Institution
Wageningen University and Research Centre
Education•Wageningen, Netherlands•
About: Wageningen University and Research Centre is a education organization based out in Wageningen, Netherlands. It is known for research contribution in the topics: Population & Sustainability. The organization has 23474 authors who have published 54833 publications receiving 2608897 citations.
Topics: Population, Sustainability, European union, Agriculture, Climate change
Papers published on a yearly basis
Papers
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TL;DR: The development and validation of a method for the qualitative analysis of complex bifidobacterial communities based on PCR and denaturing gradient gel electrophoresis (DGGE) and revealed intragenomic 16S rDNA heterogeneity in the type strain of B. adolescentis, E-981074.
Abstract: We describe the development and validation of a method for the qualitative analysis of complex bifidobacterial communities based on PCR and denaturing gradient gel electrophoresis (DGGE). Bifidobacterium genus-specific primers were used to amplify an approximately 520-bp fragment from the 16S ribosomal DNA (rDNA), and the fragments were separated in a sequence-specific manner in DGGE. PCR products of the same length from different bifidobacterial species showed good separation upon DGGE. DGGE of fecal 16S rDNA amplicons from five adult individuals showed host-specific populations of bifidobacteria that were stable over a period of 4 weeks. Sequencing of fecal amplicons resulted in Bifidobacterium-like sequences, confirming that the profiles indeed represent the bifidobacterial population of feces. Bifidobacterium adolescentis was found to be the most common species in feces of the human adult subjects in this study. The methodological approach revealed intragenomic 16S rDNA heterogeneity in the type strain of B. adolescentis, E-981074. The strain was found to harbor five copies of 16S rDNA, two of which were sequenced. The two 16S rDNA sequences of B. adolescentis E-981074T exhibited microheterogeneity differing in eight positions over almost the total length of the gene.
446 citations
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TL;DR: It is concluded that extending the ecological network concept with multifunctional indicators is a promising step towards sustainable landscape development and stakeholder decision-making.
446 citations
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TL;DR: This review discusses how advances in mucus and mucin research, and insight in the microbial ecology promoted the understanding of mucin degradation, and recent insight is presented in mucin structure and organization.
Abstract: Mucins are a family of heavily glycosylated proteins that are the major organic components of the mucus layer, the protective layer covering the epithelial cells in many human and animal organs, including the entire gastro-intestinal tract. Microbes that can associate with mucins benefit from this interaction since they can get available nutrients, experience physico-chemical protection and adhere, resulting in increased residence time. Mucin-degrading microorganisms, which often are found in consortia, have not been extensively characterized as mucins are high molecular weight glycoproteins that are hard to study because of their size, complexity and heterogeneity. The purpose of this review is to discuss how advances in mucus and mucin research, and insight in the microbial ecology promoted our understanding of mucin degradation. Recent insight is presented in mucin structure and organization, the microorganisms known to use mucin as growth substrate, with a specific attention on Akkermansia muciniphila, and the molecular basis of microbial mucin degradation owing to availability of genome sequences.
446 citations
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TL;DR: Development of techniques for extraction, cleanup, separation, and sample storage that introduce minimal artifacts to increase the speed, sensitivity, and specificity of analytical techniques, as well as the development of techniques that can differentiate between abundant, naturally occurring particles, and manufactured nanoparticles are needed.
Abstract: Advances in the study of the environmental fate, transport, and ecotoxicological effects of engineered nanomaterials (ENMs) have been hampered by a lack of adequate techniques for the detection and quantification of ENMs at environmentally relevant concentrations in complex media. Analysis of ENMs differs from traditional chemical analysis because both chemical and physical forms must be considered. Because ENMs are present as colloidal systems, their physicochemical properties are dependent on their surroundings. Therefore, the simple act of trying to isolate, observe, and quantify ENMs may change their physicochemical properties, making analysis extremely susceptible to artifacts. Many analytical techniques applied in materials science and other chemical/biological/physical disciplines may be applied to ENM analysis as well; however, environmental and biological studies may require that methods be adapted to work at low concentrations in complex matrices. The most pressing research needs are the development of techniques for extraction, cleanup, separation, and sample storage that introduce minimal artifacts to increase the speed, sensitivity, and specificity of analytical techniques, as well as the development of techniques that can differentiate between abundant, naturally occurring particles, and manufactured nanoparticles.
446 citations
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Uppsala University1, University of Porto2, Cardiff University3, Katholieke Universiteit Leuven4, University of Groningen5, ETH Zurich6, University of Guelph7, Stockholm University8, Durham University9, University of British Columbia10, University of Tübingen11, Aalborg University12, University of Zurich13, Colorado State University14, Swedish University of Agricultural Sciences15, Karlstad University16, Radboud University Nijmegen17, University of Warsaw18, University of Turku19, University of Hawaii at Manoa20, Instituto Gulbenkian de Ciência21, University of Graz22, United States Department of Agriculture23, University of Freiburg24, University of Eastern Finland25, Wageningen University and Research Centre26, Spanish National Research Council27, Jagiellonian University28
TL;DR: Before the real-world conservation potential of genomic research can be realized, current infrastructures need to be modified, methods must mature, analytical pipelines need to been developed, and successful case studies must be disseminated to practitioners.
Abstract: The global loss of biodiversity continues at an alarming rate. Genomic approaches have been suggested as a promising tool for conservation practice as scaling up to genome-wide data can improve traditional conservation genetic inferences and provide qualitatively novel insights. However, the generation of genomic data and subsequent analyses and interpretations remain challenging and largely confined to academic research in ecology and evolution. This generates a gap between basic research and applicable solutions for conservation managers faced with multifaceted problems. Before the real-world conservation potential of genomic research can be realized, we suggest that current infrastructures need to be modified, methods must mature, analytical pipelines need to be developed, and successful case studies must be disseminated to practitioners.
446 citations
Authors
Showing all 23851 results
Name | H-index | Papers | Citations |
---|---|---|---|
Walter C. Willett | 334 | 2399 | 413322 |
Albert Hofman | 267 | 2530 | 321405 |
Frank B. Hu | 250 | 1675 | 253464 |
Willem M. de Vos | 148 | 670 | 88146 |
Willy Verstraete | 139 | 920 | 76659 |
Jonathan D. G. Jones | 129 | 417 | 80908 |
Bert Brunekreef | 124 | 806 | 81938 |
Pedro W. Crous | 115 | 809 | 51925 |
Marten Scheffer | 111 | 350 | 73789 |
Wim E. Hennink | 110 | 600 | 49940 |
Daan Kromhout | 108 | 453 | 55551 |
Peter H. Verburg | 107 | 464 | 34254 |
Marcel Dicke | 107 | 613 | 42959 |
Vincent W. V. Jaddoe | 106 | 1008 | 44269 |
Hao Wu | 105 | 669 | 42607 |