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Institution

Utsunomiya University

EducationUtsunomiya, Japan
About: Utsunomiya University is a education organization based out in Utsunomiya, Japan. It is known for research contribution in the topics: Laser & Holography. The organization has 4139 authors who have published 6812 publications receiving 91975 citations. The organization is also known as: Utsunomiya daigaku.


Papers
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Journal ArticleDOI
TL;DR: High-performance liquid chromatography analysis revealed that amiE functions as an AHL acylase, which hydrolyzes the amide bond of AHL, and the expression of AmiE in Pseudomonas aeruginosa PAO1 reduced AHL accumulation and elastase activity, which were regulated by AHL-mediated quorum sensing.
Abstract: Many Gram-negative bacteria use N-acyl-l-homoserine lactones (AHLs) as quorum-sensing signal molecules. We have reported that Acinetobacter strains isolated from activated sludge have AHL-degrading activity. In this study, we cloned the amiE gene as an AHL-degradative gene from the genomic library of Acinetobacter sp. strain Ooi24. High-performance liquid chromatography analysis revealed that AmiE functions as an AHL acylase, which hydrolyzes the amide bond of AHL. AmiE showed a high level of degrading activity against AHLs with long acyl chains but no activity against AHLs with acyl chains shorter than eight carbons. AmiE showed homology with a member of the amidases (EC 3.5.1.4) but not with any known AHL acylase enzymes. An amino acid sequence of AmiE from Ooi24 showed greater than 99% identities with uncharacterized proteins from Acinetobacter ursingii CIP 107286 and Acinetobacter sp. strain CIP 102129, but it was not found in the draft or complete genome sequences of other Acinetobacter strains. The presence of transposase-like genes around the amiE genes of these three Acinetobacter strains suggests that amiE is transferred by a putative transposon. Furthermore, the expression of AmiE in Pseudomonas aeruginosa PAO1 reduced AHL accumulation and elastase activity, which were regulated by AHL-mediated quorum sensing.

38 citations

Journal ArticleDOI
TL;DR: In this paper, the authors performed genomic comparisons between two Nitrospira strains (ND1 and NJ1 belonging to lineages I and II, respectively) previously isolated from activated sludge.
Abstract: The genus Nitrospira represents a dominant group of nitrite-oxidizing bacteria in natural and engineered ecosystems. This genus is phylogenetically divided into six lineages, for which vast phylogenetic and functional diversity has been revealed by recent molecular ecophysiological analyses. However, the genetic basis underlying these phenotypic differences remains largely unknown because of the lack of genome sequences representing their diversity. To gain a more comprehensive understanding of Nitrospira, we performed genomic comparisons between two Nitrospira strains (ND1 and NJ1 belonging to lineages I and II, respectively) previously isolated from activated sludge. In addition, the genomes of these strains were systematically compared with previously reported six Nitrospira genomes to reveal their similarity and presence/absence of several functional genes/operons. Comparisons of Nitrospira genomes indicated that their genomic diversity reflects phenotypic differences and versatile nitrogen metabolisms. Although most genes involved in key metabolic pathways were conserved between strains ND1 and NJ1, assimilatory nitrite reduction pathways of the two Nitrospira strains were different. In addition, the genomes of both strains contain a phylogenetically different urease locus and we confirmed their ureolytic activity. During gene annotation of strain NJ1, we found a gene cluster encoding a quorum-sensing system. From the enriched supernatant of strain NJ1, we successfully identified seven types of acyl-homoserine lactones with a range of C10-C14. In addition, the genome of strain NJ1 lacks genes relevant to flagella and the clustered regularly interspaced short palindromic repeat (CRISPR)-Cas (CRISPR-associated genes) systems, whereas most nitrifying bacteria including strain ND1 possess these genomic elements. These findings enhance our understanding of genomic plasticity and functional diversity among members of the genus Nitrospira.

38 citations

Journal ArticleDOI
TL;DR: When allophane was added to conventional complex media containing nutrient-derived inorganic phosphate, the production of nanaomycin and several other antibiotics, which are subject to phosphate regulation, was enhanced several fold with the simultaneous reduction of free phosphate.
Abstract: Nanaomycin production by Streptanyces rosa subsp. notoensis in complex media was inhibited by exogenously supplied inorganic phosphate. The inhibition was reversed by phosphate-trapping agents such as allophane and aluminum oxide. Under such condition nanaomycin production increased to the control level, and the phosphate content dropped down to the unsupplemented level. When allophane was added to conventional complex media containing nutrient-derived inorganic phosphate, the production of nanaomycin and several other antibiotics, which are subject to phosphate regulation, was enhanced several fold with the simultaneous reduction of free phosphate. The term "phosphate-depressed fermentation" is proposed for this technique.

38 citations

Journal ArticleDOI
TL;DR: The effect of plant attributes, such as the cell wall and fibrous tissue structure, on human digestion can be exploited to modify the digestive quality of processed foods as mentioned in this paper, and the modification of food matrices and structure design is also mentioned.
Abstract: The effect of plant attributes, such as the cell wall and fibrous tissue structure, on human digestion, can be exploited to modify the digestive quality of processed foods. To understand the digestive property of plant-based food, recent studies employing in vitro simulated digestion systems are highlighted and its relatedness to structural aspects are summarized in this review. The bioaccessibility and bioavailability of starch (as a digestible carbohydrate), lipids, proteins and functional phytochemicals, such as carotene, are mentioned as an indicator of plant-based food digestibility, including pasta as an example of plant-based processed foods. The modification of food matrices and structure design to control the bioavailability of pharmaceuticals and nutraceuticals is also mentioned.

38 citations

Journal ArticleDOI
TL;DR: The structure of orobanchol, a new germination stimulant isolated from red clover (Trifolium pratense), was proposed as 2a (tentative absolute configuration) on the basis of GC-MS comparison of the natural product with synthetic (±)-2a as discussed by the authors.

38 citations


Authors

Showing all 4148 results

NameH-indexPapersCitations
Kazuhito Hashimoto12078161195
Yoshinori Yamamoto8595028130
S. Uehara7860223493
Minghua Liu7467920727
Akira Fujishima7029969335
Satoshi Hasegawa6970822153
Donald A. Tryk6724025469
Hiromu Suzuki6525015241
Kunio Arai6429315022
Kazuo Suzuki6350717786
Jin Wang6019610435
James B. Reid6024611773
Richard L. Smith5930211420
Isao Kubo5830311291
Takao Yokota5724511813
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Performance
Metrics
No. of papers from the Institution in previous years
YearPapers
20236
202231
2021247
2020315
2019315
2018289