Institution
Utsunomiya University
Education•Utsunomiya, Japan•
About: Utsunomiya University is a education organization based out in Utsunomiya, Japan. It is known for research contribution in the topics: Laser & Holography. The organization has 4139 authors who have published 6812 publications receiving 91975 citations. The organization is also known as: Utsunomiya daigaku.
Topics: Laser, Holography, Polarization (waves), Plasma, Dielectric
Papers published on a yearly basis
Papers
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TL;DR: The improved AgarTrap method will enhance the molecular biological study of M. polymorpha and provide new possibilities for improving transformation techniques for a variety of plant species.
Abstract: Liverworts are key species for studies of plant evolution, occupying a basal position among the land plants. Marchantia polymorpha has emerged as a highly studied model liverwort, and many relevant techniques, including genetic transformation, have been established for this species. Agrobacterium-mediated transformation is widely used in many plant species because of its low cost. Recently, we developed a simplified Agrobacterium-mediated method for transforming M. polymorpha, known as AgarTrap (agar-utilized transformation with pouring solutions). The AgarTrap procedure, which involves culturing the liverwort tissue in various solutions on a single solid medium, yields up to a hundred independent transformants. AgarTrap is a simple procedure, requiring minimal expertise, cost, and time. Here, we investigated four factors that influence AgarTrap transformation efficiency: (1) humidity, (2) surfactant in the transformation buffer, (3) Agrobacterium strain, and (4) light/dark condition. We adapted the AgarTrap protocol for transforming intact gemmalings, achieving an exceptionally high transformation efficiency of 97%. The improved AgarTrap method will enhance the molecular biological study of M. polymorpha. Furthermore, this method provides new possibilities for improving transformation techniques for a variety of plant species.
33 citations
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TL;DR: Porcine in vitro production (IVP) yielded a high blastocyst rate and an excellent embryo quality when 3- to 4-cell and 5- to 8-cell stage embryos were selected on Day 2 after insemination and the same criteria yielded a higher quality of expanded blastocysts based on the stage of embryo development and morphology.
Abstract: The present study was conducted to determine the relationship between embryonic development speed at different stages (the cleaved stage at 52 h and the blastocyst stage at 6 days post insemination) and incidences of chromosome abnormalities in in vitro produced porcine embryos. Porcine oocytes were collected from 3-6-mm ovarian follicles obtained at a slaughterhouse and matured in modified NCSU-37 medium for 44-46 h. Following in vitro fertilization with a final concentration of 1 x 10(5) sperm/ml for 3 h, all oocytes were cultured in vitro for 52 h. Day-2 (52 h after insemination) embryos were classified according to their cleaved stages into 2-cell, 3- to 4-cell, 5- to 8-cell, and >8-cell stages; these were cultured separately for additional 4 days (Day 6). The resultant Day-6 blastocysts were classified according to the morphological diameter into 3 grades: Grade A, expanded blastocysts; Grade B, expanding blastocysts; and Grade C, early blastocysts. They were then analyzed chromosomally. The 3- to 4-cell and 5- to 8-cell embryos had significantly high blastocyst development rates (46.1 and 36.9%, respectively), and these blastocysts contained significantly more cells (40.2 and 42.4 cells, respectively) than those derived from 2-cell embryos and >8-cell embryos (28.6 and 26.5 cells, respectively). The incidence of chromosomal abnormalities was significantly higher in the blastocysts derived from 2-cell and >8-cell stage embryos than in the blastocysts derived from the other stage embryos. Furthermore, the grade A blastocysts had the lowest incidence of chromosomal abnormalities (35.3%) and contained the most cells (48.7 cells). Porcine in vitro production (IVP) yielded a high blastocyst rate and an excellent embryo quality when 3- to 4-cell and 5- to 8-cell stage embryos were selected on Day 2 after insemination. The same criteria yielded a higher quality of expanded blastocysts based on the stage of embryo development and morphology.
33 citations
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TL;DR: The observed relationship between snowpack depth and early mortality may be due to an indirect effect through the process of seed predation, and deep snow cover may reduce the success of rodents finding seeds in winter.
Abstract: The causes and timing of seed death in early regeneration process of Siebold's beech (Fagus crenata Blume) was studied at 15 sites along a snowfall gradient in Japan, in order to clarify why the seedling density of the species has geographic difference remarkably. Seed production did not significantly differ along the snowfall gradient. Pre-dispersal seed mortality by insect damage was higher at sites with light snowfall than at sites with heavy snowfall, but this only seemed to be a minor factor influencing the population. A large proportion of the viable nuts that fall in autumn ware killed in winter before germination. Winter mortality was much higher at sites with thin snow cover than that at sites with thick snow cover, and this factor was strongly correlated with the geographic variation of seedling regeneration probability. There was little seed mortality by winter desiccation. The main factor contributing to the geographic difference seemed to be a seed predation by rodents in winter. Deep snow cover may reduce the success of rodents finding seeds in winter. Thus the observed relationship between snowpack depth and early mortality may be due to an indirect effect through the process of seed predation.p>
32 citations
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TL;DR: The results suggest that somatostatin-associated feeding behavior is mediated by opioidergic mu- and adrenergic alpha-2-receptors in chicks.
32 citations
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TL;DR: A method of optimizing a computer-generated hologram based on parallel second harmonic generation is proposed for holographic femtosecond laser processing, which incorporates the width and spatial profile of the pulse into the hologram design.
Abstract: A method of optimizing a computer-generated hologram based on parallel second harmonic generation is proposed for holographic femtosecond laser processing. The method, which we call second harmonic optimization, incorporates the width and spatial profile of the pulse into the hologram design. With this method, we demonstrated parallel laser processing with high quality. Because of the enhanced processing accuracy, smaller structures were processed with a smaller energy than in our previous work. In parallel laser processing with 18 beams on a glass surface, the minimum average diameter of the processed structures was 271 nm when the mean fluence of the beams was 0.88 J/cm(2).
32 citations
Authors
Showing all 4148 results
Name | H-index | Papers | Citations |
---|---|---|---|
Kazuhito Hashimoto | 120 | 781 | 61195 |
Yoshinori Yamamoto | 85 | 950 | 28130 |
S. Uehara | 78 | 602 | 23493 |
Minghua Liu | 74 | 679 | 20727 |
Akira Fujishima | 70 | 299 | 69335 |
Satoshi Hasegawa | 69 | 708 | 22153 |
Donald A. Tryk | 67 | 240 | 25469 |
Hiromu Suzuki | 65 | 250 | 15241 |
Kunio Arai | 64 | 293 | 15022 |
Kazuo Suzuki | 63 | 507 | 17786 |
Jin Wang | 60 | 196 | 10435 |
James B. Reid | 60 | 246 | 11773 |
Richard L. Smith | 59 | 302 | 11420 |
Isao Kubo | 58 | 303 | 11291 |
Takao Yokota | 57 | 245 | 11813 |