Showing papers by "Wageningen University and Research Centre published in 1988"
TL;DR: The authors in this article found that the three strains of BCMV and B1 CMV-W have similar but non-identical molecular masses, which is in line with the biological distinction of NL3 in causing temperature-independent necrosis in bean cultivars with the necrosis gene.
Abstract: SUMMARY
In host-range studies, bean common mosaic virus strains (BCMV-NL1, -NL3 and -NY 15) usually induced distinct systemic symptoms in susceptible bean cultivars and latent infection in several Vigna genotypes (except NY15 which gave mosaic symptoms in the latter), while blackeye cowpea mosaic virus (B1CMV-W) caused distinct systemic symptoms in several Vigna genotypes and only weak systemic symptoms in a few bean genotypes only Biologically, B1CMV-W was closest to BCMV-NY15 and less close to -NL1 When using antisera to the three BCMV strains and five strains of B1CMV (including a strain originally considered cowpea aphid-borne mosaic virus CAMV-Mor) in SDS-immunodiffusion and ELISA, BCMV-NL1 and -NY15 were found to be closely related to each other and to BICMV-Fla, -NR and -W, and less closely to BICMV-Ind and -Mor Serological relationships of BCMV-NL1 and -NY15 to BCMV- NL3 were more distant, which is in line with the biological distinction of NL3 in causing temperature-independent necrosis in bean cultivars with the necrosis gene I PAGE analysis of coat proteins revealed that the three strains of BCMV and B1 CMV-W have similar but non-identical molecular masses Although molecular hybridisation may further elucidate quantitative relationships between potyvir-uses, variation within and among the potyviruses may continue to pose problems in their classification and identification
25 citations
TL;DR: The time-dependent fluorescence anisotropy of the two lipid probes in the sarcolemmal membranes showed, after an initial fast decay, a subsequent gradual increase, and this phenomenon was satisfactorily analyzed by assuming two populations of probe lipids with distinct lifetimes, rotational correlation times and molecular order.
Abstract: Phosphoinositide metabolism in the plasma membrane is linked to transmembrane signal transduction. In this study we have investigated some physical properties (e.g. molecular order and dynamics) of phosphatidylinositol (PI) in various membrane preparations by time-resolved fluorescence techniques, using a synthetic PI derivate with a cis-parinaroyl chain on the sn-2 position. Phospholipid vesicles, normal and denervated rat skeletal muscle sarcolemmal membranes, and acetylcholine receptor rich membrances from Torpedo marmorata were investigated both at 4°C and 20 °C. For comparison we have also included 2-parinaroyl-phosphatidylcholine (PC) in this study. The fluorescent lipids were incorporated into the membrane preparations by way of specific phospholipid transfer proteins, to ensure an efficient and non-perturbing insertion of the lipid-probes. In the Torpedo membranes the order parameters measured for the parinaroyl derivatives of both PC and PI were higher than in phospholipid vesicles. For the Torpedo membrane preparations the acyl chain order for the PI was lower than that for PC, whereas the opposite was true for the vesicles. This inversion strongly suggests that PI has different interactions with certain membrane components as compared to PC. This is also suggested by the significantly higher rate of restricted rotation of PI as compared to PC. In contrast to the order parameters, the correlation times were almost identical for both probes and showed little difference between vesicles and the Torpedo membranes. In contrast to Torpedo membranes, the time-dependent fluorescence anisotropy of the two lipid probes in the sarcolemmal membranes showed, after an initial fast decay, a subsequent gradual increase. This phenomenon was satisfactorily analyzed by assuming two populations of probe lipids with distinct lifetimes, rotational correlation times and molecular order. The order parameter of the population with a short lifetime compared with that of phospholipid vesicles, whereas the population with a long lifetime agreed with that of the Torpedo membranes.
20 citations
TL;DR: Symbolism is usually associated with religion or primitive cultures, and its pervasiveness is easily overlooked by natural resource professionals as mentioned in this paper, however, natural resource issues such as herbicide use, wild horse management, or forest dieback (Waldsterben) are rich in symbolic meaning that must be understood by competent professionals.
Abstract: Symbolism is usually associated with religion or primitive cultures, and its pervasiveness is easily overlooked by natural resource professionals This paper argues that natural resource issues such as herbicide use, wild horse management, or forest die‐back (Waldsterben) are rich in symbolic meaning that must be understood by competent professionals Theories of symbolism, developed in other fields, are applied to natural resources management in the Western world Examples are presented illustrating the magnitude, variety, and changing meaning of important natural resource symbols These examples focus on forest management, foresters, and the USDA‐Forest Service
8 citations
6 citations
TL;DR: In this paper, the authors investigated the electron transfer rate of three diphenyletioporphyrins covalently linked to an anthraquinone via a sulfonyloxy bridge at the ortho, meta or para substitution position of the phenyl groups of the porphyrin.
5 citations
TL;DR: It is concluded that selection for greasy fleeceweight in these lines has not altered the frequency ofK types but has been associated with an increase in the frequencyof the Hb Type B allele.
Abstract: Erythrocyte potassium (K) and haemoglobin (Hb) type polymorphisms were examined in ram and ewe hoggets and mature ewes from the Massey University fleeceweight-selected and control lines of Romney sheep There was no difference in the frequency of low potassium (LK) v high potassium (HK) phenotypes between the lines, and no difference in greasy hogget fleeceweight between the phenotypes Erythrocyte K+ concentration was positively related to fleeceweight in HK hoggets but no relationship existed in LK hoggets The frequency of Hb types differed significantly between the selection lines, the frequency of Type BB being greater in the fleeceweight-selected than control lines Hogget fleeceweights were significantly lowerin Type BB animals than in Type AA or AB animals It is concluded that selection for greasy fleeceweight in these lines has not altered the frequency ofK types but has been associated with an increase in the frequency of the Hb Type B allele However, this difference has most likely
2 citations