criticism and demonstrates that, in a simple case such as this, analyses based upon these model-based approaches produce reassuringly similar inferences to standard analyses based upon more conventional methods.

The redistribution of soil water by tree root systems.

Abstract: Plant roots transfer water between soil layers of different water potential thereby significantly affecting the distribution and availability of water in the soil profile We used a modification of the heat pulse method to measure sap flow in roots of Grevillea robusta and Eucalyptus camaldulensis and demonstrated a redistribution of soil water from deeper in the profile to dry surface horizons by the root system This phenomenon, termed “hydraulic lift” has been reported previously However, we also demonstrated that after the surface soils were rewetted at the break of season, water was transported by roots from the surface to deeper soil horizons – the reverse of the “hydraulic lift” behaviour described for other woody species We suggest that “hydraulic redistribution” of water in tree roots is significant in maintaining root viability, facilitating root growth in dry soils and modifying resource availability

Facial symmetry and the perception of Beauty

Abstract: Evolutionary, as well as cultural, pressures may contribute to our perceptions of facial attractiveness. Biologists predict that facial symmetry should be attractive, because it may signal mate quality. We tested the prediction that facial symmetry is attractive by manipulating the symmetry of individual faces and observing the effect on attractiveness, and by examining whether natural variations in symmetry (between faces) correlated with perceived attractiveness. Attractiveness increased when we increased symmetry, and decreased when we reduced symmetry, in individual faces (Experiment 1), and natural variations in symmetry correlated significantly with attractiveness (Experiments 1 and 1A). Perfectly symmetric versions, made by blending the normal and mirror images of each face, were preferred to less symmetric versions of the same faces (even when those versions were also blends) (Experiments 1 and 2). Similar results were found when subjects judged the faces on appeal as a potential life partner, suggesting that facial symmetry may affect human mate choice. We conclude that facial symmetry is attractive and discuss the possibility that this preference for symmetry may be biologically based.

The Chemistry and Biological Effects of Flavonoids and Phenolic Acidsa

Abstract: Flavonoids and phenolic acids are widely distributed in higher plants and form part of the human diet. Recent interest in these substances has been stimulated by the potential health benefits arising from the antioxidant activity of these polyphenolic compounds. This review outlines the basic chemistry, biosynthesis, and structure-activity relationships of these compounds with respect to their antioxidant activity. Although there is considerable in vitro evidence establishing antioxidant activity for polyphenolics found in the diet, there are few studies in humans on the absorption and bioavailability of these compounds. The possible in vivo antioxidant effects of the flavonoids is even less well understood. For example, controlled human intervention studies with beverages, such as red wine, that are rich in polyphenolic compounds, have yielded conflicting results. Our own work and that of others suggests that the final effects of such beverages may be a balance between the well-described prooxidant effects of alcohol and its metabolism and the antioxidant effects of the polyphenolic constituents. There is a need for further studies to increase our understanding of the absorption and in vivo biological effects of this family of compounds.

Strong coupling phenomena in quantum microcavity structures

Abstract: The physics of strong coupling phenomena in semiconductor quantum microcavities is reviewed This is a relatively new field with most important developments having occurred in the last 5 years We describe how such microcavities enable both electronic and photonic properties of semiconductors, and the interaction between them, to be controlled in the same structure The resulting coupled exciton-photon eigenstates, cavity polaritons, have many interesting properties including very low mass for small in-plane wavevectors, and can be studied easily and directly in optical experiments, unlike exciton-polaritons in bulk semiconductors A wealth of new optical phenomena has been reported in this field in the last few years This review describes the most important of these phenomena We discuss the reasons why polaritons have fundamentally different properties in microcavities as compared with those in bulk materials or quantum wells We explain the factors which control the strength of the exciton-photon coupling and how the resulting optical spectra can be modelled We then emphasize, in the main body of the review, the particularly important results of reflectivity experiments at both normal and oblique angles of incidence, the effects of external electric and magnetic fields, the results of coherent Raman scattering experiments, the effects of disorder on microcavity spectra, including the observation of motional narrowing over the exciton disorder potential, studies of coupled microcavities, and photoluminescence and time-resolved phenomena

Oval Cell Numbers in Human Chronic Liver Diseases are directly related to Disease Severity

Abstract: The risk of developing hepatocellular carcinoma is significantly increased in patients with genetic hemochromatosis, alcoholic liver disease, or chronic hepatitis C infection. The precise mechanisms underlying the development of hepatocellular carcinoma in these conditions are not well understood. Stem cells within the liver, termed oval cells, are involved in the pathogenesis of hepatocellular carcinoma in animal models and may be important in the development of hepatocellular carcinoma in human chronic liver diseases. The aims of this study were to determine whether oval cells could be detected in the liver of patients with genetic hemochromatosis, alcoholic liver disease, or chronic hepatitis C, and whether there is a relationship between the severity of the liver disease and the number of oval cells. Oval cells were detected using histology and immunohistochemistry in liver biopsies from patients with genetic hemochromatosis, alcoholic liver disease, or chronic hepatitis C. Oval cells were not observed in normal liver controls. Oval cell numbers increased significantly with the progression of disease severity from mild to severe in each of the diseases studied. We conclude that oval cells are frequently found in subjects with genetic hemochromatosis, alcoholic liver disease, or chronic hepatitis C. There is an association between severity of liver disease and increase in the number of oval cells consistent with the hypothesis that oval cell proliferation is associated with increased risk for development of hepatocellular carcinoma in chronic liver disease.

Consumer Perceived Value: Development of a Multiple Item Scale

Abstract: Abstract Value creation is widely discussed in the practitioner literature and is often a part of organizations’ mission statements and objectives. It is seen by many commentators as the key to long-term success, with Albrecht (1992 , p 7) arguing that “the only thing that matters in the new world of quality is delivering customer value.” Despite this emphasis, little research has addressed the value construct itself and there is no well-accepted value measure, even in the retail environment in which customers evaluate products before purchase. The present research project describes the development of a 19-item measure, PERVAL, that can be used to assess customers’ perceptions of the value of a consumer durable good at a brand level. The measure was developed for use in a retail purchase situation to determine what consumption values drive purchase attitude and behavior. Four distinct, value dimensions emerged that were termed emotional, social, quality/performance and price/value for money. The reliability and validity of the scale was assessed in a prepurchase situation, using exploratory and confirmatory analyses. All four value dimensions were found to help significantly in explaining attitudes and behavior. The scale was also tested in a postpurchase situation and found to be both reliable and valid in this context as well. The PERVAL scale has a variety of potential applications and can serve as a framework for further empirical research in this important area.

10 – Sperm Competition in Insects: Mechanisms and the Potential for Selection

Abstract: The chapter focuses on the understanding of the mechanisms of sperm competition, which are essential for interpreting nonrandom patterns of paternity and for predicting the types of adaptations that sperm competition can generate. P2 values are often used to infer the mechanism of underlying patterns of sperm utilization: intermediate values are taken as indicative of sperm mixing while higher values of P2 are taken as evidence for sperm precedence or sperm displacement. The bimodal distribution of P2 values seen in Lepidoptera is an excellent example of how little the mean value of P2 can tell us about the patterns of sperm competition. Patterns of sperm competition can change markedly with changes in the intervals between copulations. It is clear that males can adjust the numbers of sperm ejaculated into females, depending on female quality and/or the risks of sperm competition. Thus, patterns of sperm utilization depend on the behavior of males. Alternatively, they also depend on the responses of females to males that vary in their quality as mates. Variation in the numbers of sperm inseminated into the female prior to the final mating can have marked effects on the patterns of sperm utilization.

Tissue Hyperplasia and Enhanced T-Cell Signalling via ZAP-70 in c-Cbl-Deficient Mice

Abstract: The c-Cbl protein is tyrosine phosphorylated and forms complexes with a wide range of signalling partners in response to various growth factors. How c-Cbl interacts with proteins, such as Grb2, phosphatidylinositol 3-kinase, and phosphorylated receptors, is well understood, but its role in these complexes is unclear. Recently, the Caenorhabditis elegans Cbl homolog, Sli-1, was shown to act as a negative regulator of epidermal growth factor receptor signalling. This finding forced a reassessment of the role of Cbl proteins and highlighted the desirability of testing genetically whether c-Cbl acts as a negative regulator of mammalian signalling. Here we investigate the role of c-Cbl in development and homeostasis in mice by targeted disruption of the c-Cbl locus. c-Cbl-deficient mice were viable, fertile, and outwardly normal in appearance. Bone development and remodelling also appeared normal in c-Cbl mutants, despite a previously reported requirement for c-Cbl in osteoclast function. However, consistent with a high level of expression of c-Cbl in the hemopoietic compartment, c-Cbl-deficient mice displayed marked changes in their hemopoietic profiles, including altered T-cell receptor expression, lymphoid hyperplasia, and primary splenic extramedullary hemopoiesis. The mammary fat pads of mutant female mice also showed increased ductal density and branching compared to those of their wild-type littermates, indicating an unanticipated role for c-Cbl in regulating mammary growth. Collectively, the hyperplastic histological changes seen in c-Cbl mutant mice are indicative of a normal role for c-Cbl in negatively regulating signalling events that control cell growth. Consistent with this view, we observed greatly increased intracellular protein tyrosine phosphorylation in thymocytes following CD3e cross-linking. In particular, phosphorylation of ZAP-70 kinase in thymocytes was uncoupled from a requirement for CD4-mediated Lck activation. This study provides the first biochemical characterization of any organism that is deficient in a member of this unique protein family. Our findings demonstrate critical roles for c-Cbl in hemopoiesis and in controlling cellular proliferation and signalling by the Syk/ZAP-70 family of protein kinases.

Empirical studies of self-employment

Abstract: This paper presents a review of empirical studies of self-employment for the Australian, Canadian, Dutch, UK and US labour markets. Both cross-sectional and longitudinal studies are analysed. Analyses using cross-sectional data examine the propensity to be self-employed at any one point in time, whereas longitudinal studies focus on the transition into self-employment from wage/salary employment and the survival rate in this state over time. Various hypotheses advanced in the economics and sociology literatures on self-employment are tested. These include the relationship between managerial ability and the propensity to be self-employed and the impact of financial constraints on entry into self-employment stressed in economic models of entrepreneurship, and the relationships between self-employment choice and the nature of the work and group characteristics (e.g., ethnic enclaves) stressed in sociological models of entrepreneurship. The evidence shows that self-employment outcomes are significantly affected by factors such as individual abilities, family background, occupational status, liquidity constraints and ethnic enclaves.

Development of PCR assays for species- and type-specific identification of Pasteurella multocida isolates.

Abstract: Genomic subtractive hybridization of closely related Pasteurella multocida isolates has generated clones useful in distinguishing hemorrhagic septicemia-causing type B strains from other P. multocida serotypes. Oligonucleotide primers designed during the sequencing of these clones have proved valuable in the development of PCR assays for rapid species- and type-specific detection of P. multocida and of type B:2 in particular. This study demonstrated that the primer pair designed from the sequence of the clone 6b (KTT72 and KTSP61) specifically amplified a DNA fragment from types B:2, B:5, and B:2,5 P. multocida and that the primers KMT1T7 and KMT1SP6 produced an amplification product unique to all P. multocida isolates analyzed. It was also shown that PCR amplification performed directly on bacterial colonies or cultures represents an extremely rapid, sensitive method of P. multocida identification.

Dust Mite Proteolytic Allergens Induce Cytokine Release from Cultured Airway Epithelium

Abstract: Endogenous proteolytic enzymes have been shown to be potential sources of airway inflammation inducing proinflammatory cytokine release from respiratory epithelial cells; however, whether any of the exogenous proteases from important allergen sources such as the house dust mite present in our environment behave in a similar fashion is unclear. To this end, we have investigated whether the mite cysteine and serine proteolytic allergens, Der p 1 and Der p 9, respectively, induced cytokine production from primary human bronchial epithelial cells and from the epithelial cell line BEAS-2B. Cells were exposed to mite proteases, and cells or supernatants were assayed for cytokine release, cytokine mRNA expression, and modulation of intracellular calcium ion concentration. Both proteases induced concentration- and time-dependent increases in the release of granulocyte-macrophage (GM)-CSF, IL-6, and IL-8 as well as an increase in the expression of IL-6 mRNA. Cytokine release and mRNA expression were first observed at 8 h and 2 h after protease exposure, respectively. The minimum concentration of each protease that was required to stimulate GM-CSF, IL-6, and IL-8 release was ∼10 ng/ml. Cytokine release was initiated by 1 to 2 h of protease exposure, although maximum concentrations were detected only after a 24-h incubation. IL-6, but not IL-8 and GM-CSF, was shown to be degraded by both proteases at concentrations of >2 μg/ml. The proteases also stimulated changes in the intracellular calcium ion concentration. All mite protease-induced phenomena were inhibited using appropriate protease inhibitors. These results suggest that the proteolytic activity of an allergen may stimulate the release of proinflammatory cytokines from human bronchial epithelium.

A practical numerical approach for large deformation problems in soil

Abstract: A practical method is presented for numerical analysis of problems in solid (in particular soil) mechanics which involve large strains or deformations. The method is similar to what is referred to as ‘arbitrary Lagrangian–Eulerian’, with simple infinitesimal strain incremental analysis combined with regular updating of co-ordinates, remeshing of the domain and interpolation of material and stress parameters. The technique thus differs from the Lagrangian or Eulerian methods more commonly used. Remeshing is accomplished using a fully automatic remeshing technique based on normal offsetting, Delaunay triangulation and Laplacian smoothing. This technique is efficient and robust. It ensures good quality shape and distribution of elements for boundary regions of irregular shape, and is very quick computationally. With remeshing and interpolation, small fluctuations appeared initially in the load-deformation results. In order to minimize these, different increment sizes and remeshing frequencies were explored. Also, various planar linear interpolation techniques were compared, and the unique element method found to work best. Application of the technique is focused on the widespread problem of penetration of surface foundations into soft soil, including deep penetration of foundations where soil flows back over the upper surface of the foundation. Numerical results are presented for a plane strain footing and an axisymmetric jack-up (spudcan) foundation, penetrating deeply into soil which has been modelled as a simple Tresca or Von Mises material, but allowing for increase of the soil strength with depth. The computed results are compared with plasticity solutions for bearing capacity. The numerical method is shown to work extremely well, with potential application to a wide range of soil–structure interaction problems. © 1998 John Wiley & Sons, Ltd.

Small Business Failure and External Risk Factors

Abstract: Unlike much of the previous literature, which has generally focused on internal risk factors, this study seeks to explore the impact of macro-economic factors on small business mortality The results suggest that economic factors appear to be associated with between 30% and 50% of small business failures, depending on the definition of failure used As expected, failure rates were positively associated with interest rates (where failure was defined as bankruptcy) and the rate of unemployment (where failure was defined as discontinuance of ownership) However, somewhat unexpectedly, failure rates were found to be positively associated with lagged employment rates (where failure was defined as to prevent further losses) and with current and lagged retail sales (where failure was defined as either: failed to "make a go of it"; discontinuance of ownership; or discontinuance of business) This indicates that a strengthening economy may provide the trigger for an increase in voluntary business exits as individual proprietors seek to maximize the returns available to them on both their financial and human capital

Age‐associated Changes in the Response of Skeletal Muscle Cells to Exercise and Regenerationa

Abstract: This paper looks at the effects of aging on the response of skeletal muscle to exercise from the perspective of the behavior of muscle precursor cells (widely termed satellite cells or myoblasts) and regeneration. The paper starts by outlining the ways in which skeletal muscle can respond to damage resulting from exercise or other trauma. The age-related changes within skeletal muscle tissue and the host environment that may affect the proliferation and fusion of myoblasts in response to injury in old animals are explored. Finally, in vivo and in vitro data concerning the wide range of signaling molecules that stimulate satellite cells and other aspects of regeneration are discussed with respect to aging. Emphasis is placed on the important role of the host environment, inflammatory cells, growth factors and their receptors (particularly for FGF-2), and the extracellular matrix.

Theory of constraints

Abstract: A new approach to the management of production and operations was developed by Goldratt in the late 1970s. Now known as the theory of constraints (TOC), it provides a coherent management theory for running an organisation. It has two major components: a philosophy which underpins the working principle of on‐going improvement, and a generic approach for investigating, analysing and creating solutions to problems called the “thinking process” (TP). During the last decade several books and many articles have been published, and a journal has been established which deals solely with the TOC. This paper contains an analysis of a comprehensive list of publications on the TOC. Identified themes are classified as conceptual explanation and enhancement of the approach, comparison with other techniques based on its philosophy, and applications in areas such as production, purchasing, accounting, administration, education and quality management.

Comparison of PCR and Microscopy for Detection of Cryptosporidium parvum in Human Fecal Specimens: Clinical Trial

Abstract: PCR technology offers alternatives to conventional diagnosis of Cryptosporidium for both clinical and environmental samples. We compared microscopic examination by a conventional acid-fast staining procedure with a recently developed PCR test that can not only detect Cryptosporidium but is also able to differentiate between what appear to be host-adapted genotypes of the parasite. Examinations were performed on 511 stool specimens referred for screening on the basis of diarrhea. PCR detected a total of 36 positives out of the 511 samples, while routine microscopy detected 29 positives. Additional positives detected by PCR were eventually confirmed to be positive by microscopy. A total of five samples that were positive by routine microscopy at Western Diagnostic Pathology but negative by PCR and by microscopy in our laboratory were treated as false positives. Microscopy therefore exhibited 83.7% sensitivity and 98.9% specificity compared to PCR. PCR was more sensitive and easier to interpret but required more hands-on time to perform and was more expensive than microscopy. PCR, however, was very adaptable to batch analysis, reducing the costs considerably. Bulk buying of reagents and modifications to the procedure would decrease the cost of the PCR test even more. An important advantage of the PCR test, its ability to directly differentiate between different Cryptosporidium genotypes, will assist in determining the source of cryptosporidial outbreaks. Sensitivity, specificity, ability to genotype, ease of use, and adaptability to batch testing make PCR a useful tool for future diagnosis and studies on the molecular epidemiology of Cryptosporidium infections.